BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0106.9
(458 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87745 similar to PIR|F84452|F84452 probable nonsense-mediated ... 501 e-179
TC79383 weakly similar to GP|12643043|gb|AAK00432.1 hypothetical... 166 1e-41
BE318443 73 8e-17
BG447717 42 4e-04
BF635945 32 0.39
TC85803 similar to GP|13785471|dbj|BAB43909. phosphoenolpyruvate... 30 1.9
TC85752 similar to GP|21706788|gb|AAH34388.1 Unknown (protein fo... 30 1.9
TC78790 similar to PIR|T45678|T45678 hypothetical protein F14P22... 29 3.3
TC76645 similar to PIR|S16294|S16294 chlorophyll a/b-binding pro... 28 5.7
CA919710 similar to GP|10177798|dbj emb|CAB87778.1~gene_id:MXA21... 28 9.7
BQ151358 similar to GP|11993889|gb| virion-associated nuclear-sh... 28 9.7
>TC87745 similar to PIR|F84452|F84452 probable nonsense-mediated mRNA decay
protein [imported] - Arabidopsis thaliana, partial (77%)
Length = 1902
Score = 501 bits (1290), Expect(2) = e-179
Identities = 244/329 (74%), Positives = 278/329 (84%), Gaps = 3/329 (0%)
Frame = +3
Query: 9 MEIEAGMFSVPQTICSVLCCMCGIPMQPNAANMCVKCLSSQEDITEGLQKHINIFHCPQC 68
M EAGMF V QTI SVLCC CGI MQPNAANMCVKCL S+ DITEGL K + + HCP+C
Sbjct: 105 MAQEAGMFKVHQTIGSVLCCKCGIRMQPNAANMCVKCLRSEVDITEGLLKRLVLVHCPEC 284
Query: 69 ESYFQHPKTWIKHLKPESDELLKFCLKMMKKT---NKVRMVDARFIWTEPHSKRIKINVK 125
ESY Q P+TW+K L+ ES ELL FCLK ++K NK ++V+A FIWTEPHSKR+K+ V
Sbjct: 285 ESYLQPPRTWVK-LQLESKELLTFCLKKLQKNMNINKAKLVNAEFIWTEPHSKRVKVKVS 461
Query: 126 VQKEVLNGVILEQSYLVEYVQLEHLCDSCTRVAANPDQWVAVVQLRQHVSHRRTFFYLEQ 185
VQKEV +G ILEQSY VEYVQ +H+C+SC+RVAANPDQW+A VQLRQHVSHRRTF+YLEQ
Sbjct: 462 VQKEVYHGAILEQSYPVEYVQQDHMCESCSRVAANPDQWIAAVQLRQHVSHRRTFYYLEQ 641
Query: 186 LILKHGAAARAIRIKQMHEGIDFYFSNEDHGEKFVQFIGKVAPIRSKGYNKQLVSHDTKS 245
LIL+HGAAARAIRIKQM GIDFYFSN HG KFV+F+GKVAP++S+ +KQLVSHD+KS
Sbjct: 642 LILRHGAAARAIRIKQMDHGIDFYFSNRSHGIKFVEFVGKVAPVKSRS-DKQLVSHDSKS 818
Query: 246 NNYNYRYTFSVEISPICREDLICLPHHVALSLGNLGPLVICTKVTNSIVLLDPFTLRHCF 305
NNYNY+YTFSVEISPICREDLICLP A LGN+GP+VICTKVTNSI LLDP TL+ F
Sbjct: 819 NNYNYKYTFSVEISPICREDLICLPPKAAAILGNIGPIVICTKVTNSIALLDPLTLKFGF 998
Query: 306 LDASQYWRASFKSLLTSRQLVEYVVLDVE 334
LDA QYWR SFKSLLTSRQLVEY+VLDVE
Sbjct: 999 LDAEQYWRTSFKSLLTSRQLVEYIVLDVE 1085
Score = 144 bits (363), Expect(2) = e-179
Identities = 74/110 (67%), Positives = 87/110 (78%)
Frame = +2
Query: 349 YVLAVAQVARVSDFGKNDTIFSIKTHLGHLLNPGDYALGYDLYGANCNDIELDKYKGGDL 408
Y LA AQVARVSDFGKNDT+FSI+THLGHLL PGD ALGYDLY AN ND+ELD+YK G++
Sbjct: 1124 YALADAQVARVSDFGKNDTMFSIRTHLGHLLKPGDNALGYDLYAANSNDMELDRYK-GNI 1300
Query: 409 PHAILVKKSYVEKRLKKRGKPRSWKLKSLNMEIDDKGRTDPDKMSSEYEL 458
P +L+KKSY EKR K RS+KLK + ME DDK + + DK S+EYEL
Sbjct: 1301 PEVVLIKKSYPEKR-----KTRSFKLKRIPMEADDKIKVNEDKRSAEYEL 1435
>TC79383 weakly similar to GP|12643043|gb|AAK00432.1 hypothetical protein
{Oryza sativa}, partial (8%)
Length = 1082
Score = 166 bits (421), Expect = 1e-41
Identities = 109/267 (40%), Positives = 141/267 (51%), Gaps = 32/267 (11%)
Frame = +2
Query: 156 RVAANPDQWVAVVQLRQ-HVSHRRTFFYLEQL-ILKH-GAAARAIRIK-QMHEGIDFYFS 211
+V+ + + AVV +RQ HVS RT F+LEQ+ + H G RI+ ++EG+ FYF+
Sbjct: 5 KVSKMEEGFCAVVNVRQQHVSELRTLFHLEQVSVWNHLGIVGNFRRIEYNINEGLSFYFN 184
Query: 212 NEDHGEKFVQFIGKVAPIRSKGYNKQLVSHDTKSNNYNYRYTFSVEISPICREDLICLPH 271
+ + V I + P + + N Y FSVEI PIC DLI LP
Sbjct: 185 SLSDAAEVVNLIVDILPAQVQW-------------NKIDPYLFSVEIVPICCGDLIILPS 325
Query: 272 HVALSLG-NLGPLVICTKVTNSIVLLDPFTLRHCFLDASQYWRASFKSLLTSRQLVEYVV 330
++A SLG N+GP+VICT+V + LLDPFTL HCFL A QYW A F + QLVEY V
Sbjct: 326 NIAASLGPNIGPIVICTRVAKTFTLLDPFTLTHCFLKAGQYWDAPFTPSFSRPQLVEYAV 505
Query: 331 LDVEEVVPSE---------------------------VVTVGGTKYVLAVAQVARVSDFG 363
L +E++ E T KY LA A VARV D G
Sbjct: 506 LGIEKLDEEEEEEVEENKQKKKLVFKDEHDEDGDAFATYTAAAKKYRLANAIVARVKDIG 685
Query: 364 KNDTIFSIKTHLGHLLNPGDYALGYDL 390
N+ F I+THLG +L PGD+ALGYDL
Sbjct: 686 NNNHTFQIRTHLGRILKPGDHALGYDL 766
>BE318443
Length = 502
Score = 73.2 bits (178), Expect(2) = 8e-17
Identities = 55/145 (37%), Positives = 79/145 (53%), Gaps = 10/145 (6%)
Frame = +2
Query: 164 WVAVVQLRQ-HVSHRRTFFYLEQL-ILKHGAAARAIR--IKQMHEGIDFYFSNEDHGEKF 219
W +VV++RQ HVS RT F+LEQ+ ++ + + I + +G F F+ F
Sbjct: 20 WRSVVKVRQQHVSQLRTVFHLEQISVMTNLGILDDVEKIIYDVDQGFSFCFNIISCAWYF 199
Query: 220 VQFIGKVAPIRSKGYNKQLVSHDTKSNNYNYRYTFSVEISPICREDLICLPHHVALSLG- 278
IG + P++S N ++ T+ RY FSVEI PIC DLI LP +V SLG
Sbjct: 200 ATLIGDIVPVKS---NWAMIIPRTE------RYLFSVEIVPICCGDLIFLPPNVVASLGP 352
Query: 279 NLGPLVICTKVTNSI-----VLLDP 298
N+GP+VICT+ +I + LDP
Sbjct: 353 NIGPIVICTRGCQNIYFA*SIYLDP 427
Score = 31.6 bits (70), Expect(2) = 8e-17
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +1
Query: 303 HCFLDASQYWRASF 316
HCFLDA QYW+A F
Sbjct: 427 HCFLDAHQYWKAPF 468
>BG447717
Length = 407
Score = 42.4 bits (98), Expect = 4e-04
Identities = 28/77 (36%), Positives = 43/77 (55%), Gaps = 4/77 (5%)
Frame = +3
Query: 156 RVAANPDQWVAVVQLRQ-HVSHRRTFFYLEQL-ILKH-GAAARAIRIK-QMHEGIDFYFS 211
+V+ + W AVV +RQ HVS RT F+LEQ+ + H G RI+ ++EG+ FYF+
Sbjct: 147 KVSKMEEGWRAVVNVRQQHVSELRTLFHLEQVSVWNHLGIVGNFRRIEYNINEGLSFYFN 326
Query: 212 NEDHGEKFVQFIGKVAP 228
+ + V I + P
Sbjct: 327 SLSDAAEVVNLIXDILP 377
>BF635945
Length = 296
Score = 32.3 bits (72), Expect = 0.39
Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
Frame = +1
Query: 164 WVAVVQLRQ-HVSHRRTFFYLEQLILKHGAA--ARAIRIKQM---HEGIDFYFSNEDHGE 217
WV V+ +RQ HV RT F+LEQ+ + + +RIK EG+ F F++
Sbjct: 61 WVGVLNVRQQHVCQLRTLFHLEQISVWTNLTMLSHIMRIKNKIDDDEGLSFCFNSLSDAT 240
Query: 218 KFVQFIGKVAP 228
V I + P
Sbjct: 241 DVVNLIVDILP 273
>TC85803 similar to GP|13785471|dbj|BAB43909. phosphoenolpyruvate
carboxykinase {Flaveria pringlei}, partial (95%)
Length = 2351
Score = 30.0 bits (66), Expect = 1.9
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +2
Query: 418 YVEKRLKKRGKPRSWKLKSLNMEIDDKGRTDPDKMSSEYEL 458
+ EK+LKK G + WK K N E + + DK E +L
Sbjct: 113 FFEKKLKKNGIKQKWKWKYCNREREQWASKNYDKEKCERDL 235
>TC85752 similar to GP|21706788|gb|AAH34388.1 Unknown (protein for
MGC:34661) {Homo sapiens}, partial (80%)
Length = 827
Score = 30.0 bits (66), Expect = 1.9
Identities = 18/70 (25%), Positives = 31/70 (43%), Gaps = 4/70 (5%)
Frame = -3
Query: 28 CMCGIPMQPNAANMCVKCLSSQEDITEGLQKHINIFHCPQCESYFQHPKTWI----KHLK 83
C G+P+ + C K L +++ + Q H + H CE QHP+ + + K
Sbjct: 408 CNLGMPLNEYCMHHCQKSLKLVQELLQ--QPH*LLLHVEWCEDPQQHPRPCVPLHFQFQK 235
Query: 84 PESDELLKFC 93
P ++ L C
Sbjct: 234 PYLEQWLNQC 205
>TC78790 similar to PIR|T45678|T45678 hypothetical protein F14P22.170 -
Arabidopsis thaliana, partial (69%)
Length = 1681
Score = 29.3 bits (64), Expect = 3.3
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +1
Query: 3 GSSGICMEIEAGMFSVPQTICSVLCCMCGIPMQPNAANMCV 43
G S +C+ + P IC VLC +C I NA +C+
Sbjct: 1453 GRSCLCLFLRYLHVGFPAMICFVLCHLCFIEKNDNALLICL 1575
>TC76645 similar to PIR|S16294|S16294 chlorophyll a/b-binding protein type I
precursor - tomato, partial (89%)
Length = 1071
Score = 28.5 bits (62), Expect = 5.7
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +3
Query: 381 PGDYALGYDLYGANCNDIELDKYKGGDLPHA 411
PGDY GYD +G + + KY+G +L HA
Sbjct: 375 PGDY--GYDPFGLSKKPEDFAKYQGYELIHA 461
>CA919710 similar to GP|10177798|dbj emb|CAB87778.1~gene_id:MXA21.9~strong
similarity to unknown protein {Arabidopsis thaliana},
partial (18%)
Length = 738
Score = 27.7 bits (60), Expect = 9.7
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = -1
Query: 203 HEGIDFYFSNEDHGEKFVQFIGKVAPIRSKGYNKQLVSHDTKSNN 247
H ++ Y H +KFVQ +GK P + G K+ V +N
Sbjct: 693 HCNLELYPEFIKHLKKFVQTLGKPKPTTANGSEKEAVVETENQSN 559
>BQ151358 similar to GP|11993889|gb| virion-associated nuclear-shuttling
protein {Mus musculus}, partial (3%)
Length = 1195
Score = 27.7 bits (60), Expect = 9.7
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = +1
Query: 240 SHDTKSNNYNYRYTFSVEISPICREDL 266
+++T S+N YRYT ++ SP+ R+ +
Sbjct: 802 TYNTPSHNPQYRYTHNILCSPLVRDSI 882
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.137 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,250,241
Number of Sequences: 36976
Number of extensions: 256436
Number of successful extensions: 1331
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1318
length of query: 458
length of database: 9,014,727
effective HSP length: 99
effective length of query: 359
effective length of database: 5,354,103
effective search space: 1922122977
effective search space used: 1922122977
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0106.9