BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0043b.5
(298 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80190 similar to GP|21592409|gb|AAM64360.1 unknown {Arabidopsi... 417 e-117
TC88033 similar to GP|18086359|gb|AAL57642.1 AT5g45560/MFC19_23 ... 197 3e-51
TC89885 similar to GP|8978350|dbj|BAA98203.1 emb|CAB77600.1~gene... 190 6e-49
BG455125 similar to GP|21618178|gb unknown {Arabidopsis thaliana... 176 1e-44
BG582031 similar to GP|13384376|gb| unknown protein {Oryza sativ... 60 1e-09
TC92852 similar to GP|20453052|gb|AAM19771.1 At1g10410/F14N23_31... 37 0.009
TC89587 similar to PIR|C86306|C86306 prenyl transferase homolog ... 30 0.86
TC92802 homologue to PIR|T07371|T07371 dnaJ protein homolog - po... 29 1.9
BG646574 weakly similar to GP|6520167|dbj| ZW18 {Arabidopsis tha... 28 3.3
BQ157967 28 4.2
TC80451 28 4.2
TC80717 weakly similar to GP|6729021|gb|AAF27017.1| hypothetical... 28 5.5
>TC80190 similar to GP|21592409|gb|AAM64360.1 unknown {Arabidopsis
thaliana}, partial (79%)
Length = 1062
Score = 417 bits (1073), Expect = e-117
Identities = 214/285 (75%), Positives = 243/285 (85%), Gaps = 12/285 (4%)
Frame = +2
Query: 15 IMCPQTNTHRSSGE----TVDWISDAINGGSLRHVDLHTGTNGWASPPGDLFSLRSTNYF 70
+MC N RSSG T DW ++AINGGSLRHVDL+TGTNGWASPPGD+FSLRS +YF
Sbjct: 92 LMCTTGNNSRSSGSDSGSTTDWTTEAINGGSLRHVDLNTGTNGWASPPGDVFSLRSQSYF 271
Query: 71 TKRQKSPAGNYLLSPAGMDWLKSTAKLENVLARADNRVSNALR-------TRKSFIFAVN 123
TK+QKSPAG+YLLSP GMDWLKS+ KL+NVL R+DNRV+NAL+ + KSFIFAVN
Sbjct: 272 TKKQKSPAGDYLLSPIGMDWLKSSTKLDNVLNRSDNRVANALKKAQSNGKSLKSFIFAVN 451
Query: 124 LQIPGGKDHHSAVFYFATEEPIQPGSLLDRFVNGDDGFRNQRFKLVNRIVKGPWIVKKAV 183
LQIPG K+HHSAVFYFATE+P+Q GSLL RF++GDD FRNQRFKLVNRIVKGPWIVKKAV
Sbjct: 452 LQIPG-KEHHSAVFYFATEDPVQSGSLLGRFIDGDDAFRNQRFKLVNRIVKGPWIVKKAV 628
Query: 184 GNYSACLLGKALTCNYHRGSNYLEIDVDIGSSAIANAILHLALGYVTAVTIDMGFVIEAQ 243
GNYSACLLGKALTC+YHRG+NY E DVDIGSSAIANAIL LALGYVT+VTIDMGFV+EAQ
Sbjct: 629 GNYSACLLGKALTCHYHRGANYFEADVDIGSSAIANAILRLALGYVTSVTIDMGFVVEAQ 808
Query: 244 AEEELPERLIGAVRVCQMEMSSA-TVVDSLYAPPVARGSGLAKVN 287
EEELPE+LIGA+RVCQMEM SA T+VD AP + G+A+VN
Sbjct: 809 TEEELPEKLIGAIRVCQMEMGSACTIVD---APRI----GIAQVN 922
>TC88033 similar to GP|18086359|gb|AAL57642.1 AT5g45560/MFC19_23
{Arabidopsis thaliana}, partial (52%)
Length = 1608
Score = 197 bits (502), Expect = 3e-51
Identities = 98/227 (43%), Positives = 146/227 (64%)
Frame = +3
Query: 40 GSLRHVDLHTGTNGWASPPGDLFSLRSTNYFTKRQKSPAGNYLLSPAGMDWLKSTAKLEN 99
G+LR D + W G F +RS ++ + K+PAG +L+ +DW K + ++++
Sbjct: 627 GNLRRDDRDNARDCWKISDGSNFRVRSKHFCYDKSKAPAGKHLMDLVAVDWFKDSKRMDH 806
Query: 100 VLARADNRVSNALRTRKSFIFAVNLQIPGGKDHHSAVFYFATEEPIQPGSLLDRFVNGDD 159
V A+ + + F +N+Q+P H+S VFYF T+E + PG+LL RFV+GDD
Sbjct: 807 V-AKRPGCAAQVASEKGYFSIIINVQVPASS-HYSMVFYFVTKELV-PGTLLQRFVDGDD 977
Query: 160 GFRNQRFKLVNRIVKGPWIVKKAVGNYSACLLGKALTCNYHRGSNYLEIDVDIGSSAIAN 219
FRN R KL+ + KG WIV+++VG+ + CLLGKA+ CNY RG YLEIDVDIGSS +AN
Sbjct: 978 EFRNSRLKLIPSVPKGSWIVRQSVGS-TPCLLGKAVDCNYIRGPKYLEIDVDIGSSTVAN 1154
Query: 220 AILHLALGYVTAVTIDMGFVIEAQAEEELPERLIGAVRVCQMEMSSA 266
+L L +G +T + +DM F+++ +ELPERLIGAVR+ +E+ +A
Sbjct: 1155GVLGLVIGVITTLVVDMAFLVQGNTPDELPERLIGAVRISHLELKTA 1295
>TC89885 similar to GP|8978350|dbj|BAA98203.1
emb|CAB77600.1~gene_id:K3D20.2~similar to unknown
protein {Arabidopsis thaliana}, partial (40%)
Length = 1226
Score = 190 bits (482), Expect = 6e-49
Identities = 100/227 (44%), Positives = 140/227 (61%)
Frame = +1
Query: 40 GSLRHVDLHTGTNGWASPPGDLFSLRSTNYFTKRQKSPAGNYLLSPAGMDWLKSTAKLEN 99
GSLR TN W SP G F +R NY K G+ LL +DW ++
Sbjct: 424 GSLRKGMDDNDTNCWTSPSGKGFMIRGKNYLKDNSKVVGGDPLLKLIAVDWFTVNKSVDR 603
Query: 100 VLARADNRVSNALRTRKSFIFAVNLQIPGGKDHHSAVFYFATEEPIQPGSLLDRFVNGDD 159
+ V + + + FI VNLQ+P K ++S V Y+A + PI SLL +FV+G D
Sbjct: 604 IALHPRCLVQSEVGKKLPFILVVNLQVPA-KPNYSLVLYYAADRPINKKSLLAKFVDGSD 780
Query: 160 GFRNQRFKLVNRIVKGPWIVKKAVGNYSACLLGKALTCNYHRGSNYLEIDVDIGSSAIAN 219
FR+ RFKL+ IV+G W+VK+AVG ACLLGKA+TC Y R N+LEIDVDIGSS++A
Sbjct: 781 VFRDSRFKLIPSIVEGYWMVKRAVGT-KACLLGKAVTCKYFRQDNFLEIDVDIGSSSVAR 957
Query: 220 AILHLALGYVTAVTIDMGFVIEAQAEEELPERLIGAVRVCQMEMSSA 266
++ L LGYVT++ +D+ +IEA+ E ELPE ++G VR+ ++++ SA
Sbjct: 958 GVIGLVLGYVTSLVVDLAILIEAKEEAELPEYILGTVRLNRLKLESA 1098
>BG455125 similar to GP|21618178|gb unknown {Arabidopsis thaliana}, partial
(52%)
Length = 638
Score = 176 bits (445), Expect = 1e-44
Identities = 81/184 (44%), Positives = 117/184 (63%), Gaps = 5/184 (2%)
Frame = +2
Query: 31 DWISDAINGGSLRHVDLHTGTNGWASPPGDLFSLRSTNYFTKRQKSPAGNYLLSPAGMDW 90
+WI +GG++ +D +NGW SPPGD F +R YFT + K P +YLL P G DW
Sbjct: 89 EWIDRIRSGGAIPFLDPGKCSNGWGSPPGDAFMVRGPEYFTTKVKIPGSDYLLKPLGFDW 268
Query: 91 LKSTAKLENVLARADNRVSNALRTR-----KSFIFAVNLQIPGGKDHHSAVFYFATEEPI 145
+ S+ K+ +L ++RV + + F++A NLQ+P KD++SAV YF T+EPI
Sbjct: 269 ITSSTKIGEILKHPNSRVRKVIENEFPDGDRPFVWAFNLQLPT-KDNYSAVAYFTTKEPI 445
Query: 146 QPGSLLDRFVNGDDGFRNQRFKLVNRIVKGPWIVKKAVGNYSACLLGKALTCNYHRGSNY 205
GS++DRF+ GDD FR R K++ IV GPWIV+KAVG + C++G+AL C Y N+
Sbjct: 446 AEGSVMDRFLKGDDAFRTSRLKMIANIVNGPWIVRKAVGEQAICIIGRALACKYCVTENF 625
Query: 206 LEID 209
+E+D
Sbjct: 626 MEVD 637
>BG582031 similar to GP|13384376|gb| unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (7%)
Length = 697
Score = 60.1 bits (144), Expect = 1e-09
Identities = 30/73 (41%), Positives = 47/73 (64%), Gaps = 1/73 (1%)
Frame = +3
Query: 195 LTCNYHRGS-NYLEIDVDIGSSAIANAILHLALGYVTAVTIDMGFVIEAQAEEELPERLI 253
L+CN + G ++I VD+GSS IA +++L LGY+ + I+M F+I+ EELPE L+
Sbjct: 126 LSCNAYIGIVASVQIGVDVGSSTIARGVVNLVLGYLNHLVIEMAFLIQGNTREELPEVLL 305
Query: 254 GAVRVCQMEMSSA 266
G R+ ++ S A
Sbjct: 306 GTCRLNHLDASKA 344
>TC92852 similar to GP|20453052|gb|AAM19771.1 At1g10410/F14N23_31
{Arabidopsis thaliana}, partial (27%)
Length = 526
Score = 37.0 bits (84), Expect = 0.009
Identities = 18/61 (29%), Positives = 33/61 (53%), Gaps = 1/61 (1%)
Frame = +3
Query: 199 YHRGSNYLEIDVDIGSSA-IANAILHLALGYVTAVTIDMGFVIEAQAEEELPERLIGAVR 257
++ G NY EID+D+ I+ + + T+D+G I+ EELPE+++ +R
Sbjct: 189 FYSGENYFEIDIDMHRFGYISRKGFEAFMDRLKICTLDIGLTIQGNKVEELPEQVLCCIR 368
Query: 258 V 258
+
Sbjct: 369 L 371
>TC89587 similar to PIR|C86306|C86306 prenyl transferase homolog [imported]
- Arabidopsis thaliana, partial (27%)
Length = 714
Score = 30.4 bits (67), Expect = 0.86
Identities = 19/63 (30%), Positives = 29/63 (45%), Gaps = 5/63 (7%)
Frame = +3
Query: 14 LIMCPQTNTHRSSGETVDWISDAINGGSLRHVDLHTGTNGWASP-----PGDLFSLRSTN 68
L+ C + NTH+ ++D +NGG+ DL G N + P P LF + S +
Sbjct: 258 LVCCKKRNTHQYRVSSMDTAEPTLNGGTQAIKDLAKGLNLKSYPATPISPNRLFEVVSDD 437
Query: 69 YFT 71
T
Sbjct: 438 LLT 446
>TC92802 homologue to PIR|T07371|T07371 dnaJ protein homolog - potato,
partial (51%)
Length = 707
Score = 29.3 bits (64), Expect = 1.9
Identities = 20/70 (28%), Positives = 34/70 (48%), Gaps = 6/70 (8%)
Frame = +1
Query: 223 HLALGYVTAVTIDMGFVIEAQAEEELPE-RLIGAV-----RVCQMEMSSATVVDSLYAPP 276
HL +++ V + V+EA EE+ E R++ + R+C ME + ++Y
Sbjct: 331 HLTFSHLSLVAAHLVEVVEAAEEEDKEEERMLSTLSKFLWRICTMEHQRSCHYLAMYCAR 510
Query: 277 VARGSGLAKV 286
ARG L +V
Sbjct: 511 SARGKDLNQV 540
>BG646574 weakly similar to GP|6520167|dbj| ZW18 {Arabidopsis thaliana},
partial (10%)
Length = 754
Score = 28.5 bits (62), Expect = 3.3
Identities = 16/52 (30%), Positives = 25/52 (47%)
Frame = +3
Query: 123 NLQIPGGKDHHSAVFYFATEEPIQPGSLLDRFVNGDDGFRNQRFKLVNRIVK 174
+LQ+P ++ + + TEE +QP S D +N NQ L N +K
Sbjct: 348 SLQLPDREEDATHNINYLTEESVQPFSWDDHLLNSFQSLANQLLYLWNVFLK 503
>BQ157967
Length = 285
Score = 28.1 bits (61), Expect = 4.2
Identities = 23/84 (27%), Positives = 38/84 (44%)
Frame = +3
Query: 142 EEPIQPGSLLDRFVNGDDGFRNQRFKLVNRIVKGPWIVKKAVGNYSACLLGKALTCNYHR 201
+ P+ + +D G R+ ++ K ++K VGN+ +C G+ LT R
Sbjct: 12 QNPLNKSNRIDPQYEGVSSSRSN----LSSATKSDLLIK*NVGNFLSC*WGRILT----R 167
Query: 202 GSNYLEIDVDIGSSAIANAILHLA 225
S +G S +ANA+ HLA
Sbjct: 168 SS*-------MGRSGLANAVKHLA 218
>TC80451
Length = 1100
Score = 28.1 bits (61), Expect = 4.2
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = -1
Query: 45 VDLHTGTNGWASPPGDLFSLRSTNYFTKRQKSPAGNYLLSPAGMDWLKS 93
VDL T G + L + S +K + SPA + LS G DWL S
Sbjct: 461 VDLELCTGGNSMRGSVL*LIFSVQLGSKAENSPADIFELSSCGFDWLVS 315
>TC80717 weakly similar to GP|6729021|gb|AAF27017.1| hypothetical protein
{Arabidopsis thaliana}, partial (28%)
Length = 1198
Score = 27.7 bits (60), Expect = 5.5
Identities = 26/91 (28%), Positives = 39/91 (42%), Gaps = 15/91 (16%)
Frame = +1
Query: 25 SSGETVDWISDAINGGSLRHVDLHTGTNGWASPPGDL--FSLRSTNYFT---KRQKSPAG 79
S G V ++S SL + G + PP +L FS R ++ +R+ P+G
Sbjct: 757 SDGVKVSYLSKVKGFVSLFQQTVQNGISLCCIPPTELYRFSHRVPSFVMTGKEREGVPSG 936
Query: 80 NYLLSPAGMDW----------LKSTAKLENV 100
+Y L PAG+ +K KL NV
Sbjct: 937 SYELDPAGLPMSVFQVGDHGDMKDNEKLNNV 1029
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,752,255
Number of Sequences: 36976
Number of extensions: 112349
Number of successful extensions: 451
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 440
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 442
length of query: 298
length of database: 9,014,727
effective HSP length: 96
effective length of query: 202
effective length of database: 5,465,031
effective search space: 1103936262
effective search space used: 1103936262
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0043b.5