BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0067a.13
(212 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86898 similar to GP|18376007|emb|CAB91741. probable ATP citrat... 37 0.004
TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like... 35 0.028
TC79385 similar to GP|9279590|dbj|BAB01048.1 gene_id:MPE11.2~unk... 35 0.028
TC88911 weakly similar to GP|13926280|gb|AAK49610.1 AT5g14920/F2... 35 0.028
BQ144407 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 34 0.036
TC85577 homologue to GP|6715512|gb|AAF26445.1| vacuolar H+-ATPas... 34 0.047
TC82224 homologue to PIR|H71404|H71404 hypothetical protein d131... 32 0.18
BQ158038 similar to GP|4775353|emb hyperpolarization-activated c... 31 0.31
TC91433 31 0.40
BG587084 homologue to GP|6002696|gb| histone acetyltransferase M... 31 0.40
TC83149 similar to GP|13561982|gb|AAK30594.1 flagelliform silk p... 31 0.40
BG454949 similar to GP|6440817|dbj| hypothetical protein {Chlamy... 30 0.68
TC79555 homologue to GP|10567859|gb|AAG18593.1 L8019.5 {Leishman... 30 0.68
BQ144498 similar to GP|6330472|dbj KIAA1205 protein {Homo sapien... 30 0.68
AL369139 weakly similar to GP|21554368|gb| unknown {Arabidopsis ... 30 0.89
TC92828 weakly similar to GP|4115538|dbj|BAA36412.1 UDP-glycose:... 30 0.89
BQ155388 similar to GP|20197493|gb|A expressed protein {Arabidop... 30 0.89
TC81043 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproli... 29 1.2
TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 ... 29 1.2
TC93249 weakly similar to GP|13877617|gb|AAK43886.1 protein kina... 29 1.2
>TC86898 similar to GP|18376007|emb|CAB91741. probable ATP citrate lyase
subunit 2 {Neurospora crassa}, complete
Length = 2389
Score = 37.4 bits (85), Expect = 0.004
Identities = 25/73 (34%), Positives = 37/73 (50%), Gaps = 1/73 (1%)
Frame = +3
Query: 5 PTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKP-PGPSTPTKSSQRPTVSQIVQS 63
PT + PP P++++G S+PV V P T P P P++PT S TVS +V
Sbjct: 1500 PTLMPRPVPPSSLLSSTPTAVSGLSSPVVVPPSSTPTPSPPPASPTSSPT--TVSTLVLP 1673
Query: 64 PAKSKSPLVPLSN 76
P+ + LS+
Sbjct: 1674 PSPRPTTTPVLSS 1712
>TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like protein
{Arabidopsis thaliana}, partial (16%)
Length = 814
Score = 34.7 bits (78), Expect = 0.028
Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = +3
Query: 13 PPLGSSRKLPSSITGSSAPVAVTPLKTIKPP--GPSTPTKSSQRPTVSQIVQSPAKSKSP 70
PP ++ P T S++P + I PP G +TP+ S+ P S SP SK+P
Sbjct: 114 PPPSTTPSAPPPSTPSNSPPSPPTTPAISPPSGGGTTPSPPSRTPPSSDDSPSPPSSKTP 293
Query: 71 LVP 73
P
Sbjct: 294 PPP 302
Score = 30.0 bits (66), Expect = 0.68
Identities = 30/100 (30%), Positives = 39/100 (39%), Gaps = 9/100 (9%)
Frame = +3
Query: 2 SEKPTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPG--------PSTPTKSSQ 53
S P + PP S+ P +T SAP TP PP PSTP+ S
Sbjct: 3 SSPPPATPSSPPP--STPANPPPVT-PSAPPPSTPATPSSPPPSTTPSAPPPSTPSNSPP 173
Query: 54 RPTVSQIVQSPA-KSKSPLVPLSNKYSILDYQNTISSKTP 92
P + + P+ +P P S D + SSKTP
Sbjct: 174SPPTTPAISPPSGGGTTPSPPSRTPPSSDDSPSPPSSKTP 293
Score = 26.2 bits (56), Expect = 9.8
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +3
Query: 5 PTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSS 52
PT+ P G + P S T S+ + +P + PP PS P+ SS
Sbjct: 180 PTTPAISPPSGGGTTPSPPSRTPPSSDDSPSPPSSKTPPPPSPPSSSS 323
>TC79385 similar to GP|9279590|dbj|BAB01048.1 gene_id:MPE11.2~unknown
protein {Arabidopsis thaliana}, partial (16%)
Length = 912
Score = 34.7 bits (78), Expect = 0.028
Identities = 16/35 (45%), Positives = 21/35 (59%)
Frame = -1
Query: 22 PSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPT 56
PS +T SSAP +++ + P PS P KSS PT
Sbjct: 654 PSPVTKSSAPPSLSDNNPVP*PNPSKPVKSSSNPT 550
>TC88911 weakly similar to GP|13926280|gb|AAK49610.1 AT5g14920/F2G14_40
{Arabidopsis thaliana}, partial (11%)
Length = 1320
Score = 34.7 bits (78), Expect = 0.028
Identities = 25/73 (34%), Positives = 36/73 (49%), Gaps = 2/73 (2%)
Frame = +2
Query: 3 EKPTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGP--STPTKSSQRPTVSQI 60
+ P SR Q+ P S +P + T +SAPV KPP P +TPT + +P V ++
Sbjct: 47 QSPKSRPQHLLPWKSP--VPKATTPTSAPV--------KPPVPKVATPTVAPAKPLVPKV 196
Query: 61 VQSPAKSKSPLVP 73
A P+VP
Sbjct: 197 TTPTAAPVKPIVP 235
>BQ144407 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (23%)
Length = 1217
Score = 34.3 bits (77), Expect = 0.036
Identities = 26/80 (32%), Positives = 32/80 (39%)
Frame = -3
Query: 8 RDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSPAKS 67
R PPL + +LP +P AV PL + GP P SS PT PA
Sbjct: 357 RHSAAPPLHAPYRLPHLFLHPHSPPAVIPLSLLPVQGPDLPPVSSPPPTPP---PPPAPP 187
Query: 68 KSPLVPLSNKYSILDYQNTI 87
+ P S SI +TI
Sbjct: 186 RPAAPPPSLSISINPAPHTI 127
Score = 27.3 bits (59), Expect = 4.4
Identities = 23/69 (33%), Positives = 27/69 (38%)
Frame = -1
Query: 5 PTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSP 64
PTS PP SS P + S AP++ L PP P P RP P
Sbjct: 314 PTSSSIRIPPPRSS---PYRCSRSKAPISPPFLLPPPPPRPPPPPPGRPRP--------P 168
Query: 65 AKSKSPLVP 73
S PL+P
Sbjct: 167 HPSPFPLIP 141
>TC85577 homologue to GP|6715512|gb|AAF26445.1| vacuolar H+-ATPase B subunit
{Nicotiana tabacum}, partial (14%)
Length = 841
Score = 33.9 bits (76), Expect = 0.047
Identities = 24/69 (34%), Positives = 30/69 (42%)
Frame = -1
Query: 2 SEKPTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIV 61
S PT PP S PS+++ PVA P+ P PS P SS P VS V
Sbjct: 475 SSSPTISPVATPPKSSQAPSPSAVS----PVASPPVPVKNAPSPSPPA-SSDSPAVSPAV 311
Query: 62 QSPAKSKSP 70
+ S +P
Sbjct: 310 TPSSISTTP 284
>TC82224 homologue to PIR|H71404|H71404 hypothetical protein d13195c -
Arabidopsis thaliana, partial (1%)
Length = 562
Score = 32.0 bits (71), Expect = 0.18
Identities = 25/69 (36%), Positives = 34/69 (49%), Gaps = 3/69 (4%)
Frame = +3
Query: 4 KPTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPST---PTKSSQRPTVSQI 60
KPT+ + PL +SRKLPS T SS + LK P ST P +Q+P I
Sbjct: 66 KPTTTTRSITPLTTSRKLPSLNTSSS-----SSLKENNNPRRSTSRGPIHPTQKPLTRSI 230
Query: 61 VQSPAKSKS 69
++ +S S
Sbjct: 231 PRTDRRSSS 257
>BQ158038 similar to GP|4775353|emb hyperpolarization-activated cyclic
nucleotide gated cation channel hHCN4 {Homo sapiens},
partial (1%)
Length = 1060
Score = 31.2 bits (69), Expect = 0.31
Identities = 19/54 (35%), Positives = 23/54 (42%)
Frame = +2
Query: 3 EKPTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPT 56
+K TS Q G P P TG+ APVA K + PP + Q PT
Sbjct: 227 KKSTSVRQPGGPKPPRFPAPGDPTGTPAPVAAVQAKRVPPPRTGRRYREMQ*PT 388
>TC91433
Length = 738
Score = 30.8 bits (68), Expect = 0.40
Identities = 26/89 (29%), Positives = 38/89 (42%), Gaps = 8/89 (8%)
Frame = +2
Query: 14 PLGSSRKLPSSITGSS-------APVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSPAK 66
P ++ K P++ + +S A + L T P PST Q+P V+ S K
Sbjct: 350 PASNTNKQPAATSPTSNKPITTVTAAAPSKLPTTNPSAPSTLPLDKQKPIVAATPASSTK 529
Query: 67 S-KSPLVPLSNKYSILDYQNTISSKTPGK 94
+ P+SNK S TIS+ P K
Sbjct: 530 QPPAATSPISNKPS-----TTISAAAPSK 601
>BG587084 homologue to GP|6002696|gb| histone acetyltransferase MORF beta
{Homo sapiens}, partial (1%)
Length = 685
Score = 30.8 bits (68), Expect = 0.40
Identities = 19/58 (32%), Positives = 32/58 (54%)
Frame = +1
Query: 79 SILDYQNTISSKTPGKTEQYEYQEKPECLPSVMIEKAWLNCNPRDIAKQVFPPNFHYM 136
++ +++NTI SK PG + Q P + VM W+ C+ R+I +QVF + H +
Sbjct: 106 TLREFENTIRSKGPGNAPFFGGQLHP-LVRFVMNFLTWI-CDYREILEQVFEDHGHVL 273
>TC83149 similar to GP|13561982|gb|AAK30594.1 flagelliform silk protein
{Argiope trifasciata}, partial (3%)
Length = 1177
Score = 30.8 bits (68), Expect = 0.40
Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 6/54 (11%)
Frame = +3
Query: 26 TGSSAP--VAVTPLKTIKPPGPSTPT----KSSQRPTVSQIVQSPAKSKSPLVP 73
T S++P + TPL++ P PS+PT ++S R T S +SK+PL P
Sbjct: 12 TPSTSPSYASRTPLRSTPPTRPSSPTPSAPRTSSRSTPSTSPSRRPRSKTPLPP 173
Score = 30.4 bits (67), Expect = 0.52
Identities = 22/64 (34%), Positives = 26/64 (40%), Gaps = 2/64 (3%)
Frame = +3
Query: 13 PPLGSSRKLPSSITGSSAPVAVT--PLKTIKPPGPSTPTKSSQRPTVSQIVQSPAKSKSP 70
PP S ++ S S+P A P +T PP PSTP S P S SP
Sbjct: 204 PPQKSPPRMRRSKPSPSSPAATPLWPQRTTPPPSPSTPRPSP*TPA--------TPSTSP 359
Query: 71 LVPL 74
PL
Sbjct: 360 TAPL 371
Score = 27.7 bits (60), Expect = 3.4
Identities = 18/67 (26%), Positives = 26/67 (37%)
Frame = +3
Query: 13 PPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSPAKSKSPLV 72
P + LP+ ++ P A+TP +PP PSTP P A + P
Sbjct: 345 PSTSPTAPLPTPPPRTTLPPALTP----RPPSPSTPPTPKPGPVSVSPASPSATQRVPWR 512
Query: 73 PLSNKYS 79
P + S
Sbjct: 513 PTARASS 533
Score = 26.6 bits (57), Expect = 7.5
Identities = 21/61 (34%), Positives = 26/61 (42%)
Frame = +3
Query: 14 PLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSPAKSKSPLVP 73
P SSR PS+ + S P + TPL PP P P K Q+ P+ S P
Sbjct: 99 PRTSSRSTPST-SPSRRPRSKTPLPPPPPPLPQHP-KPPQKSPPRMRRSKPSPSSPAATP 272
Query: 74 L 74
L
Sbjct: 273 L 275
>BG454949 similar to GP|6440817|dbj| hypothetical protein {Chlamydomonas sp.
HS-5}, partial (69%)
Length = 676
Score = 30.0 bits (66), Expect = 0.68
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = -3
Query: 55 PTVSQIVQSPAKSKSPLVPLSNKYSILDYQNTI 87
PT S++ SPA+++SPLVP + D ++ +
Sbjct: 470 PTASRVATSPAQARSPLVPREYSPPLADQESGV 372
>TC79555 homologue to GP|10567859|gb|AAG18593.1 L8019.5 {Leishmania major},
partial (1%)
Length = 1410
Score = 30.0 bits (66), Expect = 0.68
Identities = 23/73 (31%), Positives = 32/73 (43%), Gaps = 10/73 (13%)
Frame = +2
Query: 2 SEKPTSRDQYGPPLGSSRKLPSSITGSSAPVAVT-PLKTIKPPGPSTPTKS--------- 51
S KPT P S+R P+ + S+P T P T KP TP++S
Sbjct: 449 SPKPTLVPP*SPQPSSTRSSPAPPSRPSSPATTTRPPPTSKPSPTQTPSRSPSSARTPST 628
Query: 52 SQRPTVSQIVQSP 64
S PT + ++ SP
Sbjct: 629 SSTPTRAPLLSSP 667
Score = 27.3 bits (59), Expect = 4.4
Identities = 18/75 (24%), Positives = 31/75 (41%)
Frame = +2
Query: 5 PTSRDQYGPPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSP 64
PTS+ P + + PSS S T + P P P ++RP + +SP
Sbjct: 560 PTSKPS---PTQTPSRSPSSARTPSTSSTPTRAPLLSSPPPPPPFFPARRPGTRSLPKSP 730
Query: 65 AKSKSPLVPLSNKYS 79
+ + +P + + S
Sbjct: 731 PRPRPARLPSARRAS 775
>BQ144498 similar to GP|6330472|dbj KIAA1205 protein {Homo sapiens}, partial
(3%)
Length = 1284
Score = 30.0 bits (66), Expect = 0.68
Identities = 33/139 (23%), Positives = 52/139 (36%), Gaps = 22/139 (15%)
Frame = -1
Query: 30 APVAVTPLKTI-------KPPGPSTPTKSSQRPTVSQIVQSPAK-------------SKS 69
AP+ ++PL ++ +P S S + P V+ I ++P + S S
Sbjct: 1251 APLLLSPLLSLILIASPFQPSSHSRSAYSRRAPCVALIARAPIRPRLALSIDTRAPFSSS 1072
Query: 70 PLVPLSNKYSILDYQNTISSK-TPGKTEQYEYQEKPE-CLPSVMIEKAWLNCNPRDIAKQ 127
PL P Y T + P + P CLPS + + D+A+
Sbjct: 1071 PLFPSRLAYCCRRPYLTFRPRHLPTSPHSTDSASSPRSCLPSAPLHLPPIFST--DLARA 898
Query: 128 VFPPNFHYMSGHPSKGRLF 146
PP +HY+ P LF
Sbjct: 897 YTPPRYHYIYRRPHPPDLF 841
>AL369139 weakly similar to GP|21554368|gb| unknown {Arabidopsis thaliana},
partial (11%)
Length = 388
Score = 29.6 bits (65), Expect = 0.89
Identities = 13/53 (24%), Positives = 25/53 (46%)
Frame = -2
Query: 18 SRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSPAKSKSP 70
S LP S++ ++P +P + P P+TPT + + +P ++ P
Sbjct: 387 SSSLPPSLSSFASPSFSSPSSLLHSPNPNTPTPNQTARIYRSLTPNPNRNPIP 229
>TC92828 weakly similar to GP|4115538|dbj|BAA36412.1 UDP-glycose:flavonoid
glycosyltransferase {Vigna mungo}, partial (20%)
Length = 649
Score = 29.6 bits (65), Expect = 0.89
Identities = 26/76 (34%), Positives = 35/76 (45%), Gaps = 7/76 (9%)
Frame = +1
Query: 21 LPSSITGSSAPVAVTPLKTIKPPGPS-----TPTKSSQRPTVS--QIVQSPAKSKSPLVP 73
LPSS T S TP +PP S TP+ S+Q S ++ P+ S S ++P
Sbjct: 199 LPSSSTVSLPSPLTTPPPKAEPPPHSISSNQTPSTSNQNSVKSPKPLLSKPSSSISSVLP 378
Query: 74 LSNKYSILDYQNTISS 89
+ YQ TISS
Sbjct: 379 PWKQLPPC*YQFTISS 426
>BQ155388 similar to GP|20197493|gb|A expressed protein {Arabidopsis
thaliana}, partial (49%)
Length = 498
Score = 29.6 bits (65), Expect = 0.89
Identities = 21/71 (29%), Positives = 32/71 (44%)
Frame = +2
Query: 18 SRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSPAKSKSPLVPLSNK 77
SR S+ S P A +PL ++PP +TP T ++ PA S L L
Sbjct: 35 SRSFISTARSSLRPAAPSPLPRLRPPPLATPR------TQPRLFSMPATSSRNLGQLGCV 196
Query: 78 YSILDYQNTIS 88
S+L +T++
Sbjct: 197QSLLPLHSTMA 229
>TC81043 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproline-rich
glycoprotein DZ-HRGP {Volvox carteri f. nagariensis},
partial (19%)
Length = 555
Score = 29.3 bits (64), Expect = 1.2
Identities = 19/69 (27%), Positives = 25/69 (35%), Gaps = 4/69 (5%)
Frame = +1
Query: 13 PPLGSSRKLPSSITGSSAPVAVTPLKTIKPPGPSTPTKSSQRPTVSQIVQSPAK----SK 68
PP S P + +P A P PP P P P+ +P K
Sbjct: 220 PPSPGSPPKPPAPPSPGSPPAPAPAPPPIPPSPPAPPSPGSPPSPPPASPAPPKPTPPKG 399
Query: 69 SPLVPLSNK 77
SPL+P+ K
Sbjct: 400 SPLLPMPEK 426
>TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 - alfalfa,
partial (97%)
Length = 1049
Score = 29.3 bits (64), Expect = 1.2
Identities = 19/68 (27%), Positives = 30/68 (43%), Gaps = 2/68 (2%)
Frame = +3
Query: 5 PTSRDQYGPPLGSSRKLPSSITGSSAPVAVTP--LKTIKPPGPSTPTKSSQRPTVSQIVQ 62
PT+ PP+ SS P + S P+ +P ++ PP S+P Q P + +
Sbjct: 300 PTTPQASPPPVQSS---PPPVQSSPPPLQSSPPPAQSTPPPVQSSPPPVQQSPPPTPLTP 470
Query: 63 SPAKSKSP 70
P +S P
Sbjct: 471 PPVQSTPP 494
Score = 28.1 bits (61), Expect = 2.6
Identities = 21/67 (31%), Positives = 26/67 (38%), Gaps = 6/67 (8%)
Frame = +3
Query: 13 PPLGSS----RKLPSSITGSSAPVAVTPLKT--IKPPGPSTPTKSSQRPTVSQIVQSPAK 66
PPL SS + P + S PV +P T PP STP +S P P
Sbjct: 366 PPLQSSPPPAQSTPPPVQSSPPPVQQSPPPTPLTPPPVQSTPPPASPPPASPPPFSPPPA 545
Query: 67 SKSPLVP 73
+ P P
Sbjct: 546 TPPPATP 566
>TC93249 weakly similar to GP|13877617|gb|AAK43886.1 protein kinase-like
protein {Arabidopsis thaliana}, partial (5%)
Length = 669
Score = 29.3 bits (64), Expect = 1.2
Identities = 20/54 (37%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +3
Query: 19 RKLPSSITGSSAPVAVTPLKTIKPPGPS-TPTKSSQRPTVSQIVQSPAKSKSPL 71
R L SS+ SS+ + TP K KPP P +PT S S + SP+ L
Sbjct: 60 RCLTSSVRTSSSATSSTPPKFSKPPPPPPSPTTSPTSSPFSAPLPSPSSPNGVL 221
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.312 0.130 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,980,734
Number of Sequences: 36976
Number of extensions: 105684
Number of successful extensions: 724
Number of sequences better than 10.0: 91
Number of HSP's better than 10.0 without gapping: 686
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 718
length of query: 212
length of database: 9,014,727
effective HSP length: 92
effective length of query: 120
effective length of database: 5,612,935
effective search space: 673552200
effective search space used: 673552200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0067a.13