BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.4
(378 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93234 weakly similar to GP|21751020|dbj|BAC03887. unnamed prot... 34 0.081
BQ751207 weakly similar to GP|604427|gb|AA ACOB protein {Emerice... 34 0.11
TC79633 weakly similar to GP|15450978|gb|AAK96760.1 putative pro... 33 0.18
BQ143841 32 0.40
TC84327 31 0.69
TC86158 homologue to SP|P49608|ACOC_CUCMA Aconitate hydratase c... 31 0.69
TC85577 homologue to GP|6715512|gb|AAF26445.1| vacuolar H+-ATPas... 31 0.69
TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean... 31 0.69
BF635325 similar to GP|4204575|gb|A cytochrome b5 DIF-F {Petunia... 31 0.90
BE322935 similar to GP|18073605|emb hypothetical protein {Grapev... 31 0.90
TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog... 30 1.2
BQ150687 30 1.5
TC88504 similar to GP|13442983|gb|AAK02067.1 cyclophilin-40 {Ara... 30 1.5
TC86898 similar to GP|18376007|emb|CAB91741. probable ATP citrat... 30 2.0
BG582083 29 2.6
TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like... 29 2.6
TC77148 ENOD20 29 3.4
BQ751226 similar to GP|18376350|em probable aldehyde dehydrogena... 29 3.4
AW586147 similar to PIR|E96772|E96 hypothetical protein F1M20.6 ... 28 4.4
BQ152604 similar to GP|22759723|dbj| protease inhibitor 2 {Zinni... 28 4.4
>TC93234 weakly similar to GP|21751020|dbj|BAC03887. unnamed protein product
{Homo sapiens}, partial (11%)
Length = 591
Score = 34.3 bits (77), Expect = 0.081
Identities = 27/86 (31%), Positives = 37/86 (42%), Gaps = 3/86 (3%)
Frame = +2
Query: 223 TGVRPPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPR---PHPALASPS 279
TG PP + + S + P + SP T + TP+T PP P P + P+
Sbjct: 251 TGTAPPFAVSSPASSP--TTPSSTRSSPGSTSTPRTPSTSFSARPPPSTPPPTPFRSRPT 424
Query: 280 LASSTLADPRSGPSVTQSILPTVPAM 305
SS P S PS + PT PA+
Sbjct: 425 KGSS----PSSTPSSSSPPAPTSPAI 490
>BQ751207 weakly similar to GP|604427|gb|AA ACOB protein {Emericella
nidulans}, partial (12%)
Length = 758
Score = 33.9 bits (76), Expect = 0.11
Identities = 25/94 (26%), Positives = 41/94 (43%), Gaps = 8/94 (8%)
Frame = +1
Query: 237 SRLGSNPMNKLDSPPKTDSIITPNTRLK-------IHVPPRPHPALASPSLASSTLADPR 289
S+ S P+ SPP T + +P++ I PP P PA+ SPS +
Sbjct: 160 SQNSSKPLRSRPSPPPTFTPTSPSSSFSLTAHTPTISPPPPPPPAITSPSSTPPRPSSSA 339
Query: 290 SGPS-VTQSILPTVPAMENCKIAMHRNPPKSQVN 322
+ PS ++ + PT P + +R P S+ +
Sbjct: 340 NSPS*LSLTPAPTSPTPPYSPPSTYRRPAFSRTS 441
>TC79633 weakly similar to GP|15450978|gb|AAK96760.1 putative protein
{Arabidopsis thaliana}, partial (78%)
Length = 1054
Score = 33.1 bits (74), Expect = 0.18
Identities = 24/63 (38%), Positives = 32/63 (50%), Gaps = 4/63 (6%)
Frame = +3
Query: 244 MNKLDSPPKTDSIITPNTRLKIHVPPRPHPALA--SP--SLASSTLADPRSGPSVTQSIL 299
+N PP + TP T + P P +L+ SP SL+SS+ A GPS T S+L
Sbjct: 303 LNPSPMPPSVSAKTTP-TSVSTTTPSLPESSLSLSSPIHSLSSSSSASSLHGPSSTSSVL 479
Query: 300 PTV 302
P V
Sbjct: 480 PIV 488
>BQ143841
Length = 562
Score = 32.0 bits (71), Expect = 0.40
Identities = 25/76 (32%), Positives = 39/76 (50%)
Frame = +1
Query: 17 IINCLLSPNRHIPSGTVYLYTFGYSIYLRVQYIYIPSGTVYTFGYSISIYLRVQYIPSGT 76
I + + S NR+I +Y Y YS ++ Q+ TVYT + IY+ +Y+
Sbjct: 211 ICSVIDSDNRYILR-YIYKYYHKYSTFILYQH------TVYT---NS*IYILYKYLFVRP 360
Query: 77 VYLYTFGYNIYLRVQY 92
++LYT YNIY + Y
Sbjct: 361 IHLYTLYYNIYSILLY 408
Score = 30.4 bits (67), Expect = 1.2
Identities = 30/112 (26%), Positives = 50/112 (43%), Gaps = 4/112 (3%)
Frame = +1
Query: 27 HIPSGTVYLYTFGYSIYLRVQYIYIPSGTVYTFGYSISIYLRV-QYIPSGTVYLYTFGYN 85
H +G + T + Y+ + Y+ S T Y I I+ + I S Y+ + Y
Sbjct: 85 HP*NGVMVFCTRFFHFYILIIYLV*FSLTSLYDMYIIYIHSIICSVIDSDNRYILRYIYK 264
Query: 86 IYLRVQYFYIPSGTVYTFGYSISIYLRVQYIPSGTVYLYTFGY---SISIYL 134
Y + F + TVYT + IY+ +Y+ ++LYT Y SI +Y+
Sbjct: 265 YYHKYSTFILYQHTVYT---NS*IYILYKYLFVRPIHLYTLYYNIYSILLYM 411
>TC84327
Length = 847
Score = 31.2 bits (69), Expect = 0.69
Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = -1
Query: 13 RTPFIINCLLSPNRHIPS--GTVYLYTFGYSIYLRVQYIY 50
R FII +++ +HI VYLY + YSIY QY+Y
Sbjct: 211 RNYFIIIKIVNIKKHI*Q*LADVYLYVYYYSIYYYYQYVY 92
>TC86158 homologue to SP|P49608|ACOC_CUCMA Aconitate hydratase cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)., complete
Length = 3280
Score = 31.2 bits (69), Expect = 0.69
Identities = 20/67 (29%), Positives = 29/67 (42%), Gaps = 5/67 (7%)
Frame = -3
Query: 249 SPPKTDSIITPNTRLKIHVPPRPHPALASPSL-----ASSTLADPRSGPSVTQSILPTVP 303
+P T I P+ +PP PHPA SPS+ + T S PS+ ++ P
Sbjct: 1022 TPGNTMLIGWPSIAASASIPPTPHPATPSPSIIVVWESVPTTLSG*SNPSLLKTTRPRYS 843
Query: 304 AMENCKI 310
+ C I
Sbjct: 842 RLTWCTI 822
>TC85577 homologue to GP|6715512|gb|AAF26445.1| vacuolar H+-ATPase B subunit
{Nicotiana tabacum}, partial (14%)
Length = 841
Score = 31.2 bits (69), Expect = 0.69
Identities = 19/64 (29%), Positives = 32/64 (49%)
Frame = -1
Query: 241 SNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSILP 300
S ++ + +PPK+ +P+ + PP P SPS +S+ P P+VT S +
Sbjct: 469 SPTISPVATPPKSSQAPSPSAVSPVASPPVPVKNAPSPSPPASS-DSPAVSPAVTPSSIS 293
Query: 301 TVPA 304
T P+
Sbjct: 292 TTPS 281
>TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean, partial
(93%)
Length = 1554
Score = 31.2 bits (69), Expect = 0.69
Identities = 28/110 (25%), Positives = 44/110 (39%), Gaps = 19/110 (17%)
Frame = -2
Query: 228 PISQHNKKVSRLGSNPMNKLDSPPKTDSIITPN-----------TRLKIHVPPRPHPALA 276
P++ K++ S+P PPK+ + TP + + PP P P A
Sbjct: 713 PVTSPAPKIAP-ASSPKVPPPQPPKSSPVSTPTLPPPLPPPPKISPTPVQTPPAPAPVKA 537
Query: 277 SPSLASSTLAD-----PRSGPSV---TQSILPTVPAMENCKIAMHRNPPK 318
+P A + P + P + T +I VPA E+ K R+ PK
Sbjct: 536 TPVPAPAPAKQAPTPAPATSPPIPAPTPAIEAPVPAPESSKPKRRRHRPK 387
>BF635325 similar to GP|4204575|gb|A cytochrome b5 DIF-F {Petunia x hybrida},
partial (35%)
Length = 682
Score = 30.8 bits (68), Expect = 0.90
Identities = 20/49 (40%), Positives = 27/49 (54%), Gaps = 2/49 (4%)
Frame = -1
Query: 247 LDSPPKTDSIITPNTRLKIH--VPPRPHPALASPSLASSTLADPRSGPS 293
LD PP+T S TP R K +PP+PHP +P A++T +G S
Sbjct: 196 LDVPPRTLSHPTPAPRPKKFNGIPPKPHP---TPKPATNTETKSDAGTS 59
>BE322935 similar to GP|18073605|emb hypothetical protein {Grapevine fleck
virus}, partial (21%)
Length = 698
Score = 30.8 bits (68), Expect = 0.90
Identities = 23/83 (27%), Positives = 35/83 (41%), Gaps = 8/83 (9%)
Frame = +2
Query: 228 PISQHNKKVSRLGSNPMNKLDSPPKTDSI-ITPNTRLKIHVPPRPHPALAS-------PS 279
P S K S ++P+N + S P ++ K H PP HP + S PS
Sbjct: 71 PPSAPPSKPSYTLTHPINPVTSSPSLSQP*SATSSASKTHAPPHLHPTVGSTSPSFTNPS 250
Query: 280 LASSTLADPRSGPSVTQSILPTV 302
SS +P PS ++ P++
Sbjct: 251 SLSSPPPEPSPPPSPPSTVSPSL 319
>TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog -
cucumber, partial (49%)
Length = 1486
Score = 30.4 bits (67), Expect = 1.2
Identities = 31/99 (31%), Positives = 42/99 (42%), Gaps = 3/99 (3%)
Frame = +3
Query: 250 PPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSILPTVPAM---E 306
PPK + L PP HPA SPS P S LPT A+
Sbjct: 21 PPKPAAKTCQQPPLPPPTPPHHHPAPPSPS---------HPVPPSKHSSLPTPSALVL*H 173
Query: 307 NCKIAMHRNPPKSQVNYTTILQGNLLVNQSNHLLPATNN 345
+ ++ NPP S +++ +L +LL+ S HLLP N
Sbjct: 174 LFQASLQLNPPPS-LSFLLVLWLHLLL--SLHLLPLLVN 281
>BQ150687
Length = 445
Score = 30.0 bits (66), Expect = 1.5
Identities = 25/101 (24%), Positives = 42/101 (40%), Gaps = 3/101 (2%)
Frame = +2
Query: 29 PSGTVYLYTFGYSIYLRVQYIYIPSGTVYTFGYSISIYLRVQYIPSGTVYLYTFGYNIYL 88
P+ +++Y + IY+R YIYI T + I L +Q + V + Y Y+
Sbjct: 122 PNFHIHIYFYYNHIYMRTMYIYILHRYNNTVNLTTHI*LYIQTLLIHYVDVLIPPYTFYI 301
Query: 89 RVQYFYIPSGTVYTFGYS---ISIYLRVQYIPSGTVYLYTF 126
PS +Y + S I +Y+ Q+ T+ F
Sbjct: 302 FSFAIVSPSPFLYNYN*SR*LIQLYIPNQHFTIPTIIFILF 424
>TC88504 similar to GP|13442983|gb|AAK02067.1 cyclophilin-40 {Arabidopsis
thaliana}, partial (94%)
Length = 1460
Score = 30.0 bits (66), Expect = 1.5
Identities = 21/77 (27%), Positives = 34/77 (43%), Gaps = 3/77 (3%)
Frame = +3
Query: 241 SNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLA---SSTLADPRSGPSVTQS 297
S+PM+ PPK + P + +H P SPS++ SST++ S S
Sbjct: 198 SSPMS-FPKPPKISDLSAPAKKASVHTP-------TSPSISRTPSSTVSSKVS*SKAVTS 353
Query: 298 ILPTVPAMENCKIAMHR 314
+ PT PA + + +
Sbjct: 354 LPPTEPAASQSTVQISK 404
>TC86898 similar to GP|18376007|emb|CAB91741. probable ATP citrate lyase
subunit 2 {Neurospora crassa}, complete
Length = 2389
Score = 29.6 bits (65), Expect = 2.0
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = +3
Query: 249 SPPKTDSIITPNTRLKIHVPPRPHPA---LASPSLASSTLADPRSGPSVTQSILPTVP 303
SPP +P T + +PP P P + S + S+ L R+ S++ +LPT P
Sbjct: 1614 SPPPASPTSSPTTVSTLVLPPSPRPTTTPVLSSTSCSALL*PRRARSSLSVVVLPTSP 1787
>BG582083
Length = 665
Score = 29.3 bits (64), Expect = 2.6
Identities = 25/95 (26%), Positives = 45/95 (47%)
Frame = -3
Query: 15 PFIINCLLSPNRHIPSGTVYLYTFGYSIYLRVQYIYIPSGTVYTFGYSISIYLRVQYIPS 74
P ++ L SPN I + T Y+Y S Y+ +Q +P + G +Y RV++
Sbjct: 477 PDVVYIL*SPNNSIINRTYYIYK--PSAYVNLQAPILP----FLLGV---LYFRVKHFTG 325
Query: 75 GTVYLYTFGYNIYLRVQYFYIPSGTVYTFGYSISI 109
+ + + + I+ R++ +P YT+G SI
Sbjct: 324 HSNIIKGWIFEIFHRLKRITLPK---YTYGEPASI 229
>TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like protein
{Arabidopsis thaliana}, partial (16%)
Length = 814
Score = 29.3 bits (64), Expect = 2.6
Identities = 23/64 (35%), Positives = 27/64 (41%), Gaps = 2/64 (3%)
Frame = +3
Query: 241 SNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALA--SPSLASSTLADPRSGPSVTQSI 298
S P N SPP T +I P+ PP P + SPS SS P S PS +
Sbjct: 150 STPSNSPPSPPTTPAISPPSGGGTTPSPPSRTPPSSDDSPSPPSSKTPPPPSPPSSSSIS 329
Query: 299 LPTV 302
TV
Sbjct: 330 TGTV 341
>TC77148 ENOD20
Length = 1108
Score = 28.9 bits (63), Expect = 3.4
Identities = 25/81 (30%), Positives = 35/81 (42%), Gaps = 7/81 (8%)
Frame = +3
Query: 247 LDSPPKTDSIITPNTRLKIHVPPR---PHPALASPSLASSTLADPRSGPSVTQSILP--- 300
L SPP S TP + I PPR P P SPS + S P PS + +P
Sbjct: 417 LSSPPPPPSPPTPRSSTPIPHPPRRSLPSPPSPSPSPSPS----PSPSPSPRSTPIPHPR 584
Query: 301 -TVPAMENCKIAMHRNPPKSQ 320
PA + ++ ++P S+
Sbjct: 585 KRSPASPSPSPSLSKSPSPSE 647
>BQ751226 similar to GP|18376350|em probable aldehyde dehydrogenase
{Neurospora crassa}, partial (39%)
Length = 807
Score = 28.9 bits (63), Expect = 3.4
Identities = 22/63 (34%), Positives = 27/63 (41%)
Frame = +2
Query: 241 SNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSILP 300
S P+ PP+ + P+TR + P P A PS AS T S PS S P
Sbjct: 194 SRPLRSSTPPPRRS--LPPSTRPPRRMSTSPSPPPARPSRASGTRPPLTSAPSCC*S-SP 364
Query: 301 TVP 303
T P
Sbjct: 365 TSP 373
>AW586147 similar to PIR|E96772|E96 hypothetical protein F1M20.6 [imported] -
Arabidopsis thaliana, partial (28%)
Length = 534
Score = 28.5 bits (62), Expect = 4.4
Identities = 27/104 (25%), Positives = 43/104 (40%), Gaps = 3/104 (2%)
Frame = +1
Query: 229 ISQHN---KKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTL 285
IS+H+ K S + S+P + +P S PP P P + S T
Sbjct: 133 ISKHSTTLKSPSSVASSPSSSYVAP*ALTSF-----------PPTPTPLTKT*S-KKPTA 276
Query: 286 ADPRSGPSVTQSILPTVPAMENCKIAMHRNPPKSQVNYTTILQG 329
+ PRS P+ T IL + ++ + + PKS + T+ G
Sbjct: 277 SSPRSDPTPTHLILTIKTTPFSTQMPLLPSAPKSPIGILTVKLG 408
>BQ152604 similar to GP|22759723|dbj| protease inhibitor 2 {Zinnia elegans},
partial (74%)
Length = 477
Score = 28.5 bits (62), Expect = 4.4
Identities = 24/67 (35%), Positives = 36/67 (52%), Gaps = 11/67 (16%)
Frame = -3
Query: 77 VYLYTFGYNIYLRVQY---FYIPSGTVYTFGYSISIYL--------RVQYIPSGTVYLYT 125
+Y+Y + Y IY+ VQ+ FY GT+ T S+S R+ PSGT+ +T
Sbjct: 352 IYIYIYIY-IYI*VQFHDTFYPIVGTLKTIP-SLSTQTQTLSQRKSRMTADPSGTMMAFT 179
Query: 126 FGYSISI 132
G+S+SI
Sbjct: 178 NGFSLSI 158
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,400,734
Number of Sequences: 36976
Number of extensions: 210227
Number of successful extensions: 1567
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 1348
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1552
length of query: 378
length of database: 9,014,727
effective HSP length: 98
effective length of query: 280
effective length of database: 5,391,079
effective search space: 1509502120
effective search space used: 1509502120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0103.4