BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.2
(681 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 35 0.12
TC80768 similar to GP|15081692|gb|AAK82501.1 At1g50380/F14I3_27 ... 34 0.21
BQ144307 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 33 0.36
TC78364 similar to GP|9759266|dbj|BAB09587.1 diacylglycerol kina... 33 0.47
BQ151301 similar to PIR|S34666|S346 glycine-rich protein - commo... 32 0.80
TC89118 weakly similar to PIR|T06098|T06098 hypothetical protein... 32 1.0
BQ751144 weakly similar to PIR|PQ0479|PQ04 pistil extensin-like ... 32 1.0
BQ141932 weakly similar to GP|6523547|emb hydroxyproline-rich gl... 32 1.0
TC87548 similar to PIR|T47553|T47553 peroxiredoxin-like protein ... 31 1.4
TC82997 homologue to GP|7293564|gb|AAF48937.1| CG7884 gene produ... 30 2.3
TC79709 similar to SP|Q39242|TRB2_ARATH Thioredoxin reductase 2 ... 30 2.3
TC91132 similar to GP|18087625|gb|AAL58942.1 AT4g22410/F7K2_7 {A... 30 2.3
BQ141543 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 30 2.3
BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, pa... 30 2.3
BG587776 weakly similar to PIR|F86242|F86 unknown protein 98896... 30 3.0
BF638182 weakly similar to PIR|T45594|T45 glucosidase-like prote... 30 4.0
TC79953 similar to GP|20279471|gb|AAM18751.1 unknown protein {Or... 30 4.0
TC91842 similar to GP|21427461|gb|AAM53243.1 actin-related prote... 30 4.0
BI272602 GP|11602812|db avenaIII {Gallus gallus}, partial (3%) 30 4.0
BE998536 similar to GP|2598593|emb| MtN12 {Medicago truncatula},... 29 5.2
>BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (16%)
Length = 993
Score = 34.7 bits (78), Expect = 0.12
Identities = 28/78 (35%), Positives = 33/78 (41%), Gaps = 5/78 (6%)
Frame = +3
Query: 470 PRPSPKRLNSSEPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSANAPPPPAHT----- 524
P PSP L+ PS P L+ L P PR P APPPPA
Sbjct: 228 PSPSPPPLSPPSPSPPPPPPQPLLGPGLRPP----GPRPP-------APPPPAPAPRPAP 374
Query: 525 STPSVGRISQLEKTLAEL 542
S PSVGR + +LA +
Sbjct: 375 SRPSVGRPRRAAPSLARI 428
>TC80768 similar to GP|15081692|gb|AAK82501.1 At1g50380/F14I3_27
{Arabidopsis thaliana}, partial (35%)
Length = 831
Score = 33.9 bits (76), Expect = 0.21
Identities = 38/180 (21%), Positives = 79/180 (43%), Gaps = 6/180 (3%)
Frame = +3
Query: 499 RPIVISSPRGPNLTSSANAPPPPAHTSTPSVGRISQLEKTLAELRQEAQLESSVKADQIA 558
RP+ S P+ +L+ + +P PP +++ + EL + ++++ +
Sbjct: 198 RPMTTSPPQSQSLSPHSQSPLPPP---------VAKKVEHKMELFNDVRIDNYYWLRDDS 350
Query: 559 STEAD-LENLRSDLAKKDDLLSSAKTQAHLDAKALEEKLRSEDAVAAVSERGRGFFL--- 614
++ D L LR + A D ++S K ++ +++ ED ++A +G ++
Sbjct: 351 RSDPDVLSYLRQENAYTDSVMSGTKKIEDKLFAEIKGRIKEED-ISAPVRKGPYYYYKKT 527
Query: 615 --AKAQVKHLHPLIDLSQMGAFKRVTPTGLVGPDDPPGFVVERFLTEEHVELTQNVIFVI 672
K V++ LI +Q V T GPD PP E + +E+++ Q+ + I
Sbjct: 528 LEGKEYVQYCRRLISDNQ--KVPSVNDTMPTGPDAPP----EHVILDENIKAQQHEYYSI 689
>BQ144307 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (24%)
Length = 1059
Score = 33.1 bits (74), Expect = 0.36
Identities = 20/51 (39%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = -1
Query: 482 PSGTIPAQHGLVDGSLSRPI-VISSPRGPNLTSSANAPPPPAHTSTPSVGR 531
PS TIP H L PI +S+PR P + + PPPPA + P + R
Sbjct: 543 PSATIPRNH------LRSPIPYLSTPRPPPPSPHFSPPPPPAPSPPPPLSR 409
>TC78364 similar to GP|9759266|dbj|BAB09587.1 diacylglycerol kinase-like
protein {Arabidopsis thaliana}, partial (76%)
Length = 1889
Score = 32.7 bits (73), Expect = 0.47
Identities = 32/83 (38%), Positives = 39/83 (46%), Gaps = 2/83 (2%)
Frame = -2
Query: 458 QASGKRPLGEEDPRPSPKRLNSS--EPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSA 515
QA G P P K L+ S PSGTIP G+ SRP SS R PN +
Sbjct: 805 QAKGNEP-------PQLKLLDRSFPVPSGTIPT-----GGTGSRPSWRSSVRHPN--TQP 668
Query: 516 NAPPPPAHTSTPSVGRISQLEKT 538
P PPA T T S R+S +++
Sbjct: 667 TVPSPPA-TITLSFSRVSLAQES 602
>BQ151301 similar to PIR|S34666|S346 glycine-rich protein - common tobacco,
partial (22%)
Length = 530
Score = 32.0 bits (71), Expect = 0.80
Identities = 21/67 (31%), Positives = 27/67 (39%), Gaps = 5/67 (7%)
Frame = +3
Query: 466 GEEDPRPSP-----KRLNSSEPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSANAPPP 520
G +DP P P K+ N + P P + G R + P P L + PPP
Sbjct: 60 GGKDPNPPPPPKNKKKKNHTTPPPPPPMRRG-------R*LCPPRPPPPPLPHGTHPPPP 218
Query: 521 PAHTSTP 527
P H TP
Sbjct: 219 PHHPPTP 239
>TC89118 weakly similar to PIR|T06098|T06098 hypothetical protein T5J17.90 -
Arabidopsis thaliana, partial (82%)
Length = 1208
Score = 31.6 bits (70), Expect = 1.0
Identities = 43/159 (27%), Positives = 69/159 (43%), Gaps = 10/159 (6%)
Frame = +1
Query: 451 KASLLKIQASGKRPLGEEDPRPSPKRLNSSEPSGTIPAQHGLVDGSLSRPIVISSPRGPN 510
KAS ++ R + +E + + N+ E A+ L LS +S R P
Sbjct: 25 KASCCDLKTPALRKMEDEMEKVTSSSSNNEEAE----AEAALQKKHLSMLDRLSK-RHPT 189
Query: 511 LTSSANAPPPPAHTSTPSVGRISQLEKTLAELRQEAQLESSVKA-------DQIASTEAD 563
+++ + PP TS+ + R SQL+ ++ E+Q SS D+I+ + +D
Sbjct: 190 KSTTTSTEPPTESTSS-FLSRFSQLKSSIESHLSESQFISSSDPSHLKSHFDKISQSISD 366
Query: 564 LENL---RSDLAKKDDLLSSAKTQAHLDAKALEEKLRSE 599
LE L S D+ SS KT + D K + L SE
Sbjct: 367 LEKLVAQSSYFLPSYDVRSSLKTVS--DFKRTLDNLSSE 477
>BQ751144 weakly similar to PIR|PQ0479|PQ04 pistil extensin-like protein
(clone pMG14) - common tobacco (fragment), partial (11%)
Length = 632
Score = 31.6 bits (70), Expect = 1.0
Identities = 21/61 (34%), Positives = 27/61 (43%), Gaps = 12/61 (19%)
Frame = +1
Query: 480 SEPSGTIPAQHGLVDGSLSRPIV-----ISSPRGPNLTSSANAPPPPAHT-------STP 527
S P T P+ H L+ + P SSP P SS +PPPP+ + STP
Sbjct: 190 SSPPSTSPSPHALLRSPTAPPAAPLSPPASSPSSPTRPSSLFSPPPPSPSRSRPPPPSTP 369
Query: 528 S 528
S
Sbjct: 370 S 372
>BQ141932 weakly similar to GP|6523547|emb hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (30%)
Length = 1338
Score = 31.6 bits (70), Expect = 1.0
Identities = 25/72 (34%), Positives = 31/72 (42%), Gaps = 2/72 (2%)
Frame = -2
Query: 470 PRPSPKRLNSSEPSGTIP--AQHGLVDGSLSRPIVISSPRGPNLTSSANAPPPPAHTSTP 527
PRP+P+ + PS TIP A + LSR SP P L + PP A P
Sbjct: 1073 PRPTPRATHPLSPSHTIPRLAPSPGLPAPLSRYYTSLSP--PPLHTHDTLTPPSAPAPLP 900
Query: 528 SVGRISQLEKTL 539
S+ L TL
Sbjct: 899 SISSRRLLRPTL 864
>TC87548 similar to PIR|T47553|T47553 peroxiredoxin-like protein -
Arabidopsis thaliana, partial (76%)
Length = 911
Score = 31.2 bits (69), Expect = 1.4
Identities = 20/58 (34%), Positives = 27/58 (46%)
Frame = +3
Query: 470 PRPSPKRLNSSEPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSANAPPPPAHTSTP 527
P+P P S S + + L D P+ SP P S+ N+P PP+ TSTP
Sbjct: 108 PKPKPSLPTSPSLSNSTASTQNLSDS----PLPPKSP--PPSPSATNSPNPPSPTSTP 263
>TC82997 homologue to GP|7293564|gb|AAF48937.1| CG7884 gene product
{Drosophila melanogaster}, partial (2%)
Length = 792
Score = 30.4 bits (67), Expect = 2.3
Identities = 19/60 (31%), Positives = 29/60 (47%)
Frame = +3
Query: 470 PRPSPKRLNSSEPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSANAPPPPAHTSTPSV 529
P+P P ++N+S S T + +L+ P S + P +SSA + P S PSV
Sbjct: 159 PQPPPPKMNTSPLSNTSQTSSAAIS-TLNEPSTNSESQSPPNSSSAFSALVPLPQSNPSV 335
>TC79709 similar to SP|Q39242|TRB2_ARATH Thioredoxin reductase 2 (EC
1.6.4.5) (NADPH-dependent thioredoxin reductase 2) (NTR
2)., partial (52%)
Length = 1121
Score = 30.4 bits (67), Expect = 2.3
Identities = 27/87 (31%), Positives = 37/87 (42%), Gaps = 8/87 (9%)
Frame = +1
Query: 504 SSPRGPNLTSSANAPPPPAHTSTPSVGRISQLEK-------TLAELRQEAQLESSVK-AD 555
S P P+ SSA APPP STP V +Q +L E Q S + A
Sbjct: 421 SPPLKPSSASSAAAPPPTPPLSTPPVPNSNQSSSKDGWPTTSLREANSPLQPTSRISPAS 600
Query: 556 QIASTEADLENLRSDLAKKDDLLSSAK 582
AS+ A+ N+ + + + SS K
Sbjct: 601 PTASSAANSWNVAVNSPRNSEPRSSPK 681
>TC91132 similar to GP|18087625|gb|AAL58942.1 AT4g22410/F7K2_7 {Arabidopsis
thaliana}, partial (12%)
Length = 684
Score = 30.4 bits (67), Expect = 2.3
Identities = 17/60 (28%), Positives = 26/60 (43%)
Frame = +2
Query: 435 FLARLAVSDCSRVIGDKASLLKIQASGKRPLGEEDPRPSPKRLNSSEPSGTIPAQHGLVD 494
FLA+ S R GD+ + KR +ED PSP +N + P + + +D
Sbjct: 149 FLAKNMASKRERATGDRVEEEGLDFESKRQRVDEDSSPSPPPVNIANPLSGLANNYADID 328
>BQ141543 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (15%)
Length = 1271
Score = 30.4 bits (67), Expect = 2.3
Identities = 19/60 (31%), Positives = 26/60 (42%)
Frame = -1
Query: 470 PRPSPKRLNSSEPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSANAPPPPAHTSTPSV 529
PRP + P G A+H + + P+ P P TS PPPP H ++ SV
Sbjct: 629 PRPPAAHVLGKNPGG---ARHMISPSRAAPPLFACPPSPPPPTS----PPPPPHPASASV 471
>BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, partial (2%)
Length = 1320
Score = 30.4 bits (67), Expect = 2.3
Identities = 17/48 (35%), Positives = 21/48 (43%)
Frame = -2
Query: 480 SEPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSANAPPPPAHTSTP 527
+ PS +PA G S S R P+ TS PPPP T+ P
Sbjct: 716 TNPSAFLPAARGSYSPSSPPTPPSFSSRAPSPTSPLPPPPPPLQTAGP 573
>BG587776 weakly similar to PIR|F86242|F86 unknown protein 98896-95855
[imported] - Arabidopsis thaliana, partial (23%)
Length = 608
Score = 30.0 bits (66), Expect = 3.0
Identities = 23/102 (22%), Positives = 49/102 (47%), Gaps = 2/102 (1%)
Frame = +2
Query: 521 PAH-TSTPSVGRISQLEKTLAELRQEAQLESSVKADQIASTEADLENLRSDLAKK-DDLL 578
PAH +S+ S+G + Q + ++E + +A+ E + + + K+ D+ L
Sbjct: 182 PAHKSSSSSLGSVEQKTAIEKQRKEEEKKRRQQEAELKLIAEETAKRVEEAIRKRVDESL 361
Query: 579 SSAKTQAHLDAKALEEKLRSEDAVAAVSERGRGFFLAKAQVK 620
SS + + + + E + + D VAA ++ + L +A+ K
Sbjct: 362 SSEEVRVEIQRRLEEGRKKLNDEVAAQLQKEKEAALIEAKQK 487
>BF638182 weakly similar to PIR|T45594|T45 glucosidase-like protein -
Arabidopsis thaliana, partial (46%)
Length = 674
Score = 29.6 bits (65), Expect = 4.0
Identities = 25/91 (27%), Positives = 36/91 (39%), Gaps = 18/91 (19%)
Frame = +3
Query: 464 PLGEEDPRPSPKRLNSSEPSGTIPAQHGLVDGSLSRPIVIS-----SPRGPNLTSSANAP 518
P P+PSP S T+ + + S+ PI S SP +L S + P
Sbjct: 99 PTTSHHPQPSPLSSKPKPQSPTLKSSTQTLPSSVPSPIQTSPSP*LSPTPTSLPSQNSPP 278
Query: 519 -------------PPPAHTSTPSVGRISQLE 536
P P+ T +PSV + SQL+
Sbjct: 279 LKNGSPPTSSLSTPKPSSTVSPSVTKSSQLQ 371
>TC79953 similar to GP|20279471|gb|AAM18751.1 unknown protein {Oryza sativa
(japonica cultivar-group)}, partial (25%)
Length = 899
Score = 29.6 bits (65), Expect = 4.0
Identities = 24/83 (28%), Positives = 36/83 (42%), Gaps = 11/83 (13%)
Frame = +1
Query: 16 AATPLRCVPPVRPPPPGGVPL-----------EEKDVIAFRTRTWKSLSAPAARDLPAGT 64
AA P P PPPPG VP+ + D I++R + AP A T
Sbjct: 427 AAPPYHNYPNYYPPPPGTVPMPLPAPYDHHHRQAMDPISWRYQQQVPAPAPYVEHQKAVT 606
Query: 65 MPNQRSSLEKESSIWEISTESGG 87
+ N +++KE+ + E E+ G
Sbjct: 607 IKND-VNIKKETLMIEPDEENPG 672
>TC91842 similar to GP|21427461|gb|AAM53243.1 actin-related protein 3
{Arabidopsis thaliana}, partial (78%)
Length = 1021
Score = 29.6 bits (65), Expect = 4.0
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = -3
Query: 509 PNLTSSANAPPPPAHTSTPSVGRISQLEKTLAELRQEAQLESSVKADQIAS 559
P+ T + P P T+TP IS L+ ++A E ++ S+VK+ I S
Sbjct: 557 PSATGTTCVAPSPISTTTPV---ISHLDXSVASSESEHRIHSNVKSRHIKS 414
>BI272602 GP|11602812|db avenaIII {Gallus gallus}, partial (3%)
Length = 488
Score = 29.6 bits (65), Expect = 4.0
Identities = 28/104 (26%), Positives = 46/104 (43%), Gaps = 17/104 (16%)
Frame = +1
Query: 497 LSRPIVISSPRGP------NLTSSANAPPPPAHTSTP------SVGRI-----SQLEKTL 539
+++ I ISSP P N+T+S PPPP T+ P +VG I + + KT+
Sbjct: 49 VAQNIQISSPASPTSNELANITTSPTPPPPPPPTARPHNNHTTNVGGIITRSQNNIVKTI 228
Query: 540 AELRQEAQLESSVKADQIASTEADLENLRSDLAKKDDLLSSAKT 583
+L + ++ I D + RS + + D L + T
Sbjct: 229 KKLNLHVRPLCPIEPSNITQALRD-HDWRSIMQDEFDALQNNNT 357
>BE998536 similar to GP|2598593|emb| MtN12 {Medicago truncatula}, partial
(64%)
Length = 448
Score = 29.3 bits (64), Expect = 5.2
Identities = 19/54 (35%), Positives = 23/54 (42%)
Frame = +3
Query: 470 PRPSPKRLNSSEPSGTIPAQHGLVDGSLSRPIVISSPRGPNLTSSANAPPPPAH 523
P P P + P T P HG G S P + SP P+ +PPPP H
Sbjct: 153 PHPKPGYHSPPPPGHTYPP-HGPHPGYHSPPAPVDSPPTPDY--YYKSPPPPYH 305
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,986,890
Number of Sequences: 36976
Number of extensions: 324852
Number of successful extensions: 2517
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 2225
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2485
length of query: 681
length of database: 9,014,727
effective HSP length: 103
effective length of query: 578
effective length of database: 5,206,199
effective search space: 3009183022
effective search space used: 3009183022
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0103.2