BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0061.9
(277 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78223 weakly similar to GP|9758310|dbj|BAB08784.1 porin-like p... 343 e-123
TC87582 similar to PIR|S36454|S36454 porin por1 - garden pea, co... 246 9e-66
TC77259 similar to PIR|T12558|T12558 porin - common ice plant, p... 245 1e-65
TC79617 weakly similar to GP|9757871|dbj|BAB08458.1 porin-like p... 168 6e-62
TC85992 similar to GP|5031279|gb|AAD38145.1| porin {Prunus armen... 222 1e-58
TC84272 weakly similar to PIR|S46959|S46959 porin I 36K - potat... 94 6e-20
TC81600 weakly similar to SP|P07144|PORI_NEUCR Outer mitochondri... 64 5e-11
AW684657 similar to GP|9757871|dbj| porin-like protein {Arabidop... 51 4e-07
AW584306 weakly similar to PIR|T09116|T091 voltage-dependent ani... 40 8e-04
BI270961 similar to GP|9757871|dbj| porin-like protein {Arabidop... 28 3.9
TC82499 SP|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation f... 28 3.9
TC90456 weakly similar to PIR|G86466|G86466 hypothetical protein... 27 6.6
>TC78223 weakly similar to GP|9758310|dbj|BAB08784.1 porin-like protein
{Arabidopsis thaliana}, partial (82%)
Length = 1503
Score = 343 bits (881), Expect(2) = e-123
Identities = 171/211 (81%), Positives = 190/211 (90%), Gaps = 2/211 (0%)
Frame = +2
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
MANGPA + EIGKRARDLLYKDYNFD KF+LSIPSSTGLGL ATGLK+D+ F+GD+NTLY
Sbjct: 110 MANGPAPFSEIGKRARDLLYKDYNFDHKFSLSIPSSTGLGLTATGLKRDKFFVGDLNTLY 289
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIF--RCKKVALSFNIPDHKSGKLDVQYLHPHAAID 118
KSGN+TVDVKV+T SNVSTKVTLND+ KKVALSFNIPDHKSGKLDVQYLHPHAAID
Sbjct: 290 KSGNVTVDVKVNTDSNVSTKVTLNDVSLSHSKKVALSFNIPDHKSGKLDVQYLHPHAAID 469
Query: 119 SSIGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDK 178
SSIGLNP+P+LELS AIGSKD+ MGAEVGF+TTSASF+ YNAGI FN+PDFSA LML DK
Sbjct: 470 SSIGLNPAPKLELSAAIGSKDISMGAEVGFDTTSASFSTYNAGIAFNKPDFSATLMLADK 649
Query: 179 GKSLKASYIHHVDRPDGFTVAAEINHRFSSF 209
G+SLKASYIH+VDRPDG TVAAE+ H+F F
Sbjct: 650 GQSLKASYIHYVDRPDGLTVAAELAHKFVLF 742
Score = 115 bits (287), Expect(2) = e-123
Identities = 57/71 (80%), Positives = 60/71 (84%)
Frame = +3
Query: 207 SSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFLCQRAWRPKSLITLSAEYDSTKIFGSS 266
SS ENRFT G+SQ IDP TVLKTRFSDDGKAAF CQRAWRP SLITLSAEYDS KI GS
Sbjct: 735 SSSENRFTFGTSQSIDPKTVLKTRFSDDGKAAFQCQRAWRPNSLITLSAEYDSKKIIGSP 914
Query: 267 TKLGLALALQP 277
K GLAL+L+P
Sbjct: 915 AKFGLALSLKP 947
>TC87582 similar to PIR|S36454|S36454 porin por1 - garden pea, complete
Length = 1307
Score = 246 bits (627), Expect = 9e-66
Identities = 124/276 (44%), Positives = 174/276 (62%)
Frame = +1
Query: 2 ANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLYK 61
A GP LY +IGK+ARDLL+KDYN DQKF +S S TG+ + ++G KK ++F+GD+NT K
Sbjct: 64 AKGPGLYTDIGKKARDLLFKDYNSDQKFTVSTYSPTGVAITSSGTKKGELFVGDVNTQLK 243
Query: 62 SGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSSI 121
+ N T D+KVDT SN+ T +T+ + K +S +PD SGK+++QYLH +A I +I
Sbjct: 244 NKNYTADIKVDTESNLFTTITVTEPAPGVKAIISCKVPDPASGKVELQYLHDYAGISGNI 423
Query: 122 GLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGKS 181
GL +P + LS G+ L G ++ ++T TK N G++F + D AL L +KG
Sbjct: 424 GLKANPVVNLSGVFGTNALAFGGDISYDTKLRELTKSNVGVSFIKDDLLGALTLNEKGDV 603
Query: 182 LKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFLC 241
L ASY H ++ V ++ HRFS+ EN FT+G +DP T LK R ++ KA+ L
Sbjct: 604 LNASYYHVINPVTNTAVGVDMGHRFSTRENNFTLGVQHALDPLTTLKGRITNSAKASALI 783
Query: 242 QRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q WRPKSLIT+S E D TK S K+GL+L L+P
Sbjct: 784 QHEWRPKSLITISTELD-TKAIEKSAKVGLSLVLKP 888
>TC77259 similar to PIR|T12558|T12558 porin - common ice plant, partial
(98%)
Length = 1113
Score = 245 bits (626), Expect = 1e-65
Identities = 130/279 (46%), Positives = 178/279 (63%), Gaps = 2/279 (0%)
Frame = +1
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M+ GP LY +IGK+ARDLLYKDYN DQKF L+ S TG+ L A+ KK ++F+ DINT
Sbjct: 61 MSKGPGLYSDIGKKARDLLYKDYNTDQKFTLTTYSPTGIALTASSTKKGELFLADINTQL 240
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDH--KSGKLDVQYLHPHAAID 118
K N+T D+KVDT SN+ T +T+ + K LSF IP+ KSGK +VQYLH +A I
Sbjct: 241 KHKNVTTDIKVDTDSNLFTTITVTEPAPGLKTILSFRIPEPELKSGKAEVQYLHDYAGIS 420
Query: 119 SSIGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDK 178
+SIGL +P S IG+ + +G ++ ++T + FT+ NAG+ F + D A+L L +K
Sbjct: 421 ASIGLTANPVANFSGVIGNNVVALGGDLSYDTKTGVFTRLNAGLNFTKDDLIASLTLNEK 600
Query: 179 GKSLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAA 238
+L ASY H V+ V AE++HRFS+ EN T+G+ ID T K RF++ G A+
Sbjct: 601 ANALNASYYHVVNPVTKTAVGAEVSHRFSTKENTLTLGTQHAIDSLTTAKLRFNNFGLAS 780
Query: 239 FLCQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
L Q WRPKS T+S E D TK S ++G+ALAL+P
Sbjct: 781 GLIQHEWRPKSFFTISGEVD-TKAIEKSARIGIALALKP 894
>TC79617 weakly similar to GP|9757871|dbj|BAB08458.1 porin-like protein
{Arabidopsis thaliana}, partial (97%)
Length = 1208
Score = 168 bits (426), Expect(2) = 6e-62
Identities = 83/179 (46%), Positives = 116/179 (64%)
Frame = +2
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M+ GP LY +IGK+A+DLL KDYN DQK +S S+TG+ L +T LKK + GD+ Y
Sbjct: 68 MSKGPGLYSDIGKKAKDLLTKDYNSDQKLTVSSYSTTGVALTSTALKKGGLSTGDVAGQY 247
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K N +DVK++T S ++T +T D+F K S +PD+ SGKL+VQY H HA + S
Sbjct: 248 KYKNTLIDVKIETASIINTTLTFTDLFPSTKTIASIKLPDYSSGKLEVQYFHDHATLTSV 427
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKG 179
L SP ++S +GS L GAE G++T S SFTKYN GI+F + D SA++++ +G
Sbjct: 428 ATLKQSPIFDVSATVGSPTLAFGAEAGYDTKSGSFTKYNTGISFTKLDSSASVIIWRQG 604
Score = 86.7 bits (213), Expect(2) = 6e-62
Identities = 44/101 (43%), Positives = 61/101 (59%)
Frame = +3
Query: 177 DKGKSLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGK 236
DKG S+K SY+HH+D+ +I RFS+ +N T+G S +DP T +K RF++ GK
Sbjct: 597 DKGDSIKVSYLHHLDQIKKSAAVVDITRRFSTNQNTITVGGSFAVDPLTQIKARFNNQGK 776
Query: 237 AAFLCQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
L Q PKS++T+S E D TK K GLA+AL+P
Sbjct: 777 VGGLLQHELFPKSVLTISGEVD-TKALEKKPKFGLAIALKP 896
>TC85992 similar to GP|5031279|gb|AAD38145.1| porin {Prunus armeniaca},
partial (88%)
Length = 1293
Score = 222 bits (566), Expect = 1e-58
Identities = 114/277 (41%), Positives = 172/277 (61%)
Frame = +1
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M GP LY +IGKRARDLL+KDY D KF ++ + G+ + + G++K + F+ D++T
Sbjct: 67 MVKGPGLYSDIGKRARDLLFKDYQNDHKFTITTQTLAGVEITSAGVRKGEAFLADVSTKV 246
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
K+ N+T D+KVDT SN+ T +T+++ K SF P+ KSGK+++QYLH +A I +S
Sbjct: 247 KNKNVTTDIKVDTNSNLRTTITVDETVPGLKTIFSFIYPEQKSGKVELQYLHDYAGISTS 426
Query: 121 IGLNPSPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALMLVDKGK 180
IGL +P + +S +G+ + +G++V F T+S S K N G+ D A+L + D+G
Sbjct: 427 IGLTATPTVNISAVLGNNLVSVGSDVSFETSSGSLNKCNFGLNVTHADLIASLTVNDRGD 606
Query: 181 SLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDDGKAAFL 240
SL ASY H V V AE++H FSS EN TIG+ +DP T+LK + ++ GKA+ L
Sbjct: 607 SLNASYYHVVSPLTNTAVGAELSHSFSSNENVLTIGTQHALDPVTLLKAKVNNYGKASAL 786
Query: 241 CQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQP 277
Q W ++ +L E D+ I + K+GLA AL+P
Sbjct: 787 IQHDWSRQARFSLVGEVDTAAI-QKTAKVGLACALKP 894
>TC84272 weakly similar to PIR|S46959|S46959 porin I 36K - potato, partial
(7%)
Length = 759
Score = 94.0 bits (232), Expect = 6e-20
Identities = 73/264 (27%), Positives = 114/264 (42%), Gaps = 6/264 (2%)
Frame = +2
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDINTLY 60
M+ P +Y +G A+D+LYKDY + I+ Y
Sbjct: 62 MSKSPGVYSNLGDNAKDVLYKDY---------------------------VNQSPIHFHY 160
Query: 61 KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQYLHPHAAIDSS 120
+ +D + S KV +I + +PD SGK+++QYL+ I
Sbjct: 161 QF--------MDWNAGFSCKV--QEILPGFRTVFKCTVPD--SGKVELQYLNRFTGITGC 304
Query: 121 IGLNPS------PQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALM 174
IGL + P ++LS +G+ L +GA V + + S TK AG N A+L
Sbjct: 305 IGLLGNEEGAYDPVVKLSGLLGTSILSLGANVALHIPTRSITKLTAGFGLNTAFLEASLT 484
Query: 175 LVDKGKSLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSDD 234
L D +LKA+ H V+ +A ++ H S E TIG+ P T+LK RF
Sbjct: 485 LHDSFDTLKATIYHEVNPLTQTAIATQVKHSLSLKETGVTIGAQHAFFPETLLKARFDSS 664
Query: 235 GKAAFLCQRAWRPKSLITLSAEYD 258
GK L Q+ + + +T+++E D
Sbjct: 665 GKVGALIQQGFWQRFFVTMASEID 736
>TC81600 weakly similar to SP|P07144|PORI_NEUCR Outer mitochondrial membrane
protein porin. {Neurospora crassa}, partial (10%)
Length = 963
Score = 64.3 bits (155), Expect = 5e-11
Identities = 60/283 (21%), Positives = 116/283 (40%), Gaps = 11/283 (3%)
Frame = +3
Query: 5 PALYPEIGKRARDLLYKDYNFDQ-KFNLSIPSSTGLGLIATGLK--KDQIFIGDINTLY- 60
PA Y +IGK A DL KD+ K + G+ TGLK K G++ + Y
Sbjct: 33 PATYSDIGKSANDLFSKDFPVGSFKLESKTTAPNGVSFTLTGLKDTKSGNINGELKSKYY 212
Query: 61 ---KSGNLTVDVKVDTYSNVSTKVTLNDIFRCKKVALSFNIPDHKSGKLDVQ--YLHPHA 115
+ G T N ++ N + + K+ L NI SG +V+ +
Sbjct: 213 YPKRGGTFTGSWTTSNILNGQVELE-NSLAKGLKLDLVANIQP-ASGNRNVRGGFTFKQP 386
Query: 116 AIDSSIGLNP--SPQLELSTAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAAL 173
A+ + I L+ P + +G + +G+E ++ T+Y + P+++ AL
Sbjct: 387 ALHTKIYLDALKGPTFQGDIVVGHEGFLIGSEGCYDVLEGKLTRYGVTAGYTHPEYNVAL 566
Query: 174 MLVDKGKSLKASYIHHVDRPDGFTVAAEINHRFSSFENRFTIGSSQLIDPNTVLKTRFSD 233
+ + + Y H V A + + ++ G+ +D + +K +
Sbjct: 567 RVTNSLSAYTLLYYHRVSPEIEAGAKAHWDSQTNNPSVNLEFGAKYFLDRDAFIKGKVDS 746
Query: 234 DGKAAFLCQRAWRPKSLITLSAEYDSTKIFGSSTKLGLALALQ 276
GK +A RP I+L D++++ ++ + G+++ L+
Sbjct: 747 TGKVGIGYTQALRPGVKISLGGSIDTSRLNENAHRFGISINLE 875
>AW684657 similar to GP|9757871|dbj| porin-like protein {Arabidopsis
thaliana}, partial (14%)
Length = 548
Score = 51.2 bits (121), Expect = 4e-07
Identities = 23/39 (58%), Positives = 30/39 (75%)
Frame = +2
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGL 39
M+ GP LY +IGK+A+DLL KDYN DQK +S S+TG+
Sbjct: 86 MSKGPGLYSDIGKKAKDLLTKDYNSDQKLTVSSYSTTGV 202
>AW584306 weakly similar to PIR|T09116|T091 voltage-dependent anion channel
protein - spinach, partial (19%)
Length = 185
Score = 40.4 bits (93), Expect = 8e-04
Identities = 19/56 (33%), Positives = 32/56 (56%)
Frame = +3
Query: 1 MANGPALYPEIGKRARDLLYKDYNFDQKFNLSIPSSTGLGLIATGLKKDQIFIGDI 56
+ GP L+ I +RAR LL++DY F L+ S +G+ + ++GL + F D+
Sbjct: 18 VVRGPGLFSYIRERARYLLFRDYQSAHVFTLTSHSLSGVDISSSGLGRGDAFFADV 185
>BI270961 similar to GP|9757871|dbj| porin-like protein {Arabidopsis
thaliana}, partial (6%)
Length = 494
Score = 28.1 bits (61), Expect = 3.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +3
Query: 205 RFSSFENRFTIGSSQLIDPNT 225
RFS+ EN T+G S ++DP T
Sbjct: 3 RFSTNENTLTVGCSYVVDPQT 65
>TC82499 SP|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation factor 3
subunit 8 (eIF3 p110) (eIF3c). [Barrel medic], partial
(91%)
Length = 2816
Score = 28.1 bits (61), Expect = 3.9
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -1
Query: 126 SPQLELS-TAIGSKDLCMGAEVGFNTTSASFTKYNAGITFNRPDFSAALM 174
SP L +S T K L +VGF TS+ T AGI + F ++L+
Sbjct: 1775 SPYLYISDTNTSIKVLLFSGKVGFRATSSRVTTNGAGILVSSKPFGSSLI 1626
>TC90456 weakly similar to PIR|G86466|G86466 hypothetical protein AAD39608.1
[imported] - Arabidopsis thaliana, partial (2%)
Length = 1332
Score = 27.3 bits (59), Expect = 6.6
Identities = 15/49 (30%), Positives = 21/49 (42%)
Frame = -1
Query: 93 ALSFNIPDHKSGKLDVQYLHPHAAIDSSIGLNPSPQLELSTAIGSKDLC 141
A + N+ H ++ HPH + SPQ+ LST K LC
Sbjct: 1230 AKNLNLESHAKNPSTLKLFHPHQFL--------SPQMNLSTRPEHKQLC 1108
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,590,304
Number of Sequences: 36976
Number of extensions: 95125
Number of successful extensions: 411
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 407
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 407
length of query: 277
length of database: 9,014,727
effective HSP length: 95
effective length of query: 182
effective length of database: 5,502,007
effective search space: 1001365274
effective search space used: 1001365274
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0061.9