BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0035.10
(150 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB892176 PIR|T09286|T09 malate dehydrogenase (EC 1.1.1.37) precu... 29 0.82
TC88857 weakly similar to GP|10177408|dbj|BAB10539. gene_id:K24M... 28 1.8
TC80583 squalene monooxygenase 1 [Medicago truncatula] 27 2.4
TC80056 similar to GP|12322859|gb|AAG51421.1 unknown protein co... 27 2.4
TC77298 similar to PIR|T05726|T05726 GH1 protein - soybean (frag... 27 3.1
TC78874 similar to PIR|T49098|T49098 hypothetical protein F4F15.... 27 3.1
TC85489 similar to GP|16649103|gb|AAL24403.1 Unknown protein {Ar... 27 4.1
AW584499 similar to GP|21536514|gb 40S ribosomal protein S2 homo... 27 4.1
CA919708 similar to GP|23318573|gb| Sequence 89 from patent US 6... 26 5.3
BE941075 26 5.3
TC89885 similar to GP|8978350|dbj|BAA98203.1 emb|CAB77600.1~gene... 26 5.3
TC86128 weakly similar to SP|P14298|CFI_PHAVU Chalcone--flavonon... 26 5.3
TC83477 similar to GP|22831055|dbj|BAC15918. receptor kinase-lik... 26 7.0
AL370397 weakly similar to SP|Q43056|CFI_ Chalcone--flavonone is... 26 7.0
TC77430 similar to GP|13676413|dbj|BAB41197. hypothetical protei... 26 7.0
TC90640 similar to GP|23334466|gb|AAN27897.1 Sequence 6 from pat... 25 9.1
TC77364 similar to GP|20160852|dbj|BAB89791. contains EST AU0557... 25 9.1
TC88229 similar to PIR|E84471|E84471 probable beta-1 3-glucanase... 25 9.1
TC83468 similar to PIR|G71443|G71443 hypothetical protein - Arab... 25 9.1
TC80825 similar to PIR|F84614|F84614 probable kinesin heavy chai... 25 9.1
>CB892176 PIR|T09286|T09 malate dehydrogenase (EC 1.1.1.37) precursor -
alfalfa, partial (41%)
Length = 615
Score = 28.9 bits (63), Expect = 0.82
Identities = 17/58 (29%), Positives = 29/58 (49%), Gaps = 4/58 (6%)
Frame = +3
Query: 19 CCSYITLHMQ----RKLKKKKKNTTQHNDRDSSELRDQVSIHPSSSSFIITMLLGPSL 72
CC+ I Q +K KKKKKN+ + +R+ R++ SF+ ++ PS+
Sbjct: 45 CCNCILFTRQFCYVKKKKKKKKNSRRERERERERERER---ERERESFLKASMMRPSI 209
>TC88857 weakly similar to GP|10177408|dbj|BAB10539.
gene_id:K24M7.17~unknown protein {Arabidopsis thaliana},
partial (47%)
Length = 793
Score = 27.7 bits (60), Expect = 1.8
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 15/82 (18%)
Frame = +3
Query: 12 KSGNHRPCC-SY------------ITLHMQRKLKK--KKKNTTQHNDRDSSELRDQVSIH 56
K +H+ CC SY +TL Q+K KK KKN T H+ + ++++
Sbjct: 36 KYPDHKICCLSYTQKCLVELDQLAVTLAYQKKNKKLNTKKNKTNHSTNQQAHHKERLVSS 215
Query: 57 PSSSSFIITMLLGPSLHQCGWM 78
S +S + +LL S W+
Sbjct: 216 HSDNSIV*LLLL--SYQPVAWL 275
>TC80583 squalene monooxygenase 1 [Medicago truncatula]
Length = 1830
Score = 27.3 bits (59), Expect = 2.4
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +1
Query: 24 TLHMQRKLKKKKKNTTQHNDRDSSELR 50
TLH ++ ++++NTTQHN SS LR
Sbjct: 13 TLHPAKQ--RERENTTQHNTAPSSSLR 87
>TC80056 similar to GP|12322859|gb|AAG51421.1 unknown protein contains
TNFR/NGFR cysteine-rich region; 28705-27186 {Arabidopsis
thaliana}, partial (98%)
Length = 879
Score = 27.3 bits (59), Expect = 2.4
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +3
Query: 73 HQCGWMDGCMPTRNS 87
H CGWM+ C+ RN+
Sbjct: 588 HHCGWMNNCIGERNT 632
>TC77298 similar to PIR|T05726|T05726 GH1 protein - soybean (fragment),
partial (91%)
Length = 1571
Score = 26.9 bits (58), Expect = 3.1
Identities = 9/24 (37%), Positives = 14/24 (57%)
Frame = +1
Query: 57 PSSSSFIITMLLGPSLHQCGWMDG 80
P + S I+ +LGP C W++G
Sbjct: 247 PLNGSICISTILGPFFSNCSWVEG 318
>TC78874 similar to PIR|T49098|T49098 hypothetical protein F4F15.300 -
Arabidopsis thaliana, partial (74%)
Length = 1630
Score = 26.9 bits (58), Expect = 3.1
Identities = 30/102 (29%), Positives = 42/102 (40%)
Frame = -2
Query: 40 QHNDRDSSELRDQVSIHPSSSSFIITMLLGPSLHQCGWMDGCMPTRNSYDTSGSLSHADT 99
+HN SSELRD+V SS+F I L PS+ + RNS S+S D
Sbjct: 795 RHNSIFSSELRDRVVTGTPSSTFQI--LAEPSVE--------VEARNSESRLKSISRTDL 646
Query: 100 RATSAKATCLLSWFLLHQSLSRSLTYPQCKVQTPKKYQVVAV 141
A + + L H + R + P P +V+ V
Sbjct: 645 CALGSSK---IMRMLGHSIILRCPSDPPAANLDPSTLKVIHV 529
>TC85489 similar to GP|16649103|gb|AAL24403.1 Unknown protein {Arabidopsis
thaliana}, partial (26%)
Length = 858
Score = 26.6 bits (57), Expect = 4.1
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 82 MPTRNSYDTSGSLSHADTRATSAKAT 107
M T +SY+TS + H DT S+ AT
Sbjct: 380 MKTSSSYNTSSASEHQDTTIISSAAT 457
>AW584499 similar to GP|21536514|gb 40S ribosomal protein S2 homolog
{Arabidopsis thaliana}, partial (40%)
Length = 664
Score = 26.6 bits (57), Expect = 4.1
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 82 MPTRNSYDTSGSLSHADTRATSAKAT 107
M T +SY+TS + H DT S+ AT
Sbjct: 95 MKTSSSYNTSSASEHQDTTIISSAAT 172
>CA919708 similar to GP|23318573|gb| Sequence 89 from patent US 6417428,
partial (35%)
Length = 751
Score = 26.2 bits (56), Expect = 5.3
Identities = 18/72 (25%), Positives = 33/72 (45%), Gaps = 6/72 (8%)
Frame = +3
Query: 81 CMPTRNSYDTSGSLSHADTRATSAKATCLLSWFLLHQSLSRSLTYPQCKVQT----PKKY 136
C + N + +G S +++ +CLL+ F +R + CK++T P Y
Sbjct: 528 CDNSYNGVNVTGH*SIVWSKSFGGFCSCLLNIFYRRSCWNRKAPHRVCKIETGIAAPSCY 707
Query: 137 Q--VVAVGLEMG 146
+ +V GL +G
Sbjct: 708 RGNIVGSGLHLG 743
>BE941075
Length = 651
Score = 26.2 bits (56), Expect = 5.3
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = +3
Query: 1 MWRLVPSLTRMKSGNHRPC 19
+WR+ PS +RM+S ++PC
Sbjct: 189 IWRIKPS*SRMRS*ENKPC 245
>TC89885 similar to GP|8978350|dbj|BAA98203.1
emb|CAB77600.1~gene_id:K3D20.2~similar to unknown
protein {Arabidopsis thaliana}, partial (40%)
Length = 1226
Score = 26.2 bits (56), Expect = 5.3
Identities = 12/44 (27%), Positives = 26/44 (58%), Gaps = 3/44 (6%)
Frame = +1
Query: 110 LSWFLLHQSLSRSLTYPQCKVQT---PKKYQVVAVGLEMGSAPS 150
+ WF +++S+ R +P+C VQ+ K ++ V L++ + P+
Sbjct: 568 VDWFTVNKSVDRIALHPRCLVQSEVGKKLPFILVVNLQVPAKPN 699
>TC86128 weakly similar to SP|P14298|CFI_PHAVU Chalcone--flavonone isomerase
(EC 5.5.1.6) (Chalcone isomerase). [Kidney bean French
bean], partial (93%)
Length = 1088
Score = 26.2 bits (56), Expect = 5.3
Identities = 11/26 (42%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Frame = -1
Query: 109 LLSW-FLLHQSLSRSLTYPQCKVQTP 133
+L W FLLH+ + ++Y QC TP
Sbjct: 557 ILEWLFLLHRHMFLQISYGQCNFLTP 480
>TC83477 similar to GP|22831055|dbj|BAC15918. receptor kinase-like protein
{Oryza sativa (japonica cultivar-group)}, partial (4%)
Length = 919
Score = 25.8 bits (55), Expect = 7.0
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 3/34 (8%)
Frame = +1
Query: 61 SFIITMLLGP---SLHQCGWMDGCMPTRNSYDTS 91
SF + +L P LHQC M PT++S+ T+
Sbjct: 121 SFFLELLYFP*QKHLHQCSTMLPAQPTKHSHQTA 222
>AL370397 weakly similar to SP|Q43056|CFI_ Chalcone--flavonone isomerase (EC
5.5.1.6) (Chalcone isomerase). [Kudzu vine] {Pueraria
lobata}, partial (64%)
Length = 460
Score = 25.8 bits (55), Expect = 7.0
Identities = 11/26 (42%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Frame = -1
Query: 109 LLSW-FLLHQSLSRSLTYPQCKVQTP 133
+L W FLLH+ + ++Y QC TP
Sbjct: 442 VLEWLFLLHRHMFLQISYGQCNFLTP 365
>TC77430 similar to GP|13676413|dbj|BAB41197. hypothetical protein {Glycine
max}, partial (71%)
Length = 2529
Score = 25.8 bits (55), Expect = 7.0
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +2
Query: 2 WRLVPSLTRMKSGNHRPCCSYITLHMQRKLKKKKKN 37
WRL +TRM S H P I +H L + K+
Sbjct: 1760 WRLSLFITRMISLTHSPATRSIMIHKMEGLDIRNKS 1867
>TC90640 similar to GP|23334466|gb|AAN27897.1 Sequence 6 from patent US
6441271, partial (66%)
Length = 737
Score = 25.4 bits (54), Expect = 9.1
Identities = 12/43 (27%), Positives = 18/43 (40%)
Frame = -3
Query: 16 HRPCCSYITLHMQRKLKKKKKNTTQHNDRDSSELRDQVSIHPS 58
HR C LH+ R + K+ + +R+ Q HPS
Sbjct: 492 HRYVCHL*PLHVHRTVHVKQVDQAYDQEREFQNCYQQPQFHPS 364
>TC77364 similar to GP|20160852|dbj|BAB89791. contains EST
AU055715(S20012)~similar to nuclear
ribonucleoprotein~unknown protein, partial (50%)
Length = 2480
Score = 25.4 bits (54), Expect = 9.1
Identities = 15/51 (29%), Positives = 24/51 (46%), Gaps = 12/51 (23%)
Frame = -1
Query: 15 NHRPCCSYIT-------LHMQRKLKKKKKNTTQ-----HNDRDSSELRDQV 53
NH+PC S +T + M+RK ++K+ T HN D + D +
Sbjct: 2303 NHKPCLSNLT*KVLIGAMEMRRKTIHREKSRTSFYKLIHNQ*DHKKTEDGI 2151
>TC88229 similar to PIR|E84471|E84471 probable beta-1 3-glucanase [imported]
- Arabidopsis thaliana, partial (69%)
Length = 1368
Score = 25.4 bits (54), Expect = 9.1
Identities = 13/42 (30%), Positives = 23/42 (53%), Gaps = 5/42 (11%)
Frame = +3
Query: 8 LTRMKSGN---HRPCCSYITLHMQRKLKKK--KKNTTQHNDR 44
+TR + G HRP CS I + ++ + +K K+ T H+ +
Sbjct: 174 ITRNRPGQTLRHRPGCSQILIRIRNQSNRKPPKRTTLSHSQK 299
>TC83468 similar to PIR|G71443|G71443 hypothetical protein - Arabidopsis
thaliana, partial (51%)
Length = 1237
Score = 25.4 bits (54), Expect = 9.1
Identities = 15/35 (42%), Positives = 19/35 (53%), Gaps = 1/35 (2%)
Frame = -2
Query: 50 RDQVS-IHPSSSSFIITMLLGPSLHQCGWMDGCMP 83
RDQVS IHPS S +I ++H+ G C P
Sbjct: 924 RDQVS*IHPSVS*HMIFSFSASAVHRVGTNPTCHP 820
>TC80825 similar to PIR|F84614|F84614 probable kinesin heavy chain
[imported] - Arabidopsis thaliana, partial (16%)
Length = 1771
Score = 25.4 bits (54), Expect = 9.1
Identities = 21/56 (37%), Positives = 28/56 (49%), Gaps = 3/56 (5%)
Frame = -3
Query: 82 MPTRNSYDTSGSLSH---ADTRATSAKATCLLSWFLLHQSLSRSLTYPQCKVQTPK 134
+P RN T GS S + +R + CLL LH SL +SLT C++ PK
Sbjct: 491 IPIRN-VTTFGS*SGYHISKSRKRTIYILCLLKSLSLHISLCKSLT--ACQIHKPK 333
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.128 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,891,233
Number of Sequences: 36976
Number of extensions: 88680
Number of successful extensions: 688
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 685
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 687
length of query: 150
length of database: 9,014,727
effective HSP length: 87
effective length of query: 63
effective length of database: 5,797,815
effective search space: 365262345
effective search space used: 365262345
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0035.10