BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0101.12
(310 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB892913 similar to GP|1769897|emb lectin receptor kinase {Arabi... 56 1e-18
TC93065 61 5e-10
BI262818 weakly similar to GP|6642775|gb| gag-pol polyprotein {V... 55 3e-08
BQ122291 42 4e-04
BF648378 similar to GP|9294677|dbj| gene_id:F14O13.28~pir||T2263... 31 0.69
TC80784 similar to PIR|T50352|T50352 ornithine--oxo-acid transam... 30 1.5
TC88164 GP|3763933|gb|AAC64313.1| unknown protein {Arabidopsis t... 30 1.5
TC81207 similar to GP|21322752|dbj|BAB78536. cold shock protein-... 30 1.5
TC87383 similar to GP|19168656|emb|CAD26175. DNA-DIRECTED RNA PO... 29 2.6
TC85208 homologue to PIR|F86214|F86214 protein T6D22.2 [imported... 29 2.6
TC85137 GP|3869088|dbj|BAA34348.1 elongation factor-1 alpha {Nic... 29 2.6
TC77240 homologue to GP|1370196|emb|CAA98175.1 RAB8D {Lotus japo... 28 3.4
TC85141 homologue to PIR|F86214|F86214 protein T6D22.2 [imported... 28 3.4
TC87100 homologue to GP|9293891|dbj|BAB01794.1 contains similari... 28 3.4
TC91139 similar to GP|15292825|gb|AAK92781.1 unknown protein {Ar... 28 4.5
TC88387 similar to GP|13357253|gb|AAK20050.1 putative zinc finge... 28 5.9
TC82467 similar to GP|9758269|dbj|BAB08768.1 emb|CAA18192.1~gene... 28 5.9
TC78556 similar to GP|9759255|dbj|BAB09690.1 contains similarity... 28 5.9
BF641303 similar to GP|9758269|dbj emb|CAA18192.1~gene_id:MBG8.1... 28 5.9
>CB892913 similar to GP|1769897|emb lectin receptor kinase {Arabidopsis
thaliana}, partial (35%)
Length = 837
Score = 56.2 bits (134), Expect(2) = 1e-18
Identities = 36/107 (33%), Positives = 59/107 (54%), Gaps = 4/107 (3%)
Frame = +3
Query: 1 MIAFLKSIDIKTWKAVVKGWKHPVKAQAEGSTSTPEEKPEDELTSAE----EEASLENSK 56
M+A L + D+ W+ V KG+K E + ED LT A+ +++ + K
Sbjct: 171 MMALLGAHDV--WEVVEKGYK--------------ESQDEDSLTKAQRDTLKDSRKRDKK 302
Query: 57 AMNAIFNAVDKNMFRLINTCTVAKDAWEILKTTHEGTTRVRMSRLQM 103
A+ I+ A+D++ F I+ T AK+AWE LKT+ +G +V+ RLQ+
Sbjct: 303 ALFLIYQALDEDEFEKISNATSAKEAWEKLKTSCQGEDKVKKVRLQI 443
Score = 54.3 bits (129), Expect(2) = 1e-18
Identities = 32/84 (38%), Positives = 47/84 (55%)
Frame = +1
Query: 117 ERISEYHMRVRDLSNASFALGEPMSDEKLVRKILRSVTSKFAMKVIAIEEAQDISSLKVD 176
ER+ Y RV +SN E + D +++ KILRS+ KF V IEE +D+ ++ ++
Sbjct: 472 ERVRVYFSRVISISNQLKRNSEKLEDVRIMEKILRSLDPKFEHIVEKIEETKDLETMTIE 651
Query: 177 ELIGSLQTYKLKLGEKPEKKTKSI 200
+L GSLQ Y E+ KK K I
Sbjct: 652 KLQGSLQAY-----EEKHKKKKDI 708
>TC93065
Length = 783
Score = 61.2 bits (147), Expect = 5e-10
Identities = 49/208 (23%), Positives = 85/208 (40%), Gaps = 1/208 (0%)
Frame = +2
Query: 104 LTTRFENLTMTEDERISEYHMRVRDLSNASFALGEPMSDEKLVRKILRSVTSKFAMKVIA 163
L FE L M E E + E+ R+ + LGE +SD+++V KIL + F K+ +
Sbjct: 215 LRREFEALKMKETETVREFSDRISKVVTQIRLLGEDLSDQRVVEKILVCLPEMFEAKISS 394
Query: 164 IEEAQDISSLKVDELIGSLQTYKLKLGEKPEKKTKSIAFVSNTTEGDDADSESENLSEAL 223
+EE ++ S + V EL+ +LQ + + + E+ + AF++N
Sbjct: 395 LEENKNFSEITVAELVNALQASEQRRSLRMEENVEG-AFLANN----------------- 520
Query: 224 ALLARKFNRALRKIDRRTRPNVQDIKSDNSKSYNSQRKVKEEDKSGQNKGVQCHECEGYG 283
+ N++ + ++ P K D + GV+C C G
Sbjct: 521 ----KGKNQSFKSFGKKKFPPCPHCKKDTHL----------DKFCWYRPGVKCRACNQLG 658
Query: 284 HIRSECATYLKKQ-KKGMVVTWSDEDSE 310
H+ C +Q ++ VV ED E
Sbjct: 659 HVEKVCKNKTNQQEQEARVVEHHQEDEE 742
>BI262818 weakly similar to GP|6642775|gb| gag-pol polyprotein {Vitis
vinifera}, partial (24%)
Length = 615
Score = 55.5 bits (132), Expect = 3e-08
Identities = 32/97 (32%), Positives = 46/97 (46%)
Frame = +1
Query: 48 EEASLENSKAMNAIFNAVDKNMFRLINTCTVAKDAWEILKTTHEGTTRVRMSRLQMLTTR 107
+E SKA +F AV + +F I T A + W LKT +EG R+R + L
Sbjct: 325 KERKTRKSKARATLFAAVSEEIFTRIMTIKSAFEIWNFLKTEYEGDERIRGMQALNLIRE 504
Query: 108 FENLTMTEDERISEYHMRVRDLSNASFALGEPMSDEK 144
FE M E E I EY ++ ++N LG + D +
Sbjct: 505 FEMQKMKESETIKEYANKLISIANKVRLLGSELXDSR 615
>BQ122291
Length = 487
Score = 41.6 bits (96), Expect = 4e-04
Identities = 25/113 (22%), Positives = 55/113 (48%)
Frame = -2
Query: 72 LINTCTVAKDAWEILKTTHEGTTRVRMSRLQMLTTRFENLTMTEDERISEYHMRVRDLSN 131
L++ + + +W++ H +R + L + ++T +SE+ R+R +S
Sbjct: 384 LLSRFVLLRHSWQVWDEIHSYCFTQMKTRSRQLRSELRSITKGS-RTVSEFIARIRAISE 208
Query: 132 ASFALGEPMSDEKLVRKILRSVTSKFAMKVIAIEEAQDISSLKVDELIGSLQT 184
+ ++G+P+S L+ +L ++ +F V ++ ++ SL DEL L T
Sbjct: 207 SLASIGDPVSHRDLIEVVLEALPEEFDPIVASVNAKSEVVSL--DELESQLLT 55
>BF648378 similar to GP|9294677|dbj| gene_id:F14O13.28~pir||T22638~similar to
unknown protein {Arabidopsis thaliana}, partial (8%)
Length = 651
Score = 30.8 bits (68), Expect = 0.69
Identities = 29/113 (25%), Positives = 57/113 (49%), Gaps = 11/113 (9%)
Frame = +2
Query: 166 EAQDISSLKVDELIGSLQTYKLK---LGEKPEKKTKSIAFVSNTTEGDDADSES---ENL 219
E +DIS++K+DE + + L E K K + + TE D+++SES +++
Sbjct: 176 ETEDISNIKIDEEYARRFEHNKRREDLQRYEELKKKGVIGSPSHTEDDESESESSSDDDV 355
Query: 220 SEALALLARKFNRALRKIDRR----TRPNVQDIKSDNSKSYNS-QRKVKEEDK 267
+ ++F AL K+ ++ + +V+ +SD+S S K K+++K
Sbjct: 356 NNFNTKSDKEFFDALIKVKKKDPVLKQKDVKLFESDHSSEDESDDEKSKDKEK 514
>TC80784 similar to PIR|T50352|T50352 ornithine--oxo-acid transaminase (EC
2.6.1.13) [imported] - fission yeast, partial (36%)
Length = 1206
Score = 29.6 bits (65), Expect = 1.5
Identities = 16/35 (45%), Positives = 21/35 (59%)
Frame = +3
Query: 215 ESENLSEALALLARKFNRALRKIDRRTRPNVQDIK 249
+ ENL E A+L KF ALRKID P +Q ++
Sbjct: 291 KEENLVEKAAVLGEKFRSALRKID---SPLIQTVR 386
>TC88164 GP|3763933|gb|AAC64313.1| unknown protein {Arabidopsis thaliana},
partial (1%)
Length = 879
Score = 29.6 bits (65), Expect = 1.5
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = -3
Query: 220 SEALALLARKFNRALRKIDRRTRPNVQDIKSDNSKSYNSQRKVKEEDKSGQNKGVQCHEC 279
SE+L+LL R R LR RRT+ + + + N + K +EE+ S V C C
Sbjct: 184 SESLSLLLRLLRRLLRL--RRTKEEAKTTRDKHGCGGNGRVKEEEENISDIKSHVSCTFC 11
>TC81207 similar to GP|21322752|dbj|BAB78536. cold shock protein-1 {Triticum
aestivum}, partial (39%)
Length = 630
Score = 29.6 bits (65), Expect = 1.5
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = +3
Query: 273 GVQCHECEGYGHIRSECAT 291
G C+ C G GHI +CAT
Sbjct: 555 GTSCYRCGGVGHIARDCAT 611
>TC87383 similar to GP|19168656|emb|CAD26175. DNA-DIRECTED RNA POLYMERASE II
{Encephalitozoon cuniculi}, partial (0%)
Length = 1247
Score = 28.9 bits (63), Expect = 2.6
Identities = 52/231 (22%), Positives = 88/231 (37%), Gaps = 39/231 (16%)
Frame = -2
Query: 101 LQMLTTRFENLTMTEDERISEYHMRVRDLSNASFALGEPM---------SDEKLVRKILR 151
+QM R ++ E E++ +++++ NA AL E S + L + R
Sbjct: 949 VQMPPRRQNERSLQEMEKMRR*IQQLQEIVNAQQALLEAQQKRFKDHVSSSDSLSSRSSR 770
Query: 152 SVTSKFAMKVIAIEEAQDISSLKVDELIGSLQT------YKLKLGEKPEK----KTKSIA 201
S +F M I + +L+ D+L+ LQ YK L E+ K K K +A
Sbjct: 769 SQRREFQMNDIK*DIPDFEGNLQPDDLLDWLQIMERLFKYKEVLEEQKVKIVAAKLKKLA 590
Query: 202 FV----SNTTEGDDADSESENLSEALALLARKFNRALRKIDRRTRPNVQDIKS------- 250
+ + S+ + + L RK+ D T+P + S
Sbjct: 589 SIWWENVKRRRKREGKSKIKTWEKMRQKLTRKYLPPHYYQDNYTQPQLSKKSSYRHFSPT 410
Query: 251 ----DNSKSYNSQR----KVKEEDKSGQNKGV-QCHECEGYGHIRSECATY 292
D+ K Q K + + + N + +C C+GYGHI +C Y
Sbjct: 409 KNQIDSHKPLIHQPIFTFKPRHKKITEINTNIPKCVMCQGYGHIAIDCVNY 257
>TC85208 homologue to PIR|F86214|F86214 protein T6D22.2 [imported] -
Arabidopsis thaliana, partial (46%)
Length = 2287
Score = 28.9 bits (63), Expect = 2.6
Identities = 24/83 (28%), Positives = 37/83 (43%), Gaps = 5/83 (6%)
Frame = +2
Query: 171 SSLKVDELIGSLQTYKLKLGEKPEKKTKSIAFVS-NTTEGDDADSESENL----SEALAL 225
S + DE++ + +Y K+G P+K I FV + EGD+ S NL L
Sbjct: 959 SKARYDEIVKEVSSYLKKVGYNPDK----IPFVPISGFEGDNMIERSTNLDWYKGPTLLE 1126
Query: 226 LARKFNRALRKIDRRTRPNVQDI 248
+ N R D+ R +QD+
Sbjct: 1127 ALDQINEPKRPTDKPLRLPLQDV 1195
>TC85137 GP|3869088|dbj|BAA34348.1 elongation factor-1 alpha {Nicotiana
paniculata}, partial (56%)
Length = 1023
Score = 28.9 bits (63), Expect = 2.6
Identities = 24/83 (28%), Positives = 37/83 (43%), Gaps = 5/83 (6%)
Frame = +1
Query: 171 SSLKVDELIGSLQTYKLKLGEKPEKKTKSIAFVS-NTTEGDDADSESENL----SEALAL 225
S + DE++ + +Y K+G P+K I FV + EGD+ S NL L
Sbjct: 724 SKARYDEIVKEVSSYLKKVGYNPDK----IPFVPISGFEGDNMIERSTNLDWYKGPTLLE 891
Query: 226 LARKFNRALRKIDRRTRPNVQDI 248
+ N R D+ R +QD+
Sbjct: 892 ALDQINEPKRPTDKPLRLPLQDV 960
>TC77240 homologue to GP|1370196|emb|CAA98175.1 RAB8D {Lotus japonicus},
complete
Length = 1148
Score = 28.5 bits (62), Expect = 3.4
Identities = 20/78 (25%), Positives = 28/78 (35%)
Frame = +1
Query: 218 NLSEALALLARKFNRALRKIDRRTRPNVQDIKSDNSKSYNSQRKVKEEDKSGQNKGVQCH 277
N+ E +AR + L D + P I D++ +G + Q
Sbjct: 715 NVEEVFFSIARDIKQRLADTDNKAEPTTIKINQDSA--------------TGSGQAAQKS 852
Query: 278 ECEGYGHIRSECATYLKK 295
C G GH AT LKK
Sbjct: 853 ACCG*GHFYKLVATLLKK 906
>TC85141 homologue to PIR|F86214|F86214 protein T6D22.2 [imported] -
Arabidopsis thaliana, partial (47%)
Length = 1881
Score = 28.5 bits (62), Expect = 3.4
Identities = 24/83 (28%), Positives = 37/83 (43%), Gaps = 5/83 (6%)
Frame = +3
Query: 171 SSLKVDELIGSLQTYKLKLGEKPEKKTKSIAFVS-NTTEGDDADSESENL----SEALAL 225
S + DE++ + +Y K+G P+K I FV + EGD+ S NL L
Sbjct: 564 SKSRYDEIVKEVSSYMKKVGYNPDK----IPFVPISGFEGDNMIERSTNLDWYKGPTLLE 731
Query: 226 LARKFNRALRKIDRRTRPNVQDI 248
+ N R D+ R +QD+
Sbjct: 732 ALDQINEPKRPSDKPLRLPLQDV 800
>TC87100 homologue to GP|9293891|dbj|BAB01794.1 contains similarity to
uridylate kinase~gene_id:MVE11.4 {Arabidopsis thaliana},
partial (58%)
Length = 891
Score = 28.5 bits (62), Expect = 3.4
Identities = 20/73 (27%), Positives = 38/73 (51%), Gaps = 7/73 (9%)
Frame = -1
Query: 210 DDADSESENLSEALALLA---RKF----NRALRKIDRRTRPNVQDIKSDNSKSYNSQRKV 262
D A+ + + +SE L L A F R R+++ R V++ K + +R++
Sbjct: 273 DSANGDIDGISEFLELKALGEEPFPE*EERITREVELRRSDEVREAK*SFE---DEEREI 103
Query: 263 KEEDKSGQNKGVQ 275
+EEDK +N+G++
Sbjct: 102 EEEDKVEENRGIE 64
>TC91139 similar to GP|15292825|gb|AAK92781.1 unknown protein {Arabidopsis
thaliana}, partial (22%)
Length = 1108
Score = 28.1 bits (61), Expect = 4.5
Identities = 29/114 (25%), Positives = 49/114 (42%)
Frame = +3
Query: 26 AQAEGSTSTPEEKPEDELTSAEEEASLENSKAMNAIFNAVDKNMFRLINTCTVAKDAWEI 85
A EG TS P ++L+S E + + NSK+ AI + + F
Sbjct: 462 ALKEGGTSKP-----NDLSSKELQPLVANSKSKRAIEQLLMQETF--------------- 581
Query: 86 LKTTHEGTTRVRMSRLQMLTTRFENLTMTEDERISEYHMRVRDLSNASFALGEP 139
+ EG +++ R +++ + D+RI+ R RD+SN + A G P
Sbjct: 582 --SREEGDRLIKIIRSRVVDSP------ANDDRIT----RPRDISNMTLATGSP 707
>TC88387 similar to GP|13357253|gb|AAK20050.1 putative zinc finger protein
{Oryza sativa (japonica cultivar-group)}, partial (96%)
Length = 1286
Score = 27.7 bits (60), Expect = 5.9
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 276 CHECEGYGHIRSECAT 291
CH C GHI SEC+T
Sbjct: 481 CHNCSLPGHIASECST 528
>TC82467 similar to GP|9758269|dbj|BAB08768.1
emb|CAA18192.1~gene_id:MBG8.16~similar to unknown
protein {Arabidopsis thaliana}, partial (75%)
Length = 1543
Score = 27.7 bits (60), Expect = 5.9
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = -2
Query: 180 GSLQTYKLKLGEKPEKKTKSI 200
G LQTY +LG +PE+K +SI
Sbjct: 384 GPLQTYSGQLGAEPERKYRSI 322
>TC78556 similar to GP|9759255|dbj|BAB09690.1 contains similarity to unknown
protein~gb|AAF19567.1~gene_id:K2A11.1 {Arabidopsis
thaliana}, partial (46%)
Length = 1733
Score = 27.7 bits (60), Expect = 5.9
Identities = 11/43 (25%), Positives = 22/43 (50%)
Frame = +1
Query: 14 KAVVKGWKHPVKAQAEGSTSTPEEKPEDELTSAEEEASLENSK 56
+ ++ GWK V A + +T+TP E+ + + +EN +
Sbjct: 913 RTLIDGWKELVNAWVKATTTTPVAASEEGTPDSRNPSVVENEE 1041
>BF641303 similar to GP|9758269|dbj emb|CAA18192.1~gene_id:MBG8.16~similar to
unknown protein {Arabidopsis thaliana}, partial (39%)
Length = 681
Score = 27.7 bits (60), Expect = 5.9
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = -3
Query: 180 GSLQTYKLKLGEKPEKKTKSI 200
G LQTY +LG +PE+K +SI
Sbjct: 367 GPLQTYSGQLGAEPERKYRSI 305
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.126 0.341
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,985,181
Number of Sequences: 36976
Number of extensions: 67234
Number of successful extensions: 378
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 372
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 378
length of query: 310
length of database: 9,014,727
effective HSP length: 96
effective length of query: 214
effective length of database: 5,465,031
effective search space: 1169516634
effective search space used: 1169516634
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0101.12