BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0065.6
(516 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87745 similar to PIR|F84452|F84452 probable nonsense-mediated ... 596 0.0
TC79383 weakly similar to GP|12643043|gb|AAK00432.1 hypothetical... 178 4e-45
BE318443 76 2e-16
BG447717 46 4e-05
BF635945 38 0.011
TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 33 0.26
TC83125 similar to PIR|T05841|T05841 spliceosome-associated prot... 31 1.0
TC88396 weakly similar to GP|18616497|emb|CAD22847. unnamed prot... 30 2.2
CB893779 similar to GP|10177269|db gb|AAC32911.1~gene_id:MJB21.3... 30 2.9
BF637940 29 3.8
TC89729 similar to GP|2318131|gb|AAB66486.1| histone deacetylase... 29 3.8
AW981356 similar to GP|21537293|gb GDSL-motif lipase/hydrolase-l... 29 5.0
BQ151358 similar to GP|11993889|gb| virion-associated nuclear-sh... 29 5.0
BI267042 28 6.5
TC78683 similar to GP|14194147|gb|AAK56268.1 AT4g17486/AT4g17486... 28 6.5
BE248845 weakly similar to PIR|T48246|T48 ribonuclease II-like p... 28 6.5
TC82828 similar to GP|15293289|gb|AAK93755.1 unknown protein {Ar... 28 8.5
>TC87745 similar to PIR|F84452|F84452 probable nonsense-mediated mRNA decay
protein [imported] - Arabidopsis thaliana, partial (77%)
Length = 1902
Score = 596 bits (1537), Expect(3) = 0.0
Identities = 284/331 (85%), Positives = 313/331 (93%)
Frame = +3
Query: 1 MFGSSEEAGMFTVHRTVGSVLCCKCGIPMQPNAANMCVKCLRSEVDITEGLLKRLVLVHC 60
+F ++EAGMF VH+T+GSVLCCKCGI MQPNAANMCVKCLRSEVDITEGLLKRLVLVHC
Sbjct: 96 IFKMAQEAGMFKVHQTIGSVLCCKCGIRMQPNAANMCVKCLRSEVDITEGLLKRLVLVHC 275
Query: 61 PECESYLQPPRTWIKLQLESKELLTFCLKKLQKNMSSSKVRMVHAEFIWTEPHSKRIKVN 120
PECESYLQPPRTW+KLQLESKELLTFCLKKLQKNM+ +K ++V+AEFIWTEPHSKR+KV
Sbjct: 276 PECESYLQPPRTWVKLQLESKELLTFCLKKLQKNMNINKAKLVNAEFIWTEPHSKRVKVK 455
Query: 121 VKVQKEVLNGAILEQSYLVEYVQQEHLCESCSKVAANPDQWVAAVQLRQHVSHRRTFFYL 180
V VQKEV +GAILEQSY VEYVQQ+H+CESCS+VAANPDQW+AAVQLRQHVSHRRTF+YL
Sbjct: 456 VSVQKEVYHGAILEQSYPVEYVQQDHMCESCSRVAANPDQWIAAVQLRQHVSHRRTFYYL 635
Query: 181 EQLILKHGAAASAIRIKQMDEGIDFYFSNRSHGVKFVEFIGKVAPIKSRSDKQLVSHDTK 240
EQLIL+HGAAA AIRIKQMD GIDFYFSNRSHG+KFVEF+GKVAP+KSRSDKQLVSHD+K
Sbjct: 636 EQLILRHGAAARAIRIKQMDHGIDFYFSNRSHGIKFVEFVGKVAPVKSRSDKQLVSHDSK 815
Query: 241 SNEYNYKHTFSVEISPICREDLICLPPKVALSLGNLGPIVICTKVTNSIALLDPFTLRYC 300
SN YNYK+TFSVEISPICREDLICLPPK A LGN+GPIVICTKVTNSIALLDP TL++
Sbjct: 816 SNNYNYKYTFSVEISPICREDLICLPPKAAAILGNIGPIVICTKVTNSIALLDPLTLKFG 995
Query: 301 FLDADQYWRSSFKSLLTSRQLVEYIVLDVEV 331
FLDA+QYWR+SFKSLLTSRQLVEYIVLDVEV
Sbjct: 996 FLDAEQYWRTSFKSLLTSRQLVEYIVLDVEV 1088
Score = 233 bits (593), Expect(3) = 0.0
Identities = 121/179 (67%), Positives = 143/179 (79%), Gaps = 5/179 (2%)
Frame = +2
Query: 343 YVLADAQVARVSDFGKNDTIFSIKTHLGHLLNPGDYALGYDLYGANNNDIELDKYKGLIL 402
Y LADAQVARVSDFGKNDT+FSI+THLGHLL PGD ALGYDLY AN+ND+ELD+YKG I
Sbjct: 1124 YALADAQVARVSDFGKNDTMFSIRTHLGHLLKPGDNALGYDLYAANSNDMELDRYKGNI- 1300
Query: 403 PDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENP 462
P+ +L+KKSY EKR K RS+KLK + ME DDK+ + + DK +EYE FLK+LEENP
Sbjct: 1301 PEVVLIKKSYPEKR-----KTRSFKLKRIPMEADDKI-KVNEDKRSAEYELFLKELEENP 1462
Query: 463 DLRFNISLYRNKEYQPSEIASVTDGEELPSVPLEELLA-----DLELGEDEDEEDDMME 516
+LRFNISLY+NK+YQPSE+ASVTDGEE PSVP +ELL DL GE +D ED+M E
Sbjct: 1463 ELRFNISLYQNKDYQPSEVASVTDGEEAPSVPWDELLTGLEDLDLSEGEGDDGEDNMAE 1639
Score = 22.7 bits (47), Expect(3) = 0.0
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = +1
Query: 332 VSSEVTVGGTK 342
VSSEV VGGTK
Sbjct: 1090 VSSEVNVGGTK 1122
>TC79383 weakly similar to GP|12643043|gb|AAK00432.1 hypothetical protein
{Oryza sativa}, partial (8%)
Length = 1082
Score = 178 bits (452), Expect = 4e-45
Identities = 137/382 (35%), Positives = 182/382 (46%), Gaps = 35/382 (9%)
Frame = +2
Query: 153 KVAANPDQWVAAVQLRQ-HVSHRRTFFYLEQL-ILKH-GAAASAIRIK-QMDEGIDFYFS 208
KV+ + + A V +RQ HVS RT F+LEQ+ + H G + RI+ ++EG+ FYF+
Sbjct: 5 KVSKMEEGFCAVVNVRQQHVSELRTLFHLEQVSVWNHLGIVGNFRRIEYNINEGLSFYFN 184
Query: 209 NRSHGVKFVEFIGKVAPIKSRSDKQLVSHDTKSNEYNYKHTFSVEISPICREDLICLPPK 268
+ S + V I + P + + +K + FSVEI PIC DLI LP
Sbjct: 185 SLSDAAEVVNLIVDILPAQVQWNKI------------DPYLFSVEIVPICCGDLIILPSN 328
Query: 269 VALSLG-NLGPIVICTKVTNSIALLDPFTLRYCFLDADQYWRSSFKSLLTSRQLVEYIVL 327
+A SLG N+GPIVICT+V + LLDPFTL +CFL A QYW + F + QLVEY VL
Sbjct: 329 IAASLGPNIGPIVICTRVAKTFTLLDPFTLTHCFLKAGQYWDAPFTPSFSRPQLVEYAVL 508
Query: 328 DVEVVSSE-----------------------------VTVGGTKYVLADAQVARVSDFGK 358
+E + E T KY LA+A VARV D G
Sbjct: 509 GIEKLDEEEEEEVEENKQKKKLVFKDEHDEDGDAFATYTAAAKKYRLANAIVARVKDIGN 688
Query: 359 NDTIFSIKTHLGHLLNPGDYALGYDLYGANNNDIELDKYKGLILPDAILVKKSYEEKRQK 418
N+ F I+THLG +L PGD+ALGYDL D IL+ K
Sbjct: 689 NNHTFQIRTHLGRILKPGDHALGYDLPEG----------------DPILITKI------- 799
Query: 419 KRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQFLKDLEENPDLRFN-ISLYRNKEYQ 477
S E + +V DK S Y+ FLKDL+++P L F+ ++YRN Y
Sbjct: 800 -----------SFAKEENGRVVPVQ-DKWESGYQLFLKDLQQDPKLLFDQRAIYRNPTYC 943
Query: 478 PSEIASVTDGEELPSVPLEELL 499
P PLE+LL
Sbjct: 944 HPSGPLFRSPFYRPFFPLEDLL 1009
>BE318443
Length = 502
Score = 75.9 bits (185), Expect(2) = 2e-16
Identities = 53/134 (39%), Positives = 76/134 (56%), Gaps = 5/134 (3%)
Frame = +2
Query: 161 WVAAVQLRQ-HVSHRRTFFYLEQL-ILKHGAAASAIR--IKQMDEGIDFYFSNRSHGVKF 216
W + V++RQ HVS RT F+LEQ+ ++ + + I +D+G F F+ S F
Sbjct: 20 WRSVVKVRQQHVSQLRTVFHLEQISVMTNLGILDDVEKIIYDVDQGFSFCFNIISCAWYF 199
Query: 217 VEFIGKVAPIKSRSDKQLVSHDTKSNEYNYKHTFSVEISPICREDLICLPPKVALSLG-N 275
IG + P+KS + ++ T+ ++ FSVEI PIC DLI LPP V SLG N
Sbjct: 200 ATLIGDIVPVKS--NWAMIIPRTE------RYLFSVEIVPICCGDLIFLPPNVVASLGPN 355
Query: 276 LGPIVICTKVTNSI 289
+GPIVICT+ +I
Sbjct: 356 IGPIVICTRGCQNI 397
Score = 28.1 bits (61), Expect(2) = 2e-16
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +1
Query: 299 YCFLDADQYWRSSF 312
+CFLDA QYW++ F
Sbjct: 427 HCFLDAHQYWKAPF 468
>BG447717
Length = 407
Score = 45.8 bits (107), Expect = 4e-05
Identities = 30/84 (35%), Positives = 47/84 (55%), Gaps = 4/84 (4%)
Frame = +3
Query: 153 KVAANPDQWVAAVQLRQ-HVSHRRTFFYLEQL-ILKH-GAAASAIRIK-QMDEGIDFYFS 208
KV+ + W A V +RQ HVS RT F+LEQ+ + H G + RI+ ++EG+ FYF+
Sbjct: 147 KVSKMEEGWRAVVNVRQQHVSELRTLFHLEQVSVWNHLGIVGNFRRIEYNINEGLSFYFN 326
Query: 209 NRSHGVKFVEFIGKVAPIKSRSDK 232
+ S + V I + P + +K
Sbjct: 327 SLSDAAEVVNLIXDILPAQVXXNK 398
>BF635945
Length = 296
Score = 37.7 bits (86), Expect = 0.011
Identities = 26/75 (34%), Positives = 37/75 (48%), Gaps = 6/75 (8%)
Frame = +1
Query: 161 WVAAVQLRQ-HVSHRRTFFYLEQLILKHGAA--ASAIRIKQM---DEGIDFYFSNRSHGV 214
WV + +RQ HV RT F+LEQ+ + + +RIK DEG+ F F++ S
Sbjct: 61 WVGVLNVRQQHVCQLRTLFHLEQISVWTNLTMLSHIMRIKNKIDDDEGLSFCFNSLSDAT 240
Query: 215 KFVEFIGKVAPIKSR 229
V I + P K R
Sbjct: 241 DVVNLIVDILPSKVR 285
>TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (13%)
Length = 663
Score = 33.1 bits (74), Expect = 0.26
Identities = 31/120 (25%), Positives = 54/120 (44%), Gaps = 3/120 (2%)
Frame = +3
Query: 395 DKYKGLILPDAILVKKSYEEKRQKK--RGKARSWKLKSLNMEVDDKVARADPDKMHSEY- 451
DK G + D + ++K E K +K K + K K +VD+ + D +K E
Sbjct: 99 DKSAGDVKEDKVEIEKDLEIKSVEKDDEKKEKKDKEKKDKTDVDEGKDKKDKEKKKKEKK 278
Query: 452 EQFLKDLEENPDLRFNISLYRNKEYQPSEIASVTDGEELPSVPLEELLADLELGEDEDEE 511
E+ +K EE+ D + + + ++ + + DGEE S +E D +DE E+
Sbjct: 279 EENVKGEEEDGDEKKDKEKKKKEKKEKGKEDKDKDGEEKKSKKDKEKKKDKNEDDDEGED 458
>TC83125 similar to PIR|T05841|T05841 spliceosome-associated protein homolog
F17L22.120 - Arabidopsis thaliana, partial (24%)
Length = 722
Score = 31.2 bits (69), Expect = 1.0
Identities = 27/118 (22%), Positives = 48/118 (39%), Gaps = 8/118 (6%)
Frame = +2
Query: 404 DAILVKKSYEEKRQKKRGKAR------SWKLKSLNMEVDDKVARADPDKMHSEYEQFLKD 457
+ ++ KKS E R+++RGKA+ ++ E D+ DP ++ + E ++
Sbjct: 71 NGVVAKKSKENDRRRRRGKAKKNNKASEQPASNIGEESDNAKENTDPKQVFEQVE--IEY 244
Query: 458 LEENPDLRFNISLYRNKEYQPSEIASVTDGEEL--PSVPLEELLADLELGEDEDEEDD 513
+ E DL + K ++ V EE V E + D D ED+
Sbjct: 245 VPEKVDLYEGMDEEFRKIFEKFSFTEVAASEETDKKDVAEETAATKKKANSDSDYEDE 418
>TC88396 weakly similar to GP|18616497|emb|CAD22847. unnamed protein product
{Triticum aestivum}, partial (32%)
Length = 1399
Score = 30.0 bits (66), Expect = 2.2
Identities = 22/77 (28%), Positives = 37/77 (47%)
Frame = +2
Query: 394 LDKYKGLILPDAILVKKSYEEKRQKKRGKARSWKLKSLNMEVDDKVARADPDKMHSEYEQ 453
LDKY +PD +++K EE+++ K+ + ME+ D+V +A
Sbjct: 875 LDKYLDDDMPDVFVLEKGTEEEQRAKKARF---------MELKDEVQKA----------- 994
Query: 454 FLKDLEENPDLRFNISL 470
F KD+ EN F+ +L
Sbjct: 995 FHKDMAENNQSGFSSAL 1045
>CB893779 similar to GP|10177269|db gb|AAC32911.1~gene_id:MJB21.3~similar to
unknown protein {Arabidopsis thaliana}, partial (19%)
Length = 932
Score = 29.6 bits (65), Expect = 2.9
Identities = 20/69 (28%), Positives = 36/69 (51%)
Frame = +2
Query: 57 LVHCPECESYLQPPRTWIKLQLESKELLTFCLKKLQKNMSSSKVRMVHAEFIWTEPHSKR 116
++HC C ++ ++++ KE+ KKL++++ S VRMV E+ +
Sbjct: 524 MLHCLNCVQRIRVLIYLLEIKHLRKEIEVLR*KKLRRHIVGSTVRMVGLEY------QIK 685
Query: 117 IKVNVKVQK 125
IKV+ KV K
Sbjct: 686 IKVSCKVAK 712
>BF637940
Length = 598
Score = 29.3 bits (64), Expect = 3.8
Identities = 18/60 (30%), Positives = 28/60 (46%)
Frame = -1
Query: 70 PRTWIKLQLESKELLTFCLKKLQKNMSSSKVRMVHAEFIWTEPHSKRIKVNVKVQKEVLN 129
P + LQ E+ L+ + L L+ N SS + PH+ R+K+NV Q + N
Sbjct: 544 PVVY*SLQYENWNLVGYYLTTLKPNQSSKAYKKTQKNM----PHNMRLKINV*KQVDCFN 377
>TC89729 similar to GP|2318131|gb|AAB66486.1| histone deacetylase
{Arabidopsis thaliana}, partial (12%)
Length = 800
Score = 29.3 bits (64), Expect = 3.8
Identities = 16/30 (53%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = +2
Query: 488 EELPSVPLEELLADLELGE-DEDEEDDMME 516
+ PSVP +E + EL E DEDEEDD E
Sbjct: 113 QHAPSVPFQERPPNTELQERDEDEEDDRDE 202
>AW981356 similar to GP|21537293|gb GDSL-motif lipase/hydrolase-like
protein {Arabidopsis thaliana}, partial (35%)
Length = 379
Score = 28.9 bits (63), Expect = 5.0
Identities = 11/30 (36%), Positives = 16/30 (52%)
Frame = +1
Query: 2 FGSSEEAGMFTVHRTVGSVLCCKCGIPMQP 31
+ + G FT RT+G ++ K GIP P
Sbjct: 19 YSGGQATGRFTNGRTIGDIISSKLGIPSPP 108
>BQ151358 similar to GP|11993889|gb| virion-associated nuclear-shuttling
protein {Mus musculus}, partial (3%)
Length = 1195
Score = 28.9 bits (63), Expect = 5.0
Identities = 12/48 (25%), Positives = 27/48 (56%)
Frame = +1
Query: 215 KFVEFIGKVAPIKSRSDKQLVSHDTKSNEYNYKHTFSVEISPICREDL 262
+ E+ + API R + +++T S+ Y++T ++ SP+ R+ +
Sbjct: 739 RITEYRKRYAPIHLRRPRPRRTYNTPSHNPQYRYTHNILCSPLVRDSI 882
>BI267042
Length = 403
Score = 28.5 bits (62), Expect = 6.5
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = -1
Query: 306 QYWRSSFKSLLTSRQLVEYIVLDVEVVSSEVTV 338
QY++ + K L ++ Y +L VEVV+ ++TV
Sbjct: 169 QYFKINIKKLAVKMEIYMYWILTVEVVTRKITV 71
>TC78683 similar to GP|14194147|gb|AAK56268.1 AT4g17486/AT4g17486
{Arabidopsis thaliana}, partial (74%)
Length = 1134
Score = 28.5 bits (62), Expect = 6.5
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = -2
Query: 406 ILVKKSYEEKRQKKRGKARSWKLKS 430
IL+ +KR+ +RGK R+WKL+S
Sbjct: 143 ILLPSKR*KKREGRRGKGRNWKLES 69
>BE248845 weakly similar to PIR|T48246|T48 ribonuclease II-like protein -
Arabidopsis thaliana, partial (26%)
Length = 653
Score = 28.5 bits (62), Expect = 6.5
Identities = 16/49 (32%), Positives = 24/49 (48%)
Frame = +1
Query: 84 LTFCLKKLQKNMSSSKVRMVHAEFIWTEPHSKRIKVNVKVQKEVLNGAI 132
+T ++K Q NM S + EF W E H K V V+ E++ G +
Sbjct: 73 ITHFVQKAQYNMDPSLL-----EFAWFELHEKNKSVTVEEMAEIIFGRV 204
>TC82828 similar to GP|15293289|gb|AAK93755.1 unknown protein {Arabidopsis
thaliana}, partial (62%)
Length = 719
Score = 28.1 bits (61), Expect = 8.5
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -1
Query: 63 CESYLQPPRTWIKLQLES 80
C SYL PP WI++ ++S
Sbjct: 467 CTSYLSPPTIWIRIAIDS 414
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,488,715
Number of Sequences: 36976
Number of extensions: 240863
Number of successful extensions: 1312
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1301
length of query: 516
length of database: 9,014,727
effective HSP length: 100
effective length of query: 416
effective length of database: 5,317,127
effective search space: 2211924832
effective search space used: 2211924832
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0065.6