BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0031.12
(227 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I... 37 0.008
BQ141542 similar to GP|7295500|gb|A CG10918 gene product {Drosop... 35 0.018
BQ144197 homologue to GP|1841930|emb| ROX protein {Mus musculus}... 34 0.040
TC87784 weakly similar to PIR|D86292|D86292 protein F7H2.14 [imp... 33 0.068
CA991348 similar to PIR|D84685|D84 hypothetical protein At2g2848... 33 0.089
TC82135 33 0.089
TC76698 similar to PIR|S50766|S50766 dehydrin-related protein - ... 31 0.34
TC81657 weakly similar to PIR|T51957|T51957 metalloproteinase (E... 31 0.44
TC79994 weakly similar to GP|14423560|gb|AAK62462.1 Unknown prot... 30 0.58
TC76342 similar to PIR|A86444|A86444 probable RNA helicase [impo... 30 0.58
TC78017 similar to GP|16648718|gb|AAL25551.1 AT5g64130/MHJ24_11 ... 30 0.76
TC79345 similar to GP|21537136|gb|AAM61477.1 putative phosphatid... 30 0.99
TC92460 similar to GP|22202705|dbj|BAC07363. hypothetical protei... 30 0.99
BI271286 similar to PIR|T48519|T485 hypothetical protein F15N18.... 29 1.3
TC76866 similar to GP|2909420|emb|CAA12026.1 LEA protein {Cicer ... 29 1.7
TC82482 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3... 29 1.7
TC92202 similar to GP|14596161|gb|AAK68808.1 Unknown protein {Ar... 28 2.2
TC89852 similar to GP|15620063|gb|AAL03490.1 unknown {Rickettsia... 28 2.2
AJ499434 similar to PIR|B86197|B861 hypothetical protein [import... 28 2.2
CB893400 similar to PIR|B86197|B861 hypothetical protein [import... 28 2.2
>TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I3.230 -
Arabidopsis thaliana, partial (14%)
Length = 1074
Score = 36.6 bits (83), Expect = 0.008
Identities = 24/116 (20%), Positives = 52/116 (44%)
Frame = +3
Query: 30 PFQNTDLEKTEDQASNACKGNNNHKIQRNEQKYTRNSKINKITENAAPIITETTKKREEN 89
P + + + + + + K ++ ++ + K + K K EN A E ++E+
Sbjct: 291 PAKKSKVAEVDGEEKVKTKKVDDAAVEVEDDKKEKKKKKKKDKENGAAASDEEKVEKEK- 467
Query: 90 HKTLPERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKKQKINKKSK 145
++KH+ E + K K K+K + ++ +K KKK+K +K++K
Sbjct: 468 -----KKKHKEKGEDGSPEVEKSDKKKKKHKETSEVGSPEVDKSEKKKKKKDKEAK 620
>BQ141542 similar to GP|7295500|gb|A CG10918 gene product {Drosophila
melanogaster}, partial (10%)
Length = 1259
Score = 35.4 bits (80), Expect = 0.018
Identities = 34/127 (26%), Positives = 55/127 (42%), Gaps = 15/127 (11%)
Frame = +2
Query: 50 NNNHKIQR-NEQKYTRNSKINKITENAAPIITETTKKREENHKTL------PERK----- 97
N HK Q+ +EQ+ T NK E TKK+++ P +K
Sbjct: 410 NKTHKDQKGSEQQSTPTPTTNKQPYRTKEAKREATKKKKKGSSPTKVKTPHPSKK**EDS 589
Query: 98 --HRASNESKAKTQSKLKKMKRKTQNAG-QIMKQTSEKIVKKKQKINKKSKTIFTTSTER 154
H+ + + +T+S + K KT+ G +I +K+ KKQK K+ + ER
Sbjct: 590 TSHKRILKMEEETESTPRPGK*KTRKRGSRIQ*HPQKKLSNKKQKRKKRRRKANRAI*ER 769
Query: 155 AKQRNRR 161
++ NRR
Sbjct: 770 DRETNRR 790
>BQ144197 homologue to GP|1841930|emb| ROX protein {Mus musculus}, partial
(4%)
Length = 1170
Score = 34.3 bits (77), Expect = 0.040
Identities = 33/161 (20%), Positives = 70/161 (42%), Gaps = 8/161 (4%)
Frame = +2
Query: 36 LEKTEDQASNACKGNNNHK--IQRNEQKYTRNSKINKITENAAPIIT---ETTKKREENH 90
L++ Q+++ G+N + + QK+ R K +AA ETT R
Sbjct: 629 LQRNNKQSTHGKGGSNRGRKGSVKARQKHCRTPPTQKKGRDAARSTRSDRETTAHR**RE 808
Query: 91 KTLPERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKKQKINKKSKTIFTT 150
++ ++HRA +A+ K ++ + + Q I ++T+ + +++ + +
Sbjct: 809 RSSENKEHRAETRERAERAEKERRSRAQCQRCKIINRETA--LTRERAHTTHREREARPH 982
Query: 151 STERAKQRNRRITHGEVRFSLTEEENAERVRTRN---PNQR 188
+T R + T+ E+R+S+T+ R + P QR
Sbjct: 983 TTHRIVADEKTPTY-EIRYSVTQSRPRHRATSAQIFAPRQR 1102
>TC87784 weakly similar to PIR|D86292|D86292 protein F7H2.14 [imported] -
Arabidopsis thaliana, partial (15%)
Length = 1138
Score = 33.5 bits (75), Expect = 0.068
Identities = 33/131 (25%), Positives = 57/131 (43%), Gaps = 7/131 (5%)
Frame = +3
Query: 69 NKITENAAPIITETTKKREENHKTLPERKHRASNESKAKTQSKLKKMKRKTQNAG-QIMK 127
+K T + + KR + K+ E K + K + + + T+NA ++M+
Sbjct: 387 SKATATSGYTSNSASTKRCKRKKSFAELKEEETTLLKERIHLE---REIATRNANFEVMR 557
Query: 128 QTSEKIVKKKQKINKKS--KTIFTTSTER----AKQRNRRITHGEVRFSLTEEENAERVR 181
T+E + + K KS K +TS E A Q +RRI EV T+++ +
Sbjct: 558 TTNESLKRMKLGFGSKSHSKPSCSTSVELRGTLAGQPHRRIVPSEVLTCATQDDTHSQAS 737
Query: 182 TRNPNQRENGG 192
PN+ E+ G
Sbjct: 738 ESRPNRIESTG 770
>CA991348 similar to PIR|D84685|D84 hypothetical protein At2g28480 [imported]
- Arabidopsis thaliana, partial (52%)
Length = 658
Score = 33.1 bits (74), Expect = 0.089
Identities = 23/81 (28%), Positives = 39/81 (47%), Gaps = 1/81 (1%)
Frame = +1
Query: 78 IITETTKKREENHKTLPERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKK 137
++ + K + E H+ RK + + + K Q K +K KRK N + ++ K KKK
Sbjct: 43 LVIKDGKTKFETHEVEAPRKDKWKTKKRLKMQRKREKEKRKAANR-RDPRRLGVKGKKKK 219
Query: 138 QKI-NKKSKTIFTTSTERAKQ 157
QK N + + F + R K+
Sbjct: 220 QKFANPEERIKFKINNARVKE 282
>TC82135
Length = 955
Score = 33.1 bits (74), Expect = 0.089
Identities = 39/146 (26%), Positives = 59/146 (39%)
Frame = -3
Query: 51 NNHKIQRNEQKYTRNSKINKITENAAPIITETTKKREENHKTLPERKHRASNESKAKTQS 110
N I R + +RN +I K+ E A +T NH TL R+ R + KTQS
Sbjct: 422 NRIAIART*EIKSRNREIQKL-EIAIDEVT--------NHGTLEMREKR*ERKFAIKTQS 270
Query: 111 KLKKMKRKTQNAGQIMKQTSEKIVKKKQKINKKSKTIFTTSTERAKQRNRRITHGEVRFS 170
QI K+TSE +K + + K+S+ + ++ NR+
Sbjct: 269 -------------QIEKRTSESQLKSE*RSMKRSRKKGIQDEKFIEKTNRK--------- 156
Query: 171 LTEEENAERVRTRNPNQRENGGVREN 196
+N R + R +E VREN
Sbjct: 155 ----QNGSREKVREKKTKERN*VREN 90
>TC76698 similar to PIR|S50766|S50766 dehydrin-related protein - garden pea,
partial (80%)
Length = 974
Score = 31.2 bits (69), Expect = 0.34
Identities = 20/82 (24%), Positives = 42/82 (50%), Gaps = 8/82 (9%)
Frame = +2
Query: 69 NKITENAAPIITETTKKRE--ENHKTLPERKHRASNESKAKTQSKL------KKMKRKTQ 120
+K+T AP T+ +K E + H +L E+ HR+ + S + ++ ++ KK K K +
Sbjct: 242 HKVTLYEAPSETKVEEKEEGEKKHTSLLEKLHRSDSSSSSSSEEEVDGEKRKKKKKEKKE 421
Query: 121 NAGQIMKQTSEKIVKKKQKINK 142
+ +++ +KK ++K
Sbjct: 422 DTSVAVEKVDGTTEEKKGFLDK 487
>TC81657 weakly similar to PIR|T51957|T51957 metalloproteinase (EC 3.4.24.-)
[imported] - Arabidopsis thaliana, partial (24%)
Length = 537
Score = 30.8 bits (68), Expect = 0.44
Identities = 19/76 (25%), Positives = 35/76 (46%), Gaps = 9/76 (11%)
Frame = -3
Query: 6 SKRLNNEHDEKFTVATLRTLFFFFPFQNTDLEKTEDQASNACKGNNNH---------KIQ 56
++R+ N + K+TVA R +FP+ L K + + N+H K Q
Sbjct: 514 TQRMKNVNSTKYTVAVSRIRIIYFPYSV*ILSKYQSTTNKIKTRENSHLQTQKTKPKKHQ 335
Query: 57 RNEQKYTRNSKINKIT 72
R +Q++ +S +I+
Sbjct: 334 RQKQRHPMSSTKTRIS 287
>TC79994 weakly similar to GP|14423560|gb|AAK62462.1 Unknown protein
{Arabidopsis thaliana}, partial (31%)
Length = 1121
Score = 30.4 bits (67), Expect = 0.58
Identities = 10/46 (21%), Positives = 28/46 (60%)
Frame = -1
Query: 37 EKTEDQASNACKGNNNHKIQRNEQKYTRNSKINKITENAAPIITET 82
+K + + +C+GN ++ RNE++ ++ + + T+N A +++ +
Sbjct: 335 QKEKHKGKESCQGNKSNCPNRNEKERSKGRALTRPTKNTASVVSRS 198
>TC76342 similar to PIR|A86444|A86444 probable RNA helicase [imported] -
Arabidopsis thaliana, partial (80%)
Length = 1651
Score = 30.4 bits (67), Expect = 0.58
Identities = 12/40 (30%), Positives = 23/40 (57%)
Frame = +3
Query: 85 KREENHKTLPERKHRASNESKAKTQSKLKKMKRKTQNAGQ 124
K+ ++ +T P+RKH+ + T+S+ K K+K N +
Sbjct: 150 KKNKHEETTPKRKHQDETNLQNDTESEKKSKKKKKHNKSE 269
>TC78017 similar to GP|16648718|gb|AAL25551.1 AT5g64130/MHJ24_11
{Arabidopsis thaliana}, partial (67%)
Length = 887
Score = 30.0 bits (66), Expect = 0.76
Identities = 25/94 (26%), Positives = 46/94 (48%), Gaps = 2/94 (2%)
Frame = -3
Query: 17 FTVATLRT--LFFFFPFQNTDLEKTEDQASNACKGNNNHKIQRNEQKYTRNSKINKITEN 74
+T+ T+ L FPFQN ++++ E + N NH ++ QK R++ + +
Sbjct: 768 YTMTTIHH*FLIVLFPFQNYEIKRYEGRGEEE*NTNQNH--SKSLQKNCRSNNL-----S 610
Query: 75 AAPIITETTKKREENHKTLPERKHRASNESKAKT 108
A II T EN+K L +++ +N+ K+
Sbjct: 609 YANII*IQTSFIVENNKKLYLMRYQNNNQHIIKS 508
>TC79345 similar to GP|21537136|gb|AAM61477.1 putative phosphatidate
phosphohydrolase {Arabidopsis thaliana}, partial (35%)
Length = 1222
Score = 29.6 bits (65), Expect = 0.99
Identities = 16/41 (39%), Positives = 19/41 (46%)
Frame = -1
Query: 148 FTTSTERAKQRNRRITHGEVRFSLTEEENAERVRTRNPNQR 188
F TST KQR ++ G SL E N +RT N R
Sbjct: 1003 FLTSTPSGKQRQKKSGRGRPTASLIESVNTPVIRTANRRPR 881
>TC92460 similar to GP|22202705|dbj|BAC07363. hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (9%)
Length = 795
Score = 29.6 bits (65), Expect = 0.99
Identities = 37/145 (25%), Positives = 51/145 (34%), Gaps = 4/145 (2%)
Frame = +3
Query: 86 REENHKTLPERKH--RASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKKQKINKK 143
R+ H R+H R SK T K RK+ +K S K + K
Sbjct: 246 RDYEHDRHQHRRHHHRTEASSKKSTDPVTKSTSRKSSEPD--IKSKSRKSSEPVTKSLSL 419
Query: 144 SKTIFTTSTERAKQRNRRITHGEVRFSLTEEENAERVRTRNPNQRENGGVRENGNVNLHS 203
SKT TTS E A FS R P++ E + ++ S
Sbjct: 420 SKTAQTTSAEAAAAAAADRKQKASVFS----------RISFPSEEEAAAAAKKRKLSASS 569
Query: 204 SLSYKSAVCVSVSDE--SVTHCTEG 226
+ +A SVS + S TH + G
Sbjct: 570 TTEASTAATASVSAKAPSTTHLSNG 644
>BI271286 similar to PIR|T48519|T485 hypothetical protein F15N18.190 -
Arabidopsis thaliana, partial (31%)
Length = 620
Score = 29.3 bits (64), Expect = 1.3
Identities = 25/91 (27%), Positives = 39/91 (42%), Gaps = 9/91 (9%)
Frame = +2
Query: 65 NSKINKITENAAPIITETTKKREENHKTLPERKH---------RASNESKAKTQSKLKKM 115
+SK T+ T TTK RE +LP +KH R ES +K +
Sbjct: 254 SSKTISKTKTKTKTKTTTTKVRERKVYSLPGQKHDPPEEKEPLRIFYESLSKQIPTSEMA 433
Query: 116 KRKTQNAGQIMKQTSEKIVKKKQKINKKSKT 146
+ G + Q ++K +KKQ+ K+ +T
Sbjct: 434 EFWLMEHGLLSPQKAKKAFEKKQRKQKELRT 526
>TC76866 similar to GP|2909420|emb|CAA12026.1 LEA protein {Cicer arietinum},
partial (85%)
Length = 781
Score = 28.9 bits (63), Expect = 1.7
Identities = 16/65 (24%), Positives = 29/65 (44%)
Frame = +2
Query: 67 KINKITENAAPIITETTKKREENHKTLPERKHRASNESKAKTQSKLKKMKRKTQNAGQIM 126
K N++ N +K ++ + E+ + + +K KTQ + K KTQ A Q
Sbjct: 131 KSNQMMGNIGDKAQAAKEKVQQTAQAAKEKTGQTAQAAKEKTQETAQAAKDKTQQAAQAT 310
Query: 127 KQTSE 131
K ++
Sbjct: 311 KDKTQ 325
Score = 26.6 bits (57), Expect = 8.4
Identities = 28/131 (21%), Positives = 50/131 (37%), Gaps = 15/131 (11%)
Frame = +2
Query: 41 DQASNACKGNNNHKIQRNEQKYTRNSKINKITENAAPIITETTKKREENHKTLPERKHRA 100
++ SN GN K Q ++K + ++ K +K + + E+
Sbjct: 125 EEKSNQMMGNIGDKAQAAKEKVQQTAQAAK-------------EKTGQTAQAAKEKTQET 265
Query: 101 SNESKAKTQSKLKKMKRKTQNA-----------GQIMKQTSEKIVKKK----QKINKKSK 145
+ +K KTQ + K KTQ+ GQ K+T++ Q+ +K K
Sbjct: 266 AQAAKDKTQQAAQATKDKTQDTTGQARDKGYEMGQATKETAQSGKDNSAGFLQQTGEKVK 445
Query: 146 TIFTTSTERAK 156
+ +TE K
Sbjct: 446 GMAQGATEAVK 478
>TC82482 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3L24_8
{Arabidopsis thaliana}, partial (17%)
Length = 672
Score = 28.9 bits (63), Expect = 1.7
Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 3/83 (3%)
Frame = +3
Query: 97 KHRASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKKQKINKKSKTIFTTSTERA- 155
K S+ SKA SK K+ RKT N I ++ S K K + IF + A
Sbjct: 15 KELESDVSKAIVDSKPKRGSRKTSNQLTITEEASSAKKKPKLVTGSTVQIIFGSFLGFAG 194
Query: 156 --KQRNRRITHGEVRFSLTEEEN 176
K+ N + V F++ +EN
Sbjct: 195 TLKKLNSKTKMATVHFTMFGKEN 263
>TC92202 similar to GP|14596161|gb|AAK68808.1 Unknown protein {Arabidopsis
thaliana}, partial (20%)
Length = 653
Score = 28.5 bits (62), Expect = 2.2
Identities = 27/115 (23%), Positives = 45/115 (38%)
Frame = +1
Query: 100 ASNESKAKTQSKLKKMKRKTQNAGQIMKQTSEKIVKKKQKINKKSKTIFTTSTERAKQRN 159
A + K S+ K RK+ + Q +++ ++K+ K S+ K+R
Sbjct: 1 AERKRDTKKTSRKKWKSRKSDLSAQEASTENDEEAERKRDTKKTSR----------KKRK 150
Query: 160 RRITHGEVRFSLTEEENAERVRTRNPNQRENGGVRENGNVNLHSSLSYKSAVCVS 214
++ E S +E AER R R+ +R +LH S VC S
Sbjct: 151 SDLSAQEA--STENDEEAERKRDTKKTSRKKLEIRSECTGSLH*KCSL*KCVCKS 309
>TC89852 similar to GP|15620063|gb|AAL03490.1 unknown {Rickettsia conorii},
partial (22%)
Length = 1001
Score = 28.5 bits (62), Expect = 2.2
Identities = 28/139 (20%), Positives = 57/139 (40%), Gaps = 11/139 (7%)
Frame = +1
Query: 38 KTEDQASNACKGNNNHKIQ-RNEQKYTRNSKIN---------KITENAAPIITETTKKRE 87
K +D S K +N + + R++ K +N+K N +TEN K E
Sbjct: 487 KVKDDLSTDAKVDNGVETEKRSKHKKKKNNKSNIEGGAIEQNAVTENKVKDDLSIDAKVE 666
Query: 88 ENHKTLPERKHRASNESKAKTQSKLKKMKRKTQNAG-QIMKQTSEKIVKKKQKINKKSKT 146
+T KH+ + K+ ++ K+ + + + + K+K+ I+K++
Sbjct: 667 NGAETEKRSKHKMKKKDKSISEGDAKEQIGDPDATNEEAIPEEKKDSKKRKRPISKENDE 846
Query: 147 IFTTSTERAKQRNRRITHG 165
T + + R+I +G
Sbjct: 847 QDTEMKVDEETKRRKIENG 903
>AJ499434 similar to PIR|B86197|B861 hypothetical protein [imported] -
Arabidopsis thaliana, partial (9%)
Length = 506
Score = 28.5 bits (62), Expect = 2.2
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -2
Query: 95 ERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQ 128
+RKH +SKA K K+++R+ +N I+ Q
Sbjct: 379 KRKHTLKRKSKASNCKKTKRVEREMENNNNIVSQ 278
>CB893400 similar to PIR|B86197|B861 hypothetical protein [imported] -
Arabidopsis thaliana, partial (34%)
Length = 565
Score = 28.5 bits (62), Expect = 2.2
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 95 ERKHRASNESKAKTQSKLKKMKRKTQNAGQIMKQ 128
+RKH +SKA K K+++R+ +N I+ Q
Sbjct: 326 KRKHTLKRKSKASNCKKTKRVEREMENNNNIVSQ 427
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.307 0.122 0.323
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,650,516
Number of Sequences: 36976
Number of extensions: 62778
Number of successful extensions: 493
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 485
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 492
length of query: 227
length of database: 9,014,727
effective HSP length: 93
effective length of query: 134
effective length of database: 5,575,959
effective search space: 747178506
effective search space used: 747178506
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0031.12