BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0113.5
(992 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR re... 118 1e-26
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 102 9e-22
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 82 1e-15
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 69 1e-11
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 44 3e-04
BG586283 similar to GP|7267666|em RNA-directed DNA polymerase-li... 39 0.010
TC82093 38 0.022
AL372330 similar to GP|21322752|dbj cold shock protein-1 {Tritic... 33 0.54
AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase... 31 2.1
TC81468 similar to GP|20259431|gb|AAM14036.1 unknown protein {Ar... 31 2.7
TC93012 similar to GP|10177104|dbj|BAB10438. gene_id:MXC20.13~un... 30 4.6
BQ751233 similar to GP|18307448|em probable low-affinity hexose ... 30 4.6
AL371050 30 4.6
TC83050 similar to SP|Q9FGZ4|WR48_ARATH Probable WRKY transcript... 30 6.0
TC80486 similar to GP|11994617|dbj|BAB02754. contains similarity... 29 7.8
TC78961 similar to GP|18252179|gb|AAL61922.1 unknown protein {Ar... 29 7.8
AL385742 weakly similar to GP|12597813|gb hypothetical protein {... 29 7.8
>TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 798
Score = 118 bits (296), Expect = 1e-26
Identities = 78/269 (28%), Positives = 121/269 (43%), Gaps = 15/269 (5%)
Frame = -1
Query: 623 RNFLWQELQNIASQVTKPWVTVGDFNTYLQASDKMGGGPPNLLSMAKFRDCLDACSLSDL 682
R LW + NI +Q W +GDFNT L + + G P L M F+ D +L L
Sbjct: 795 RRQLWSAISNIQTQHKLSWCCIGDFNTILGSHEHQGSHTPARLPMLDFQQWSDVNNLFHL 616
Query: 683 GFKGPPFTW----KGR-GVKERIDWALGNARWISSFPEATVLHLPKLKYDHKPILIQLGP 737
+G FTW +GR ++R+D ++ N I + + L KL+ DH PIL +L
Sbjct: 615 PTRGSAFTWTNGRRGRNNTRKRLDRSIVNQLMIDNCESLSACTLTKLRSDHFPILFELQT 436
Query: 738 EHMDSSQRPFRFLAAWLTHEDFPRLVKSSWDSHHDSWIPASEAFC---------EEAASW 788
+++ S F+F+ W H D ++K W + + F E W
Sbjct: 435 QNIQFSS-SFKFMKMWSAHPDCINIIKQCWANQ----VVGCPMFVLNQKLKNLKEVLKVW 271
Query: 789 NRDVFGEIGRTKRRLMRRLEGINNHL-RMSHSPYLEKLQKRLWKEYQTVLIREELLWRQK 847
N++ FG + + L+ I + + +S L +K ++ L EE+ W +K
Sbjct: 270 NKNTFGNVHSQVDNAYKELDDIQVKIDSIGYSDVLMDQEKAAQLNLESALNIEEVFWHEK 91
Query: 848 SRVNWLNHGDRNTHFFHTTTMVHRKRNKI 876
S+VNW GDRNT FFH + R + I
Sbjct: 90 SKVNWHCEGDRNTAFFHRVAKIKRTSSLI 4
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 102 bits (253), Expect = 9e-22
Identities = 57/158 (36%), Positives = 85/158 (53%), Gaps = 1/158 (0%)
Frame = +3
Query: 822 LEKLQKRLWKEYQTVLIREELLWRQKSRVNWLNHGDRNTHFFHTTTMVHRKRNKIEALNN 881
L L+ L +EY EE+ W QKSR+NWL GDRNT FFH T R +N+I +L +
Sbjct: 81 LSLLRSELNEEYHN----EEIFWMQKSRLNWLRSGDRNTKFFHAVTKNRRAQNRILSLID 248
Query: 882 DVGELITDQDQLRSMAVTSFQNLYTSPGPGEPYHVRNAFPSLPRETLD-EIMGPLEAAEI 940
D + ++ L +A + F+ LY+S G N+ P++ E + ++M + E+
Sbjct: 249 DDDKEWFVEEDLGRLADSHFKLLYSSEDVGITLEDWNSIPAIVTEEQNAQLMAQISREEV 428
Query: 941 RVAVFSMGAMKAPGPDDLNPLFFQSQWQVIGPSVVKLA 978
R AVF + K PGPD +N FFQ W +G + +A
Sbjct: 429 REAVFDINPHKCPGPDGMNVFFFQQFWDTMGDDLTSMA 542
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 82.0 bits (201), Expect = 1e-15
Identities = 45/150 (30%), Positives = 72/150 (48%), Gaps = 12/150 (8%)
Frame = +2
Query: 852 WLNHGDRNTHFFHTTTMVHRKRNKIEALNNDVGELITDQDQLRSMAVTSFQNLYTSPGPG 911
WL GD+NT FFH+ RK N+I+ L ++ G ++ + + +T F NL+TS
Sbjct: 5 WLKDGDKNTKFFHSKASQRRKVNEIKKLKDETGNWCKGEENVERLLITYFNNLFTSSN-- 178
Query: 912 EPYHVRNAFPSLPRETLDEIMGPLE------------AAEIRVAVFSMGAMKAPGPDDLN 959
P+ ET + + G L E+ A+ M +KAPGPD L
Sbjct: 179 ---------PTAIEETCEVVKGKLSHEHIVWCEKEFTEEEVLEAINQMHPVKAPGPDGLP 331
Query: 960 PLFFQSQWQVIGPSVVKLAMTVYSNRRKSK 989
LFFQ W ++G V ++ + V +N +++
Sbjct: 332 ALFFQKYWHIVGKEVQQMVLQVLNNSMETE 421
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 68.6 bits (166), Expect = 1e-11
Identities = 44/142 (30%), Positives = 70/142 (48%), Gaps = 1/142 (0%)
Frame = +2
Query: 825 LQKRLWKEYQTVLIREELLWRQKSRVNWLNHGDRNTHFFHTTTMVHRKRNKIEALNNDVG 884
L+++L + Y+ EE W+QKSR W GD N F+H T RN+I L + G
Sbjct: 8 LKEKLQEAYKD----EEDYWQQKSRNMWHISGDLNKKFYHALTKQRHARNRIVGLYDYDG 175
Query: 885 ELITDQDQLRSMAVTSFQNLYTSPGP-GEPYHVRNAFPSLPRETLDEIMGPLEAAEIRVA 943
IT++ + +AV F++L+ P G + S+ + ++ E+R+A
Sbjct: 176 NWITEEQGVEKVAVDYFEDLFQRTTPTGFDGFLDEITSSITPQMNQRLLRLATEEEVRLA 355
Query: 944 VFSMGAMKAPGPDDLNPLFFQS 965
+F M KAPGPD + L F +
Sbjct: 356 LFIMHPEKAPGPDGMTTLLFST 421
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 43.9 bits (102), Expect = 3e-04
Identities = 20/47 (42%), Positives = 28/47 (59%)
Frame = +3
Query: 929 DEIMGPLEAAEIRVAVFSMGAMKAPGPDDLNPLFFQSQWQVIGPSVV 975
D + A E++ A+FSM + KAPG D N FF+ W +IG SV+
Sbjct: 6 DLLCSEFTAVEVKNALFSMDSSKAPGIDGYNVHFFKCSWNIIGDSVI 146
>BG586283 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (7%)
Length = 774
Score = 38.9 bits (89), Expect = 0.010
Identities = 18/44 (40%), Positives = 22/44 (49%)
Frame = +3
Query: 616 GSPNASIRNFLWQELQNIASQVTKPWVTVGDFNTYLQASDKMGG 659
G P R W EL I +Q W+ GDFN L S+K+GG
Sbjct: 519 GVPQPEHRAKFWDELSTIGAQRDGAWLLTGDFNDLLDNSEKVGG 650
>TC82093
Length = 569
Score = 37.7 bits (86), Expect = 0.022
Identities = 15/29 (51%), Positives = 21/29 (71%)
Frame = -1
Query: 836 VLIREELLWRQKSRVNWLNHGDRNTHFFH 864
+L + E+LW KS+ NW+ +G RNT FFH
Sbjct: 476 LLSQNEMLWY*KSQ*NWVKYGSRNTWFFH 390
>AL372330 similar to GP|21322752|dbj cold shock protein-1 {Triticum
aestivum}, partial (33%)
Length = 435
Score = 33.1 bits (74), Expect = 0.54
Identities = 19/60 (31%), Positives = 32/60 (52%), Gaps = 5/60 (8%)
Frame = +1
Query: 798 RTKRRLMRRLEGINNHLRMSHSPYLEKLQKRLWKE---YQTV--LIREELLWRQKSRVNW 852
R +RR RRL I LR ++Q+R+W+ ++++ + R L WRQ+ R+ W
Sbjct: 100 RLRRRFERRLRRIQRRLR--------RIQRRIWRRRVWWRSIRRIWRRRLRWRQRRRIWW 255
>AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (4%)
Length = 305
Score = 31.2 bits (69), Expect = 2.1
Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 2/53 (3%)
Frame = +1
Query: 939 EIRVAVFSMGAMKA--PGPDDLNPLFFQSQWQVIGPSVVKLAMTVYSNRRKSK 989
E+R AV+ + + PGPD +N F + W++I +++ M ++N + K
Sbjct: 133 EVRKAVWDCDSSHS*NPGPDGVNFTFVKEYWELIKVDFLRVVMEFHTNGKLGK 291
>TC81468 similar to GP|20259431|gb|AAM14036.1 unknown protein {Arabidopsis
thaliana}, partial (38%)
Length = 1172
Score = 30.8 bits (68), Expect = 2.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -1
Query: 746 PFRFLAAWLTHEDFPRLVKSSWDSHHDSWIPASEAFCEEAAS 787
P R+L W HE L++ + ++HH S P+ + CE S
Sbjct: 482 PLRYL--WQAHEHSQILLQHAHEAHHQSQTPSKVSLCEHQES 363
>TC93012 similar to GP|10177104|dbj|BAB10438. gene_id:MXC20.13~unknown
protein {Arabidopsis thaliana}, partial (18%)
Length = 710
Score = 30.0 bits (66), Expect = 4.6
Identities = 20/66 (30%), Positives = 30/66 (45%)
Frame = +1
Query: 591 SHRQFVHLRIHPKNNVPNFLVTFVYGSPNASIRNFLWQELQNIASQVTKPWVTVGDFNTY 650
SH + +IHPK+ + + T S SI +F + L N+ + F TY
Sbjct: 46 SHLSKLSSKIHPKSKIEETMET----SHPLSIESFSYSWLVNLKPSSLVESLDGSSFRTY 213
Query: 651 LQASDK 656
L ASD+
Sbjct: 214 LDASDE 231
>BQ751233 similar to GP|18307448|em probable low-affinity hexose transporter
HXT3 {Neurospora crassa}, partial (30%)
Length = 659
Score = 30.0 bits (66), Expect = 4.6
Identities = 25/90 (27%), Positives = 38/90 (41%), Gaps = 4/90 (4%)
Frame = +1
Query: 378 SGPTKITSAPTGPQPHDGGTGTKVNPNSQGAIPQQSRADVAHPSHFKSGQHDKLGPTSGS 437
SGP ++S+ + P P + + V+ + P S + PS L P+S +
Sbjct: 412 SGPASVSSSRSRPSPPGTSSPSAVSSTVSASAPSPSSSPCTSPS--------PLRPSSAA 567
Query: 438 SSLKKTKM---KARGHQR-VEGSSEPRRSP 463
SS + T A G R V G+ PR P
Sbjct: 568 SSSRPTSSSSPSASGPPRWVTGAPRPRPVP 657
>AL371050
Length = 426
Score = 30.0 bits (66), Expect = 4.6
Identities = 23/84 (27%), Positives = 34/84 (40%), Gaps = 2/84 (2%)
Frame = +3
Query: 317 VQIPEQNNLNQNTPTNQESQPQHMVDSAGSGI--SPTKGKVVNTNDEPNHEGISSVPNDE 374
+ +P N + +PTN + P + + + SPT G VV +
Sbjct: 225 ITVPSTNPVTTVSPTNPGATPVTVPSTTPPSVPLSPTNGGVV----------------EG 356
Query: 375 TMISGPTKITSAPTGPQPHDGGTG 398
T+ G T + S P P P GGTG
Sbjct: 357 TVTGGTTPVNSYPP-PSPLSGGTG 425
>TC83050 similar to SP|Q9FGZ4|WR48_ARATH Probable WRKY transcription factor
48 (WRKY DNA-binding protein 48). [Mouse-ear cress],
partial (31%)
Length = 974
Score = 29.6 bits (65), Expect = 6.0
Identities = 15/38 (39%), Positives = 19/38 (49%)
Frame = +2
Query: 339 HMVDSAGSGISPTKGKVVNTNDEPNHEGISSVPNDETM 376
H + S GS P +V NT PN ISS N+ T+
Sbjct: 275 HTLPSPGSSNIPDSSEVFNTPVSPNSSSISSSSNEATV 388
>TC80486 similar to GP|11994617|dbj|BAB02754. contains similarity to kinesin
protein~gene_id:MGL6.9 {Arabidopsis thaliana}, partial
(15%)
Length = 741
Score = 29.3 bits (64), Expect = 7.8
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = -2
Query: 100 HGALSGNPTMETNVHSLKRRTDKSCSVVQSSKLPCGVLWPRSVTGNWQER 149
H A++ +P +V +L R S ++ LPCG +WP + T W+ R
Sbjct: 311 HRAMTASPA---SVDALCREPPAG*S*TAAAALPCGGIWP-ATTWRWERR 174
>TC78961 similar to GP|18252179|gb|AAL61922.1 unknown protein {Arabidopsis
thaliana}, partial (71%)
Length = 974
Score = 29.3 bits (64), Expect = 7.8
Identities = 31/108 (28%), Positives = 42/108 (38%)
Frame = +2
Query: 389 GPQPHDGGTGTKVNPNSQGAIPQQSRADVAHPSHFKSGQHDKLGPTSGSSSLKKTKMKAR 448
GP P G+G N G + +A ++ G+ + +S KK AR
Sbjct: 335 GPPP---GSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSP-KKLSRHAR 502
Query: 449 GHQRVEGSSEPRRSPNASSNAPGDMGGRTFRERDRSLSPRKHRSS*RA 496
R G S RSP S + R R RDRS SP + S R+
Sbjct: 503 SVSRSPGRS---RSPARSRSPARSRSPRRGRSRDRSYSPARSYSRSRS 637
>AL385742 weakly similar to GP|12597813|gb hypothetical protein {Arabidopsis
thaliana}, partial (7%)
Length = 493
Score = 29.3 bits (64), Expect = 7.8
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 4/65 (6%)
Frame = -1
Query: 88 LCSCLFRGTLGHH----GALSGNPTMETNVHSLKRRTDKSCSVVQSSKLPCGVLWPRSVT 143
LC+ L L H+ G + + SL R+ SCS QS +L ++ PRS
Sbjct: 391 LCTLLIAARLAHYLHSIELNCGPKLVVLQLFSLVDRSSISCSCKQSLELTFRIITPRSTL 212
Query: 144 GNWQE 148
+W +
Sbjct: 211 CSWNQ 197
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.141 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,959,769
Number of Sequences: 36976
Number of extensions: 538747
Number of successful extensions: 3180
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 2363
Number of HSP's successfully gapped in prelim test: 116
Number of HSP's that attempted gapping in prelim test: 767
Number of HSP's gapped (non-prelim): 2556
length of query: 992
length of database: 9,014,727
effective HSP length: 106
effective length of query: 886
effective length of database: 5,095,271
effective search space: 4514410106
effective search space used: 4514410106
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0113.5