BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0048.13
(98 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90655 homologue to GP|17979539|gb|AAL50104.1 At1g65700/F1E22_3... 89 1e-22
TC87426 homologue to PIR|S22826|S22826 small nuclear ribonucleop... 49 4e-07
AJ501228 homologue to SP|O82221|RUXG_A Probable small nuclear ri... 49 4e-07
TC79964 homologue to PIR|B84453|B84453 probable snRNP splicing f... 47 1e-06
TC84710 homologue to PIR|B84453|B84453 probable snRNP splicing f... 43 2e-05
TC78297 homologue to GP|9758886|dbj|BAB09440.1 U6 snRNA-associat... 40 1e-04
TC83940 homologue to PIR|B84453|B84453 probable snRNP splicing f... 37 0.001
TC86384 similar to GP|21593343|gb|AAM65292.1 small nuclear ribon... 33 0.018
TC86383 similar to GP|21593343|gb|AAM65292.1 small nuclear ribon... 33 0.018
TC82136 similar to GP|22202729|dbj|BAC07386. putative snRNP prot... 31 0.068
CA921199 similar to PIR|T51950|T51 DNA-directed RNA polymerase (... 31 0.068
TC88753 similar to PIR|T10586|T10586 small nuclear ribonucleopro... 31 0.068
BQ152647 homologue to PIR|B84453|B8445 probable snRNP splicing f... 25 3.8
TC84896 similar to GP|7596769|gb|AAF64540.1| unknown protein {Ar... 25 6.4
TC80361 weakly similar to PIR|G96818|G96818 F9K20.3 [imported] -... 25 6.4
BE325533 similar to GP|6573766|gb|A F28K19.23 {Arabidopsis thali... 24 8.4
TC85449 homologue to PIR|S71923|S71923 cysteine proteinase (EC 3... 24 8.4
BG586263 SP|P29388|NU5 NADH-ubiquinone oxidoreductase chain 5 (E... 24 8.4
>TC90655 homologue to GP|17979539|gb|AAL50104.1 At1g65700/F1E22_3
{Arabidopsis thaliana}, partial (75%)
Length = 474
Score = 89.4 bits (220), Expect(2) = 1e-22
Identities = 46/65 (70%), Positives = 53/65 (80%)
Frame = +3
Query: 30 KGFDQATNIILDESHERVFSTKEGVQLIALGLYIIRGDNISVVGELDEDLDKNLDKSKIR 89
K +ATNIILDESHERVFST+EGVQ + LGLYIIRGDNISVVGELDE+LD +L S+
Sbjct: 30 KVLTRATNIILDESHERVFSTREGVQQLVLGLYIIRGDNISVVGELDEELDSSLHLSQPY 209
Query: 90 AQPLK 94
+ LK
Sbjct: 210SSSLK 224
Score = 31.6 bits (70), Expect(2) = 1e-22
Identities = 13/15 (86%), Positives = 14/15 (92%)
Frame = +2
Query: 21 DGRNIVGTLKGFDQA 35
DGRNIVG LKGFDQ+
Sbjct: 2 DGRNIVGVLKGFDQS 46
>TC87426 homologue to PIR|S22826|S22826 small nuclear ribonucleoprotein E
homolog - alfalfa, complete
Length = 609
Score = 48.5 bits (114), Expect = 4e-07
Identities = 25/72 (34%), Positives = 43/72 (59%)
Frame = +2
Query: 5 PGLESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGVQLIALGLYII 64
P L+ +D+Q+ + N R IVGTL+GFDQ N+++D + E G + +G+ +I
Sbjct: 92 PDLKKYMDKQLQIKLNANRMIVGTLRGFDQFMNLVVDNTVE-----VNGNEKNDIGMVVI 256
Query: 65 RGDNISVVGELD 76
RG+++ V L+
Sbjct: 257 RGNSVVTVEALE 292
>AJ501228 homologue to SP|O82221|RUXG_A Probable small nuclear
ribonucleoprotein G (snRNP-G) (Sm protein G) (Sm-G)
(SmG). [Mouse-ear cress], partial (98%)
Length = 658
Score = 48.5 bits (114), Expect = 4e-07
Identities = 25/72 (34%), Positives = 43/72 (59%)
Frame = +2
Query: 5 PGLESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGVQLIALGLYII 64
P L+ +D+Q+ + N R IVGTL+GFDQ N+++D + E G + +G+ +I
Sbjct: 212 PDLKKYMDKQLQIKLNANRMIVGTLRGFDQFMNLVVDNTVE-----VNGNEKNDIGMVVI 376
Query: 65 RGDNISVVGELD 76
RG+++ V L+
Sbjct: 377 RGNSVVTVEALE 412
>TC79964 homologue to PIR|B84453|B84453 probable snRNP splicing factor
[imported] - Arabidopsis thaliana, partial (93%)
Length = 607
Score = 47.0 bits (110), Expect = 1e-06
Identities = 26/74 (35%), Positives = 40/74 (53%), Gaps = 4/74 (5%)
Frame = +1
Query: 7 LESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGV----QLIALGLY 62
L VD+ + V GR + GTLKG+DQ N++LDE+ E + + + Q +LGL
Sbjct: 94 LAKFVDKGVQVKLTGGRQVTGTLKGYDQLLNLVLDEAVEFLRDPDDPLKTTDQTRSLGLI 273
Query: 63 IIRGDNISVVGELD 76
+ RG + +V D
Sbjct: 274 VCRGTAVMLVSPTD 315
>TC84710 homologue to PIR|B84453|B84453 probable snRNP splicing factor
[imported] - Arabidopsis thaliana, partial (93%)
Length = 543
Score = 43.1 bits (100), Expect = 2e-05
Identities = 26/74 (35%), Positives = 38/74 (51%), Gaps = 4/74 (5%)
Frame = +3
Query: 7 LESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGV----QLIALGLY 62
L VD+ + V GR + GTLKG+DQ N+ LDE+ E + + + Q +LGL
Sbjct: 48 LAKFVDKGVQVKLTGGRQVTGTLKGYDQLLNLFLDEAVEFLRDPDDPLKTTDQTRSLGLI 227
Query: 63 IIRGDNISVVGELD 76
RG + +V D
Sbjct: 228 GCRGTAVMLVSPTD 269
>TC78297 homologue to GP|9758886|dbj|BAB09440.1 U6 snRNA-associated Sm-like
protein-like {Arabidopsis thaliana}, partial (95%)
Length = 693
Score = 40.0 bits (92), Expect = 1e-04
Identities = 20/66 (30%), Positives = 39/66 (58%)
Frame = +1
Query: 7 LESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGVQLIALGLYIIRG 66
++ + +I VI + +VGTL+GFD N++L++ E T EG ++ L ++ G
Sbjct: 205 IDRCIGSKIWVIMKGDKELVGTLRGFDVYVNMVLEDVTEYEI-TAEGRRITKLDQILLNG 381
Query: 67 DNISVV 72
+NI+++
Sbjct: 382 NNIAIL 399
>TC83940 homologue to PIR|B84453|B84453 probable snRNP splicing factor
[imported] - Arabidopsis thaliana, partial (93%)
Length = 632
Score = 37.0 bits (84), Expect = 0.001
Identities = 20/56 (35%), Positives = 32/56 (56%), Gaps = 4/56 (7%)
Frame = +1
Query: 25 IVGTLKGFDQATNIILDESHERVFSTKEGV----QLIALGLYIIRGDNISVVGELD 76
+ GTLKG+DQ N++LDE+ E + + + Q +LGL + RG + +V D
Sbjct: 235 LTGTLKGYDQLLNLVLDEAVEFLRDPDDPLKTTDQTRSLGLIVCRGTAVMLVSPTD 402
>TC86384 similar to GP|21593343|gb|AAM65292.1 small nuclear
ribonucleoprotein homolog {Arabidopsis thaliana},
complete
Length = 658
Score = 33.1 bits (74), Expect = 0.018
Identities = 20/66 (30%), Positives = 34/66 (51%)
Frame = +1
Query: 7 LESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGVQLIALGLYIIRG 66
L+S QI + I G + GFD+ N++LD++ E K LG +++G
Sbjct: 169 LQSKARIQIWLFEQKDLRIEGRIIGFDEYMNLVLDDAEEVNVKKKSKK---TLGRILLKG 339
Query: 67 DNISVV 72
DNI+++
Sbjct: 340 DNITLM 357
>TC86383 similar to GP|21593343|gb|AAM65292.1 small nuclear
ribonucleoprotein homolog {Arabidopsis thaliana},
complete
Length = 684
Score = 33.1 bits (74), Expect = 0.018
Identities = 20/66 (30%), Positives = 34/66 (51%)
Frame = +2
Query: 7 LESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGVQLIALGLYIIRG 66
L+S QI + I G + GFD+ N++LD++ E K LG +++G
Sbjct: 137 LQSKARIQIWLFEQKDLRIEGRIIGFDEYMNLVLDDAEEVNVKKKSKK---TLGRILLKG 307
Query: 67 DNISVV 72
DNI+++
Sbjct: 308 DNITLM 325
>TC82136 similar to GP|22202729|dbj|BAC07386. putative snRNP protein {Oryza
sativa (japonica cultivar-group)}, partial (51%)
Length = 629
Score = 31.2 bits (69), Expect = 0.068
Identities = 20/78 (25%), Positives = 37/78 (46%), Gaps = 9/78 (11%)
Frame = +2
Query: 1 MSTGPGLESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHE-------RVFSTKEG 53
MS + ++ ++ V DGR +VG FD+ N++L + E + T +G
Sbjct: 29 MSKSSKMLQYINYRMRVTIQDGRQLVGKFMAFDRHMNLVLGDWEEFRKLPPVKGKKTTDG 208
Query: 54 --VQLIALGLYIIRGDNI 69
+ LGL ++RG+ +
Sbjct: 209 DREERRTLGLLLLRGEEV 262
>CA921199 similar to PIR|T51950|T51 DNA-directed RNA polymerase (EC 2.7.7.6)
23K chain [imported] - Arabidopsis thaliana, partial
(49%)
Length = 756
Score = 31.2 bits (69), Expect = 0.068
Identities = 13/39 (33%), Positives = 24/39 (61%)
Frame = +2
Query: 7 LESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHE 45
++ + +I VI + +VGTL+GFD N++L++ E
Sbjct: 617 IDRCIGSKIWVIMKGDKELVGTLRGFDVYVNMVLEDVTE 733
>TC88753 similar to PIR|T10586|T10586 small nuclear
ribonucleoprotein-associated protein homolog F9F13.90 -
Arabidopsis thaliana, partial (77%)
Length = 1273
Score = 31.2 bits (69), Expect = 0.068
Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 9/68 (13%)
Frame = +2
Query: 11 VDQQISVITNDGRNIVGTLKGFDQATNIILDESHE-RVFSTKEGVQLI--------ALGL 61
++ ++ V DGR +VG FD+ N++L + E R +G + LGL
Sbjct: 158 INYRMRVTIQDGRQLVGKFMAFDRHMNLVLGDCEEFRKLPPAKGKKTTDGDREDRRTLGL 337
Query: 62 YIIRGDNI 69
++RG+ +
Sbjct: 338 VLLRGEEV 361
>BQ152647 homologue to PIR|B84453|B8445 probable snRNP splicing factor
[imported] - Arabidopsis thaliana, partial (73%)
Length = 495
Score = 25.4 bits (54), Expect = 3.8
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +3
Query: 22 GRNIVGTLKGFDQ 34
GR + GTLKG+DQ
Sbjct: 48 GRQVTGTLKGYDQ 86
>TC84896 similar to GP|7596769|gb|AAF64540.1| unknown protein {Arabidopsis
thaliana}, partial (16%)
Length = 786
Score = 24.6 bits (52), Expect = 6.4
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 65 RGDNISVVGELDEDLDKNLDKSKIRAQPLK 94
+GD ISV+ E+L+ +L K K+ LK
Sbjct: 139 KGDLISVLKAEREELESSLSKEKLHTLQLK 228
>TC80361 weakly similar to PIR|G96818|G96818 F9K20.3 [imported] -
Arabidopsis thaliana, partial (33%)
Length = 847
Score = 24.6 bits (52), Expect = 6.4
Identities = 20/71 (28%), Positives = 36/71 (50%), Gaps = 14/71 (19%)
Frame = +3
Query: 33 DQATNIILDESHERV--FSTKEGVQL--------IALGLYIIRG----DNISVVGELDED 78
D + I + ES + F+TK G+ + ALG ++ G DNI++V + D
Sbjct: 357 DYDSPISISESDNKTISFATKVGIGVAVPVFALVFALGDFLPSGSPNEDNIALVNDKLSD 536
Query: 79 LDKNLDKSKIR 89
+DK +S+++
Sbjct: 537 MDKTALQSRLK 569
>BE325533 similar to GP|6573766|gb|A F28K19.23 {Arabidopsis thaliana},
partial (14%)
Length = 525
Score = 24.3 bits (51), Expect = 8.4
Identities = 14/70 (20%), Positives = 33/70 (47%), Gaps = 1/70 (1%)
Frame = +1
Query: 7 LESLVDQQISVITNDGRNIVGTLKGFDQATNIILDESHERVFSTKEGVQLIALGLYIIRG 66
+ S + +VITN ++ ++ + TN++ + + T G A+G+ + G
Sbjct: 139 ISSSTNYSYNVITNKDERLLEQIENDNNNTNVVANSTTIAAIVTSLGGPPAAVGIVRLSG 318
Query: 67 DN-ISVVGEL 75
+ +S+ G +
Sbjct: 319 PHAVSIAGRV 348
>TC85449 homologue to PIR|S71923|S71923 cysteine proteinase (EC 3.4.22.-) -
garden pea, complete
Length = 1534
Score = 24.3 bits (51), Expect = 8.4
Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 5/37 (13%)
Frame = -1
Query: 40 LDESHERVFSTKEGVQLIALGLYIIRG-----DNISV 71
L E HE + + K +++ YIIRG DN+ V
Sbjct: 1447 LKEGHELLSNDKHTPSMLSFNCYIIRGNTPNKDNVCV 1337
>BG586263 SP|P29388|NU5 NADH-ubiquinone oxidoreductase chain 5 (EC 1.6.5.3).
[Mouse-ear cress] {Arabidopsis thaliana}, partial (18%)
Length = 788
Score = 24.3 bits (51), Expect = 8.4
Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Frame = -1
Query: 33 DQATNIILD--ESHERVFSTKEGVQLIALGLYIIR 65
D+ T++I + ES + S K ++LI G+YIIR
Sbjct: 647 DRRTSVIEEAIESITKSTSLKGSLELIRYGIYIIR 543
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.138 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,936,814
Number of Sequences: 36976
Number of extensions: 16756
Number of successful extensions: 63
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 63
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 63
length of query: 98
length of database: 9,014,727
effective HSP length: 74
effective length of query: 24
effective length of database: 6,278,503
effective search space: 150684072
effective search space used: 150684072
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0048.13