BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0067a.8
(611 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 83 2e-32
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 112 3e-31
CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [i... 74 1e-13
BQ148771 58 9e-09
AW686588 56 4e-08
BG586862 54 2e-07
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 52 7e-07
BG456581 51 1e-06
BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At... 48 1e-05
BG584442 45 8e-05
TC80043 similar to GP|9759561|dbj|BAB11163.1 MtN21 nodulin prote... 44 2e-04
BG453645 41 0.001
BF520135 40 0.003
AW685555 37 0.022
BF650593 37 0.022
TC82948 37 0.022
TC82520 36 0.049
BE941032 35 0.11
TC77404 homologue to GP|493730|emb|CAA55318.1| lipoxygenase {Pis... 33 0.42
TC82430 weakly similar to SP|Q9LSU1|PSA5_ORYSA Proteasome subuni... 32 0.54
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 82.8 bits (203), Expect(3) = 2e-32
Identities = 54/174 (31%), Positives = 82/174 (47%), Gaps = 3/174 (1%)
Frame = +2
Query: 400 DMGE--WVGAGWRWKFSWRRNLSAREKGWVQKMCEEVGEVVLVQNRSDVWRWPPNSEGFY 457
DMG W G W+ W R L E+ V++ C + VL N +D WRW + Y
Sbjct: 383 DMGRLGWTVDGRAWE--WTRRLFVWEEECVRECCILLNNFVLQDNVNDKWRWLLDPVNGY 556
Query: 458 TVNNAYLFMQGDEGEPDAAL-KRLWAAPVPSNVKAFAWKLMLNRIPCRQQLQRRGVIDAQ 516
+V Y ++ D +L +W +PS V F W+L+ NR+P + L RGV+ A
Sbjct: 557 SVKVFYRYITSTGHISDRSLVDDVWHKHIPSKVSLFVWRLLRNRLPTKDNLVHRGVLLA- 733
Query: 517 SDVSCALCHEEVETSAHLMLSCSKVLGLWEACNTRLNSIYAPSLGPISEHFLHF 570
++ +C + E++ HL L C+ LW L I + S G + HF+ F
Sbjct: 734 TNAACVCGCVDSESTTHLFLHCNVFCSLWSLVRNWL-GIPSMSSGELRTHFIQF 892
Score = 51.2 bits (121), Expect(3) = 2e-32
Identities = 27/76 (35%), Positives = 41/76 (53%), Gaps = 3/76 (3%)
Frame = +3
Query: 334 SPWWRDVQAARVDMG-GI-NWFEAGLAKVVGNGDSTQFWLEDWSGKGKLDGRYHRLYNLS 391
S WW+ + R +G G+ NWFE + VVG+G FW + W+G L +Y RL +L+
Sbjct: 177 SMWWKTICKVREGVGEGVGNWFEENIRMVVGDGRDAFFWYDTWAGDVPLRLKYPRLLDLA 356
Query: 392 RQKYSRI-VDMGEWVG 406
K ++ + G W G
Sbjct: 357 MDKECKVGLTWGGWGG 404
Score = 44.3 bits (103), Expect(3) = 2e-32
Identities = 19/42 (45%), Positives = 28/42 (66%), Gaps = 4/42 (9%)
Frame = +1
Query: 297 FNTAVLGKCCWRYLCDTNSLWCQVIKAKYDQPG----RIIES 334
FN ++LGK CWR L D LW +V+KA+Y + G R+++S
Sbjct: 49 FNLSLLGKWCWRLLVDKEGLWHRVLKARYGEEGGGFVRVVDS 174
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 112 bits (281), Expect(2) = 3e-31
Identities = 65/218 (29%), Positives = 106/218 (47%), Gaps = 10/218 (4%)
Frame = -2
Query: 334 SPWWRDVQAARVDMGGINWFEAGLAKVVGNGDSTQFWLEDWSGKGKLDGRYHRLYNLSRQ 393
S WW+D+ + ++G + WF L + VG+G S+ FW + W L + R + R
Sbjct: 778 SRWWKDLMSLE-EVGRVRWFPRELIRKVGDGRSSFFWKDAWDSSVPLRESFPRAFFPYRL 602
Query: 394 KYSRIVDMGEWVGAGWRWKFSWRR-NLSAREKGWVQKMCEEVG--EVVLVQNRSDVWRWP 450
D+ + G RW+ WRR L EK +++ E +G E V+++ +D+W W
Sbjct: 601 LKMGCGDLWDMNAEGVRWRLYWRRLELFEWEK---ERLLELLGRLEGVVLRYWADIWVWK 431
Query: 451 PNSEGFYTVNNAYLFMQGDE-------GEPDAALKRLWAAPVPSNVKAFAWKLMLNRIPC 503
P+ EG ++VN+ Y +Q E + + LW + P+ V AF+W L L+RIP
Sbjct: 430 PDKEGVFSVNSCYFLLQNLRLLEDRLSYEEEVIFRELWKSKAPAKVLAFSWTLFLDRIPT 251
Query: 504 RQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCSKV 541
L +R ++ + C C + ET HL L C +
Sbjct: 250 MVNLGKRRLLRVEDSKRCVFCGCQDETVVHLFLHCDVI 137
Score = 40.8 bits (94), Expect(2) = 3e-31
Identities = 19/45 (42%), Positives = 28/45 (62%)
Frame = -3
Query: 281 CKPKDFGGLGLKDWLPFNTAVLGKCCWRYLCDTNSLWCQVIKAKY 325
C P+ GGLG++D N ++L K WR L D +SLW +V++ Y
Sbjct: 969 CLPRCKGGLGVRDIRLVNVSLLAKWWWRLLQDQSSLWKEVLEDIY 835
>CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [imported] -
Sulfolobus solfataricus, partial (3%)
Length = 789
Score = 74.3 bits (181), Expect = 1e-13
Identities = 39/129 (30%), Positives = 63/129 (48%), Gaps = 1/129 (0%)
Frame = -2
Query: 480 LWAAPVPSNVKAFAWKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCS 539
+W VP V FAW+L+ +R+P + L RGVI ++ + + C +E++ HL LSCS
Sbjct: 584 IWHRQVPLKVSVFAWRLLRDRLPTKSNLIYRGVIPTEAGLCVSGC-GALESAQHLFLSCS 408
Query: 540 KVLGLWEACNTRLNSIYAPSLGPISEHFLHFPHH-GRNATQRHGEYAVWLALTWSVWLLR 598
LW + + +S+HF+ F H G N + +WL W +W R
Sbjct: 407 YFASLWSLVRDWIGFV-GVDTNVLSDHFVQFVHSTGGNKASQSFLQLIWLLCAWVLWTER 231
Query: 599 NEILFGTAV 607
N + F ++
Sbjct: 230 NNMCFNDSI 204
>BQ148771
Length = 680
Score = 58.2 bits (139), Expect = 9e-09
Identities = 38/122 (31%), Positives = 55/122 (44%), Gaps = 15/122 (12%)
Frame = -3
Query: 246 IPVGVEKECQRIMWNFLWGGSPEERRIAWVSWERICKPKDFGGLGLKDWLPFNTAVLGKC 305
IP +E Q++ F+WG + RR V WE + KPK GLGL+ N A + K
Sbjct: 471 IPKACIEEIQKLQRKFVWGDTEVSRRYHAVGWETMSKPKTIYGLGLRRLDVMNKACIMKL 292
Query: 306 CWRYLCDTNSLWCQVIKAKYDQPGRIIE---------SPW------WRDVQAARVDMGGI 350
W +NSL +V++ KY + + E S W W +++ VD G
Sbjct: 291 GWSIYSGSNSLCTEVMRGKYQRSESLEEIFLEKPTDSSLWKALVKLWPEIERNLVDSNG- 115
Query: 351 NW 352
NW
Sbjct: 114 NW 109
>AW686588
Length = 567
Score = 56.2 bits (134), Expect = 4e-08
Identities = 34/120 (28%), Positives = 54/120 (44%), Gaps = 1/120 (0%)
Frame = +1
Query: 485 VPSNVKAFAWKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCSKVLGL 544
VP V AW+L+ +R+P + L RR + ++ C + ET+ HL L C+ +
Sbjct: 142 VPLKVSILAWRLIRDRLPTKANLVRRRCLAVEA-AGCVVGCGIAETANHLFLHCATFGAV 318
Query: 545 WEACNTRLNSIYAPSLGPISEHFLHF-PHHGRNATQRHGEYAVWLALTWSVWLLRNEILF 603
W+ + + +S+HF+ F G +R +WL W VW RN LF
Sbjct: 319 WQHIRAWI-GVSGADPHDLSDHFIQFITCTGHTRARRSFMQLIWLLCVWMVWNERNNRLF 495
>BG586862
Length = 804
Score = 53.9 bits (128), Expect = 2e-07
Identities = 33/128 (25%), Positives = 57/128 (43%), Gaps = 2/128 (1%)
Frame = -1
Query: 478 KRLWAAPVPSNVKAFAWKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLS 537
+++W K+F W+L+ N +P + +L +RG+ + + C C ++ET HL L+
Sbjct: 660 EKVWGIKTIPRHKSFLWRLLHNALPVKDELHKRGI---RCSLLCPRCESKIETVQHLFLN 490
Query: 538 CSKVLGLWEACNTRLNSIYAPSLGPISEHFLHFPHHGRNATQRHGEYAV--WLALTWSVW 595
C W +N S LHF N ++ E + AL +S+W
Sbjct: 489 CEVTQKEWFGSQLGINF--------HSSGVLHFHDWITNFILKNDEETIIALTALLYSIW 334
Query: 596 LLRNEILF 603
RN+ +F
Sbjct: 333 HARNQKVF 310
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 52.0 bits (123), Expect = 7e-07
Identities = 28/115 (24%), Positives = 48/115 (41%), Gaps = 10/115 (8%)
Frame = +3
Query: 449 WPPNSEGFYTVNNAYLFMQ----------GDEGEPDAALKRLWAAPVPSNVKAFAWKLML 498
W N G Y+V + Y ++ + K++W+ K W+++
Sbjct: 6 WMHNPTGIYSVKSGYNTLRTWQTQQINNTSTSSDETLIWKKIWSLHTIPRHKVLLWRILN 185
Query: 499 NRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCSKVLGLWEACNTRLN 553
+ +P R L++RG+ Q C CH + ET HL +SC +W N +N
Sbjct: 186 DSLPVRSSLRKRGI---QCYPLCPRCHSKTETITHLFMSCPLSKRVWFGSNLCIN 341
>BG456581
Length = 683
Score = 50.8 bits (120), Expect = 1e-06
Identities = 40/158 (25%), Positives = 64/158 (40%), Gaps = 5/158 (3%)
Frame = +2
Query: 454 EGFYTVNNAYLFMQGDEGEPDAALKRLWAAPVPSNVKAFAWKLMLNRIPCRQQLQRRGVI 513
+G T+ Y F+ A +W + +P + W+L +R+P L RG
Sbjct: 5 QGKLTIKLVYSFLTSHTSCAPWA-STIWNSCIPPSHSFICWRLAHDRLPTDDNLSSRGCA 181
Query: 514 DAQSDVSCALCHEEVETSAHLMLSCSKVLGLWEACNTRLN-----SIYAPSLGPISEHFL 568
C+ C E+VETS HL L C V+ LW ++L S + L + H
Sbjct: 182 LVSM---CSFCLEQVETSDHLFLRCKFVVTLWSWLCSQLRVGLDFSSFKALLSSLPRHC- 349
Query: 569 HFPHHGRNATQRHGEYAVWLALTWSVWLLRNEILFGTA 606
++ R A + + S+W RN + F +A
Sbjct: 350 -------SSQVRDLYVAAVVHMVHSIWWARNNVRFSSA 442
>BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (10%)
Length = 767
Score = 47.8 bits (112), Expect = 1e-05
Identities = 30/128 (23%), Positives = 50/128 (38%), Gaps = 11/128 (8%)
Frame = -3
Query: 445 DVWRWPPNSEGFYTVNNAYLFMQG-----------DEGEPDAALKRLWAAPVPSNVKAFA 493
D + W + G Y+V + Y D+ D +R+W V+ F
Sbjct: 426 DSYSWEYSKSGHYSVKSGYYVQTNIIAAANQRGTVDQPSLDDLYQRVWKYNTSPKVRHFL 247
Query: 494 WKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCSKVLGLWEACNTRLN 553
W+ + N +P ++ R + D SC+ C E ET H++ C +W +
Sbjct: 246 WRCISNSLPTAANMRSRHI---SKDGSCSRCGMESETVNHILFQCPYARLIWAT-----S 91
Query: 554 SIYAPSLG 561
I+AP G
Sbjct: 90 PIHAPPYG 67
>BG584442
Length = 775
Score = 45.1 bits (105), Expect = 8e-05
Identities = 29/93 (31%), Positives = 49/93 (52%), Gaps = 1/93 (1%)
Frame = +1
Query: 230 KCALVSPAVLFLSYFKIPVGVEKECQRIMWNFLWGGSPEERR-IAWVSWERICKPKDFGG 288
K AL S + +S F + E ++IM F W E R+ + W+S E++ K++GG
Sbjct: 439 KYALQSISSYVMSIFLLLNSQVDEIEKIMNTFSWVHVGENRKGMHWMS*EKLFVHKNYGG 618
Query: 289 LGLKDWLPFNTAVLGKCCWRYLCDTNSLWCQVI 321
+G D+ FN +LGK +L + +L+ + I
Sbjct: 619 MGFTDFTTFNIPMLGKQV*SFLLNRTTLFLEKI 717
>TC80043 similar to GP|9759561|dbj|BAB11163.1 MtN21 nodulin protein-like
{Arabidopsis thaliana}, partial (28%)
Length = 1421
Score = 43.5 bits (101), Expect = 2e-04
Identities = 20/51 (39%), Positives = 30/51 (58%)
Frame = +1
Query: 351 NWFEAGLAKVVGNGDSTQFWLEDWSGKGKLDGRYHRLYNLSRQKYSRIVDM 401
NW + KVV NG T FW E WS +G+ +Y RL+ + K +++VD+
Sbjct: 712 NWLSGSITKVVRNGRDTSFWSEKWS-RGR---QYSRLFKILLDKEAKVVDL 852
>BG453645
Length = 622
Score = 41.2 bits (95), Expect = 0.001
Identities = 25/89 (28%), Positives = 43/89 (48%), Gaps = 5/89 (5%)
Frame = +3
Query: 520 SCALCHEEVETSAHLMLSCSKVLGLWEACNT--RLNSIYAPSLGPISEHFLHFP--HHGR 575
+C LC+ +VET+ H+ L C +W +N + P+L F+H+ G
Sbjct: 30 TCVLCNLKVETTTHIFLHCDVASLVWFRVMKWFDVNFLIPPNL------FVHWECWSEGG 191
Query: 576 NATQ-RHGEYAVWLALTWSVWLLRNEILF 603
+A + G + VW W +W RN+++F
Sbjct: 192 SANRVTKGHWLVWHTTIWVLWAKRNDLIF 278
>BF520135
Length = 202
Score = 39.7 bits (91), Expect = 0.003
Identities = 21/64 (32%), Positives = 32/64 (49%)
Frame = +3
Query: 480 LWAAPVPSNVKAFAWKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCS 539
LW S V FAW+L R+P + + +RG++ + + C +E+ HL L C
Sbjct: 15 LWRKEDLSKVSIFAWRLFHGRLPTKANVFKRGIVHHDAHMCVTRC-RLIESDVHLFLHCD 191
Query: 540 KVLG 543
VLG
Sbjct: 192 -VLG 200
>AW685555
Length = 654
Score = 37.0 bits (84), Expect = 0.022
Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Frame = +3
Query: 516 QSDVSCALCHEEVETSAHLMLSCSKVLG---LWEACNTRLNSIYAPSLGPISEHFLHFPH 572
QS+ +E S H CS VLG L + C L++I++P+ PI H H+
Sbjct: 354 QSNDGKEFLEDEDSESQHQFSVCSSVLGRMELTDPCTDNLDTIFSPAFEPIEVHLQHYTE 533
Query: 573 HG 574
G
Sbjct: 534 KG 539
>BF650593
Length = 486
Score = 37.0 bits (84), Expect = 0.022
Identities = 17/54 (31%), Positives = 25/54 (45%)
Frame = +3
Query: 485 VPSNVKAFAWKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSC 538
VP V W+L N + R L +RGV+D Q+ + C E+ +H C
Sbjct: 306 VPLKVSCLVWRLFQNXLATRDNLSKRGVLD-QNSIXCVXDCGREESVSHFFFEC 464
>TC82948
Length = 705
Score = 37.0 bits (84), Expect = 0.022
Identities = 14/37 (37%), Positives = 22/37 (58%)
Frame = +3
Query: 271 RIAWVSWERICKPKDFGGLGLKDWLPFNTAVLGKCCW 307
++ VSWE++C+P G LG++ N A+ K CW
Sbjct: 255 KVVKVSWEKVCRPIKEGSLGIRSLSKLNEALNLKLCW 365
Score = 33.5 bits (75), Expect = 0.24
Identities = 13/60 (21%), Positives = 29/60 (47%)
Frame = +3
Query: 494 WKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCSKVLGLWEACNTRLN 553
W +M+++ ++ + D C+LC + E++ HL CS + +W+ + +N
Sbjct: 363 WDMMISKEQWYAFMRHNRALLGHIDFMCSLCFKSFESTHHLFFECSVSVQIWDWFSNLIN 542
>TC82520
Length = 833
Score = 35.8 bits (81), Expect = 0.049
Identities = 15/42 (35%), Positives = 24/42 (56%)
Frame = +3
Query: 480 LWAAPVPSNVKAFAWKLMLNRIPCRQQLQRRGVIDAQSDVSC 521
+W +PS V F W+L NR+P + L +R V+ Q+ +C
Sbjct: 186 VWQKNIPSKVSMFVWRLFHNRLPTKVNLMQRHVLQ-QTHTAC 308
>BE941032
Length = 435
Score = 34.7 bits (78), Expect = 0.11
Identities = 19/54 (35%), Positives = 27/54 (49%)
Frame = +2
Query: 492 FAWKLMLNRIPCRQQLQRRGVIDAQSDVSCALCHEEVETSAHLMLSCSKVLGLW 545
F W ++LNR P + L +RGVI A SC + + HL L C+ +W
Sbjct: 152 FLWCVLLNRFPTKDNLLKRGVISAIYQ-SCVGECGNLYDATHLFLHCNFFRQIW 310
>TC77404 homologue to GP|493730|emb|CAA55318.1| lipoxygenase {Pisum
sativum}, partial (85%)
Length = 2463
Score = 32.7 bits (73), Expect = 0.42
Identities = 15/40 (37%), Positives = 18/40 (44%), Gaps = 1/40 (2%)
Frame = -2
Query: 409 WRWKFSWRRNLSAREKGWV-QKMCEEVGEVVLVQNRSDVW 447
WRW F+W+ L +E W K C V N S VW
Sbjct: 182 WRWCFTWKIRLVGKENAWTFVKFCVVNPRVAYKSNGSMVW 63
>TC82430 weakly similar to SP|Q9LSU1|PSA5_ORYSA Proteasome subunit alpha
type 5 (EC 3.4.25.1) (20S proteasome alpha subunit E),
partial (77%)
Length = 561
Score = 32.3 bits (72), Expect = 0.54
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +3
Query: 284 KDFGGLGLKDWLPFNTAVLGKCCWRYLCDTNSLWCQVI 321
KD G+ +L F+ V GKC W + + +++WC +I
Sbjct: 213 KDQSGISHASYL*FSITVWGKCTW*RIHNESTVWCCII 326
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.336 0.146 0.539
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,540,496
Number of Sequences: 36976
Number of extensions: 494013
Number of successful extensions: 4479
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 3253
Number of HSP's successfully gapped in prelim test: 142
Number of HSP's that attempted gapping in prelim test: 1047
Number of HSP's gapped (non-prelim): 3611
length of query: 611
length of database: 9,014,727
effective HSP length: 102
effective length of query: 509
effective length of database: 5,243,175
effective search space: 2668776075
effective search space used: 2668776075
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0067a.8