BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.3
(186 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ151224 31 0.25
TC90465 similar to GP|12710364|emb|CAC28452. unnamed protein pro... 29 0.94
TC84639 similar to GP|20466750|gb|AAM20692.1 unknown protein {Ar... 28 2.1
AW584186 27 3.6
TC90246 similar to PIR|T00872|T00872 probable protein kinase At2... 27 4.7
TC79586 similar to SP|P52425|GPDA_CUPLA Glycerol-3-phosphate deh... 27 6.1
TC88933 weakly similar to GP|17381142|gb|AAL36383.1 unknown prot... 26 8.0
TC79280 similar to GP|21592960|gb|AAM64910.1 unknown {Arabidopsi... 26 8.0
AW689071 26 8.0
TC90388 similar to PIR|B96837|B96837 hypothetical protein T21F11... 26 8.0
>BQ151224
Length = 405
Score = 31.2 bits (69), Expect = 0.25
Identities = 14/26 (53%), Positives = 16/26 (60%)
Frame = +1
Query: 161 LCKCKNIVICQNLMIILIANAECYKS 186
LC C V+C NLM I IA + CY S
Sbjct: 304 LCACSPRVLCLNLMNINIARSSCYYS 381
>TC90465 similar to GP|12710364|emb|CAC28452. unnamed protein product
{Glycine max}, partial (41%)
Length = 674
Score = 29.3 bits (64), Expect = 0.94
Identities = 21/79 (26%), Positives = 36/79 (44%)
Frame = -3
Query: 81 KSDQLNIGEWFLESWLVLDFEKECLGNKILEKDMKCENALVSELKLEGKRAGMRSEDVIG 140
+S+ L + WFL S + F CL +K+L + + S L G+R + +G
Sbjct: 288 RSNHLGVNFWFLASRFL--FSALCLSSKLLFSLPDTDRSPFSRAILSGRRRESDGNNFLG 115
Query: 141 SETQLRKEVAKWINMFYSF 159
+ ++L AK + SF
Sbjct: 114 AVSKLLSLCAK*VYWNLSF 58
>TC84639 similar to GP|20466750|gb|AAM20692.1 unknown protein {Arabidopsis
thaliana}, partial (54%)
Length = 799
Score = 28.1 bits (61), Expect = 2.1
Identities = 16/50 (32%), Positives = 21/50 (42%)
Frame = -2
Query: 49 ARWWIDPPCKCHGGIDSLVVPVNFSNSHFLNNKSDQLNIGEWFLESWLVL 98
A W P KCH ++P F F + S+ NI E F S +L
Sbjct: 447 AAWASKVP*KCHHSYSLFLIPKIFEQDRF-PSSSENTNIAEIFCNSGTIL 301
>AW584186
Length = 310
Score = 27.3 bits (59), Expect = 3.6
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 4/33 (12%)
Frame = -1
Query: 93 ESWLVLDFEKE----CLGNKILEKDMKCENALV 121
+SW+ F++ C G K+L KDM EN V
Sbjct: 214 KSWVAFGFKQSGYGYCKGEKLLVKDMHAENGRV 116
>TC90246 similar to PIR|T00872|T00872 probable protein kinase At2g45590
[imported] - Arabidopsis thaliana, partial (28%)
Length = 1451
Score = 26.9 bits (58), Expect = 4.7
Identities = 13/42 (30%), Positives = 18/42 (41%)
Frame = +2
Query: 13 DVAIKKNGLWWMGPTILYGDSTTYRKISMEDGAIVRARWWID 54
D I WWM LYGD ++ S + + WW+D
Sbjct: 464 DDGISNGDNWWMSDDALYGDRKKGKRRSGNNRS-GNIDWWLD 586
>TC79586 similar to SP|P52425|GPDA_CUPLA Glycerol-3-phosphate dehydrogenase
[NAD+] (EC 1.1.1.8). {Cuphea lanceolata}, partial (92%)
Length = 1521
Score = 26.6 bits (57), Expect = 6.1
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 152 WINMFYSFELCKCKNIVICQNLMII 176
W NM+ C+CK+ + CQ +MI+
Sbjct: 1358 WTNMY-----CQCKDFLYCQ*IMIV 1417
>TC88933 weakly similar to GP|17381142|gb|AAL36383.1 unknown protein
{Arabidopsis thaliana}, partial (73%)
Length = 1632
Score = 26.2 bits (56), Expect = 8.0
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = -3
Query: 55 PPCKCHGGIDSLVVPVNFSNSHFLNNK 81
PP G ID P + S SH+ N+K
Sbjct: 826 PPTPPTGYIDCTATPTSLSQSHYTNHK 746
>TC79280 similar to GP|21592960|gb|AAM64910.1 unknown {Arabidopsis
thaliana}, partial (46%)
Length = 809
Score = 26.2 bits (56), Expect = 8.0
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = +3
Query: 129 KRAGMRSEDVIGSETQLRKEVAKWINMFYSFELCK 163
K G +++ E Q ++E+ K++N+F + +LCK
Sbjct: 585 KHRGQEHNELVLEERQRKREI-KFVNIFLTEKLCK 686
>AW689071
Length = 585
Score = 26.2 bits (56), Expect = 8.0
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +2
Query: 158 SFELCKCKNIVICQNLMIILIANAEC 183
S+ LCKC++ + NLM+ + N C
Sbjct: 278 SYLLCKCRDKTLLNNLMVCNLFNNLC 355
>TC90388 similar to PIR|B96837|B96837 hypothetical protein T21F11.15
[imported] - Arabidopsis thaliana, partial (37%)
Length = 1208
Score = 26.2 bits (56), Expect = 8.0
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +2
Query: 102 KECLGNKILEKDMKCENALVSELKLEGKRAGMRSEDVIGSETQLRKEVAK 151
K+CLGN I+ K + CE + + + + RA S G+ + + V K
Sbjct: 248 KKCLGNYIMRKLVTCEESNSALVVVPTSRAS--SSSYYGTRSSWKNSVVK 391
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,029,189
Number of Sequences: 36976
Number of extensions: 106016
Number of successful extensions: 613
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 609
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 613
length of query: 186
length of database: 9,014,727
effective HSP length: 90
effective length of query: 96
effective length of database: 5,686,887
effective search space: 545941152
effective search space used: 545941152
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0103.3