BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0050.14
(77 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC85936 homologue to GP|13537115|dbj|BAB40755. AtSUG1 {Arabidops... 106 2e-24
TC85744 homologue to PIR|T08959|T08959 proteinase homolog F19B15... 74 8e-15
TC85745 homologue to GP|17297987|dbj|BAB78491. 26S proteasome re... 74 8e-15
TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AA... 73 2e-14
TC85768 homologue to SP|P54774|CC48_SOYBN Cell division cycle pr... 73 2e-14
TC87676 PIR|G96577|G96577 26S proteasome ATPase subunit [importe... 71 9e-14
TC86159 homologue to GP|21387177|gb|AAM47992.1 26S proteasome AA... 69 3e-13
TC77012 homologue to SP|P54776|PRSA_LYCES 26S protease regulator... 67 1e-12
BF647954 homologue to GP|11094192|db 26S proteasome regulatory p... 62 3e-11
TC81332 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22... 60 1e-10
TC88876 similar to PIR|G96537|G96537 hypothetical protein F2J10.... 56 2e-09
TC76904 homologue to GP|4325041|gb|AAD17230.1| FtsH-like protein... 56 2e-09
TC78210 similar to GP|6056413|gb|AAF02877.1| Unknown protein {Ar... 56 3e-09
TC86186 similar to GP|4325041|gb|AAD17230.1| FtsH-like protein P... 55 6e-09
TC86562 similar to GP|21553404|gb|AAM62497.1 26S proteasome regu... 54 8e-09
TC88085 similar to GP|20259341|gb|AAM13995.1 unknown protein {Ar... 54 8e-09
TC87817 homologue to GP|20161224|dbj|BAB90151. katanin p60 subun... 54 1e-08
BF003773 homologue to PIR|T08913|T08 cell division protein FtsH ... 54 1e-08
TC78797 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22... 53 2e-08
AW696218 homologue to PIR|B96835|B96 CAD ATPase (AAA1) 35570-33... 52 6e-08
>TC85936 homologue to GP|13537115|dbj|BAB40755. AtSUG1 {Arabidopsis
thaliana}, partial (98%)
Length = 1633
Score = 106 bits (264), Expect = 2e-24
Identities = 51/56 (91%), Positives = 53/56 (94%)
Frame = +1
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I +VIE+PIKHPELFES GIAQPKGVLLYGPPGT KTLLARAVAHHTDCTFIRVSG
Sbjct: 658 IKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSG 825
>TC85744 homologue to PIR|T08959|T08959 proteinase homolog F19B15.70 -
Arabidopsis thaliana, complete
Length = 1629
Score = 74.3 bits (181), Expect = 8e-15
Identities = 34/56 (60%), Positives = 43/56 (76%)
Frame = +2
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I + +E+P+ HPEL+E GI PKGV+LYG PGT KTLLA+AVA+ T TF+RV G
Sbjct: 689 IKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVG 856
>TC85745 homologue to GP|17297987|dbj|BAB78491. 26S proteasome regulatory
particle triple-A ATPase subunit2b {Oryza sativa
(japonica cultivar-group), partial (98%)
Length = 1696
Score = 74.3 bits (181), Expect = 8e-15
Identities = 34/56 (60%), Positives = 43/56 (76%)
Frame = +3
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
I + +E+P+ HPEL+E GI PKGV+LYG PGT KTLLA+AVA+ T TF+RV G
Sbjct: 687 IKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVG 854
>TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AAA-ATPase
subunit RPT3 {Arabidopsis thaliana}, partial (94%)
Length = 1575
Score = 73.2 bits (178), Expect = 2e-14
Identities = 33/59 (55%), Positives = 44/59 (73%)
Frame = +2
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSGKVY 66
I + +E+P+ H EL++ GI P+GVLLYGPPGT KT+LA+AVA+HT FIRV G +
Sbjct: 611 IREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANHTTAAFIRVVGSEF 787
>TC85768 homologue to SP|P54774|CC48_SOYBN Cell division cycle protein 48
homolog (Valosin containing protein homolog) (VCP).
[Soybean], partial (95%)
Length = 2785
Score = 72.8 bits (177), Expect = 2e-14
Identities = 30/59 (50%), Positives = 47/59 (78%)
Frame = +2
Query: 5 LSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
++ I +++E+P++HP+LF+S G+ PKG+LLYGPPG+ KTL+ARAVA+ T F ++G
Sbjct: 743 MAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGSGKTLIARAVANETGAFFFCING 919
Score = 62.4 bits (150), Expect = 3e-11
Identities = 26/54 (48%), Positives = 37/54 (68%)
Frame = +2
Query: 10 QVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
+ ++ P++HPE FE G++ KGVL YGPPG KTLLA+A+A+ FI + G
Sbjct: 1577 ETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKG 1738
>TC87676 PIR|G96577|G96577 26S proteasome ATPase subunit [imported] -
Arabidopsis thaliana, partial (56%)
Length = 963
Score = 70.9 bits (172), Expect = 9e-14
Identities = 35/51 (68%), Positives = 38/51 (73%)
Frame = +2
Query: 13 EVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
E+P+ HPE F GI PKGVL YGPPGT KTLLARAVA+ TD FIRV G
Sbjct: 2 ELPMLHPEKFVKLGIDPPKGVLCYGPPGTGKTLLARAVANRTDACFIRVIG 154
>TC86159 homologue to GP|21387177|gb|AAM47992.1 26S proteasome AAA-ATPase
subunit RPT4a-like protein {Arabidopsis thaliana},
complete
Length = 1640
Score = 69.3 bits (168), Expect = 3e-13
Identities = 36/78 (46%), Positives = 49/78 (62%), Gaps = 1/78 (1%)
Frame = +2
Query: 1 LASCLSYILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIR 60
L+ + + + IE+P+ +PELF GI PKGVLLYGPPGT KTLLARA+A + D F++
Sbjct: 500 LSDQIRELRESIELPLMNPELFLRVGIKPPKGVLLYGPPGTGKTLLARAIASNIDANFLK 679
Query: 61 -VSGKVYSLYSASSTHYL 77
VS + Y S +
Sbjct: 680 VVSSAIIDKYIGESARLI 733
>TC77012 homologue to SP|P54776|PRSA_LYCES 26S protease regulatory subunit
6A homolog (TAT-binding protein homolog 1) (TBP-1)
(Mg(2+), complete
Length = 1793
Score = 67.4 bits (163), Expect = 1e-12
Identities = 30/56 (53%), Positives = 42/56 (74%)
Frame = +1
Query: 8 ILQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
+++ I +P+ H E F+ GI PKGVLLYGPPGT KTL+ARA A T+ TF++++G
Sbjct: 676 LVEAIVLPMTHKERFQKLGIRPPKGVLLYGPPGTGKTLMARACAAQTNATFLKLAG 843
>BF647954 homologue to GP|11094192|db 26S proteasome regulatory particle
triple-A ATPase subunit4 {Oryza sativa (japonica
cultivar-group)}, partial (53%)
Length = 649
Score = 62.4 bits (150), Expect = 3e-11
Identities = 32/61 (52%), Positives = 40/61 (65%), Gaps = 1/61 (1%)
Frame = +2
Query: 18 HPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIR-VSGKVYSLYSASSTHY 76
+PELF GI PKGVLLYGPPGT KTLLARA+A + D F++ VS + Y S+
Sbjct: 5 NPELFLRVGIKPPKGVLLYGPPGTGKTLLARAIASNIDANFLKVVSSAIIDKYIGESSRL 184
Query: 77 L 77
+
Sbjct: 185I 187
>TC81332 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22
{Arabidopsis thaliana}, partial (39%)
Length = 1514
Score = 60.5 bits (145), Expect = 1e-10
Identities = 26/54 (48%), Positives = 41/54 (75%), Gaps = 1/54 (1%)
Frame = +1
Query: 10 QVIEVPIKHPELFESPGIAQP-KGVLLYGPPGTRKTLLARAVAHHTDCTFIRVS 62
+ + +P++ P+LF+ G+ +P KGVLL+GPPGT KT+LA+A+A+ +FI VS
Sbjct: 880 EAVMLPLRRPDLFKGDGVLKPCKGVLLFGPPGTGKTMLAKAIANEAGASFINVS 1041
>TC88876 similar to PIR|G96537|G96537 hypothetical protein F2J10.1 [imported]
- Arabidopsis thaliana, partial (72%)
Length = 1572
Score = 56.2 bits (134), Expect = 2e-09
Identities = 26/55 (47%), Positives = 39/55 (70%), Gaps = 1/55 (1%)
Frame = +2
Query: 10 QVIEVPIKHPELFESPGIAQP-KGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSG 63
+++ +P++ PELF + +P KG+LL+GPPGT KTLLA+A+A FI V+G
Sbjct: 962 ELVILPMRRPELFSHGNLLRPCKGILLFGPPGTGKTLLAKALATEAGANFISVTG 1126
>TC76904 homologue to GP|4325041|gb|AAD17230.1| FtsH-like protein Pftf
precursor {Nicotiana tabacum}, partial (91%)
Length = 2667
Score = 56.2 bits (134), Expect = 2e-09
Identities = 29/58 (50%), Positives = 37/58 (63%)
Frame = +1
Query: 9 LQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSGKVY 66
++V+E +K PE F S G PKGVLL GPPGT KTLLA+A+A F +SG +
Sbjct: 868 MEVVEF-LKKPERFTSVGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISGSEF 1038
>TC78210 similar to GP|6056413|gb|AAF02877.1| Unknown protein {Arabidopsis
thaliana}, partial (23%)
Length = 1254
Score = 55.8 bits (133), Expect = 3e-09
Identities = 26/54 (48%), Positives = 38/54 (70%), Gaps = 1/54 (1%)
Frame = +3
Query: 10 QVIEVPIKHPELFESPGIAQP-KGVLLYGPPGTRKTLLARAVAHHTDCTFIRVS 62
+++ +P+K PELF + +P KG+LL+GPPGT KT+LA+AVA FI +S
Sbjct: 6 ELVMLPLKRPELFCKGQLTKPCKGILLFGPPGTGKTMLAKAVATEAGANFINIS 167
>TC86186 similar to GP|4325041|gb|AAD17230.1| FtsH-like protein Pftf
precursor {Nicotiana tabacum}, partial (47%)
Length = 1303
Score = 54.7 bits (130), Expect = 6e-09
Identities = 28/58 (48%), Positives = 37/58 (63%)
Frame = +2
Query: 9 LQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSGKVY 66
++V+E +K PE F + G PKGVLL GPPGT KTLLA+A+A F +SG +
Sbjct: 893 MEVVEF-LKKPERFTAIGARIPKGVLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEF 1063
>TC86562 similar to GP|21553404|gb|AAM62497.1 26S proteasome regulatory
particle chain RPT6-like protein {Arabidopsis thaliana},
partial (77%)
Length = 1564
Score = 54.3 bits (129), Expect = 8e-09
Identities = 25/55 (45%), Positives = 38/55 (68%), Gaps = 1/55 (1%)
Frame = +1
Query: 8 ILQVIEVPIKHPELFESPGIAQP-KGVLLYGPPGTRKTLLARAVAHHTDCTFIRV 61
+ +++ +P++ P+LF + P KGVLLYGPPGT KT+LA+A+A + FI V
Sbjct: 442 LFELVILPLQRPDLFSHGKLLGPQKGVLLYGPPGTGKTMLAKAIAKESGAVFINV 606
>TC88085 similar to GP|20259341|gb|AAM13995.1 unknown protein {Arabidopsis
thaliana}, partial (29%)
Length = 1261
Score = 54.3 bits (129), Expect = 8e-09
Identities = 25/54 (46%), Positives = 38/54 (70%), Gaps = 1/54 (1%)
Frame = +1
Query: 10 QVIEVPIKHPELFESPGIAQP-KGVLLYGPPGTRKTLLARAVAHHTDCTFIRVS 62
+++ +P++ PELF + +P KG+LL+GPPGT KT+LA+AVA FI +S
Sbjct: 805 ELVMLPLQRPELFCKGQLTKPCKGILLFGPPGTGKTMLAKAVATEAGANFINIS 966
>TC87817 homologue to GP|20161224|dbj|BAB90151. katanin p60 subunit A 1-like
{Oryza sativa (japonica cultivar-group)}, partial (25%)
Length = 768
Score = 53.9 bits (128), Expect = 1e-08
Identities = 27/58 (46%), Positives = 39/58 (66%), Gaps = 1/58 (1%)
Frame = +2
Query: 10 QVIEVPIKHPELFESPGIAQP-KGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSGKVY 66
+ + +PIK+P+ F G+ P KG+LL+GPPGT KT+LA+AVA + TF + VY
Sbjct: 539 EAVVMPIKYPKYFT--GLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFFNIFSIVY 706
>BF003773 homologue to PIR|T08913|T08 cell division protein FtsH homolog
T32A16.110 - Arabidopsis thaliana, partial (12%)
Length = 463
Score = 53.5 bits (127), Expect = 1e-08
Identities = 26/58 (44%), Positives = 37/58 (62%)
Frame = +2
Query: 9 LQVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVSGKVY 66
LQ + +K+PELF+ GI P GVLL GPPG KTL+A+A+A F +++G +
Sbjct: 242 LQELVKYLKNPELFDKMGIKPPHGVLLEGPPGCGKTLVAKAIAGEAGVPFYQMAGSEF 415
>TC78797 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22 {Arabidopsis
thaliana}, partial (74%)
Length = 2270
Score = 53.1 bits (126), Expect = 2e-08
Identities = 22/53 (41%), Positives = 37/53 (69%)
Frame = +1
Query: 10 QVIEVPIKHPELFESPGIAQPKGVLLYGPPGTRKTLLARAVAHHTDCTFIRVS 62
+++ +P++ P+LF + +G+LL+GPPGT KT+LA+A+A +FI VS
Sbjct: 1756 ELVMLPLRRPDLFTGGLLKPCRGILLFGPPGTGKTMLAKAIAKEAGASFINVS 1914
>AW696218 homologue to PIR|B96835|B96 CAD ATPase (AAA1) 35570-33019
[imported] - Arabidopsis thaliana, partial (34%)
Length = 556
Score = 51.6 bits (122), Expect = 6e-08
Identities = 28/54 (51%), Positives = 37/54 (67%), Gaps = 1/54 (1%)
Frame = +3
Query: 10 QVIEVPIKHPELFESPGIAQP-KGVLLYGPPGTRKTLLARAVAHHTDCTFIRVS 62
+ + +P+ PE F+ GI +P KGVL++GPPGT KTLLA+AVA TF VS
Sbjct: 93 EAVVLPLWMPEYFQ--GIRRPWKGVLMFGPPGTGKTLLAKAVATECGTTFFNVS 248
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.138 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,632,580
Number of Sequences: 36976
Number of extensions: 27891
Number of successful extensions: 149
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of query: 77
length of database: 9,014,727
effective HSP length: 53
effective length of query: 24
effective length of database: 7,054,999
effective search space: 169319976
effective search space used: 169319976
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0050.14