BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0082b.8
(726 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90892 similar to GP|7940285|gb|AAF70844.1| F24O1.19 {Arabidops... 286 1e-77
AW689612 weakly similar to GP|19698963|gb| unknown protein {Arab... 239 2e-63
TC92651 similar to GP|4204288|gb|AAD10669.1| lcl|prt_seq No defi... 197 8e-56
BG585502 similar to GP|7268595|em predicted protein of unknown f... 52 1e-06
TC78227 similar to GP|10176883|dbj|BAB10113. contains similarity... 44 3e-04
BF004323 similar to GP|4337011|gb| zinc-binding peroxisomal inte... 42 0.001
TC81849 similar to GP|19698885|gb|AAL91178.1 putative protein {A... 41 0.002
TC80776 similar to GP|13605815|gb|AAK32893.1 At2g32700/F24L7.16 ... 40 0.002
TC88730 weakly similar to GP|21592748|gb|AAM64697.1 PRT1 {Arabid... 40 0.002
TC93528 similar to GP|20466490|gb|AAM20562.1 unknown protein {Ar... 40 0.004
AJ501352 similar to PIR|B84584|B845 probable RING zinc finger pr... 39 0.007
TC91998 39 0.007
TC82517 homologue to GP|7688065|emb|CAB89694.1 constitutively ph... 38 0.016
TC86451 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsi... 37 0.021
TC86450 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsi... 37 0.021
TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protei... 37 0.027
TC89819 weakly similar to GP|22795037|gb|AAN05420.1 putative RIN... 37 0.027
TC91938 similar to PIR|E96612|E96612 probable transcription fact... 37 0.027
TC89577 similar to GP|22795037|gb|AAN05420.1 putative RING prote... 37 0.035
TC79724 similar to GP|22212705|gb|AAM94371.1 CTD phosphatase-lik... 37 0.035
>TC90892 similar to GP|7940285|gb|AAF70844.1| F24O1.19 {Arabidopsis
thaliana}, partial (12%)
Length = 803
Score = 286 bits (733), Expect = 1e-77
Identities = 146/190 (76%), Positives = 159/190 (82%)
Frame = +3
Query: 536 TSDVTHVIAATDANGACSRTLKVLMAILNGRWVLKIDWIKACMKVMSLVEEEPYEISLDN 595
TSDVTHVIAATDANGACSRTLKVL AILNG+W+LK+DWI+ACMK M+LVEEE YEI LDN
Sbjct: 3 TSDVTHVIAATDANGACSRTLKVLRAILNGQWILKMDWIRACMKAMNLVEEELYEIDLDN 182
Query: 596 QGCNGGPKAGRLSALANEPKLFSGLKFYFSGDYVSSYKEDLEDLVEVGGGAVLTSKDKLE 655
QGC GGPKAGRL ALANEPKLFSGLKFYFSG+Y SSYK+ LEDLVE GGG VL SKD+LE
Sbjct: 183 QGCQGGPKAGRLRALANEPKLFSGLKFYFSGEYDSSYKKYLEDLVEGGGGVVLKSKDELE 362
Query: 656 AKRHEFEVTSNLLIVYNLDPPQGSKLGDEVSTLWQRLNEAEDLAANTGSQVIGHTWILES 715
R +NLL VYNLDPP+G +L DEVS LW RL EAE+L ANT GHTWILES
Sbjct: 363 DGR-----DANLLAVYNLDPPEGCELEDEVSILWHRLTEAENLTANTA----GHTWILES 515
Query: 716 IAACKLQPFI 725
IAACKLQPF+
Sbjct: 516 IAACKLQPFV 545
Score = 32.7 bits (73), Expect = 0.51
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +1
Query: 703 GSQVIGHTWILESIAACKLQPFI 725
G +GH WILESI+A KL F+
Sbjct: 595 GCSTVGHMWILESISAFKLHAFV 663
>AW689612 weakly similar to GP|19698963|gb| unknown protein {Arabidopsis
thaliana}, partial (2%)
Length = 653
Score = 239 bits (611), Expect = 2e-63
Identities = 125/220 (56%), Positives = 157/220 (70%), Gaps = 2/220 (0%)
Frame = +1
Query: 1 MEDSGKP--NNKSKLMNPWMLHFQKLALELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSS 58
ME+SGK NN +KL+NPWMLHFQKLALELKCPLCL+LF+KPVLLPC+HLFC SCL DS+
Sbjct: 37 MENSGKSIMNNNTKLLNPWMLHFQKLALELKCPLCLNLFKKPVLLPCNHLFCSSCLADST 216
Query: 59 LSGSECAVCKTKYAQTDIRNVPFVENMVAIYRSLDASFCASMLQQRSNDDSRVLQQCQTF 118
SECA+C TK +Q DIR++PFVE++V IY+SLDA+FCA+ QQRS+D++RVL+QCQT
Sbjct: 217 SIRSECALCNTKCSQKDIRHLPFVESIVTIYKSLDATFCANAFQQRSSDNTRVLEQCQTL 396
Query: 119 RDSSYSNIKKADNFSQSSPNSNGFGVGENRKSMITMHVKPEELEMSSGGRAGFRNDVKPY 178
+S++SN K D Q+S SN GVG+N +S I M+ K +E+EMS A KP
Sbjct: 397 HNSAFSN--KVDKVLQNSCMSNEVGVGKNHQSGIKMNGKAKEVEMSCKRXADNHIAEKP- 567
Query: 179 PMQRSRVEIGDYVEMDVNQVTQAAVYSPPFCDTKGSDNDC 218
M++NQ TQ+ SPPFCDTK SDN C
Sbjct: 568 ------------DSMNLNQATQSEPDSPPFCDTKXSDNGC 651
>TC92651 similar to GP|4204288|gb|AAD10669.1| lcl|prt_seq No definition line
found {Arabidopsis thaliana}, partial (5%)
Length = 554
Score = 197 bits (501), Expect(2) = 8e-56
Identities = 92/134 (68%), Positives = 111/134 (82%)
Frame = +2
Query: 268 RDLKRKKYLTKGDDHIQHVSTHHSKLVDSHCGLDLKSGKEPGELLPANIPIDLNPSTSIC 327
RDLKR+K LT D +Q +++H+KLVDS C L++KS K+ G L+P+N P DL PSTSIC
Sbjct: 152 RDLKRQKNLTNEDGIVQQSTSYHNKLVDSRCDLEIKSDKDTGALVPSNAPSDLYPSTSIC 331
Query: 328 SFCQSSETSEATGPMLHYANGKSVIGDAAMQPNVIHVHRCCVDWAPQVYFVDETCKNLKA 387
SFCQSS+ SEATG MLHYA+G SV G+AAM+PNV+HVH+ C+DWAPQVYFV ET KNLKA
Sbjct: 332 SFCQSSKISEATGSMLHYASGISVTGEAAMEPNVVHVHKVCIDWAPQVYFVGETVKNLKA 511
Query: 388 EVARGAKLKCSTCG 401
EVARGAKLKC+ CG
Sbjct: 512 EVARGAKLKCTKCG 553
Score = 38.9 bits (89), Expect(2) = 8e-56
Identities = 23/37 (62%), Positives = 26/37 (70%), Gaps = 1/37 (2%)
Frame = +1
Query: 232 LENSSLKRAST-GKGNLKERKSQFRSESSASETDKPT 267
L+ S KR+ST GKGNLKER F SESSASE + T
Sbjct: 40 LKVRSFKRSSTTGKGNLKERMPHFWSESSASENEDLT 150
>BG585502 similar to GP|7268595|em predicted protein of unknown function
{Arabidopsis thaliana}, partial (13%)
Length = 845
Score = 51.6 bits (122), Expect = 1e-06
Identities = 36/121 (29%), Positives = 53/121 (43%), Gaps = 5/121 (4%)
Frame = +3
Query: 535 WTSDVTHVIAATDANGACSRTLKVLMAILNGRWVLKIDWIKACMKVMSLVEEEPYE---- 590
W+ TH IA RT K A +GRW+LK D++ A + L+ EEPYE
Sbjct: 267 WSYQATHFIAPDPLR----RTEKFFAATASGRWILKTDFLSASSQAGKLLPEEPYEWHKN 434
Query: 591 -ISLDNQGCNGGPKAGRLSALANEPKLFSGLKFYFSGDYVSSYKEDLEDLVEVGGGAVLT 649
+S D P+ RL F G++ GD + + L+ V+ G G +L
Sbjct: 435 GLSEDGAINMEAPRKWRLLKERTGHGAFYGMRIVVYGDCFAPPLDTLKRAVKAGDGTILA 614
Query: 650 S 650
+
Sbjct: 615 T 617
>TC78227 similar to GP|10176883|dbj|BAB10113. contains similarity to unknown
protein~gb|AAD10691.1~gene_id:K9L2.3 {Arabidopsis
thaliana}, partial (27%)
Length = 1707
Score = 43.5 bits (101), Expect = 3e-04
Identities = 24/71 (33%), Positives = 40/71 (55%), Gaps = 4/71 (5%)
Frame = +1
Query: 27 ELKCPLCLSLFEKP-VLLPCDHLFCDSCLVDS-SLSGSECAVCKTKYA-QTDIRNVPFVE 83
E++CP+CL + K ++ C H FC C+ S L +EC C+T A + +R+ P +
Sbjct: 316 EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 495
Query: 84 NMV-AIYRSLD 93
++ AIY +D
Sbjct: 496 ALIAAIYPDID 528
>BF004323 similar to GP|4337011|gb| zinc-binding peroxisomal integral
membrane protein {Arabidopsis thaliana}, partial (32%)
Length = 513
Score = 41.6 bits (96), Expect = 0.001
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = -1
Query: 29 KCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDI 76
KC LCLS + P C H+FC +C+ + EC +C+T + +
Sbjct: 258 KCTLCLSNRQHPTATSCGHVFCWNCITEWCNEKPECPLCRTPITHSSL 115
>TC81849 similar to GP|19698885|gb|AAL91178.1 putative protein {Arabidopsis
thaliana}, partial (16%)
Length = 1477
Score = 40.8 bits (94), Expect = 0.002
Identities = 20/50 (40%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Frame = +1
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGS---ECAVCKTKYAQTDI 76
C +CL + PVL C HLFC C S + S EC VCK + ++ I
Sbjct: 160 CNICLDIARDPVLTCCGHLFCWPCFYQLSYAYSKAKECPVCKGEVTESGI 309
>TC80776 similar to GP|13605815|gb|AAK32893.1 At2g32700/F24L7.16
{Arabidopsis thaliana}, partial (19%)
Length = 1087
Score = 40.4 bits (93), Expect = 0.002
Identities = 19/55 (34%), Positives = 32/55 (57%), Gaps = 3/55 (5%)
Frame = +2
Query: 20 HFQKLALELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSG---SECAVCKTKY 71
H +++ C +CL LF KP++L C ++ C C + S+SG S+C C+ +Y
Sbjct: 77 HEEEIPDSFCCCVCLDLFYKPIVLSCGYMCCFWC-IHKSMSGVRESKCPTCRHQY 238
>TC88730 weakly similar to GP|21592748|gb|AAM64697.1 PRT1 {Arabidopsis
thaliana}, partial (28%)
Length = 802
Score = 40.4 bits (93), Expect = 0.002
Identities = 19/55 (34%), Positives = 31/55 (55%), Gaps = 3/55 (5%)
Frame = +2
Query: 20 HFQKLALELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSG---SECAVCKTKY 71
H +++ C +CL L KP++L C H+ C C + S+SG S+C C+ +Y
Sbjct: 113 HEEEIPDSFCCCVCLDLLYKPIVLSCGHMCCFWC-IHKSMSGVRESKCPTCRHQY 274
Score = 38.1 bits (87), Expect = 0.012
Identities = 15/48 (31%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +2
Query: 22 QKLAL-ELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCK 68
QK+++ ++ C +C L PV+L C H++C++C+ + C VC+
Sbjct: 611 QKISVADVMCTMCKQLLFHPVVLHCGHVYCETCVYKLADEMLRCQVCQ 754
>TC93528 similar to GP|20466490|gb|AAM20562.1 unknown protein {Arabidopsis
thaliana}, partial (24%)
Length = 755
Score = 39.7 bits (91), Expect = 0.004
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +3
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIR 77
C +C P+LL C H+FC+ C+ + C +C+ D+R
Sbjct: 168 CAICQEKMHSPILLRCKHIFCEDCVSEWFERERTCPLCRALVKAADLR 311
>AJ501352 similar to PIR|B84584|B845 probable RING zinc finger protein
[imported] - Arabidopsis thaliana, partial (17%)
Length = 607
Score = 38.9 bits (89), Expect = 0.007
Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 4/58 (6%)
Frame = +3
Query: 28 LKCPLCLSLFEKPVLL----PCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIRNVPF 81
L+C +CLS FE +L C H F +C+ S S C +C+ ++DIRN F
Sbjct: 405 LECTVCLSKFEDEEILRLLPKCKHAFHMNCIDKWLESHSTCPLCRYMVEESDIRNFTF 578
>TC91998
Length = 403
Score = 38.9 bits (89), Expect = 0.007
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = +2
Query: 690 QRLNEAEDLAANTGSQVIGHTWILESIAACKLQ 722
QR +AE LA T S+V +TWIL SIA CKLQ
Sbjct: 38 QRRCDAEALARPTCSKVASNTWILNSIAGCKLQ 136
>TC82517 homologue to GP|7688065|emb|CAB89694.1 constitutively
photomorphogenic 1 protein {Pisum sativum}, partial
(22%)
Length = 671
Score = 37.7 bits (86), Expect = 0.016
Identities = 13/45 (28%), Positives = 23/45 (50%)
Frame = +3
Query: 23 KLALELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVC 67
+L + CP+C+ + + L C H FC C++ + S+C C
Sbjct: 138 ELDKDFLCPICMQIIKDAFLTSCGHSFCYMCIITHLRNKSDCPCC 272
>TC86451 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsis
thaliana}, partial (51%)
Length = 995
Score = 37.4 bits (85), Expect = 0.021
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = +2
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIRNV 79
CP+C+ + + C H+FC SC+ + + ++C C+ K ++ V
Sbjct: 653 CPICMGPMVEEMSTRCGHIFCKSCIKAAISAQAKCPTCRKKITVKELIRV 802
>TC86450 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsis
thaliana}, partial (39%)
Length = 1268
Score = 37.4 bits (85), Expect = 0.021
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = +3
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIRNV 79
CP+C+ + + C H+FC SC+ + + ++C C+ K ++ V
Sbjct: 741 CPICMGPMVEEMSTRCGHIFCKSCIKAAISAQAKCPTCRKKITVKELIRV 890
>TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protein~similar
to Arabidopsis thaliana chromosome 1 F18O14.3, partial
(23%)
Length = 978
Score = 37.0 bits (84), Expect = 0.027
Identities = 16/43 (37%), Positives = 22/43 (50%), Gaps = 3/43 (6%)
Frame = +2
Query: 29 KCPLCLSLFEKPVLLPCDHLFCDSCL---VDSSLSGSECAVCK 68
+C +C L + P++ C HLFC CL + EC VCK
Sbjct: 143 ECNICFDLAQDPIITLCGHLFCWPCLYKWLHFHSQSRECPVCK 271
>TC89819 weakly similar to GP|22795037|gb|AAN05420.1 putative RING protein
{Populus x canescens}, partial (33%)
Length = 880
Score = 37.0 bits (84), Expect = 0.027
Identities = 17/58 (29%), Positives = 30/58 (51%), Gaps = 11/58 (18%)
Frame = +2
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCL-----VDSSLSGS------ECAVCKTKYAQTDI 76
C +CL + PV+ C HL+C C+ + S +S +C VCK++ +Q+ +
Sbjct: 191 CNICLDCVQDPVVTFCGHLYCWPCIYKWLDIQSGISSENEKQKPQCPVCKSELSQSSL 364
>TC91938 similar to PIR|E96612|E96612 probable transcription factor
F12K22.14 [imported] - Arabidopsis thaliana, partial
(11%)
Length = 685
Score = 37.0 bits (84), Expect = 0.027
Identities = 17/43 (39%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = +3
Query: 28 LKCPLCLSLFEKPVLLPCDHLFCDSCLVD-SSLSGSECAVCKT 69
L C C+ L E+PV PC H FC C L C+ C+T
Sbjct: 441 LNCSFCVKLPERPVTTPCGHNFCLKCFEKWVGLGKRTCSNCRT 569
>TC89577 similar to GP|22795037|gb|AAN05420.1 putative RING protein {Populus
x canescens}, partial (56%)
Length = 954
Score = 36.6 bits (83), Expect = 0.035
Identities = 19/55 (34%), Positives = 25/55 (44%), Gaps = 10/55 (18%)
Frame = +1
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLV-------DSSLSGSE---CAVCKTKYAQT 74
C +CL +PV+ C HL+C C+ D SL E C VCK + T
Sbjct: 205 CNICLDFANEPVVTLCGHLYCWPCIYKWLHVQSDDSLGSDEHPQCPVCKENISHT 369
>TC79724 similar to GP|22212705|gb|AAM94371.1 CTD phosphatase-like 3
{Arabidopsis thaliana}, partial (11%)
Length = 1952
Score = 36.6 bits (83), Expect = 0.035
Identities = 23/71 (32%), Positives = 35/71 (48%)
Frame = +1
Query: 524 ASKVGATVTKFWTSDVTHVIAATDANGACSRTLKVLMAILNGRWVLKIDWIKACMKVMSL 583
A + GA+ T VTHV+A + T KV AI NG++V+ W++A +
Sbjct: 244 AEQFGASCTNQIDDQVTHVVAHSPG------TDKVNWAIANGKFVVHPGWVEASALLYRR 405
Query: 584 VEEEPYEISLD 594
E+ + I LD
Sbjct: 406 ANEQDFAIKLD 438
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.132 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,820,767
Number of Sequences: 36976
Number of extensions: 378859
Number of successful extensions: 2115
Number of sequences better than 10.0: 110
Number of HSP's better than 10.0 without gapping: 2072
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2107
length of query: 726
length of database: 9,014,727
effective HSP length: 103
effective length of query: 623
effective length of database: 5,206,199
effective search space: 3243461977
effective search space used: 3243461977
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0082b.8