BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0119.3
(209 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein... 33 0.10
TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock... 30 0.66
BE999361 30 0.66
TC82663 similar to PIR|T51505|T51505 hypothetical protein F5E19_... 30 0.87
CB894695 similar to GP|22136262|gb| unknown protein {Arabidopsis... 30 0.87
TC78578 weakly similar to GP|10440402|dbj|BAB15734. FLJ00034 pro... 30 0.87
TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1 ... 29 1.1
BG584376 29 1.1
BF635925 similar to GP|8489806|gb chloroplast protein import com... 29 1.1
TC83503 weakly similar to PIR|T51851|T51851 RING-H2 finger prote... 29 1.5
TC79702 MtN14 29 1.5
AW691790 similar to GP|18491203|gb| unknown protein {Arabidopsis... 29 1.5
CA859009 homologue to PIR|S00703|S00 photosystem I protein A1 - ... 28 1.9
TC88434 weakly similar to PIR|G96548|G96548 hypothetical protein... 28 2.5
TC85306 similar to GP|21593320|gb|AAM65269.1 unknown {Arabidopsi... 28 2.5
AW127631 similar to PIR|T49904|T499 hypothetical protein T24H18.... 28 2.5
TC81513 28 2.5
TC83125 similar to PIR|T05841|T05841 spliceosome-associated prot... 27 4.3
AL385736 27 5.6
TC93536 weakly similar to GP|2961371|emb|CAA18118.1 hypothetical... 27 5.6
>TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein T28I19.100
- Arabidopsis thaliana, partial (14%)
Length = 1460
Score = 32.7 bits (73), Expect = 0.10
Identities = 28/120 (23%), Positives = 51/120 (42%), Gaps = 6/120 (5%)
Frame = +3
Query: 60 NHFDLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNATKI-VEESDIESEEEGRPTIIR 118
NH +F K + I +ET + ++K L+ K+ ++++ EEE I+
Sbjct: 267 NHDKKKEFDKNDTKLPIRTETDQ----ILKLGRKDLHPGKVEADKNEGHEEEEEDEHIVY 434
Query: 119 KKQIWHEKDTELDSEECAPSTSRSFDSIENLHELNKDAEEEDH-----FSHEREAMKNEV 173
Q E D + E +S +N+HE ++ +EE++ E E+ EV
Sbjct: 435 NMQNKREHDEQQQEGEEGNKHETEEESEDNVHERREEQDEEENKHGAEVQEENESKSEEV 614
>TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock protein
{Arabidopsis thaliana}, partial (89%)
Length = 1344
Score = 30.0 bits (66), Expect = 0.66
Identities = 23/77 (29%), Positives = 36/77 (45%), Gaps = 9/77 (11%)
Frame = +3
Query: 21 YTFTSFLFPALLFS--SSHYMGMKSH-------TSKEDDLKSHRGKLKNHFDLNKFPKIE 71
YT+T FL LL +MG+ + +K+DDLK KL + +K P +
Sbjct: 9 YTYTLFLLLLLLLLHLQIRFMGVDYYKVLQIDRNAKDDDLKKAYRKLAMKWHPDKNPNNK 188
Query: 72 DSFEIESETIEHSLDVI 88
E + +TI + DV+
Sbjct: 189KDAEAKFKTISEAYDVL 239
>BE999361
Length = 450
Score = 30.0 bits (66), Expect = 0.66
Identities = 16/28 (57%), Positives = 19/28 (67%)
Frame = +3
Query: 169 MKNEVMSMLQIYFAGVYFNGKYFLFLKQ 196
+KN M + IYF GVYF GK F FLK+
Sbjct: 291 IKNTYMLLKSIYFNGVYFLGK-FPFLKK 371
>TC82663 similar to PIR|T51505|T51505 hypothetical protein F5E19_70 -
Arabidopsis thaliana, partial (20%)
Length = 914
Score = 29.6 bits (65), Expect = 0.87
Identities = 22/86 (25%), Positives = 39/86 (44%)
Frame = +3
Query: 91 VEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLH 150
+EDS + +E ++E ++ + + EKD L++E+ A S ++ L
Sbjct: 171 LEDSERRLLMAKEENLEKSKKVEALESELETVKEEKDQALNNEQLAASHVQTL-----LE 335
Query: 151 ELNKDAEEEDHFSHEREAMKNEVMSM 176
E NK E D+ E E K + S+
Sbjct: 336 EKNKLINELDNSREEEEKSKKAMESL 413
>CB894695 similar to GP|22136262|gb| unknown protein {Arabidopsis thaliana},
partial (13%)
Length = 794
Score = 29.6 bits (65), Expect = 0.87
Identities = 21/92 (22%), Positives = 43/92 (45%), Gaps = 3/92 (3%)
Frame = +3
Query: 77 ESETIEHSLDVIKTVEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELDSEECA 136
+S + S+D V +SLN + +++ S+ P I+KKQ+ ++ S
Sbjct: 378 KSSGLNSSIDFDFNVSESLNLEESSSAAELFSDGRILPAEIKKKQVPLKQSLTTQSPSPN 557
Query: 137 PSTSRSFDSIENL---HELNKDAEEEDHFSHE 165
PS + S+ + N L K++ +E + ++
Sbjct: 558 PSLNPSYSTCNNESNGKNLRKESTKESKYLND 653
>TC78578 weakly similar to GP|10440402|dbj|BAB15734. FLJ00034 protein {Homo
sapiens}, partial (2%)
Length = 949
Score = 29.6 bits (65), Expect = 0.87
Identities = 25/108 (23%), Positives = 41/108 (37%), Gaps = 5/108 (4%)
Frame = +1
Query: 81 IEHSLDVIKTVEDSLNATKIVEESDI-----ESEEEGRPTIIRKKQIWHEKDTELDSEEC 135
I D +K + AT E+ + EEE IRK W + LD
Sbjct: 322 INARFDALKIKSNPAGATVSTSETQFRYESEDEEEEDEEVQIRKLIEWAKDSARLDPSPP 501
Query: 136 APSTSRSFDSIENLHELNKDAEEEDHFSHEREAMKNEVMSMLQIYFAG 183
+PS S D ++ + +++++DH + K + I AG
Sbjct: 502 SPSVS---DDEDHDPPSSDESDDDDHHRRRKPVKK*FYFILFAIIVAG 636
>TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1
{Arabidopsis thaliana}, partial (47%)
Length = 1538
Score = 29.3 bits (64), Expect = 1.1
Identities = 27/93 (29%), Positives = 44/93 (47%), Gaps = 5/93 (5%)
Frame = +3
Query: 47 KEDDLKSHRGK---LKNHFDLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNATKIVEE 103
+ DDL+ + GK + D+ K +I+ + ESE+ E + + + K E
Sbjct: 453 ENDDLEDY-GKPEEISEIIDVKKDKEIKQKAKSESESEESKESDSELRKKRRKSYKKSRE 629
Query: 104 SDIESEEEGR--PTIIRKKQIWHEKDTELDSEE 134
SD ESE E RK + + E D++ +SEE
Sbjct: 630 SDSESESESEVEDRKRRKSRKYSESDSDTNSEE 728
>BG584376
Length = 741
Score = 29.3 bits (64), Expect = 1.1
Identities = 20/67 (29%), Positives = 29/67 (42%), Gaps = 5/67 (7%)
Frame = -3
Query: 111 EGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLHE-----LNKDAEEEDHFSHE 165
E +PT I + + + + EE P T + ENL E +DAE+E + E
Sbjct: 700 ESKPTSIERTKAQTNEKAQKGEEEVEPKTIEKLVTKENLEEKIVGMSAEDAEKERNSKKE 521
Query: 166 REAMKNE 172
KNE
Sbjct: 520 EVEEKNE 500
>BF635925 similar to GP|8489806|gb chloroplast protein import component
Toc159 {Pisum sativum}, partial (6%)
Length = 389
Score = 29.3 bits (64), Expect = 1.1
Identities = 27/112 (24%), Positives = 46/112 (40%), Gaps = 5/112 (4%)
Frame = +1
Query: 71 EDSFEIESETIEHSL----DVIKTVEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEK 126
ED IE +E + D E+ N ++ EES++ E+G + E+
Sbjct: 16 EDGISIEGRAVEGEIESRVDGAVEEEEESNVVEVEEESNVVEVEDGSNV---DNVVAEEE 186
Query: 127 DTELDSEECAPSTSRSFDSIENLHELNKD-AEEEDHFSHEREAMKNEVMSML 177
++ +D S ++E E N D E + SH A++ E +ML
Sbjct: 187 ESNVDRVVEVEDESHVDTAVEEEAESNVDRVVEVEDGSHVDNAVEGEAEAML 342
>TC83503 weakly similar to PIR|T51851|T51851 RING-H2 finger protein RHB1a
[imported] - Arabidopsis thaliana, partial (44%)
Length = 835
Score = 28.9 bits (63), Expect = 1.5
Identities = 21/82 (25%), Positives = 38/82 (45%), Gaps = 3/82 (3%)
Frame = +1
Query: 84 SLDVIKTVEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTE---LDSEECAPSTS 140
S + + T ED +EESD++++ + P K ++W + ++ E+ P
Sbjct: 445 SFETLVTCED-------IEESDLKAQAKSAPISPTKAELWKSNELHALVIEEEDGCPICL 603
Query: 141 RSFDSIENLHELNKDAEEEDHF 162
+D +EN L+K E HF
Sbjct: 604 EEYD-VENPKTLSKC---EHHF 657
>TC79702 MtN14
Length = 973
Score = 28.9 bits (63), Expect = 1.5
Identities = 20/96 (20%), Positives = 49/96 (50%)
Frame = +2
Query: 77 ESETIEHSLDVIKTVEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDTELDSEECA 136
E++ +SL+V +T E+ + + V+E E E +++ + + E +T+ + +
Sbjct: 332 ETKENGNSLNVEETKENGDSVVEAVQEKPAEESET--VNVVKDENVVAEPETKDNVKTEE 505
Query: 137 PSTSRSFDSIENLHELNKDAEEEDHFSHEREAMKNE 172
S ++ + +E +++ EEE +++ + KNE
Sbjct: 506 TSEEKNEEKVEKEDAMDEKKEEEVITNNDAKIEKNE 613
>AW691790 similar to GP|18491203|gb| unknown protein {Arabidopsis thaliana},
partial (15%)
Length = 456
Score = 28.9 bits (63), Expect = 1.5
Identities = 24/101 (23%), Positives = 42/101 (40%), Gaps = 6/101 (5%)
Frame = +3
Query: 8 NMQYIYRVAPNGIYTFTSFLFPALLFSSSHYMGMKSHTSKED------DLKSHRGKLKNH 61
N+ I + I T SF F L S S S TS+ + LK+ KNH
Sbjct: 42 NISLILKCYFTFIITCISFFFFLNLNSLSFLKSFPSWTSENEMKHQVLSLKTFSKLFKNH 221
Query: 62 FDLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNATKIVE 102
F + +I + +++ ++ ++ +ED L+ + E
Sbjct: 222 FWIGLLCEIHTRMSMVRKSLSSRVENVEEIEDKLSLLDLPE 344
>CA859009 homologue to PIR|S00703|S00 photosystem I protein A1 - garden pea
chloroplast, partial (16%)
Length = 823
Score = 28.5 bits (62), Expect = 1.9
Identities = 15/61 (24%), Positives = 32/61 (51%), Gaps = 7/61 (11%)
Frame = +2
Query: 144 DSIENLHELNKDAEEEDHFSHEREAMKNEV-------MSMLQIYFAGVYFNGKYFLFLKQ 196
D+ + L+ DA + D + + E + +V +S++ ++ +G+YF+G F +K
Sbjct: 599 DTTTWIWNLHADAHDFDSHTSDLEEISRKVFSAHFGQLSIIFLWLSGMYFHGARFSIMKH 778
Query: 197 G 197
G
Sbjct: 779 G 781
>TC88434 weakly similar to PIR|G96548|G96548 hypothetical protein F23H24.4
[imported] - Arabidopsis thaliana, partial (38%)
Length = 1274
Score = 28.1 bits (61), Expect = 2.5
Identities = 26/83 (31%), Positives = 36/83 (43%), Gaps = 6/83 (7%)
Frame = +1
Query: 82 EHSLDVIKTVE-DSLNATKIVEESDIESEEEGRP-----TIIRKKQIWHEKDTELDSEEC 135
E V + V+ + LNA EE D E EEE IIR + + + ++
Sbjct: 307 EEEQQVFRRVKRERLNAAAATEEKDEEEEEEENQDSSIRRIIRSEFFKLKSLINEEKDDL 486
Query: 136 APSTSRSFDSIENLHELNKDAEE 158
S FDSI LHE +K E+
Sbjct: 487 MNGDSEKFDSI--LHEFDKLHEQ 549
>TC85306 similar to GP|21593320|gb|AAM65269.1 unknown {Arabidopsis
thaliana}, partial (24%)
Length = 876
Score = 28.1 bits (61), Expect = 2.5
Identities = 25/109 (22%), Positives = 50/109 (44%), Gaps = 5/109 (4%)
Frame = +2
Query: 69 KIEDSFEIESETIEHSLDVIKTVEDSLNATKIVEESDIESEEEGRPTIIRKKQIWHEKDT 128
++ED E + +T+E + E+ L ++ E +ES G ++ + D
Sbjct: 173 EVEDGKEFDVDTVEAKKRKLDGKEEQLAKEQVKEMKKLESFLFG--SLYSPPEFGKGDDD 346
Query: 129 ELDSEECAPS-----TSRSFDSIENLHELNKDAEEEDHFSHEREAMKNE 172
E+D+ A + T RS DS+ +++ + D +E S +A+K +
Sbjct: 347 EVDAAASATASNLFFTDRSADSVLTVYQEDADFSDE---SDNDDALKRK 484
>AW127631 similar to PIR|T49904|T499 hypothetical protein T24H18.70 -
Arabidopsis thaliana, partial (2%)
Length = 496
Score = 28.1 bits (61), Expect = 2.5
Identities = 18/80 (22%), Positives = 34/80 (42%)
Frame = +3
Query: 38 YMGMKSHTSKEDDLKSHRGKLKNHFDLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNA 97
+ ++SH DD KS G NH + + +S+ + +SL+ K+V
Sbjct: 45 FTNLESHICPVDDTKSVTGSCANHITVLQTSPTNESYRCKR---RNSLEDSKSVSSCNKC 215
Query: 98 TKIVEESDIESEEEGRPTII 117
+ S++ES + + I
Sbjct: 216 NPAIITSELESARNSKSSNI 275
>TC81513
Length = 441
Score = 28.1 bits (61), Expect = 2.5
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +3
Query: 169 MKNEVMSMLQIYFAGVYFNGKYFLFLKQ 196
+KN M + IYF G YF GK F F+K+
Sbjct: 219 IKNNYMILKSIYFNGAYFLGK-FPFIKK 299
>TC83125 similar to PIR|T05841|T05841 spliceosome-associated protein homolog
F17L22.120 - Arabidopsis thaliana, partial (24%)
Length = 722
Score = 27.3 bits (59), Expect = 4.3
Identities = 30/140 (21%), Positives = 63/140 (44%), Gaps = 6/140 (4%)
Frame = +2
Query: 40 GMKSHTSKEDDLKSHRGKLKNHFDLNKFPKIEDSFEIESETIEHSLDVIKTVEDSLNATK 99
G+ + SKE+D + RGK K + ++ P + ES+ + + D K V + +
Sbjct: 74 GVVAKKSKENDRRRRRGKAKKNNKASEQP--ASNIGEESDNAKENTDP-KQVFEQVEIEY 244
Query: 100 IVEESDI-ESEEEGRPTIIRKKQIW-----HEKDTELDSEECAPSTSRSFDSIENLHELN 153
+ E+ D+ E +E I K E D + +EE A + ++ + E N
Sbjct: 245 VPEKVDLYEGMDEEFRKIFEKFSFTEVAASEETDKKDVAEETAATKKKANSDSDYEDEEN 424
Query: 154 KDAEEEDHFSHEREAMKNEV 173
+ ++E S++++ ++ +
Sbjct: 425 DNEQKEKGVSNKKKKLQRRM 484
>AL385736
Length = 502
Score = 26.9 bits (58), Expect = 5.6
Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Frame = -3
Query: 115 TIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLHELNKDAEEEDHFSHEREA---MKN 171
T+IRKK +W ++ EEC F+ I L + + +D S+ R + N
Sbjct: 407 TVIRKKVVWSLEEYHTIMEECVKRKIADFEKI--LRHVEPNLVFKDPQSNSRVSAIDYVN 234
Query: 172 EVMSML 177
EV +M+
Sbjct: 233 EVKNMI 216
>TC93536 weakly similar to GP|2961371|emb|CAA18118.1 hypothetical protein
(fragment) {Arabidopsis thaliana}, partial (58%)
Length = 815
Score = 26.9 bits (58), Expect = 5.6
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +2
Query: 107 ESEEEGRPTIIRKKQIWHEKDTELDSEECAPSTSRSFDSIENLHELNKDAEEEDH 161
ESE++ + I+ K Q W + EL++E +H+L+K EE H
Sbjct: 515 ESEQKIQEVILMKSQQWEKFKLELEAE---------------IHKLDKGLREEAH 634
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,100,006
Number of Sequences: 36976
Number of extensions: 78947
Number of successful extensions: 382
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 377
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 379
length of query: 209
length of database: 9,014,727
effective HSP length: 92
effective length of query: 117
effective length of database: 5,612,935
effective search space: 656713395
effective search space used: 656713395
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0119.3