TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0074.3
         (267 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC87529 similar to GP|15293029|gb|AAK93625.1 unknown protein {Ar...   324  2e-89
BE239650 similar to PIR|D87615|D876 conserved hypothetical prote...   129  1e-30
TC87530                                                               118  2e-27
BG454377 similar to PIR|A85055|A85 probable leucyl tRNA syntheta...    28  4.8
TC88030 similar to PIR|F86416|F86416 probable RNA-binding protei...    27  8.2

>TC87529 similar to GP|15293029|gb|AAK93625.1 unknown protein {Arabidopsis
           thaliana}, partial (54%)
          Length = 687

 Score =  324 bits (831), Expect = 2e-89
 Identities = 154/207 (74%), Positives = 182/207 (87%)
 Frame = +1

Query: 1   MALKDTFYISHGSPTLSIDESLVARKFLQSWKKEVFPPRPTSILVISGHWDTAVPTVNVV 60
           MALK+TFYISHGSPTL+IDE++ A KFL SWK EVFP RP++ILVISGHWDT+VPTVNVV
Sbjct: 73  MALKETFYISHGSPTLAIDETIPAWKFLTSWK-EVFPERPSAILVISGHWDTSVPTVNVV 249

Query: 61  DSTNDTIYDFYGFPKPMYQLKYPAPGAPHLAKRVKELLKEGGFSRVDEDKKRGLDHGAWV 120
           +  N+TI+DF GFP+ MY+LKYPAPGAP LAKRVKEL++  G SRVDEDKKRGLDHG WV
Sbjct: 250 NH-NETIHDFGGFPRSMYKLKYPAPGAPKLAKRVKELIEASGLSRVDEDKKRGLDHGTWV 426

Query: 121 PLLLMYPEADIPVCQLSVQSNLDGTHHYNIGKALAPLKDEGVLIVGSGSAVHNLRALERH 180
           PL+LMYPEADIPVCQLSV SN +GT+HYN+GKA+APLKDEGVLI+GSGSA HN+RA+   
Sbjct: 427 PLMLMYPEADIPVCQLSVSSNRNGTYHYNLGKAIAPLKDEGVLIIGSGSATHNMRAIGPR 606

Query: 181 ATVAAPWAVEFDNWLKEALLEGRYEDV 207
            +   PWA+ FD+WLKE+L+EGRYED+
Sbjct: 607 ESPPPPWALAFDSWLKESLVEGRYEDI 687


>BE239650 similar to PIR|D87615|D876 conserved hypothetical protein CC2958
           [imported] - Caulobacter crescentus, partial (10%)
          Length = 628

 Score =  129 bits (324), Expect = 1e-30
 Identities = 74/203 (36%), Positives = 113/203 (55%), Gaps = 6/203 (2%)
 Frame = +3

Query: 39  RPTSILVISGHWDTAVPTVNVVDST--NDTIYDFYGFPKPMYQLKYPAPGAPHLAKRVKE 96
           +P +IL++S HW T    + V      N+  +D+ GFPK MY+L+Y APG+P LAK+V  
Sbjct: 45  KPKAILIVSAHWITNNKILKVTSKKQHNELYFDYGGFPKEMYELQYNAPGSPELAKKVIG 224

Query: 97  LLKEGGFSRVDEDKKRGLDHGAWVPLLLMYPEADIPVCQLSVQSNLDGTHHYNIGKALAP 156
           LL + G  + +ED KR  DH  + PL+ ++P  DIP+ +++   N     H  +GKAL  
Sbjct: 225 LLSDAGI-KAEEDTKRNFDH*VFGPLMEIWPSTDIPIVEINFD*NY*SKFHIPVGKALPS 401

Query: 157 LKDEGVLIVGSGSAVHN----LRALERHATVAAPWAVEFDNWLKEALLEGRYEDVNHYEQ 212
            ++EG+ I+GSG   H+    +   +  AT   P  +E DN      +E R + +  +E 
Sbjct: 402 FREEGIFIIGSGEITHHFSGGITGAKTFATAINP-VIENDN------IEERNKGLIEWE- 557

Query: 213 KAPHAKKAHPWPDHFYPLHVAIG 235
           K   A+++H   DH  PLHVA G
Sbjct: 558 KLNGARESHAQEDHLIPLHVAAG 626


>TC87530 
          Length = 699

 Score =  118 bits (296), Expect = 2e-27
 Identities = 58/80 (72%), Positives = 71/80 (88%)
 Frame = +3

Query: 1   MALKDTFYISHGSPTLSIDESLVARKFLQSWKKEVFPPRPTSILVISGHWDTAVPTVNVV 60
           MALK+TFYISHGSPTL+IDE++ A KFL SW KEVFP RP++ILVISGHWDT+VPTVNVV
Sbjct: 48  MALKETFYISHGSPTLAIDETIPAWKFLTSW-KEVFPERPSAILVISGHWDTSVPTVNVV 224

Query: 61  DSTNDTIYDFYGFPKPMYQL 80
           +  N+TI+DF GFP+ MY++
Sbjct: 225 NH-NETIHDFGGFPRSMYKV 281


>BG454377 similar to PIR|A85055|A85 probable leucyl tRNA synthetase
           [imported] - Arabidopsis thaliana, partial (11%)
          Length = 670

 Score = 27.7 bits (60), Expect = 4.8
 Identities = 15/35 (42%), Positives = 19/35 (53%)
 Frame = -1

Query: 223 WPDHFYPLHVAIGAAGENSKAKLIHSSIDLGSLSY 257
           WP   +      GA G+NSK K   S+ DLG LS+
Sbjct: 181 WPRRDHSSSAMCGAYGDNSKTKA--STTDLGCLSH 83


>TC88030 similar to PIR|F86416|F86416 probable RNA-binding protein MEI2
           36123-32976 [imported] - Arabidopsis thaliana, partial
           (23%)
          Length = 1549

 Score = 26.9 bits (58), Expect = 8.2
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +3

Query: 49  HWDTAVPTVNVVDSTNDTIYDFYGFPKPMY 78
           HW     TVNV DS   T+++ YG  + +Y
Sbjct: 900 HWLFETSTVNVEDSELRTLFEQYGDIRTLY 989


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.317    0.136    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,906,249
Number of Sequences: 36976
Number of extensions: 131041
Number of successful extensions: 663
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 644
length of query: 267
length of database: 9,014,727
effective HSP length: 94
effective length of query: 173
effective length of database: 5,538,983
effective search space: 958244059
effective search space used: 958244059
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)