BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0067a.6
(713 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF644046 49 5e-06
TC77387 similar to GP|15983396|gb|AAL11566.1 At1g51440/F5D21_19 ... 37 0.034
BQ137255 homologue to PIR|B34768|B347 ORF5 protein - Orf virus (... 35 0.100
BQ151362 weakly similar to GP|13561980|gb| flagelliform silk pro... 34 0.17
TC82080 similar to GP|9757854|dbj|BAB08488.1 contains similarity... 34 0.17
TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-inf... 34 0.22
TC78476 homologue to SP|O22437|CHLD_PEA Magnesium-chelatase subu... 33 0.29
BF641220 weakly similar to PIR|G86203|G86 probable N-arginine di... 33 0.29
TC93337 weakly similar to GP|21240660|gb|AAM44371.1 hypothetical... 33 0.29
BQ151366 similar to GP|20146300|dbj hypothetical protein~similar... 33 0.38
BI263340 weakly similar to PIR|T49223|T492 uclacyanin 3 [importe... 33 0.50
BI311490 weakly similar to GP|21751020|dbj unnamed protein produ... 32 0.65
AW683905 weakly similar to GP|9757854|dbj| contains similarity t... 32 0.65
TC80072 similar to GP|14423520|gb|AAK62442.1 Unknown protein {Ar... 32 0.85
TC85840 similar to SP|P35016|ENPL_CATRO Endoplasmin homolog prec... 32 1.1
TC87158 similar to GP|21426124|gb|AAM52321.1 Putative nascent po... 32 1.1
BF004047 weakly similar to GP|15027957|gb| putative arginine/ser... 31 1.9
TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown prot... 31 1.9
BF646260 similar to GP|7291176|gb|A CG15295 gene product {Drosop... 31 1.9
TC80693 homologue to PIR|T52063|T52063 ran GTPase-activating pro... 31 1.9
>BF644046
Length = 597
Score = 49.3 bits (116), Expect = 5e-06
Identities = 24/51 (47%), Positives = 29/51 (56%)
Frame = +3
Query: 141 VYGYFFLDLNIKLPFSPFICHVLTFLNVAPCQLQPNAWGFIRCFEILCEHL 191
+Y + F D+ K PF+ F C L LNVA QL PN F+ FEI CE L
Sbjct: 12 MYSFVFEDIGFKFPFTNFECDFLKALNVASSQLHPNCCAFMCGFEISCESL 164
>TC77387 similar to GP|15983396|gb|AAL11566.1 At1g51440/F5D21_19
{Arabidopsis thaliana}, partial (75%)
Length = 1793
Score = 36.6 bits (83), Expect = 0.034
Identities = 14/36 (38%), Positives = 25/36 (68%)
Frame = +3
Query: 662 SLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKENP 697
S + Q ++E E EEE+ ED E+ ++E++E+E+P
Sbjct: 297 SSSTMNQLHEEEEEEEEEDEDEEEEEEEEEEEEESP 404
>BQ137255 homologue to PIR|B34768|B347 ORF5 protein - Orf virus (strain NZ2),
partial (10%)
Length = 1161
Score = 35.0 bits (79), Expect = 0.100
Identities = 27/89 (30%), Positives = 40/89 (44%), Gaps = 3/89 (3%)
Frame = +2
Query: 373 AAEAEQKKKNEGTSGSDNV---RDPKRQKTSSATGGRPLHQSTQSTLDPKDRPAEKKKGH 429
A E ++K EGTS D+ R P+R + G P H++ Q+ R A+++ H
Sbjct: 83 ATEGAPRQKEEGTSARDDPPTPRAPRRPAKTPTE*GEP-HRTPQTH---HSRQAKRRPPH 250
Query: 430 DNVPPTQPDSSALINRPPTPFTQAGPSSA 458
PP P R P P GP++A
Sbjct: 251 HTPPPRNPRPPRPRGRRPRP--HGGPTAA 331
>BQ151362 weakly similar to GP|13561980|gb| flagelliform silk protein
{Argiope trifasciata}, partial (3%)
Length = 1136
Score = 34.3 bits (77), Expect = 0.17
Identities = 42/181 (23%), Positives = 61/181 (33%), Gaps = 22/181 (12%)
Frame = +1
Query: 342 HAATMGKSKVDANPIKMKEYLAQSAAMVKKRAAEAEQKKKNEGTSGSDNVRDPKRQKTSS 401
H T K + + P ++ A KK E E + E T +R
Sbjct: 136 HRGTRKKKRAGSRP--------KNTAQAKKTTGEREDPQPKEKTY--------QRSAAGV 267
Query: 402 ATGGRPLHQSTQSTLDPKDRPAEKKKGHDNVPPTQPDSSALINRPPTPFTQA-------- 453
ATG +T ++ K+ HD P P++ +N PP P T A
Sbjct: 268 ATGRESAGGTTNNS----------KRQHD---PPYPNTMPCVNHPPPPTTPAKAPPAPRD 408
Query: 454 -GPSSAIGG-------------ETPPLLSLSDPHFNGLDFMTRTFDNRLHKNVSGQGPPN 499
GP++ G PPLL +D + D R + + Q PPN
Sbjct: 409 SGPAAPTNGGGAKAPLRCRVACAFPPLLCCNDKNHLK*DVDRRRSTRKKTQK*KNQAPPN 588
Query: 500 I 500
I
Sbjct: 589 I 591
>TC82080 similar to GP|9757854|dbj|BAB08488.1 contains similarity to
apoptosis antagonizing transcription
factor~gene_id:MFB13.10, partial (37%)
Length = 908
Score = 34.3 bits (77), Expect = 0.17
Identities = 23/77 (29%), Positives = 36/77 (45%)
Frame = +2
Query: 618 SLQFAQGFLAAKEQIRMFEPGFDLSRIGYLKDIKDGQVIGDDDVSLDLLPQFNDESEPEE 677
SL G +AK+ +M + DL DDD+S + + ++ E EE
Sbjct: 2 SLSLKMGSRSAKKSKKMQKSDSDLDEY-------------DDDMSFNEVNDDSEVEEEEE 142
Query: 678 EDGEDRNEQNKDEDQEK 694
ED E +E+ +DE QE+
Sbjct: 143 EDDEGSDEEEEDERQEE 193
>TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-infected
erythrocyte surface antigen {Plasmodium falciparum},
partial (2%)
Length = 2007
Score = 33.9 bits (76), Expect = 0.22
Identities = 16/59 (27%), Positives = 32/59 (54%)
Frame = +1
Query: 648 KDIKDGQVIGDDDVSLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKENPQVGTSQGND 706
++IKDG+ I Q +E +EE + NE+NKDE++ ++ ++ ++G +
Sbjct: 172 EEIKDGEKI-----------QQENEENKDEEKSQQENEENKDEEKSQQENELKKNEGGE 315
Score = 28.5 bits (62), Expect = 9.4
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 9/50 (18%)
Frame = +1
Query: 670 NDESEPEEEDGEDR----NEQNKDEDQEK-ENPQV----GTSQGNDVNND 710
N+ES+ E E++ NE+NKDE++ K EN ++ Q N+ N D
Sbjct: 76 NEESKETGESSEEKSHLENEENKDEEKSKQENEEIKDGEKIQQENEENKD 225
>TC78476 homologue to SP|O22437|CHLD_PEA Magnesium-chelatase subunit chlD
chloroplast precursor (Mg- protoporphyrin IX chelatase),
partial (68%)
Length = 1596
Score = 33.5 bits (75), Expect = 0.29
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 668 QFNDESEPEEEDGEDRNEQNKDEDQEKENPQ 698
Q ++E + EEED E+ E+ D+D+EKE Q
Sbjct: 527 QESNEEQNEEEDKEEEEEEEDDKDEEKEEQQ 619
>BF641220 weakly similar to PIR|G86203|G86 probable N-arginine dibasic
convertase [imported] - Arabidopsis thaliana, partial
(5%)
Length = 634
Score = 33.5 bits (75), Expect = 0.29
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = +2
Query: 651 KDGQVIGDDDVSLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKENPQVGTSQGND 706
KDG + DD+ ++ E EE+D ED +E DED+E E+ G +
Sbjct: 344 KDGSIDEDDE---------EEDDEDEEDDEEDDDEGEDDEDEEXEDEDEXXVXGRE 484
>TC93337 weakly similar to GP|21240660|gb|AAM44371.1 hypothetical protein
{Dictyostelium discoideum}, partial (7%)
Length = 743
Score = 33.5 bits (75), Expect = 0.29
Identities = 20/61 (32%), Positives = 34/61 (54%)
Frame = +1
Query: 651 KDGQVIGDDDVSLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKENPQVGTSQGNDVNND 710
+DG+ G+DD DE++PE+ + DR+E+ DE +EKE +G++ N+
Sbjct: 58 EDGERGGEDDEM--------DENDPEKPEVVDRSEEFVDEGKEKE------EEGDEKENE 195
Query: 711 N 711
N
Sbjct: 196 N 198
>BQ151366 similar to GP|20146300|dbj hypothetical protein~similar to Oryza
sativa OSJNBa0058E19.20, partial (9%)
Length = 1137
Score = 33.1 bits (74), Expect = 0.38
Identities = 22/86 (25%), Positives = 35/86 (40%)
Frame = +3
Query: 364 QSAAMVKKRAAEAEQKKKNEGTSGSDNVRDPKRQKTSSATGGRPLHQSTQSTLDPKDRPA 423
Q ++R E++KK G+ P ++K +S +GG Q + +
Sbjct: 117 QDGGRRRQRGTTREKEKK-----GAPGPGPPAKKKAASRSGGGARGGGEQRNPRQRTKKE 281
Query: 424 EKKKGHDNVPPTQPDSSALINRPPTP 449
+K K N P T+ A N PP P
Sbjct: 282 KKNKRKTNQPKTEGARQASSNHPPPP 359
>BI263340 weakly similar to PIR|T49223|T492 uclacyanin 3 [imported] -
Arabidopsis thaliana, partial (14%)
Length = 681
Score = 32.7 bits (73), Expect = 0.50
Identities = 22/66 (33%), Positives = 29/66 (43%)
Frame = +3
Query: 407 PLHQSTQSTLDPKDRPAEKKKGHDNVPPTQPDSSALINRPPTPFTQAGPSSAIGGETPPL 466
P H T ST P + P+ G+ N PP+ P + PP+P T P + TPP
Sbjct: 312 PSHNQTPST--PSNPPSSG--GYYNSPPSTPTDPPVTLTPPSPSTPIDPGTPT-VPTPPF 476
Query: 467 LSLSDP 472
L P
Sbjct: 477 LPSPSP 494
>BI311490 weakly similar to GP|21751020|dbj unnamed protein product {Homo
sapiens}, partial (22%)
Length = 387
Score = 32.3 bits (72), Expect = 0.65
Identities = 20/48 (41%), Positives = 29/48 (59%)
Frame = -3
Query: 648 KDIKDGQVIGDDDVSLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKE 695
K KD + G+DD D +F +ES+PE ED E+ E++K+E E E
Sbjct: 343 KQEKDA-IDGEDDGEEDNNDEFFNESQPEFED-ENEKEESKEEIDESE 206
>AW683905 weakly similar to GP|9757854|dbj| contains similarity to apoptosis
antagonizing transcription factor~gene_id:MFB13.10,
partial (12%)
Length = 492
Score = 32.3 bits (72), Expect = 0.65
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = +3
Query: 658 DDDVSLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKEN 696
DDD+S D + ++ E +EEDGED E ++D ++EN
Sbjct: 150 DDDMSYDEVNDDSELEEEKEEDGEDEGE---EDDGQEEN 257
Score = 30.8 bits (68), Expect = 1.9
Identities = 24/81 (29%), Positives = 40/81 (48%), Gaps = 1/81 (1%)
Frame = +3
Query: 616 RASLQFAQ-GFLAAKEQIRMFEPGFDLSRIGYLKDIKDGQVIGDDDVSLDLLPQFNDESE 674
+ SL F + G +AK+ M + DL D+ +V +DD L+ + + E E
Sbjct: 54 KLSLGFTKMGSSSAKKSKNMQKSDSDLDEYDDDDDMSYDEV--NDDSELEEEKEEDGEDE 227
Query: 675 PEEEDGEDRNEQNKDEDQEKE 695
EE+DG++ N DE ++ E
Sbjct: 228 GEEDDGQEENGWRDDEMEQLE 290
>TC80072 similar to GP|14423520|gb|AAK62442.1 Unknown protein {Arabidopsis
thaliana}, partial (17%)
Length = 746
Score = 32.0 bits (71), Expect = 0.85
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = +1
Query: 649 DIKDGQVIGDDDVSLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKE 695
DI D+D D +P FND+ +E ED +E+ D+D++ E
Sbjct: 412 DINADSAESDED---DEMPIFNDKDIDNDETDEDGDEEEDDDDEDDE 543
>TC85840 similar to SP|P35016|ENPL_CATRO Endoplasmin homolog precursor
(GRP94 homolog). [Rosy periwinkle Madagascar
periwinkle], partial (92%)
Length = 2791
Score = 31.6 bits (70), Expect = 1.1
Identities = 13/42 (30%), Positives = 25/42 (58%)
Frame = +1
Query: 657 GDDDVSLDLLPQFNDESEPEEEDGEDRNEQNKDEDQEKENPQ 698
G +V +++ +DES EE+ E E+++DED +K+ +
Sbjct: 898 GSKEVDVEVPADEDDESSEEEDTTESPKEESEDEDADKDEDE 1023
>TC87158 similar to GP|21426124|gb|AAM52321.1 Putative nascent polypeptide
associated complex alpha chain {Oryza sativa (japonica
cultivar-group)}, partial (76%)
Length = 937
Score = 31.6 bits (70), Expect = 1.1
Identities = 14/36 (38%), Positives = 25/36 (68%)
Frame = +3
Query: 666 LPQFNDESEPEEEDGEDRNEQNKDEDQEKENPQVGT 701
LPQ D++ E+D +D ++++DE+ + E+PQ GT
Sbjct: 201 LPQEEDDAPIVEDDNDDDKDESEDEEDD-ESPQGGT 305
>BF004047 weakly similar to GP|15027957|gb| putative arginine/serine-rich
protein {Arabidopsis thaliana}, partial (11%)
Length = 835
Score = 30.8 bits (68), Expect = 1.9
Identities = 15/35 (42%), Positives = 24/35 (67%)
Frame = +1
Query: 676 EEEDGEDRNEQNKDEDQEKENPQVGTSQGNDVNND 710
E EDGE + +KDE+QEKE +V T + ++ +N+
Sbjct: 199 EREDGEVDGDDDKDEEQEKE-VEVETERSDEESNN 300
>TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown protein
{Arabidopsis thaliana}, partial (19%)
Length = 679
Score = 30.8 bits (68), Expect = 1.9
Identities = 10/45 (22%), Positives = 28/45 (62%)
Frame = +1
Query: 664 DLLPQFNDESEPEEEDGEDRNEQNKDEDQEKENPQVGTSQGNDVN 708
D + + + E + +E++ ED ++ + D+D ++++ G ++ D+N
Sbjct: 229 DEIEEMDFEDDEDEDEDEDEDDDDDDDDDDEDDDDDGFAEPTDLN 363
>BF646260 similar to GP|7291176|gb|A CG15295 gene product {Drosophila
melanogaster}, partial (4%)
Length = 670
Score = 30.8 bits (68), Expect = 1.9
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +2
Query: 648 KDIKDGQVI-GDDDVSLDLLPQFNDESEPEEEDGEDRNEQ 686
K KD +I DDD ++ + DE E + EDG+DR +Q
Sbjct: 422 KAFKDSLLIDSDDDDDDEVEEDYEDEDEDDNEDGDDRFKQ 541
>TC80693 homologue to PIR|T52063|T52063 ran GTPase-activating protein
[imported] - alfalfa, partial (30%)
Length = 735
Score = 30.8 bits (68), Expect = 1.9
Identities = 34/139 (24%), Positives = 61/139 (43%), Gaps = 16/139 (11%)
Frame = +2
Query: 570 DLAASDLLLQKTKSLKETINDKHTAAQAKCQKLEKKYE-------------RLNASILGR 616
D+A +D+ + S+ E I+ K + + E K E +L+ L
Sbjct: 107 DMAGNDITAKTAVSVAECISSKQFLTKLNLSENELKDEGAGLISKALEGLGQLSEVDLST 286
Query: 617 ASLQFAQGFLAAKEQIRMFEPGFDLSRIGYLKDIKDGQVIGDDDV---SLDLLPQFNDES 673
+ ++ L A+ ++ +PGF L + I D + D+ S D+L DE+
Sbjct: 287 NLITWSGAKLLAEAVVQ--KPGFKLLNVN-ANFISDEGIDELKDIFKNSPDILGPL-DEN 454
Query: 674 EPEEEDGEDRNEQNKDEDQ 692
+PE ED ++ E+N D D+
Sbjct: 455 DPEGEDIDEEAEENSDNDE 511
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.131 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,273,739
Number of Sequences: 36976
Number of extensions: 369650
Number of successful extensions: 2192
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 2018
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2147
length of query: 713
length of database: 9,014,727
effective HSP length: 103
effective length of query: 610
effective length of database: 5,206,199
effective search space: 3175781390
effective search space used: 3175781390
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0067a.6