BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0091a.6
(242 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87648 similar to GP|17529294|gb|AAL38874.1 unknown protein {Ar... 42 2e-04
TC76371 weakly similar to GP|23304737|emb|CAC87937. PDI-like pro... 39 0.002
TC76345 similar to GP|3928152|emb|CAA10290.1 ribulose 1 5-bispho... 39 0.002
TC78691 similar to GP|17380978|gb|AAL36301.1 unknown protein {Ar... 39 0.002
TC85449 homologue to PIR|S71923|S71923 cysteine proteinase (EC 3... 32 0.17
TC88456 similar to GP|15487292|dbj|BAB64531. vacuolar sorting re... 29 1.4
TC86219 similar to GP|17065520|gb|AAL32914.1 Unknown protein {Ar... 28 2.4
BI310451 weakly similar to GP|7339698|dbj| Similar to Caenorhabd... 28 4.2
AL367950 27 5.5
TC89451 weakly similar to GP|21536959|gb|AAM61300.1 unknown {Ara... 27 5.5
TC77708 weakly similar to GP|18266654|gb|AAL67600.1 unknown prot... 27 7.1
BQ148082 similar to PIR|S71923|S719 cysteine proteinase (EC 3.4.... 27 7.1
TC83848 similar to SP|P53393|SUT3_STYHA Low affinity sulphate tr... 27 7.1
TC86327 similar to GP|14335106|gb|AAK59832.1 At2g41900/T6D20.20 ... 27 7.1
TC81706 similar to PIR|T50672|T50672 probable zinc finger protei... 27 7.1
TC77833 similar to PIR|T09559|T09559 hypothetical protein L73G19... 27 7.1
TC83600 27 7.1
TC89814 similar to GP|15983392|gb|AAL11564.1 At1g59990/T2K10_4 {... 27 7.1
TC91323 similar to GP|18086336|gb|AAL57631.1 At1g78060/F28K19_32... 27 7.1
BE204827 similar to PIR|F86222|F86 hypothetical protein [importe... 27 9.3
>TC87648 similar to GP|17529294|gb|AAL38874.1 unknown protein {Arabidopsis
thaliana}, partial (33%)
Length = 990
Score = 42.4 bits (98), Expect = 2e-04
Identities = 18/50 (36%), Positives = 28/50 (56%)
Frame = +1
Query: 5 EISHFSHPQHKLRFEHNAFPFKCDGCKEIGIGSRYKCSICDYDLHMQCAI 54
+++H +H + N + CDGCK+ G Y C+ CD+DLH CA+
Sbjct: 586 KVTHETHEHELVLSRRNVYC--CDGCKDEGDTWSYLCAECDFDLHPNCAL 729
Score = 34.3 bits (77), Expect = 0.045
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +1
Query: 83 CNACEKDVNGFVYHCKSCGFDLHPCCA 109
C+ C+ + + + Y C C FDLHP CA
Sbjct: 646 CDGCKDEGDTWSYLCAECDFDLHPNCA 726
>TC76371 weakly similar to GP|23304737|emb|CAC87937. PDI-like protein {Quercus
suber}, partial (21%)
Length = 1710
Score = 38.5 bits (88), Expect = 0.002
Identities = 16/52 (30%), Positives = 24/52 (45%)
Frame = +3
Query: 57 PTLVHPFYTKCSFQFMSSPPGNIPRYCNACEKDVNGFVYHCKSCGFDLHPCC 108
P LVH +S G P C C++ + + Y C CG+++HP C
Sbjct: 1194 PNLVHHEGHHHGLNLVSDGNGGGPFICCVCDEQGSNWAYQCLQCGYEVHPKC 1349
Score = 31.2 bits (69), Expect = 0.38
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = +3
Query: 24 PFKCDGCKEIGIGSRYKCSICDYDLHMQC 52
PF C C E G Y+C C Y++H +C
Sbjct: 1263 PFICCVCDEQGSNWAYQCLQCGYEVHPKC 1349
>TC76345 similar to GP|3928152|emb|CAA10290.1 ribulose 1 5-bisphosphate
carboxylase small subunit {Cicer arietinum}, partial
(98%)
Length = 1243
Score = 38.5 bits (88), Expect = 0.002
Identities = 16/52 (30%), Positives = 24/52 (45%)
Frame = +1
Query: 57 PTLVHPFYTKCSFQFMSSPPGNIPRYCNACEKDVNGFVYHCKSCGFDLHPCC 108
P LVH +S G P C C++ + + Y C CG+++HP C
Sbjct: 946 PNLVHHEGHHHGLNLVSDGNGGGPFICCVCDEQGSNWAYQCLQCGYEVHPKC 1101
>TC78691 similar to GP|17380978|gb|AAL36301.1 unknown protein {Arabidopsis
thaliana}, partial (38%)
Length = 815
Score = 38.5 bits (88), Expect = 0.002
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +3
Query: 13 QHKLRFEHNAFPFKCDGCKEIGIGSRYKCSICDYDLHMQC 52
+H L+ + A + CD CK+ G + C +CDYDLH C
Sbjct: 369 EHLLKLDM-AKAYVCDSCKKQGKFWTFSCDVCDYDLHPSC 485
Score = 32.7 bits (73), Expect = 0.13
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +3
Query: 83 CNACEKDVNGFVYHCKSCGFDLHPCC 108
C++C+K + + C C +DLHP C
Sbjct: 408 CDSCKKQGKFWTFSCDVCDYDLHPSC 485
Score = 28.5 bits (62), Expect = 2.4
Identities = 8/30 (26%), Positives = 18/30 (59%)
Frame = +3
Query: 132 CHRCGRKGRSWSYRSKCKSYNLHVACVREM 161
C C ++G+ W++ Y+LH +C+ ++
Sbjct: 408 CDSCKKQGKFWTFSCDVCDYDLHPSCLEKV 497
>TC85449 homologue to PIR|S71923|S71923 cysteine proteinase (EC 3.4.22.-) -
garden pea, complete
Length = 1534
Score = 32.3 bits (72), Expect = 0.17
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = -3
Query: 49 HMQCAIPSPTLVHPFYTKCSFQFMSSPPGNIPRYCNACEKDVNGFVYHCK 98
H++C P+ + C FQF+ S G+I R KD+NG V+ CK
Sbjct: 881 HLKCHTNGPSK-----SNCMFQFILSTQGDIDR-----AKDLNGNVFRCK 762
>TC88456 similar to GP|15487292|dbj|BAB64531. vacuolar sorting receptor
{Vigna mungo}, partial (41%)
Length = 980
Score = 29.3 bits (64), Expect = 1.4
Identities = 14/38 (36%), Positives = 19/38 (49%)
Frame = -3
Query: 6 ISHFSHPQHKLRFEHNAFPFKCDGCKEIGIGSRYKCSI 43
+SHF P L F HN++ GC SR+ C+I
Sbjct: 786 LSHFG-PHSSLLFVHNSYSTLSSGCGRASRQSRFMCTI 676
>TC86219 similar to GP|17065520|gb|AAL32914.1 Unknown protein {Arabidopsis
thaliana}, complete
Length = 522
Score = 28.5 bits (62), Expect = 2.4
Identities = 14/43 (32%), Positives = 21/43 (48%)
Frame = +1
Query: 126 RKVSSPCHRCGRKGRSWSYRSKCKSYNLHVACVREMVVENWDH 168
R+ S CH+ SWSYR +C + + A + V+ W H
Sbjct: 139 RRHRSSCHQI----LSWSYRGRC*EFKIRSA--SQKVISEWRH 249
>BI310451 weakly similar to GP|7339698|dbj| Similar to Caenorhabditis elegans
chromosome V cosmid T10G3; Homo sapiens endosomal
protein P162, partial (10%)
Length = 755
Score = 27.7 bits (60), Expect = 4.2
Identities = 15/37 (40%), Positives = 22/37 (58%), Gaps = 4/37 (10%)
Frame = +3
Query: 95 YHCKSCGFDLHP-CCAKLPMVLDDGE---VKLYLYRK 127
YHC SC F L P C ++ M++ D E + L+L R+
Sbjct: 483 YHCLSCSFQL*PFCISRT*MLMSDYEGIDLSLFLERQ 593
>AL367950
Length = 317
Score = 27.3 bits (59), Expect = 5.5
Identities = 15/53 (28%), Positives = 22/53 (41%), Gaps = 1/53 (1%)
Frame = -2
Query: 104 LHPCCAK-LPMVLDDGEVKLYLYRKVSSPCHRCGRKGRSWSYRSKCKSYNLHV 155
LHP +P ++G + + +S C CG G Y YNLH+
Sbjct: 160 LHPDSKNWVPKEFEEGCIGVSTSHPISR*CFTCGGNGHIVRYMIAVNGYNLHL 2
>TC89451 weakly similar to GP|21536959|gb|AAM61300.1 unknown {Arabidopsis
thaliana}, partial (80%)
Length = 1609
Score = 27.3 bits (59), Expect = 5.5
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +3
Query: 90 VNGFVYHCKSCGFDLHPCCAKLP 112
+N V +CK+C + CC++LP
Sbjct: 447 LNNTVNYCKNCTIPICSCCSQLP 515
>TC77708 weakly similar to GP|18266654|gb|AAL67600.1 unknown protein {Oryza
sativa}, partial (30%)
Length = 1593
Score = 26.9 bits (58), Expect = 7.1
Identities = 7/28 (25%), Positives = 17/28 (60%)
Frame = +1
Query: 147 KCKSYNLHVACVREMVVENWDHVYVAGG 174
K K+Y +H++C++ ++ + +Y G
Sbjct: 43 KKKTYTIHISCIKVQIITQFSSLYKGSG 126
>BQ148082 similar to PIR|S71923|S719 cysteine proteinase (EC 3.4.22.-) -
garden pea, partial (22%)
Length = 664
Score = 26.9 bits (58), Expect = 7.1
Identities = 14/30 (46%), Positives = 18/30 (59%)
Frame = -2
Query: 69 FQFMSSPPGNIPRYCNACEKDVNGFVYHCK 98
FQF+ S G+I R KD+NG V+ CK
Sbjct: 663 FQFILSTQGDIDR-----AKDLNGNVFRCK 589
>TC83848 similar to SP|P53393|SUT3_STYHA Low affinity sulphate transporter
3. {Stylosanthes hamata}, partial (21%)
Length = 769
Score = 26.9 bits (58), Expect = 7.1
Identities = 14/43 (32%), Positives = 25/43 (57%), Gaps = 4/43 (9%)
Frame = -3
Query: 110 KLPMVLDDGEVKLYLYRKVSSPCHRCGRKG----RSWSYRSKC 148
K+P+ DG+ K+ + ++S+ + G +G R +SYRS C
Sbjct: 674 KIPVKKVDGKDKISIGMRISNRIYDIGDQGR*KHRHYSYRSNC 546
>TC86327 similar to GP|14335106|gb|AAK59832.1 At2g41900/T6D20.20 {Arabidopsis
thaliana}, partial (50%)
Length = 3018
Score = 26.9 bits (58), Expect = 7.1
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 181 VETSVPGLKNTLYAAHNSRRSKGKVKKC 208
V+ S+P +KN++YA R KV+ C
Sbjct: 992 VDPSLPDIKNSMYATDEFRMYSFKVRPC 1075
>TC81706 similar to PIR|T50672|T50672 probable zinc finger protein
[imported] - Arabidopsis thaliana, partial (67%)
Length = 780
Score = 26.9 bits (58), Expect = 7.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3
Query: 11 HPQHKLRFEHNAFPFKCDGCKEI 33
H QH + + PF CDGCK++
Sbjct: 99 HCQHVDCHQLDFLPFTCDGCKQV 167
>TC77833 similar to PIR|T09559|T09559 hypothetical protein L73G19.50 -
Arabidopsis thaliana, partial (49%)
Length = 1472
Score = 26.9 bits (58), Expect = 7.1
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 122 LYLYRKVSSPCHRCGRKGRSWSYRSKCKSYN 152
+YL+R V C R + R SY+ C SY+
Sbjct: 994 VYLFRTVRKQCTRTTLRSRMGSYKILC*SYS 1086
>TC83600
Length = 293
Score = 26.9 bits (58), Expect = 7.1
Identities = 12/23 (52%), Positives = 14/23 (60%), Gaps = 5/23 (21%)
Frame = -3
Query: 67 CSFQFMSSPPGN-----IPRYCN 84
CSF F SSPPGN P+ C+
Sbjct: 282 CSFYFHSSPPGNKVYKFFPKICD 214
>TC89814 similar to GP|15983392|gb|AAL11564.1 At1g59990/T2K10_4 {Arabidopsis
thaliana}, partial (14%)
Length = 671
Score = 26.9 bits (58), Expect = 7.1
Identities = 19/62 (30%), Positives = 27/62 (42%), Gaps = 9/62 (14%)
Frame = -1
Query: 54 IPSPTLVHPFYTKCS-------FQFMSSPPGNIPRYCNAC--EKDVNGFVYHCKSCGFDL 104
+P+ T+ H + F + P NIP Y +A E+ N YHC+S L
Sbjct: 596 LPAHTIAHSDKARAELSWPAERFLYQIQHPENIPLYPSAYQ*EEVDNCMSYHCRSLQQ*L 417
Query: 105 HP 106
HP
Sbjct: 416 HP 411
>TC91323 similar to GP|18086336|gb|AAL57631.1 At1g78060/F28K19_32
{Arabidopsis thaliana}, partial (25%)
Length = 720
Score = 26.9 bits (58), Expect = 7.1
Identities = 14/41 (34%), Positives = 18/41 (43%), Gaps = 4/41 (9%)
Frame = -2
Query: 61 HPFYTKCSFQFMSSPPGNIPRY----CNACEKDVNGFVYHC 97
H +T C F+SSPP IP Y C C + +C
Sbjct: 566 HRIFTSC---FLSSPPSWIPEYVYVWCPKCHPHCLSSIVYC 453
>BE204827 similar to PIR|F86222|F86 hypothetical protein [imported] -
Arabidopsis thaliana, partial (18%)
Length = 626
Score = 26.6 bits (57), Expect = 9.3
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = +3
Query: 83 CNACEKDVNGFVYHCKSCGFDLHPCCAK 110
CN C + + C SC +DL C +
Sbjct: 336 CNLCRIPITDYHRRCPSCSYDLCLICCR 419
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.138 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,988,773
Number of Sequences: 36976
Number of extensions: 218056
Number of successful extensions: 1380
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 1353
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1379
length of query: 242
length of database: 9,014,727
effective HSP length: 93
effective length of query: 149
effective length of database: 5,575,959
effective search space: 830817891
effective search space used: 830817891
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0091a.6