BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0072a.1
(224 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82114 154 1e-45
BQ139915 similar to GP|21537031|gb| putative replication factor ... 139 1e-33
BG457283 weakly similar to PIR|B86206|B862 protein F10K1.17 [imp... 37 0.006
TC86923 similar to SP|Q9SEC2|MSRA_LACSA Peptide methionine sulfo... 28 2.8
TC86922 similar to SP|Q9SEC2|MSRA_LACSA Peptide methionine sulfo... 28 2.8
TC92156 similar to GP|15724296|gb|AAL06541.1 AT5g61660/k11j9_180... 27 4.8
TC87241 weakly similar to PIR|B71408|B71408 probable acylaminoac... 27 4.8
TC86232 similar to GP|21593313|gb|AAM65262.1 protein disulfide i... 27 8.2
BF650479 homologue to PIR|T06643|T066 hypothetical protein T20K1... 27 8.2
>TC82114
Length = 770
Score = 154 bits (388), Expect(3) = 1e-45
Identities = 73/99 (73%), Positives = 86/99 (86%)
Frame = +3
Query: 126 KSFQGVRQVAAFSVRPVVNFDEIPFHFIDCIHHHLRSKLKMEGTISTDPPASDSSLKTPV 185
K+FQGVRQ+ AFSVRPV NFDEIPFHFIDCIH+HLRSKLK+EG I++ P+S+SS+ TPV
Sbjct: 96 KAFQGVRQLVAFSVRPVTNFDEIPFHFIDCIHNHLRSKLKIEG-ITSALPSSNSSMNTPV 272
Query: 186 RNTSNGSQAPSSTPAYAQYGVDGLKDCDKLVIDYLQQHS 224
RN SNGSQAPSS P Y QY DGLKD DKLV++YL++HS
Sbjct: 273 RNVSNGSQAPSSNPGYVQYSTDGLKDIDKLVLNYLEKHS 389
Score = 35.8 bits (81), Expect(3) = 1e-45
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = +2
Query: 108 QEVEEIMNGMYVRVNGHLKSF 128
+E+EE++NGMYVRV G LKSF
Sbjct: 41 KEMEEVLNGMYVRVYGSLKSF 103
Score = 30.8 bits (68), Expect(3) = 1e-45
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = +1
Query: 95 IKCRRWINDAFDTQ 108
IKCRRW+ND FD++
Sbjct: 1 IKCRRWVNDTFDSK 42
>BQ139915 similar to GP|21537031|gb| putative replication factor A
{Arabidopsis thaliana}, partial (13%)
Length = 627
Score = 139 bits (349), Expect = 1e-33
Identities = 74/155 (47%), Positives = 103/155 (65%), Gaps = 7/155 (4%)
Frame = +3
Query: 6 QFDSSSG--GGGFTSTQLN---DSSPAPQKGRESQGLVPVTVKQINEASQSGDEKSSFVI 60
QFD ++ GGGF +Q N +SS P K RESQ L+P+T+KQIN A QS D+++ I
Sbjct: 147 QFDGNAAFAGGGFMPSQTNQGGESSLTPSKNRESQTLLPLTIKQINHALQSSDDRTGLTI 326
Query: 61 NGVELTNVTLVGMVFEKAERNTDVNFVLDDGTGRIKCRRWINDAFDTQEVEEIMNGMYVR 120
+GV + VTLVG V K+ + T+ FVLDDGTG I+C +W+++ D VE I+NGMYVR
Sbjct: 327 DGVGVNTVTLVGRVCNKSGQITEFKFVLDDGTGTIECTKWLHEPADAMAVESILNGMYVR 506
Query: 121 VNGHLKSFQGVRQVAAFSVRPV--VNFDEIPFHFI 153
+ G LK FQG + ++ FS+RP + D +PFH +
Sbjct: 507 LYGQLKGFQG-KTLSIFSLRPRY*LQ*DRLPFHXV 608
Score = 29.6 bits (65), Expect = 0.97
Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = +1
Query: 123 GHLKSFQGVRQVA-AFSVR-PVVNFDEIPFHFIDCIHHHL 160
G++ S +G R FS+ PV +F+EI HF +CI+ HL
Sbjct: 505 GYMDS*KGFRVKP*VFSLSGPVTDFNEIASHFXECIYVHL 624
>BG457283 weakly similar to PIR|B86206|B862 protein F10K1.17 [imported] -
Arabidopsis thaliana, partial (25%)
Length = 664
Score = 37.0 bits (84), Expect = 0.006
Identities = 26/121 (21%), Positives = 47/121 (38%), Gaps = 20/121 (16%)
Frame = +3
Query: 57 SFVINGVELTNVTLVGMVFEKAERNTD-VNFVLDDGTGRIKCRRWINDAFD--------- 106
SF N + +T VG + + + + F +DDGTG I C W+N
Sbjct: 117 SFTRNSISITRAETVGTITLRDHKPFKFLRFAIDDGTGCIPCILWLNHMTSPHLARRRSP 296
Query: 107 ----------TQEVEEIMNGMYVRVNGHLKSFQGVRQVAAFSVRPVVNFDEIPFHFIDCI 156
+ E + G RV G + ++G Q+ V + + H+++C+
Sbjct: 297 QDLCLLADAAARSAEVVKFGNVARVRGRVTEYKGGVQITVTDVVSERDPNVEVLHWVECV 476
Query: 157 H 157
+
Sbjct: 477 N 479
>TC86923 similar to SP|Q9SEC2|MSRA_LACSA Peptide methionine sulfoxide
reductase (EC 1.8.4.6) (Protein- methionine-S-oxide
reductase), partial (55%)
Length = 822
Score = 28.1 bits (61), Expect = 2.8
Identities = 22/65 (33%), Positives = 29/65 (43%), Gaps = 9/65 (13%)
Frame = -3
Query: 9 SSSGGGGFTSTQLNDSSPAPQKG---RESQGLVPVTVKQINEASQS------GDEKSSFV 59
SSSG G + L+D P P R +GLV V ++ E Q E +FV
Sbjct: 418 SSSGP*GMVESMLSDDLPLPNPSLFRRFMEGLVTVKWGRVGENKQECLTENLESEGRNFV 239
Query: 60 INGVE 64
+ GVE
Sbjct: 238 LAGVE 224
>TC86922 similar to SP|Q9SEC2|MSRA_LACSA Peptide methionine sulfoxide
reductase (EC 1.8.4.6) (Protein- methionine-S-oxide
reductase), partial (70%)
Length = 1207
Score = 28.1 bits (61), Expect = 2.8
Identities = 22/65 (33%), Positives = 29/65 (43%), Gaps = 9/65 (13%)
Frame = -2
Query: 9 SSSGGGGFTSTQLNDSSPAPQKG---RESQGLVPVTVKQINEASQS------GDEKSSFV 59
SSSG G + L+D P P R +GLV V ++ E Q E +FV
Sbjct: 360 SSSGP*GMVESMLSDDLPLPNPSLFRRFMEGLVTVKWGRVGENKQECLTENLESEGRNFV 181
Query: 60 INGVE 64
+ GVE
Sbjct: 180 LAGVE 166
>TC92156 similar to GP|15724296|gb|AAL06541.1 AT5g61660/k11j9_180
{Arabidopsis thaliana}, partial (67%)
Length = 650
Score = 27.3 bits (59), Expect = 4.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 10 SSGGGGFTSTQLNDSSPAPQKGRESQ 35
+SGGGG + N+ SP+ KG+ +Q
Sbjct: 410 NSGGGGHGGGRTNNRSPSESKGKTNQ 487
>TC87241 weakly similar to PIR|B71408|B71408 probable
acylaminoacyl-peptidase - Arabidopsis thaliana, partial
(47%)
Length = 1339
Score = 27.3 bits (59), Expect = 4.8
Identities = 24/102 (23%), Positives = 40/102 (38%), Gaps = 22/102 (21%)
Frame = -2
Query: 128 FQGVRQVAAFSVRPVVNFDEIPFHF--------IDCIHHHLRSKLKMEGTISTDPPASDS 179
F G A +++R F EIP + DC + S+ ++ + PP+
Sbjct: 666 FAGEGSSATYAIRVSSEFQEIPSNHPSA*TEPCFDCGTENSFSRQELLQISNLQPPSPSG 487
Query: 180 SL--------------KTPVRNTSNGSQAPSSTPAYAQYGVD 207
L +TPV +T NG+Q+ + P+ VD
Sbjct: 486 FLTIKSFEPDGDGTITETPVISTGNGAQSKPTEPSLRLLTVD 361
>TC86232 similar to GP|21593313|gb|AAM65262.1 protein disulfide isomerase
precursor-like {Arabidopsis thaliana}, partial (50%)
Length = 1315
Score = 26.6 bits (57), Expect = 8.2
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = -1
Query: 162 SKLKMEGTISTDPPASDSSLKTPVRNTSNGSQAPSSTPAYA 202
S LK ++S+ + ++ + +PV + SN + PSS P YA
Sbjct: 523 SDLKKGFSLSSRKSSPNALILSPVTSPSNTNFLPSSFPVYA 401
>BF650479 homologue to PIR|T06643|T066 hypothetical protein T20K18.200 -
Arabidopsis thaliana, partial (13%)
Length = 580
Score = 26.6 bits (57), Expect = 8.2
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = +2
Query: 46 NEASQSGDEKSSFVINGVELTNVTLVGMVFEKAE 79
NEA S E+S+ +++GVEL VGM F+ E
Sbjct: 326 NEADHSFLEESTHILDGVELQE-PYVGMEFDSEE 424
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,143,817
Number of Sequences: 36976
Number of extensions: 74549
Number of successful extensions: 573
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 568
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 572
length of query: 224
length of database: 9,014,727
effective HSP length: 93
effective length of query: 131
effective length of database: 5,575,959
effective search space: 730450629
effective search space used: 730450629
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0072a.1