BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0048.5
(117 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ154200 similar to GP|14488090|gb|A AT5g26741 {Arabidopsis thal... 144 6e-36
BQ135450 29 0.23
TC88072 similar to PIR|T06580|T06580 subtilisin-like proteinase ... 28 0.51
TC80584 similar to PIR|T45688|T45688 hypothetical protein F18L15... 28 0.67
TC87735 similar to GP|9757814|dbj|BAB08332.1 gene_id:MWD9.13~unk... 27 1.1
TC77567 similar to PIR|S56719|S56719 serine/threonine-specific p... 27 1.1
TC87465 similar to PIR|T01602|T01602 probable thiamin pyrophosph... 27 1.1
TC88005 27 1.5
TC80370 similar to GP|21554331|gb|AAM63438.1 unknown {Arabidopsi... 27 1.5
BQ138663 similar to GP|17982445|gb| SODIUM-DEPENDENT PHOSPHATE T... 26 2.0
TC86467 similar to GP|15294264|gb|AAK95309.1 AT4g26670/F10M23_10... 26 2.0
TC90559 similar to PIR|T43291|T43291 laminin alpha chain - Caeno... 26 2.6
TC92376 26 2.6
TC86014 weakly similar to PIR|A96697|A96697 protein F1N21.18 [im... 25 3.3
TC93130 weakly similar to GP|16930483|gb|AAL31927.1 AT3g51670/T1... 25 3.3
TC79709 similar to SP|Q39242|TRB2_ARATH Thioredoxin reductase 2 ... 25 3.3
BG587258 homologue to PIR|T50532|T5053 ribosomal protein S27 - A... 25 3.3
AJ500959 similar to GP|17065042|gb Unknown protein {Arabidopsis ... 25 4.4
TC79007 similar to GP|20260516|gb|AAM13156.1 unknown protein {Ar... 25 4.4
TC77888 homologue to PIR|E84758|E84758 probable pyruvate dehydro... 25 4.4
>BQ154200 similar to GP|14488090|gb|A AT5g26741 {Arabidopsis thaliana},
partial (29%)
Length = 464
Score = 144 bits (362), Expect = 6e-36
Identities = 75/108 (69%), Positives = 86/108 (79%), Gaps = 4/108 (3%)
Frame = +1
Query: 14 ESLGHYYDYNYYPDQNYTMMEKRQLFLRSYQFCQKKSFTERIKGSLIRAKKVLWLRLWSS 73
ES G+Y +PDQNYTM+EKRQLFLRSYQFC+KKS TERIKGSL+RAKKV+WL+L
Sbjct: 91 ESFGYYN----FPDQNYTMIEKRQLFLRSYQFCRKKSLTERIKGSLVRAKKVVWLKL-RY 255
Query: 74 SAGGFRKLVFFPRFRCTFYYRRRRFSRLLNR----HHRSKTESSSCFW 117
+ G RKLVFFPRF+C FYYRRRRFS+LLN HHR K +SSSC W
Sbjct: 256 ARGLRRKLVFFPRFKCGFYYRRRRFSQLLNNSGSSHHR-KVDSSSCLW 396
>BQ135450
Length = 759
Score = 29.3 bits (64), Expect = 0.23
Identities = 17/54 (31%), Positives = 27/54 (49%), Gaps = 5/54 (9%)
Frame = +1
Query: 4 VTVNPSLFRSESLGHYYDYN-----YYPDQNYTMMEKRQLFLRSYQFCQKKSFT 52
+T N SLF + + HYY+ Y+ +NY+ + +R +YQ K S T
Sbjct: 52 ITKN*SLFSTIPIQHYYNLTILCPCYHHSKNYSTIYQRNSLPNTYQNLDKLSTT 213
>TC88072 similar to PIR|T06580|T06580 subtilisin-like proteinase (EC
3.4.21.-) p69f - tomato, partial (27%)
Length = 1097
Score = 28.1 bits (61), Expect = 0.51
Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 3/36 (8%)
Frame = +1
Query: 83 FFPRFRCTFYYRRRRFSRLLN---RHHRSKTESSSC 115
FF F C +++R + + N RHH S+ + SSC
Sbjct: 130 FFSSFCCLLFFKRTKPRKSRNP*TRHHWSRCQHSSC 237
>TC80584 similar to PIR|T45688|T45688 hypothetical protein F18L15.30 -
Arabidopsis thaliana, partial (15%)
Length = 714
Score = 27.7 bits (60), Expect = 0.67
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 8/52 (15%)
Frame = +2
Query: 23 NYYPDQNYTMMEKRQLFLRSYQFCQKK-SFTER-------IKGSLIRAKKVL 66
NY + RQ++LRSY+F +KK S TE+ +K S+I A +L
Sbjct: 167 NYRDRVEVDSLSCRQVYLRSYKFSRKKLSVTEKTIKYFSSLKESVICASNIL 322
>TC87735 similar to GP|9757814|dbj|BAB08332.1 gene_id:MWD9.13~unknown
protein {Arabidopsis thaliana}, partial (48%)
Length = 1211
Score = 26.9 bits (58), Expect = 1.1
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = +3
Query: 6 VNPSLFRSESLGHYYDYNYYPD 27
++P F ++S +YY YNYY +
Sbjct: 159 IHPLCFITKSFSYYYCYNYYSE 224
>TC77567 similar to PIR|S56719|S56719 serine/threonine-specific protein
kinase SPK-1 (EC 2.7.1.-) - soybean, partial (64%)
Length = 744
Score = 26.9 bits (58), Expect = 1.1
Identities = 15/35 (42%), Positives = 23/35 (64%), Gaps = 4/35 (11%)
Frame = +1
Query: 26 PDQNY-TMMEKRQLFLRS---YQFCQKKSFTERIK 56
P Q Y T+ +++QL LR +FC K+S TER++
Sbjct: 487 PSQQYCTLNQRQQLDLRHTLLLRFCYKRSTTERLQ 591
>TC87465 similar to PIR|T01602|T01602 probable thiamin pyrophosphokinase
At2g44750 [imported] - Arabidopsis thaliana, partial
(88%)
Length = 1097
Score = 26.9 bits (58), Expect = 1.1
Identities = 10/40 (25%), Positives = 24/40 (60%)
Frame = +2
Query: 54 RIKGSLIRAKKVLWLRLWSSSAGGFRKLVFFPRFRCTFYY 93
R+KGS +V++ ++ + G++++ + P RC F++
Sbjct: 977 RVKGSYSLIWEVIFPITNNNESSGYKEVTYIP*LRCMFFF 1096
>TC88005
Length = 1122
Score = 26.6 bits (57), Expect = 1.5
Identities = 18/53 (33%), Positives = 20/53 (36%), Gaps = 4/53 (7%)
Frame = +3
Query: 69 RLWSSSAGGFRKLVFFPRFRCTFYYRRRR----FSRLLNRHHRSKTESSSCFW 117
R S AGG L F P F R F + HH SK + S FW
Sbjct: 747 RYSSLYAGGDVVLAFLPHVGLLFLSPLREAFGSFGHRRDGHHHSKAQEHSLFW 905
>TC80370 similar to GP|21554331|gb|AAM63438.1 unknown {Arabidopsis
thaliana}, partial (32%)
Length = 808
Score = 26.6 bits (57), Expect = 1.5
Identities = 14/30 (46%), Positives = 18/30 (59%), Gaps = 2/30 (6%)
Frame = +1
Query: 36 RQLFLRSYQFCQ--KKSFTERIKGSLIRAK 63
RQL+LRSY FC+ +K E+ K R K
Sbjct: 478 RQLYLRSYTFCRDDEKKVLEKTKKCFGRVK 567
>BQ138663 similar to GP|17982445|gb| SODIUM-DEPENDENT PHOSPHATE TRANSPORT
PROTEIN {Brucella melitensis}, partial (7%)
Length = 693
Score = 26.2 bits (56), Expect = 2.0
Identities = 8/24 (33%), Positives = 16/24 (66%)
Frame = -1
Query: 12 RSESLGHYYDYNYYPDQNYTMMEK 35
R++S Y ++Y+P QN++ + K
Sbjct: 687 RAQSSSSLYQWSYFPXQNFSFLSK 616
>TC86467 similar to GP|15294264|gb|AAK95309.1 AT4g26670/F10M23_10
{Arabidopsis thaliana}, partial (57%)
Length = 967
Score = 26.2 bits (56), Expect = 2.0
Identities = 15/56 (26%), Positives = 26/56 (45%)
Frame = -3
Query: 13 SESLGHYYDYNYYPDQNYTMMEKRQLFLRSYQFCQKKSFTERIKGSLIRAKKVLWL 68
S S+ HY + + R R +Q QKK +++G+ I+AK+ +L
Sbjct: 851 SSSINHYISFQVQTIRREKNTSPRITMFRHHQVLQKKKC**KLQGT*IKAKEGAYL 684
>TC90559 similar to PIR|T43291|T43291 laminin alpha chain - Caenorhabditis
elegans, partial (1%)
Length = 920
Score = 25.8 bits (55), Expect = 2.6
Identities = 14/46 (30%), Positives = 26/46 (56%)
Frame = -1
Query: 58 SLIRAKKVLWLRLWSSSAGGFRKLVFFPRFRCTFYYRRRRFSRLLN 103
SL+ ++ ++ +++ F VFF F +F + R +FSR+LN
Sbjct: 290 SLMNSRLIIPPLMYTCYLTSFVLGVFFVGFSFSFSFSRVKFSRILN 153
>TC92376
Length = 274
Score = 25.8 bits (55), Expect = 2.6
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1
Query: 78 FRKLVFFPRFRCTFYYRRRRFS 99
FR + F+ FRC F++ RFS
Sbjct: 193 FRVVCFYRFFRCCFFFLTTRFS 128
>TC86014 weakly similar to PIR|A96697|A96697 protein F1N21.18 [imported] -
Arabidopsis thaliana, partial (51%)
Length = 1092
Score = 25.4 bits (54), Expect = 3.3
Identities = 16/34 (47%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = -2
Query: 84 FPRFRCTFYYRRRRFSRLLNRHHRSK-TESSSCF 116
FPRF C F + F R L + RS TE SCF
Sbjct: 545 FPRFMCYFLNKIFGFVRKLLSNMRSMLTELVSCF 444
>TC93130 weakly similar to GP|16930483|gb|AAL31927.1 AT3g51670/T18N14_50
{Arabidopsis thaliana}, partial (16%)
Length = 661
Score = 25.4 bits (54), Expect = 3.3
Identities = 12/41 (29%), Positives = 22/41 (53%), Gaps = 4/41 (9%)
Frame = +2
Query: 39 FLRSYQFCQKKSFTERIKGSLIRAK----KVLWLRLWSSSA 75
FLR+ F ++T +K + K K+LW+++W S +
Sbjct: 449 FLRARDFRVNDAYTMLVKYVFLGEKNLVLKMLWMKIWDSKS 571
>TC79709 similar to SP|Q39242|TRB2_ARATH Thioredoxin reductase 2 (EC
1.6.4.5) (NADPH-dependent thioredoxin reductase 2) (NTR
2)., partial (52%)
Length = 1121
Score = 25.4 bits (54), Expect = 3.3
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +2
Query: 92 YYRRRRFSRLLNRHHRSKTESS 113
Y RRR F RL RH R +T +
Sbjct: 569 YNRRREFPRLPRRHPRRRTHGT 634
>BG587258 homologue to PIR|T50532|T5053 ribosomal protein S27 - Arabidopsis
thaliana, partial (98%)
Length = 663
Score = 25.4 bits (54), Expect = 3.3
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -3
Query: 70 LWSSSAGGFRKLVFFPRFRC 89
L+SSSAGGF++ + F R C
Sbjct: 85 LFSSSAGGFKRSMSFWRTHC 26
>AJ500959 similar to GP|17065042|gb Unknown protein {Arabidopsis thaliana},
partial (38%)
Length = 717
Score = 25.0 bits (53), Expect = 4.4
Identities = 16/45 (35%), Positives = 20/45 (43%)
Frame = +2
Query: 70 LWSSSAGGFRKLVFFPRFRCTFYYRRRRFSRLLNRHHRSKTESSS 114
LW S G L F + RRR S LN +R K++S S
Sbjct: 2 LWPGS*VGKMNLAFNLNLSSSLCIHRRRRSHTLNNVNRRKSKSIS 136
>TC79007 similar to GP|20260516|gb|AAM13156.1 unknown protein {Arabidopsis
thaliana}, partial (96%)
Length = 1561
Score = 25.0 bits (53), Expect = 4.4
Identities = 12/39 (30%), Positives = 17/39 (42%)
Frame = +1
Query: 62 AKKVLWLRLWSSSAGGFRKLVFFPRFRCTFYYRRRRFSR 100
A ++W R+WS +G RC YR +F R
Sbjct: 100 ASCIIWYRIWSQISG*M-------HIRCESRYRSHKFPR 195
>TC77888 homologue to PIR|E84758|E84758 probable pyruvate dehydrogenase E1
beta subunit [imported] - Arabidopsis thaliana, partial
(85%)
Length = 1707
Score = 25.0 bits (53), Expect = 4.4
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = -3
Query: 71 WSSSAGGFRKLVFFPRFRC 89
+ S GF+K F PRFRC
Sbjct: 466 FQSFTQGFKKEKFMPRFRC 410
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.329 0.138 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,123,152
Number of Sequences: 36976
Number of extensions: 80427
Number of successful extensions: 668
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 665
length of query: 117
length of database: 9,014,727
effective HSP length: 93
effective length of query: 24
effective length of database: 5,575,959
effective search space: 133823016
effective search space used: 133823016
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0048.5