Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0058a.4
         (457 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC85128 similar to PIR|D84577|D84577 probable recA protein [impo...    30  1.5
BI265290 similar to SP|P32295|ARG7_P Indole-3-acetic acid induce...    30  1.9
BF637853 weakly similar to GP|15293081|gb| unknown protein {Arab...    30  2.5
BF631741 similar to SP|P32295|ARG7_P Indole-3-acetic acid induce...    30  2.5
TC79586 similar to SP|P52425|GPDA_CUPLA Glycerol-3-phosphate deh...    28  5.6
TC83080 similar to PIR|E86445|E86445 hypothetical protein AAG234...    28  7.4
BM780125                                                               28  7.4
TC86852 similar to GP|15451044|gb|AAK96793.1 Unknown protein {Ar...    28  9.6

>TC85128 similar to PIR|D84577|D84577 probable recA protein [imported] -
           Arabidopsis thaliana, partial (71%)
          Length = 821

 Score = 30.4 bits (67), Expect = 1.5
 Identities = 16/43 (37%), Positives = 24/43 (55%)
 Frame = -1

Query: 200 GNREYYCMLLASWLEEEQVLELLSNLLKAQDLLSAQAKRLQSQ 242
           G  E  C+    WL E+QVL+ L  +  A DL SA +  +++Q
Sbjct: 302 GIHEAKCLFTTIWL*EKQVLKYLXPIDSASDLSSACSASIKAQ 174


>BI265290 similar to SP|P32295|ARG7_P Indole-3-acetic acid induced protein
           ARG7. [Mung bean  Vigna radiata] {Phaseolus aureus},
           partial (67%)
          Length = 531

 Score = 30.0 bits (66), Expect = 1.9
 Identities = 12/39 (30%), Positives = 21/39 (53%)
 Frame = +3

Query: 131 KMYVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISC 169
           K +V+    +    F+D ++K +E+F +H P G   I C
Sbjct: 189 KRFVIPIGCLNQPSFQDLLSKAEEEFGYHHPMGGLTIPC 305


>BF637853 weakly similar to GP|15293081|gb| unknown protein {Arabidopsis
           thaliana}, partial (20%)
          Length = 499

 Score = 29.6 bits (65), Expect = 2.5
 Identities = 20/58 (34%), Positives = 29/58 (49%), Gaps = 2/58 (3%)
 Frame = +1

Query: 173 PHGSRGLIFHGDSI--TGQVKDLWDHYMFGNREYYCMLLASWLEEEQVLELLSNLLKA 228
           P G RGL+ +GD    +GQ  + W   + G    +C L AS LE   +L L+   + A
Sbjct: 244 PDGKRGLVSYGDGSPDSGQR*NSWSPMVLGIVVIFCELGASILELHLLLCLMLEFVDA 417


>BF631741 similar to SP|P32295|ARG7_P Indole-3-acetic acid induced protein
           ARG7. [Mung bean  Vigna radiata] {Phaseolus aureus},
           partial (78%)
          Length = 450

 Score = 29.6 bits (65), Expect = 2.5
 Identities = 12/39 (30%), Positives = 22/39 (55%)
 Frame = +1

Query: 131 KMYVVLQAWIQHWEFKDFVNKRQEQFLWHRPAGNTLISC 169
           K +V+  +++    F+D +N+ +EQF +  P G   I C
Sbjct: 70  KRFVIPISYLNQPLFQDLLNQAEEQFEYDHPTGGLTIPC 186


>TC79586 similar to SP|P52425|GPDA_CUPLA Glycerol-3-phosphate dehydrogenase
           [NAD+] (EC 1.1.1.8). {Cuphea lanceolata}, partial (92%)
          Length = 1521

 Score = 28.5 bits (62), Expect = 5.6
 Identities = 9/24 (37%), Positives = 16/24 (66%)
 Frame = +1

Query: 124 VDFIHLTKMYVVLQAWIQHWEFKD 147
           V FI +   + ++ +W+QHW FK+
Sbjct: 130 VPFIEIFT*FSIVLSWLQHWRFKE 201


>TC83080 similar to PIR|E86445|E86445 hypothetical protein AAG23445.1
           [imported] - Arabidopsis thaliana, partial (11%)
          Length = 1147

 Score = 28.1 bits (61), Expect = 7.4
 Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 6/35 (17%)
 Frame = +3

Query: 148 FVNKRQEQFL---WHRPAGNTLIS---CHFWPHGS 176
           F N    +F+   WH  A N ++S   C FWPH +
Sbjct: 669 FTNMFPSRFI*GHWHNTARNLVLSDSFCFFWPHAT 773


>BM780125 
          Length = 688

 Score = 28.1 bits (61), Expect = 7.4
 Identities = 24/85 (28%), Positives = 40/85 (46%)
 Frame = +3

Query: 221 LLSNLLKAQDLLSAQAKRLQSQLDLLAGHWENPQSQLPKSRPLIEVPKQKSNFDSHGLLK 280
           LLS+  + Q  L+A +   QS  D ++ HW+ P+ Q   + P    PK+K +  +H    
Sbjct: 357 LLSSFEQNQPDLNAVSMESQSTGDRISSHWQ-PKFQASTNNPRGNRPKRKES--AHAAAG 527

Query: 281 VVMTIGMEEYELKKKALSVPQQNQP 305
           V  + G ++ E        P Q+ P
Sbjct: 528 VSFSDG-QDNESGTLTAQPPSQSVP 599


>TC86852 similar to GP|15451044|gb|AAK96793.1 Unknown protein {Arabidopsis
            thaliana}, partial (16%)
          Length = 1861

 Score = 27.7 bits (60), Expect = 9.6
 Identities = 17/34 (50%), Positives = 23/34 (67%)
 Frame = -2

Query: 206  CMLLASWLEEEQVLELLSNLLKAQDLLSAQAKRL 239
            CML  ++ +EEQ LE L  LLK    LSAQ++R+
Sbjct: 1026 CMLTMTFHQEEQHLEPLQWLLK----LSAQSRRM 937


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.320    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,570,697
Number of Sequences: 36976
Number of extensions: 240844
Number of successful extensions: 1049
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1043
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1049
length of query: 457
length of database: 9,014,727
effective HSP length: 99
effective length of query: 358
effective length of database: 5,354,103
effective search space: 1916768874
effective search space used: 1916768874
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)


Lotus: description of TM0058a.4