BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.9
(705 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG644741 54 2e-07
BE997611 40 0.003
BG586274 38 0.012
AW689408 33 0.37
CB065512 similar to GP|3608154|gb| unknown protein {Arabidopsis ... 31 1.4
BM814961 similar to GP|7298034|gb| CG6108 gene product {Drosophi... 30 3.2
TC91311 similar to GP|1684845|gb|AAB48303.1| pinin {Canis famili... 30 3.2
BE325816 weakly similar to GP|15028133|gb| unknown protein {Arab... 30 3.2
TC93153 similar to GP|14715220|emb|CAC44106. gag polyprotein {Ci... 30 3.2
TC86908 similar to PIR|G96708|G96708 hypothetical protein T26J14... 30 4.1
TC86693 similar to SP|Q9SP35|IM17_ARATH Mitochondrial import inn... 29 5.4
TC77685 apyrase-like protein [Medicago truncatula] 29 5.4
TC90578 homologue to GP|10178144|dbj|BAB11589. gene_id:MPO12.6~u... 29 7.1
TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 28 9.2
>BG644741
Length = 735
Score = 54.3 bits (129), Expect = 2e-07
Identities = 36/136 (26%), Positives = 60/136 (43%), Gaps = 1/136 (0%)
Frame = -2
Query: 60 MPM-LT*SGSSETAVLHVFPNAELVRLQLFPFSLRDTAEEWLNSQPQGSITTWEDLAKKF 118
MP+ L * ++ V + + ++ L++FP SL A+ W P SI TW L F
Sbjct: 665 MPI*LR*GAVCKSCVGRPYLDLNVLGLRVFPLSLMGEADIWFTELPYNSIFTWNQLRDVF 486
Query: 119 TKKLFPRTLLRKLKNDILVFKQEDTENLHEALERFKKLLRKCPQHNLTLGVQVERFYDGL 178
+ +P + + + F E++ + +RF LR P H + E FY G
Sbjct: 485 LARYYPVSKKLNHNDRVNNFVALPGESVSSSWDRFTSFLRSVPNHRIDDDSLKEYFYRGQ 306
Query: 179 ADSARSNLEAAASGEF 194
D+ + L+ A G +
Sbjct: 305 DDNNKVVLDTIAGGSY 258
>BE997611
Length = 547
Score = 40.0 bits (92), Expect = 0.003
Identities = 32/115 (27%), Positives = 54/115 (46%), Gaps = 1/115 (0%)
Frame = -2
Query: 427 KKKQKEKEEEKRKVEL-ENKFTKVLFPPFPTNIAKRRLEKQFSKFISMFKKLRVELPFSE 485
K KQ ++E VE+ E+K P ++ + +F+ + K +V +P +E
Sbjct: 528 KSKQNMEDEHINAVEVAEDK------PQTEDA*EEKEVPPKFNALHHILKGYKVNMPITE 367
Query: 486 VLEKMPQYAKFMKEILSKKRRLSEENEIIELTEECSAILQRKLPPKRKDPGSFTL 540
+E++P KF++E+L LSEE E I L+ E + + G FTL
Sbjct: 366 TVEQIPCCIKFLQELLKTNANLSEE-EFISLSSEFHHTYEVPAVVRFDGEGCFTL 205
>BG586274
Length = 546
Score = 38.1 bits (87), Expect = 0.012
Identities = 38/181 (20%), Positives = 71/181 (38%)
Frame = -3
Query: 241 QLSQQMTTIQRQFQAAKISNVDSIHCGTCGGPHASEECGTYFDEEVKVLGNSQNNPYSNT 300
Q +++T +Q + + G P S++ T EV+ L + + N
Sbjct: 538 QPRKKLTALQDKIDILRADKATQEQLHFVGNP--SQDTPTVV-HEVEGLEGQEELCFINN 368
Query: 301 YNLGWRNHPNFSWREQNNFNQGGNNQRQYSNQRFQSQNSGPQQNQDQGSGNGKKSLEELM 360
++ PNF + + N+Q Y + Q + PQQN GS ++S + +
Sbjct: 367 NGSWYKKEPNFQYNNYQQKSYPNNHQSGYPPRNNQQGSYQPQQNPSSGSSAPQESSTDTL 188
Query: 361 ENFINKADTSFKNHEAAIKSLETQVGQMAKQMSERPPGMFPSDTVINPKENCSAITLRNI 420
I ++ T + H VG K + + G + +N K + A T++N+
Sbjct: 187 LKQILESQTRSEKH----------VGYELKNLHSKIDGSY---NELNNKFSHLASTVKNL 47
Query: 421 E 421
E
Sbjct: 46 E 44
>AW689408
Length = 583
Score = 33.1 bits (74), Expect = 0.37
Identities = 21/68 (30%), Positives = 32/68 (46%), Gaps = 7/68 (10%)
Frame = +2
Query: 292 SQNNPYSNTYNLGWRNHPNFSWREQNNF-------NQGGNNQRQYSNQRFQSQNSGPQQN 344
S+ +P +N +N +H FS++ NN+ NQ N Q+SN Q+ N P Q+
Sbjct: 179 SEMDPNNNQFNTQNSSHYTFSYQNPNNYQHPNQFPNQHLLNPNQFSN*HPQNSNQFPNQH 358
Query: 345 QDQGSGNG 352
S G
Sbjct: 359 PQNMSNFG 382
>CB065512 similar to GP|3608154|gb| unknown protein {Arabidopsis thaliana},
partial (34%)
Length = 852
Score = 31.2 bits (69), Expect = 1.4
Identities = 31/110 (28%), Positives = 53/110 (48%), Gaps = 6/110 (5%)
Frame = +1
Query: 416 TLRNIEPLGENKKKQKEKEEE---KRKVELENKFTKVLFPPFPTNIAKRRLEKQFSKFIS 472
TLR + KK K+ E+ KR E+EN K N A RRLE + ++ +
Sbjct: 46 TLRMDREETQKLKKGKQALEDTTMKRLSEMENALRKASGQVDRANGAVRRLETENAEIRA 225
Query: 473 MFK--KLRVELPFSEVLEKMPQYAKFMKEILS-KKRRLSEENEIIELTEE 519
+ KL + LE + K++K++L+ +K++ + EI +L E+
Sbjct: 226 EMEASKLSASESVTACLEVAKKEKKYLKKLLAWEKQKAKLQKEISDLKEK 375
>BM814961 similar to GP|7298034|gb| CG6108 gene product {Drosophila
melanogaster}, partial (1%)
Length = 744
Score = 30.0 bits (66), Expect = 3.2
Identities = 27/88 (30%), Positives = 40/88 (44%), Gaps = 1/88 (1%)
Frame = +2
Query: 425 ENKKKQKEKEEEKRK-VELENKFTKVLFPPFPTNIAKRRLEKQFSKFISMFKKLRVELPF 483
E KKKQKE+EE K K ELE L+PP +K + +
Sbjct: 32 EEKKKQKEEEEAKEKEKELE------LYPP--------------------EEKDKPDPQL 133
Query: 484 SEVLEKMPQYAKFMKEILSKKRRLSEEN 511
SE+ E++ + + +KEI K++ S N
Sbjct: 134 SEMKERLEKLEETVKEIAVVKKKQSSSN 217
>TC91311 similar to GP|1684845|gb|AAB48303.1| pinin {Canis familiaris},
partial (3%)
Length = 746
Score = 30.0 bits (66), Expect = 3.2
Identities = 16/75 (21%), Positives = 34/75 (45%)
Frame = -1
Query: 227 LEIEAYDRMVASNKQLSQQMTTIQRQFQAAKISNVDSIHCGTCGGPHASEECGTYFDEEV 286
LEI R++ + K + +M+ ++ + + CG+CGG +E C Y + +
Sbjct: 374 LEISTVPRLLNAMKMIMMKMSRKMKKRGKIRY*WFTNQGCGSCGGCWVTEYCHHYIECFI 195
Query: 287 KVLGNSQNNPYSNTY 301
+ N + ++Y
Sbjct: 194 AAIDNKNESDGGSSY 150
>BE325816 weakly similar to GP|15028133|gb| unknown protein {Arabidopsis
thaliana}, partial (7%)
Length = 673
Score = 30.0 bits (66), Expect = 3.2
Identities = 15/38 (39%), Positives = 21/38 (54%)
Frame = +3
Query: 302 NLGWRNHPNFSWREQNNFNQGGNNQRQYSNQRFQSQNS 339
NLG R PN +QNN GGNN+ + +Q + + S
Sbjct: 93 NLGKRTEPN----QQNNAKSGGNNEERTHHQEERGRKS 194
>TC93153 similar to GP|14715220|emb|CAC44106. gag polyprotein {Cicer
arietinum}, partial (8%)
Length = 516
Score = 30.0 bits (66), Expect = 3.2
Identities = 22/97 (22%), Positives = 42/97 (42%), Gaps = 5/97 (5%)
Frame = +2
Query: 73 VLHVFPNAELVRLQLFPFSLRDTAEEWLNS-----QPQGSITTWEDLAKKFTKKLFPRTL 127
+ V E ++Q L + A++W S + ++ TW K+F + FP +
Sbjct: 197 IFRVMQCFETQKVQFGTHMLAEEADDWWISLLPVLEQDDAVVTWAMFRKEFLGRYFPEDV 376
Query: 128 LRKLKNDILVFKQEDTENLHEALERFKKLLRKCPQHN 164
K + + L KQ D ++ E +F +L P ++
Sbjct: 377 RGKKEIEFLELKQGDM-SVTEYAAKFVELATFYPHYS 484
>TC86908 similar to PIR|G96708|G96708 hypothetical protein T26J14.4
[imported] - Arabidopsis thaliana, partial (65%)
Length = 1587
Score = 29.6 bits (65), Expect = 4.1
Identities = 16/56 (28%), Positives = 31/56 (54%), Gaps = 3/56 (5%)
Frame = +1
Query: 425 ENKKKQKEKEEEKRKVELENKFTKVLFPP---FPTNIAKRRLEKQFSKFISMFKKL 477
+ KKK+K+K+++K+ L + + PP F N+ ++ + SKF +M K+
Sbjct: 88 KKKKKKKKKKKKKKTSRLVPFISYISLPPSLFFLLNVFQKVITNSTSKFSTMHNKI 255
>TC86693 similar to SP|Q9SP35|IM17_ARATH Mitochondrial import inner membrane
translocase subunit TIM17. [Mouse-ear cress], partial
(62%)
Length = 1047
Score = 29.3 bits (64), Expect = 5.4
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -3
Query: 318 NFNQGGNNQRQYSNQRFQSQNSGPQQN 344
N NQG N +SN FQ+ NS PQ +
Sbjct: 784 NINQGSNQTLIFSNLNFQNTNSLPQNS 704
>TC77685 apyrase-like protein [Medicago truncatula]
Length = 2172
Score = 29.3 bits (64), Expect = 5.4
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 4/94 (4%)
Frame = +1
Query: 522 AILQRKLPPKRKDPGSFTLPVNFGASKQVRALCDLGSSVNLM----PLSMFERLNVGELK 577
A+L RKL P +K GS+ + + G+S + +++L+ L +FE+L G L
Sbjct: 499 ALLNRKLSPDKKSGGSYAVIFDAGSSGSRVHVFHFDQNLDLVHIGKDLELFEQLKPG-LS 675
Query: 578 PTMMMLQLADRSIVAPWGVVEDVLVRVGEFEFPV 611
Q A S+V+ E V+ R + PV
Sbjct: 676 AYAQKPQQAAESLVSLLEKAEGVVPRELRSKTPV 777
>TC90578 homologue to GP|10178144|dbj|BAB11589. gene_id:MPO12.6~unknown
protein {Arabidopsis thaliana}, partial (1%)
Length = 1100
Score = 28.9 bits (63), Expect = 7.1
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +2
Query: 424 GENKKKQKEKEEEKRKVELENKFTKVLFPPFPTNI 458
GEN + + ++ EK + E K +K L PPF T+I
Sbjct: 14 GENDEGKPKEPNEKGFLSRERKKSKYLSPPFTTSI 118
>TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (13%)
Length = 663
Score = 28.5 bits (62), Expect = 9.2
Identities = 23/125 (18%), Positives = 50/125 (39%)
Frame = +3
Query: 321 QGGNNQRQYSNQRFQSQNSGPQQNQDQGSGNGKKSLEELMENFINKADTSFKNHEAAIKS 380
+ G+ ++ ++ + + G + G K +E ++ K + + + + K
Sbjct: 303 EDGDEKKDKEKKKKEKKEKGKEDKDKDGEEKKSKKDKEKKKD---KNEDDDEGEDGSKKK 473
Query: 381 LETQVGQMAKQMSERPPGMFPSDTVINPKENCSAITLRNIEPLGENKKKQKEKEEEKRKV 440
+ K+ E+ G K + I + G+ KKK+KE +EEK+K
Sbjct: 474 KNKDKKEKKKEEDEKEEG----------KVSVRDIDIEETAKEGKEKKKKKEDKEEKKKK 623
Query: 441 ELENK 445
++K
Sbjct: 624 SGKDK 638
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.135 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,156,875
Number of Sequences: 36976
Number of extensions: 228864
Number of successful extensions: 1384
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1381
length of query: 705
length of database: 9,014,727
effective HSP length: 103
effective length of query: 602
effective length of database: 5,206,199
effective search space: 3134131798
effective search space used: 3134131798
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0103.9