BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0036b.1
(122 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91208 homologue to SP|Q9BBP1|NU6C_LOTJA NADH-plastoquinone oxi... 29 0.39
TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectineste... 28 1.1
TC87657 similar to GP|13430796|gb|AAK26020.1 unknown protein {Ar... 28 1.1
TC84086 weakly similar to GP|16519223|gb|AAL25128.1 cellulose sy... 27 1.5
TC81304 auxin efflux carrier protein [Medicago truncatula] 27 1.9
BQ135437 27 1.9
AL389714 26 4.3
TC82740 weakly similar to PIR|F86420|F86420 hypothetical protein... 26 4.3
BG580775 weakly similar to GP|15529149|gb AT3g21550/MIL23_11 {Ar... 25 5.6
TC91953 weakly similar to GP|20268729|gb|AAM14068.1 unknown prot... 25 5.6
TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protei... 25 5.6
BG646771 similar to GP|11994562|db emb|CAB68186.1~gene_id:K20M4.... 25 5.6
TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chape... 25 5.6
TC80617 weakly similar to PIR|F96672|F96672 Similar to Flavonol ... 25 5.6
TC90040 homologue to GP|9294658|dbj|BAB03007.1 gene_id:F14O13.8~... 25 7.3
TC82374 similar to GP|21689765|gb|AAM67526.1 putative arsA-like ... 25 7.3
TC85793 similar to PIR|T31428|T31428 fiber annexin - upland cott... 25 7.3
TC78170 similar to GP|17979531|gb|AAL50100.1 At1g76150/T23E18_38... 25 7.3
CA859660 similar to GP|10241917|dbj 34 kDa protein {Hop latent v... 25 7.3
BF646753 similar to GP|21751020|dbj unnamed protein product {Hom... 25 9.6
>TC91208 homologue to SP|Q9BBP1|NU6C_LOTJA NADH-plastoquinone oxidoreductase
chain 6 chloroplast (EC 1.6.5.3). {Lotus japonicus},
complete
Length = 1081
Score = 29.3 bits (64), Expect = 0.39
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +1
Query: 46 GIFYKGLNIVLCILILQKDKLIRTHTCTLLHQLMGKSKVFDANCY 90
GIF+ LN++ C + KLIR+ + L H L+ + +F Y
Sbjct: 748 GIFFSQLNLLNCCSY*NESKLIRSWSMMLEHVLVLSAYLFSIGIY 882
>TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectinesterase
{Arabidopsis thaliana}, partial (34%)
Length = 691
Score = 27.7 bits (60), Expect = 1.1
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = -3
Query: 88 NCYSYPPPLFRSLKIGELSKMPLFKK 113
NC+ +P P+FR +G L ++ +F K
Sbjct: 164 NCFVFPTPVFRDNNVGFLLQITVFLK 87
>TC87657 similar to GP|13430796|gb|AAK26020.1 unknown protein {Arabidopsis
thaliana}, partial (78%)
Length = 1738
Score = 27.7 bits (60), Expect = 1.1
Identities = 17/46 (36%), Positives = 22/46 (46%)
Frame = -3
Query: 29 TCNFSELLEILSRHVVAGIFYKGLNIVLCILILQKDKLIRTHTCTL 74
TC+F ++E LSR+V+ F ILQ DKL H L
Sbjct: 1541 TCSFLLIVEYLSRYVIRAFF----------CILQVDKLRNKHMLAL 1434
>TC84086 weakly similar to GP|16519223|gb|AAL25128.1 cellulose
synthase-like protein OsCslA9 {Oryza sativa}, partial
(23%)
Length = 489
Score = 27.3 bits (59), Expect = 1.5
Identities = 15/49 (30%), Positives = 19/49 (38%)
Frame = +1
Query: 42 HVVAGIFYKGLNIVLCILILQKDKLIRTHTCTLLHQLMGKSKVFDANCY 90
H +F + L + KD RTH C LLH + NCY
Sbjct: 13 HKQESVFVEEATYCLQFFLYSKDCGARTHICVLLH-------IVTCNCY 138
>TC81304 auxin efflux carrier protein [Medicago truncatula]
Length = 831
Score = 26.9 bits (58), Expect = 1.9
Identities = 19/55 (34%), Positives = 27/55 (48%)
Frame = +1
Query: 64 DKLIRTHTCTLLHQLMGKSKVFDANCYSYPPPLFRSLKIGELSKMPLFKKKKFSL 118
D L+ + LLH L+ D+ +S+ PLFR L ELS + L K + L
Sbjct: 220 DSLLVQLSWQLLHLLL------DSKVFSFMLPLFRQLFHKELSHLSLLKNIMYIL 366
>BQ135437
Length = 854
Score = 26.9 bits (58), Expect = 1.9
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = +1
Query: 84 VFDANCYSYPPPLFRSLKIGELSKMPLFKKKKFSLSPLT 122
++ A Y PPP+FR+L + + LF K + LT
Sbjct: 595 IYSAIMYMIPPPMFRALALIFSQSLCLFLKPHIIIDILT 711
>AL389714
Length = 525
Score = 25.8 bits (55), Expect = 4.3
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = -2
Query: 26 HSSTCNFSELLEILSRHVVAGIFYK 50
H CNF +LEI + H++ +F +
Sbjct: 329 HFLRCNFVIILEIHTNHIIESVFMR 255
>TC82740 weakly similar to PIR|F86420|F86420 hypothetical protein AAG12910.1
[imported] - Arabidopsis thaliana, partial (5%)
Length = 615
Score = 25.8 bits (55), Expect = 4.3
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 5/38 (13%)
Frame = -2
Query: 53 NIVLCILILQKDKLIRTHTCTLLH-----QLMGKSKVF 85
N+VLC LIL K+K + H + L+GKS F
Sbjct: 242 NMVLCCLILPKEKEV*LHLFVISSSCSGVNLIGKSSTF 129
>BG580775 weakly similar to GP|15529149|gb AT3g21550/MIL23_11 {Arabidopsis
thaliana}, partial (87%)
Length = 557
Score = 25.4 bits (54), Expect = 5.6
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +2
Query: 34 ELLEILSRHVVAGIFYKGLNIVLCILILQKDKLIRTHTCTLLHQLMG 80
E L +LS + + L ++LCI + +R +C H+L+G
Sbjct: 344 ETLSMLSCR*LCSRCWDCLTLMLCIAFTRNLSRLRRFSCRCYHRLLG 484
>TC91953 weakly similar to GP|20268729|gb|AAM14068.1 unknown protein
{Arabidopsis thaliana}, partial (33%)
Length = 972
Score = 25.4 bits (54), Expect = 5.6
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = +2
Query: 75 LHQLMGKSKVFDANCYSYPPPLFRSLK 101
L QL K D+ C +PP +FR L+
Sbjct: 62 LGQLKSMRKTADSACVVFPPKIFRLLE 142
>TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protein 4 precursor
(BiP 4) (78 kDa glucose-regulated protein homolog 4) (GRP
78-4)., partial (94%)
Length = 2265
Score = 25.4 bits (54), Expect = 5.6
Identities = 8/31 (25%), Positives = 17/31 (54%)
Frame = +2
Query: 1 MSDELDWYEPNDSLRSDRFIVSLKDHSSTCN 31
+ + L+W + N ++ + F LK+ + CN
Sbjct: 1868 VKEALEWLDDNQTVEKEEFEEKLKEVEAVCN 1960
>BG646771 similar to GP|11994562|db emb|CAB68186.1~gene_id:K20M4.4~strong
similarity to unknown protein {Arabidopsis thaliana},
partial (23%)
Length = 663
Score = 25.4 bits (54), Expect = 5.6
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +3
Query: 4 ELDWYEPNDSLRSDRFIVSLKDHSSTCNFSELL 36
++D + D LRS R V L++HSS ++SE +
Sbjct: 441 KMDPVDEADELRSLRSSVHLRNHSSEEDYSETI 539
>TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chaperone BiP-A
- soybean, partial (52%)
Length = 1231
Score = 25.4 bits (54), Expect = 5.6
Identities = 8/31 (25%), Positives = 17/31 (54%)
Frame = +1
Query: 1 MSDELDWYEPNDSLRSDRFIVSLKDHSSTCN 31
+ + L+W + N S+ + + LK+ + CN
Sbjct: 859 VKEALEWLDDNQSVEKEEYEEKLKEVEAVCN 951
>TC80617 weakly similar to PIR|F96672|F96672 Similar to Flavonol
3-O-Glucosyltransferase [imported] - Arabidopsis
thaliana, partial (32%)
Length = 941
Score = 25.4 bits (54), Expect = 5.6
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +2
Query: 22 SLKDHSSTCNFSELLEIL 39
SLK HS++CN S ++IL
Sbjct: 122 SLKTHSTSCNISIFIQIL 175
>TC90040 homologue to GP|9294658|dbj|BAB03007.1 gene_id:F14O13.8~unknown
protein {Arabidopsis thaliana}, partial (2%)
Length = 1571
Score = 25.0 bits (53), Expect = 7.3
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 54 IVLCILILQKDKLIRTHTCTLLH 76
I LC+LIL+ I H C +LH
Sbjct: 1061 IELCVLILRNQYPIGIHVC*VLH 993
>TC82374 similar to GP|21689765|gb|AAM67526.1 putative arsA-like protein
hASNA-I {Arabidopsis thaliana}, partial (97%)
Length = 1176
Score = 25.0 bits (53), Expect = 7.3
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +3
Query: 6 DWYEPNDSLRSDRFIVSLKDHSSTCNFS 33
DW++ + SLRS I SL SST S
Sbjct: 684 DWFKNSQSLRSTHTISSLIKLSSTMKMS 767
>TC85793 similar to PIR|T31428|T31428 fiber annexin - upland cotton,
complete
Length = 1272
Score = 25.0 bits (53), Expect = 7.3
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = -1
Query: 34 ELLEILSRHVVAGIFYKGLNIVLCILILQKDKLIRTHTCTLLHQ 77
EL E+ S VV+ IF+K + I +L K +LIR + + HQ
Sbjct: 480 ELTEV-SGCVVSNIFFKRFLMKSMISLLCKKQLIRRSSAS*FHQ 352
>TC78170 similar to GP|17979531|gb|AAL50100.1 At1g76150/T23E18_38
{Arabidopsis thaliana}, partial (90%)
Length = 955
Score = 25.0 bits (53), Expect = 7.3
Identities = 29/113 (25%), Positives = 45/113 (39%), Gaps = 6/113 (5%)
Frame = +1
Query: 8 YEPNDSLRSDRFIVSLKDHSSTCNFSELLEILSRHVVAGIFYKGLNIVLCILILQKDK-- 65
Y+P L ++I K S+C+ I ++ +AG+ KG +L I +K
Sbjct: 289 YDPRLLLHGQQYIELCKPFPSSCH------IQNKVSLAGLHDKGKAAILEIETKSYEKES 450
Query: 66 ---LIRTHTCTLLHQLMGKSKVFDANCYS-YPPPLFRSLKIGELSKMPLFKKK 114
L T L G SK YS YP ++KI E +F+ +
Sbjct: 451 GDLLCMNRTTVYLRGAGGFSKTSKPFSYSNYPSNQTSAVKIPESKPFAVFEDR 609
>CA859660 similar to GP|10241917|dbj 34 kDa protein {Hop latent virus},
partial (5%)
Length = 179
Score = 25.0 bits (53), Expect = 7.3
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = -1
Query: 32 FSELLEI-LSRHVVAGIFYKGLNIVLCILILQKDKLIRTHTCTLLH 76
F +LEI L H+V G +Y+GL CI ++ + L+ LLH
Sbjct: 152 FLMILEIYLLSHLVYGNYYQGLQKHQCIFLVIRLYLLTR*FFLLLH 15
>BF646753 similar to GP|21751020|dbj unnamed protein product {Homo sapiens},
partial (6%)
Length = 657
Score = 24.6 bits (52), Expect = 9.6
Identities = 17/67 (25%), Positives = 33/67 (48%), Gaps = 2/67 (2%)
Frame = +3
Query: 46 GIFYKGLNIVLCILILQKDKLIRTHTCTLLHQLMGK--SKVFDANCYSYPPPLFRSLKIG 103
G+ + ++I +C+L+LQ + + LL + +GK + +N PP L +
Sbjct: 240 GLLLQQISITICLLLLQHNLCLLLLLHVLLSRELGKMIDLLVISNSSVVPPLLCQQQVST 419
Query: 104 ELSKMPL 110
+L+ PL
Sbjct: 420 KLNLFPL 440
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.140 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,723,681
Number of Sequences: 36976
Number of extensions: 69472
Number of successful extensions: 503
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 502
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 503
length of query: 122
length of database: 9,014,727
effective HSP length: 84
effective length of query: 38
effective length of database: 5,908,743
effective search space: 224532234
effective search space used: 224532234
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0036b.1