BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0060.17
(128 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89278 similar to GP|9757719|dbj|BAB08244.1 CRN (crooked neck) ... 201 6e-53
TC91329 similar to GP|7682783|gb|AAF67364.1| Hypothetical protei... 36 0.005
TC89508 similar to GP|18071414|gb|AAL58273.1 putative pre-mRNA s... 34 0.018
TC86389 similar to GP|12322855|gb|AAG51417.1 unknown protein; 78... 28 1.3
TC89964 weakly similar to PIR|B96639|B96639 protein T1F9.16 [imp... 27 1.7
BF633350 similar to GP|17473640|gb| unknown protein {Arabidopsis... 27 2.8
CA859660 similar to GP|10241917|dbj 34 kDa protein {Hop latent v... 26 3.7
TC87238 similar to PIR|H96781|H96781 unknown protein F22H5.20 [i... 26 4.8
AW686502 weakly similar to PIR|B84742|B8 probable receptor-like ... 25 8.2
TC82414 similar to PIR|T49915|T49915 pre-mRNA splicing factor AT... 25 8.2
BE997694 similar to GP|13646986|db DNA-binding protein DF1 {Pisu... 25 8.2
TC89377 similar to GP|10177940|dbj|BAB11299. gene_id:MQD19.5~unk... 25 8.2
BF632466 25 8.2
>TC89278 similar to GP|9757719|dbj|BAB08244.1 CRN (crooked neck) protein
{Arabidopsis thaliana}, partial (64%)
Length = 1612
Score = 201 bits (511), Expect = 6e-53
Identities = 98/116 (84%), Positives = 106/116 (90%)
Frame = +1
Query: 11 QKFSFANMWLLAAQFEIRQLNLKGAHVILGSAIGKAPKDKILKKYIEIELQLGNIDRCRK 70
QKFSFA +WLLAAQFEIRQLNL GA ILG+AIGKAPKDKI KKYIEIELQLGNIDRCRK
Sbjct: 601 QKFSFAKIWLLAAQFEIRQLNLTGARQILGNAIGKAPKDKIFKKYIEIELQLGNIDRCRK 780
Query: 71 LYEKYLEWALENCYAWSKYA*LERSLSETDRARSIFELAISQPASDMPELLWKVNI 126
LYEKYLEW+ ENCYAWSKYA LERSL+ET+RAR+IFELAI+QPA DMPEL+WK I
Sbjct: 781 LYEKYLEWSPENCYAWSKYAELERSLAETERARAIFELAIAQPALDMPELMWKAYI 948
Score = 36.2 bits (82), Expect = 0.004
Identities = 34/119 (28%), Positives = 50/119 (41%), Gaps = 17/119 (14%)
Frame = +1
Query: 19 WLLAAQFEIRQLNLKGAHVILGSAIGKAPKDK---ILKKYIEIELQLGNIDRCRKLYEKY 75
W A+ E + A I AI + D + K YI+ E +R R LYE+
Sbjct: 826 WSKYAELERSLAETERARAIFELAIAQPALDMPELMWKAYIDFETAECEFERARALYERL 1005
Query: 76 LEWALENCYAWSKYA*LERS-------LSETD-------RARSIFELAISQPASDMPEL 120
L+ ++ W YA E + LSE + RAR +FE A++ S P+L
Sbjct: 1006LD-RTKHLKVWQSYAEFEATAIDESLELSEQEQKEQCLQRARKVFEDALNHFRSSAPDL 1179
Score = 29.3 bits (64), Expect = 0.44
Identities = 17/68 (25%), Positives = 32/68 (47%), Gaps = 10/68 (14%)
Frame = +1
Query: 55 YIEIELQLGNIDRCRKLYEKYL---------EWALENCYAWSKYA*LER-SLSETDRARS 104
YI +E +GN +R R++YE+ + + Y W YA E + +R R
Sbjct: 388 YIRLEESVGNKERTREVYERAIANVPPAEEKRYWQRYIYLWINYALYEELDAGDMERTRD 567
Query: 105 IFELAISQ 112
+++ ++Q
Sbjct: 568 VYKECLNQ 591
>TC91329 similar to GP|7682783|gb|AAF67364.1| Hypothetical protein T32B20.g
{Arabidopsis thaliana}, partial (38%)
Length = 1068
Score = 35.8 bits (81), Expect = 0.005
Identities = 25/80 (31%), Positives = 42/80 (52%), Gaps = 5/80 (6%)
Frame = +3
Query: 42 AIGKAPKDKILKKYIEIELQLGNIDRCRKLYEKYLEWALENCYAWS---KYA*LERSLSE 98
A+ K + + YI ++ + + R++YE+ +E L + + KYA LERSL E
Sbjct: 714 AVPNNEKLSMYEIYIARAAEIFGVPKTREIYEQAIESGLPDKDVKTMCLKYAELERSLGE 893
Query: 99 TDRARSIFELA--ISQPASD 116
+RAR ++ A + P SD
Sbjct: 894 IERARGVYVFASKFADPRSD 953
Score = 29.3 bits (64), Expect = 0.44
Identities = 20/85 (23%), Positives = 40/85 (46%), Gaps = 21/85 (24%)
Frame = +3
Query: 14 SFANMWLLAAQFEIRQLNLKGAHVILG--------------SAIGKAPKDKILKK----- 54
+ A++W A+ E++ N KGA ++ +A G P L K
Sbjct: 132 NLASVWCEWAEIELKHENFKGALDLMRRATAEPSVEVKRKVAADGNQPVQMKLHKSLRLW 311
Query: 55 --YIEIELQLGNIDRCRKLYEKYLE 77
++++E LG+++ R++YE+ L+
Sbjct: 312 TFFVDLEESLGSLESTREVYERILD 386
>TC89508 similar to GP|18071414|gb|AAL58273.1 putative pre-mRNA splicing
factor {Oryza sativa (japonica cultivar-group)}, partial
(35%)
Length = 1470
Score = 33.9 bits (76), Expect = 0.018
Identities = 22/81 (27%), Positives = 37/81 (45%)
Frame = +1
Query: 18 MWLLAAQFEIRQLNLKGAHVILGSAIGKAPKDKILKKYIEIELQLGNIDRCRKLYEKYLE 77
+WL A + E + A ++L A + +++ K +E +LGNI+ RKL + L+
Sbjct: 217 IWLAAFKLEFENHEPERARMLLAKARERGGTERVWMKSAIVERELGNIEEERKLLNEGLK 396
Query: 78 WALENCYAWSKYA*LERSLSE 98
W LE L+E
Sbjct: 397 QFPSFFKLWLMLGQLEERLAE 459
>TC86389 similar to GP|12322855|gb|AAG51417.1 unknown protein; 78656-75813
{Arabidopsis thaliana}, partial (86%)
Length = 1260
Score = 27.7 bits (60), Expect = 1.3
Identities = 28/111 (25%), Positives = 50/111 (44%), Gaps = 6/111 (5%)
Frame = +1
Query: 24 QFEIRQLNLKG----AHVILGSAIGKAPKDKIL-KKYIEIELQLGNIDRCRKLYEKYLEW 78
+ E L KG A S + P D+I+ K+ + + GNI + KYLE
Sbjct: 421 RLEAMLLEAKGSWEKAEKAYSSLLEDNPLDQIIHKRRVAMAKAQGNISGAIEWLNKYLEI 600
Query: 79 ALENCYAWSKYA*LERSLSETDRARSIF-ELAISQPASDMPELLWKVNIIY 128
+ + AW + A + SL +A + E+ ++QP + L + +++Y
Sbjct: 601 FMADHDAWRELAEIYSSLQMYKQAAFCYEEVILAQPTVPLYHLAY-ADVLY 750
>TC89964 weakly similar to PIR|B96639|B96639 protein T1F9.16 [imported] -
Arabidopsis thaliana, partial (35%)
Length = 928
Score = 27.3 bits (59), Expect = 1.7
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +2
Query: 74 KYLEWALENCYAWSKYA*LERSL 96
K+ +W L++ AWS+Y +ER L
Sbjct: 428 KFAKWQLKHSPAWSRYLEIERGL 496
>BF633350 similar to GP|17473640|gb| unknown protein {Arabidopsis thaliana},
partial (15%)
Length = 513
Score = 26.6 bits (57), Expect = 2.8
Identities = 13/47 (27%), Positives = 26/47 (54%), Gaps = 3/47 (6%)
Frame = -1
Query: 10 SQKFSFANMWLLAAQFEIRQLNLKGAHVILGSA---IGKAPKDKILK 53
S++FSF+ +WL + R L + + ++ SA + KD++L+
Sbjct: 267 SERFSFSALWLASPATSFRSLLCEAINALINSAGD*LKPRRKDRLLR 127
>CA859660 similar to GP|10241917|dbj 34 kDa protein {Hop latent virus},
partial (5%)
Length = 179
Score = 26.2 bits (56), Expect = 3.7
Identities = 15/36 (41%), Positives = 20/36 (54%)
Frame = +2
Query: 38 ILGSAIGKAPKDKILKKYIEIELQLGNIDRCRKLYE 73
++ I KD++LKKYI+ LGN RKL E
Sbjct: 20 VIEKIISSRDKDELLKKYIDAFEDLGN--NYRKLNE 121
>TC87238 similar to PIR|H96781|H96781 unknown protein F22H5.20 [imported] -
Arabidopsis thaliana, partial (57%)
Length = 809
Score = 25.8 bits (55), Expect = 4.8
Identities = 23/77 (29%), Positives = 36/77 (45%), Gaps = 11/77 (14%)
Frame = +2
Query: 13 FSFANMWLLAAQF----EIRQLNLKGAHVILGSAIG-------KAPKDKILKKYIEIELQ 61
F NM+LL Q E L + L +AIG K D L++Y+E +
Sbjct: 227 FFGGNMFLLRRQTQNQPENASLTQEAKVAELRAAIGPLSGRRLKYCTDACLRRYLEA--R 400
Query: 62 LGNIDRCRKLYEKYLEW 78
N+D+ +K+ E+ L+W
Sbjct: 401 NWNVDKAKKMLEESLKW 451
>AW686502 weakly similar to PIR|B84742|B8 probable receptor-like protein
kinase [imported] - Arabidopsis thaliana, partial (9%)
Length = 631
Score = 25.0 bits (53), Expect = 8.2
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +2
Query: 2 QGVSTLDTSQKFSFANMWLLAAQFEI 27
Q +++LD F+ +WLLAA E+
Sbjct: 254 QTITSLDQFLPLKFSKVWLLAALLEV 331
>TC82414 similar to PIR|T49915|T49915 pre-mRNA splicing factor
ATP-dependent RNA helicase-like protein - Arabidopsis
thaliana, partial (13%)
Length = 740
Score = 25.0 bits (53), Expect = 8.2
Identities = 10/28 (35%), Positives = 14/28 (49%)
Frame = -2
Query: 13 FSFANMWLLAAQFEIRQLNLKGAHVILG 40
FS A +W A Q ++ G HV+ G
Sbjct: 85 FSLAELWKYAEQIAFLTTSVSGKHVVKG 2
>BE997694 similar to GP|13646986|db DNA-binding protein DF1 {Pisum sativum},
partial (19%)
Length = 609
Score = 25.0 bits (53), Expect = 8.2
Identities = 9/26 (34%), Positives = 14/26 (53%)
Frame = -2
Query: 64 NIDRCRKLYEKYLEWALENCYAWSKY 89
N+ RCR + +Y +W C W +Y
Sbjct: 446 NLLRCRYCWYRYWQWC---CCCWQRY 378
>TC89377 similar to GP|10177940|dbj|BAB11299. gene_id:MQD19.5~unknown protein
{Arabidopsis thaliana}, partial (66%)
Length = 1480
Score = 25.0 bits (53), Expect = 8.2
Identities = 11/31 (35%), Positives = 18/31 (57%), Gaps = 2/31 (6%)
Frame = -1
Query: 59 ELQLGNIDRCRKLYE--KYLEWALENCYAWS 87
E Q ++D C L E ++ W+L+ C+ WS
Sbjct: 1147 EHQSWSLDYCHDLSEACEHQSWSLDYCHDWS 1055
>BF632466
Length = 579
Score = 25.0 bits (53), Expect = 8.2
Identities = 21/69 (30%), Positives = 32/69 (45%), Gaps = 9/69 (13%)
Frame = -1
Query: 49 DKILKKYIEIELQLGNIDRCRKLYEKYLEWAL-ENCYAWSKYA*LERSL--------SET 99
D K++ + LQL ++D RKL K L E+ W +E + S+T
Sbjct: 324 DDFACKHVSLHLQLRHLDEARKLVAKLCSGKLAESVQLWELRVFIEITCITQSFLLPSDT 145
Query: 100 DRARSIFEL 108
D + S+FEL
Sbjct: 144 DLS-SLFEL 121
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.138 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,980,001
Number of Sequences: 36976
Number of extensions: 44794
Number of successful extensions: 248
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 243
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of query: 128
length of database: 9,014,727
effective HSP length: 85
effective length of query: 43
effective length of database: 5,871,767
effective search space: 252485981
effective search space used: 252485981
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0060.17