BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.10
(418 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF644046 56 2e-08
TC88550 similar to GP|17529098|gb|AAL38759.1 unknown protein {Ar... 36 0.024
BQ135365 weakly similar to GP|17834086|gb| haymaker protein {Mus... 32 0.46
TC79289 weakly similar to GP|7298980|gb|AAF54183.1| CG10903 gene... 32 0.59
TC87973 weakly similar to PIR|D86282|D86282 protein F10B6.26 [im... 31 1.0
TC76863 homologue to GP|4165323|dbj|BAA37150.1 p-type H+-ATPase ... 31 1.0
CA920913 similar to GP|12322736|gb putative protein kinase; 7090... 30 1.7
BG450291 similar to GP|14335084|gb| At1g11480/T23J18_15 {Arabido... 30 1.7
BE203527 homologue to GP|7705248|gb| plasma membrane H(+)-ATPase... 30 2.3
BQ751144 weakly similar to PIR|PQ0479|PQ04 pistil extensin-like ... 29 2.9
BF640720 homologue to PIR|S52728|S52 H+-transporting ATPase (EC ... 29 2.9
BE998536 similar to GP|2598593|emb| MtN12 {Medicago truncatula},... 29 2.9
TC92150 weakly similar to PIR|T47938|T47938 RING finger protein ... 29 2.9
CB894422 29 2.9
TC81549 similar to GP|6630553|gb|AAF19572.1| unknown protein {Ar... 29 3.9
TC81307 weakly similar to GP|4335772|gb|AAD17449.1| unknown prot... 29 3.9
TC77408 similar to GP|12006855|gb|AAG44951.1 POZ/BTB containing-... 29 3.9
TC78364 similar to GP|9759266|dbj|BAB09587.1 diacylglycerol kina... 29 3.9
BF004047 weakly similar to GP|15027957|gb| putative arginine/ser... 29 3.9
BQ141932 weakly similar to GP|6523547|emb hydroxyproline-rich gl... 29 3.9
>BF644046
Length = 597
Score = 56.2 bits (134), Expect = 2e-08
Identities = 24/52 (46%), Positives = 36/52 (69%)
Frame = +3
Query: 45 YVYGYMFPNLKIKFPFSPFICSVLHNINVAPSQLHPNSWAFLRCFEILCDAI 96
++Y ++F ++ KFPF+ F C L +NVA SQLHPN AF+ FEI C+++
Sbjct: 9 HMYSFVFEDIGFKFPFTNFECDFLKALNVASSQLHPNCCAFMCGFEISCESL 164
>TC88550 similar to GP|17529098|gb|AAL38759.1 unknown protein {Arabidopsis
thaliana}, partial (29%)
Length = 888
Score = 36.2 bits (82), Expect = 0.024
Identities = 42/157 (26%), Positives = 63/157 (39%), Gaps = 5/157 (3%)
Frame = -2
Query: 239 NLKGDKASLLRIQAGRKRPLGEEDPRPSPKRLNSGEPSGTIPAQHGLVDGSLSRPTVISS 298
N++GD A + ++ PRP PKRL S + SL P +++
Sbjct: 497 NIEGDFAITSKRPFFISSAASKKRPRP-PKRLRS------------MTSISLRSPGKLNT 357
Query: 299 PLGPNLTSSANAPPPPVHTSTLSSGSVSRGFSCSL-TLTSAQMARMDEVVKQRGLDNISE 357
P L N PP+ TS + S SV F CS T AQ+ R V
Sbjct: 356 PWSDELFDEPNVVDPPLSTSLVISSSVYSLFDCSTSTGFPAQVLRSSSVFSH--FRTNFP 183
Query: 358 GAVARVLH----AFHCYRRSAQDADKLRSEVVRLRAL 390
G+ + VLH F RS++ + L S ++ + +
Sbjct: 182 GSTSSVLH*DFTTFLVEHRSSEPEELLLSSSLQTQPI 72
>BQ135365 weakly similar to GP|17834086|gb| haymaker protein {Mus musculus},
partial (7%)
Length = 1147
Score = 32.0 bits (71), Expect = 0.46
Identities = 23/72 (31%), Positives = 34/72 (46%), Gaps = 5/72 (6%)
Frame = +3
Query: 97 HQEPEPSTFFYFYRVSVQPSPQRSWVSLEARPGRQRFNPIWPSITIALAHKFF-----RV 151
H + P F FY++ ++P +VS A PGR R PI S ++L FF R
Sbjct: 330 HPKSPPLFFLAFYQLCIEPRWSLHYVSFAAAPGRCRPIPIVRS-CVSLFFMFFWPLPLRP 506
Query: 152 VVSHVYPEVFTH 163
++Y + TH
Sbjct: 507 FSLYLYTFLMTH 542
>TC79289 weakly similar to GP|7298980|gb|AAF54183.1| CG10903 gene product
{Drosophila melanogaster}, partial (83%)
Length = 1098
Score = 31.6 bits (70), Expect = 0.59
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 6/74 (8%)
Frame = -1
Query: 260 EEDPRPSPKRLNSGEPSGTIPAQHGLVDGSLSRPT------VISSPLGPNLTSSANAPPP 313
+ P+P P + PS + ++G++SRPT VI+SPL P+ P
Sbjct: 348 KRSPKP*PMSPSKRSPSTSRSNAISSIEGAMSRPTQWCPLSVITSPLSPD--------PQ 193
Query: 314 PVHTSTLSSGSVSR 327
P+ S+L + S+ +
Sbjct: 192 PMSRSSLGTPSLGK 151
>TC87973 weakly similar to PIR|D86282|D86282 protein F10B6.26 [imported] -
Arabidopsis thaliana, partial (82%)
Length = 1073
Score = 30.8 bits (68), Expect = 1.0
Identities = 16/49 (32%), Positives = 27/49 (54%), Gaps = 1/49 (2%)
Frame = -3
Query: 76 SQLHPNSWAFLRCFEILCDAIHQEPEPSTFFYFYR-VSVQPSPQRSWVS 123
S LHP+ WA+ CF IH +P S++ +F+ + + + QR +S
Sbjct: 669 SFLHPSIWAYFFCF------IHSQPFSSSYVHFWPIIQLLANKQREHIS 541
>TC76863 homologue to GP|4165323|dbj|BAA37150.1 p-type H+-ATPase {Vicia
faba}, partial (33%)
Length = 1376
Score = 30.8 bits (68), Expect = 1.0
Identities = 20/55 (36%), Positives = 27/55 (48%), Gaps = 11/55 (20%)
Frame = +1
Query: 46 VYGYMFPNLKIKFPFSPFICSVLHNIN-----------VAPSQLHPNSWAFLRCF 89
V+G+MF L KF FSPF+ ++ +N V PS L P+SW F
Sbjct: 73 VFGFMFIALIWKFDFSPFMVLIIAILNDGTIMTISKDRVVPSPL-PDSWKLKEIF 234
>CA920913 similar to GP|12322736|gb putative protein kinase; 70907-69052
{Arabidopsis thaliana}, partial (23%)
Length = 713
Score = 30.0 bits (66), Expect = 1.7
Identities = 27/89 (30%), Positives = 41/89 (45%), Gaps = 1/89 (1%)
Frame = -2
Query: 257 PLGEEDPRPSPKRLNSGEPSGTI-PAQHGLVDGSLSRPTVISSPLGPNLTSSANAPPPPV 315
PL E P + L S +G I PA + S SRP+V P+ P S PP
Sbjct: 352 PLQEPAPLSGKEMLRSPSLNGIICPAPR--LSYSPSRPSVALIPISPPRWSGVPVLPPRA 179
Query: 316 HTSTLSSGSVSRGFSCSLTLTSAQMARMD 344
+STLS + R S + ++++ A ++
Sbjct: 178 CSSTLSLVELDRQESRKSSSSASRRASVE 92
>BG450291 similar to GP|14335084|gb| At1g11480/T23J18_15 {Arabidopsis
thaliana}, partial (12%)
Length = 680
Score = 30.0 bits (66), Expect = 1.7
Identities = 25/98 (25%), Positives = 40/98 (40%), Gaps = 4/98 (4%)
Frame = +2
Query: 262 DPRPSPKRLNSGEPSGTIPAQHGLVDGSLSRPTVISSPLGPNLTSSANAPP----PPVHT 317
+P +++N P T+P +HG + S SP P N P P VH
Sbjct: 239 EPTQGYRQVNDSVPVETVPQRHGHANFVKSVSVGTESPKDPGQRPKLNLKPLKPRPEVHE 418
Query: 318 STLSSGSVSRGFSCSLTLTSAQMARMDEVVKQRGLDNI 355
+ +S R L R + V+K+RG+D++
Sbjct: 419 QSEASKERDRN-----ALFGGARPR-ELVLKERGVDDV 514
>BE203527 homologue to GP|7705248|gb| plasma membrane H(+)-ATPase precursor
{Vicia faba}, partial (14%)
Length = 417
Score = 29.6 bits (65), Expect = 2.3
Identities = 19/55 (34%), Positives = 27/55 (48%), Gaps = 11/55 (20%)
Frame = +1
Query: 46 VYGYMFPNLKIKFPFSPFICSVLHNIN-----------VAPSQLHPNSWAFLRCF 89
V+G+MF L KF FSPF+ ++ +N V PS + P+SW F
Sbjct: 91 VFGFMFIALIWKFDFSPFMVLIIAILNDGTIMTISKDRVKPSPM-PDSWKLREIF 252
>BQ751144 weakly similar to PIR|PQ0479|PQ04 pistil extensin-like protein
(clone pMG14) - common tobacco (fragment), partial (11%)
Length = 632
Score = 29.3 bits (64), Expect = 2.9
Identities = 16/45 (35%), Positives = 20/45 (43%), Gaps = 5/45 (11%)
Frame = +1
Query: 275 PSGTIPAQHGLVDGSLSRPTV-----ISSPLGPNLTSSANAPPPP 314
P T P+ H L+ + P SSP P SS +PPPP
Sbjct: 196 PPSTSPSPHALLRSPTAPPAAPLSPPASSPSSPTRPSSLFSPPPP 330
>BF640720 homologue to PIR|S52728|S52 H+-transporting ATPase (EC 3.6.1.35) -
kidney bean, partial (24%)
Length = 689
Score = 29.3 bits (64), Expect = 2.9
Identities = 19/55 (34%), Positives = 27/55 (48%), Gaps = 11/55 (20%)
Frame = +2
Query: 46 VYGYMFPNLKIKFPFSPFICSVLHNIN-----------VAPSQLHPNSWAFLRCF 89
V+G+MF L KF F+PF+ ++ +N V PS L P+SW F
Sbjct: 293 VFGFMFIALIWKFDFAPFMVLIIAILNDGTIMTISKDRVKPSPL-PDSWKLREIF 454
>BE998536 similar to GP|2598593|emb| MtN12 {Medicago truncatula}, partial
(64%)
Length = 448
Score = 29.3 bits (64), Expect = 2.9
Identities = 19/54 (35%), Positives = 23/54 (42%)
Frame = +3
Query: 263 PRPSPKRLNSGEPSGTIPAQHGLVDGSLSRPTVISSPLGPNLTSSANAPPPPVH 316
P P P + P T P HG G S P + SP P+ +PPPP H
Sbjct: 153 PHPKPGYHSPPPPGHTYPP-HGPHPGYHSPPAPVDSPPTPDY--YYKSPPPPYH 305
>TC92150 weakly similar to PIR|T47938|T47938 RING finger protein -
Arabidopsis thaliana, partial (51%)
Length = 595
Score = 29.3 bits (64), Expect = 2.9
Identities = 20/68 (29%), Positives = 33/68 (48%), Gaps = 6/68 (8%)
Frame = +1
Query: 289 SLSRPTVISSPLGPNLTS------SANAPPPPVHTSTLSSGSVSRGFSCSLTLTSAQMAR 342
SLS P+ + SP +L+S + +PPPP T T + + S S + +S M
Sbjct: 58 SLSSPSSVLSPSPSSLSSISPTSSTPTSPPPPYLTPTSTVPLSLQSSSVSFSQSSLSMTS 237
Query: 343 MDEVVKQR 350
E++ +R
Sbjct: 238 PKEILHRR 261
>CB894422
Length = 812
Score = 29.3 bits (64), Expect = 2.9
Identities = 25/70 (35%), Positives = 36/70 (50%), Gaps = 3/70 (4%)
Frame = -2
Query: 278 TIPAQHGLVDGSLSRPTVISSPLGPNLTSSANAPPPPVHTSTLSSGSV---SRGFSCSLT 334
TI + +D L+ P ++S P GP L S P PVH +LSS + S S S++
Sbjct: 211 TIESSSSKLDWLLT-PAMLSRP-GPGLLCSKYPHPLPVHLISLSSQATKNFSFHVSVSVS 38
Query: 335 LTSAQMARMD 344
L+S M + D
Sbjct: 37 LSSLHMMQGD 8
>TC81549 similar to GP|6630553|gb|AAF19572.1| unknown protein {Arabidopsis
thaliana}, partial (7%)
Length = 1309
Score = 28.9 bits (63), Expect = 3.9
Identities = 16/59 (27%), Positives = 24/59 (40%)
Frame = +2
Query: 260 EEDPRPSPKRLNSGEPSGTIPAQHGLVDGSLSRPTVISSPLGPNLTSSANAPPPPVHTS 318
E PRP P+ ++ SGT Q P + SP PN ++ +H+S
Sbjct: 416 EHQPRPLPQHISGQHISGTWVPQSTPAGIGYGHPETMMSPFNPNQVGIHSSSTMYLHSS 592
>TC81307 weakly similar to GP|4335772|gb|AAD17449.1| unknown protein
{Arabidopsis thaliana}, partial (31%)
Length = 1171
Score = 28.9 bits (63), Expect = 3.9
Identities = 22/53 (41%), Positives = 25/53 (46%), Gaps = 1/53 (1%)
Frame = +2
Query: 267 PKRLNSGEPSGTIPAQHGLVDGSLSRPTVI-SSPLGPNLTSSANAPPPPVHTS 318
P LN E S P L S S ++ SS L P+ TSSA P PP TS
Sbjct: 101 PSSLNPNESSTLTPVTSLLSPSSSSFLSLSPSSSLPPSSTSSAPHPTPPPSTS 259
>TC77408 similar to GP|12006855|gb|AAG44951.1 POZ/BTB containing-protein
AtPOB1 {Arabidopsis thaliana}, partial (85%)
Length = 2287
Score = 28.9 bits (63), Expect = 3.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2
Query: 60 FSPFICSVLHNINVAPSQLHPNSWA 84
F P +C ++H I++ PS N WA
Sbjct: 2199 FCPILCHIMHMIDIKPSLQKNNFWA 2125
>TC78364 similar to GP|9759266|dbj|BAB09587.1 diacylglycerol kinase-like
protein {Arabidopsis thaliana}, partial (76%)
Length = 1889
Score = 28.9 bits (63), Expect = 3.9
Identities = 25/68 (36%), Positives = 31/68 (44%)
Frame = -2
Query: 259 GEEDPRPSPKRLNSGEPSGTIPAQHGLVDGSLSRPTVISSPLGPNLTSSANAPPPPVHTS 318
G E P+ + PSGTIP G+ SRP+ SS PN + +PP T
Sbjct: 796 GNEPPQLKLLDRSFPVPSGTIPT-----GGTGSRPSWRSSVRHPNTQPTVPSPPA---TI 641
Query: 319 TLSSGSVS 326
TLS VS
Sbjct: 640 TLSFSRVS 617
>BF004047 weakly similar to GP|15027957|gb| putative arginine/serine-rich
protein {Arabidopsis thaliana}, partial (11%)
Length = 835
Score = 28.9 bits (63), Expect = 3.9
Identities = 20/62 (32%), Positives = 31/62 (49%), Gaps = 5/62 (8%)
Frame = -3
Query: 276 SGTIPAQHGLVDGSLSRPTVISSPLG-----PNLTSSANAPPPPVHTSTLSSGSVSRGFS 330
S ++ + S S P +I P G P+ +SS+ +PPPP TS ++G+ R S
Sbjct: 245 SSSLSSSPSTSPSSRSPPPLIFPPSGSPPCPPSTSSSSRSPPPPTSTSP-TTGTPPRSTS 69
Query: 331 CS 332
S
Sbjct: 68 PS 63
>BQ141932 weakly similar to GP|6523547|emb hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (30%)
Length = 1338
Score = 28.9 bits (63), Expect = 3.9
Identities = 19/55 (34%), Positives = 27/55 (48%)
Frame = -2
Query: 263 PRPSPKRLNSGEPSGTIPAQHGLVDGSLSRPTVISSPLGPNLTSSANAPPPPVHT 317
PRP+P+ + PS TIP L+ + +PL TS + PPP+HT
Sbjct: 1073 PRPTPRATHPLSPSHTIP--------RLAPSPGLPAPLSRYYTSLS---PPPLHT 942
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.134 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,355,773
Number of Sequences: 36976
Number of extensions: 251165
Number of successful extensions: 1653
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 1594
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1645
length of query: 418
length of database: 9,014,727
effective HSP length: 99
effective length of query: 319
effective length of database: 5,354,103
effective search space: 1707958857
effective search space used: 1707958857
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0103.10