BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0030.6
(780 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90930 similar to GP|1742955|emb|CAA96058.1 CLC-b chloride chan... 299 3e-83
TC88638 similar to PIR|T02939|T02939 chloride channel protein Cl... 277 1e-74
AW774309 similar to GP|1742957|emb CLC-c chloride channel protei... 187 5e-60
TC82628 similar to GP|1742959|emb|CAA96065.1 CLC-d chloride chan... 132 7e-31
TC81091 similar to GP|4768916|gb|AAD29679.1| CLC-Nt2 protein {Ni... 108 6e-24
TC84010 similar to GP|1742959|emb|CAA96065.1 CLC-d chloride chan... 43 5e-04
BQ752404 31 1.6
CB894538 similar to GP|21553811|gb| outer membrane lipoprotein-l... 31 2.1
TC86634 similar to GP|9759532|dbj|BAB10998.1 outer membrane lipo... 31 2.1
AW689576 similar to GP|7940277|gb| F2401.8 {Arabidopsis thaliana... 30 4.6
TC78393 similar to PIR|T48059|T48059 ABC transporter-like protei... 29 6.0
BQ148506 similar to PIR|T05953|T05 polyphosphoinositide binding ... 29 7.9
CB892643 similar to PIR|B96582|B96 hypothetical protein F15I1.21... 29 7.9
>TC90930 similar to GP|1742955|emb|CAA96058.1 CLC-b chloride channel protein
{Arabidopsis thaliana}, partial (35%)
Length = 857
Score = 299 bits (765), Expect(2) = 3e-83
Identities = 144/257 (56%), Positives = 195/257 (75%), Gaps = 2/257 (0%)
Frame = +1
Query: 300 KGGLIMFDAYSGSLMYHAKDVPLVFILGVIGGILGSLYNYLWNKVLRIYNAINEKGTICK 359
+ G IMFD + ++ YH D+ V ++G+IGG+LGSLYN+L +KVLR+YN IN+KG + K
Sbjct: 34 QAGXIMFDVSNVTVRYHVMDILPVAVIGIIGGLLGSLYNHLLHKVLRLYNLINQKGKMHK 213
Query: 360 ILLACVISMFTSCLLFGLPWLASCQPC-PADPV-EPCPTIGRSGIYKKFQCPPGHYNDLA 417
+LL+ + +FTS +GLP+LA C PC P+ P E CPT GRSG +K+F CPPGHYNDLA
Sbjct: 214 LLLSLAVVLFTSACQYGLPFLAKCTPCNPSLPESELCPTNGRSGNFKQFNCPPGHYNDLA 393
Query: 418 SLIFNTNDDAIRNLFSKNTDSEFQYSSMFIFFITGFFLSILSCGVVVPAGVFVPIIVTGA 477
+L+ TNDDA+RN+FS NT E+Q SS+ IFF L +++ G+ VP G+F+PII+ G+
Sbjct: 394 TLLLTTNDDAVRNIFSSNTPHEYQTSSLLIFFALYCILGLITFGIAVPTGLFLPIILMGS 573
Query: 478 SYGRFVGMLFGKKSNLNHGLYAVLGAASFLGGSMRTTVSLCVIILELTNNLLLLPLIMMV 537
+YGR VGML G + ++ GL+AVLGAAS + GSMR TVSLCVI LELT+NLLLLP+ M+V
Sbjct: 574 AYGRLVGMLMGPHTKIDQGLFAVLGAASLMAGSMRMTVSLCVIFLELTSNLLLLPITMIV 753
Query: 538 LLVSKSVADVFNANIYD 554
LL++K+V D FN +IY+
Sbjct: 754 LLIAKTVGDCFNPSIYE 804
Score = 28.9 bits (63), Expect(2) = 3e-83
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +3
Query: 290 CLSGKCGLFGKGG 302
C SGKCGLFG GG
Sbjct: 3 CNSGKCGLFGAGG 41
>TC88638 similar to PIR|T02939|T02939 chloride channel protein ClC-1 -
common tobacco, partial (29%)
Length = 1585
Score = 277 bits (709), Expect = 1e-74
Identities = 132/222 (59%), Positives = 169/222 (75%)
Frame = +3
Query: 17 QPLLSSQRSIINSSSQVAIVGANVCPIESLDYEIFENEFFKQDWRSRGSLQIFQYVLMKW 76
+PLL + IN++SQ+AIVG+N+CPIESLDYE+F+NE F QDWRSR +QIFQYV++KW
Sbjct: 468 EPLLVKR---INTTSQIAIVGSNLCPIESLDYEVFDNEMFNQDWRSRKRVQIFQYVVLKW 638
Query: 77 LLCFLIGTILGLIGFCNNLAVENLAGVKFVVTSNMMLHKRFSLAFVTFFASNLALTLFAT 136
+ LIG GL+G NN++VEN+AG K +T+++M +RF AF+ + N+ L A
Sbjct: 639 VFALLIGLGTGLVGLFNNISVENIAGFKLTLTTDLMSKQRFFEAFLAYAGLNMVLAAAAA 818
Query: 137 IITAMIAPAAAGSGISEVKAYLNGVDAPGIFTLPTLIVKIIGSITAVSSSLHIGKAGPML 196
+ A IAP+AAGSGI EVKAYLNG+DA I TL VKI+GSI VS+ +GK GPM+
Sbjct: 819 ALCAFIAPSAAGSGIPEVKAYLNGIDAHSILAPTTLFVKIVGSILGVSAGFVVGKEGPMV 998
Query: 197 HTGACVAALLGQGGSKRYGLTWKWLRYFKNDRDRRDLIVCGS 238
HTGAC+A++LGQGGSK+YGLTW WLRYFKNDRDRRD+I C S
Sbjct: 999 HTGACIASILGQGGSKKYGLTWSWLRYFKNDRDRRDMISCRS 1124
Score = 32.0 bits (71), Expect = 0.93
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +2
Query: 738 TRHDFTAEHILGTHPYLVRSRW 759
TRHDF EH+LG +P + +W
Sbjct: 1100 TRHDFMPEHVLGLYPDIKHHKW 1165
>AW774309 similar to GP|1742957|emb CLC-c chloride channel protein
{Arabidopsis thaliana}, partial (22%)
Length = 658
Score = 187 bits (476), Expect(2) = 5e-60
Identities = 85/142 (59%), Positives = 107/142 (74%)
Frame = +1
Query: 377 LPWLASCQPCPADPVEPCPTIGRSGIYKKFQCPPGHYNDLASLIFNTNDDAIRNLFSKNT 436
LPW+A+C PCP D PCP++ SG YK FQCPPG+YNDLASL NTNDDAIRNLFS
Sbjct: 232 LPWIANCIPCPTDSKVPCPSVDESGEYKIFQCPPGYYNDLASLFLNTNDDAIRNLFSPKI 411
Query: 437 DSEFQYSSMFIFFITGFFLSILSCGVVVPAGVFVPIIVTGASYGRFVGMLFGKKSNLNHG 496
EF SS+FIFF T +FL IL+ G+ VP+G+F+P+I+ GA+YGR V LF + L+ G
Sbjct: 412 TKEFHISSLFIFFATVYFLGILTYGIAVPSGLFIPVILAGAAYGRVVSRLFEPITQLDRG 591
Query: 497 LYAVLGAASFLGGSMRTTVSLC 518
+++LGAAS LGG+MR TVS+C
Sbjct: 592 FFSLLGAASMLGGTMRMTVSIC 657
Score = 62.8 bits (151), Expect(2) = 5e-60
Identities = 31/63 (49%), Positives = 41/63 (64%)
Frame = +3
Query: 315 YHAKDVPLVFILGVIGGILGSLYNYLWNKVLRIYNAINEKGTICKILLACVISMFTSCLL 374
+ A D+ V LG + GIL S+YN+L +KV+R Y+ INEKG KI LA IS+ TSC
Sbjct: 45 FSAGDIVAVIGLGTVAGILASIYNFLVDKVVRTYSIINEKGPFFKIFLAVAISLLTSCCY 224
Query: 375 FGL 377
+ L
Sbjct: 225 YFL 233
>TC82628 similar to GP|1742959|emb|CAA96065.1 CLC-d chloride channel protein
{Arabidopsis thaliana}, partial (21%)
Length = 881
Score = 132 bits (331), Expect = 7e-31
Identities = 77/157 (49%), Positives = 105/157 (66%), Gaps = 5/157 (3%)
Frame = +1
Query: 594 KVRNVVFVLKTTSHNGFPVIDEPPFSEAPVLFGIILRHHLLTLLRKKV-FMSAPMAM--- 649
KV +VV +L++ +HNGFPVID E P++ G++LR HLL +L+ KV F +P+
Sbjct: 64 KVSDVVSILQSNTHNGFPVIDHTRSGE-PLVIGLVLRSHLLVILQSKVDFQHSPLPSDPR 240
Query: 650 -GGDVLRQFSADDFAKRGSDRGERIEDIHLTQEEMDMFIDLHPFTNASPYTVVESMSLGK 708
GG +R S + FAK S +G ++DIHLT E+++M+IDL PF N SPY V E MSL K
Sbjct: 241 TGGRSIRHDSGE-FAKPVSSKGICLDDIHLTSEDLEMYIDLAPFLNPSPYIVPEDMSLTK 417
Query: 709 ALILFRELGLRHLLVIPKIPSRSPVVGILTRHDFTAE 745
LFR+LGLRHL V+P+ PSR V+G++TR D E
Sbjct: 418 VYNLFRQLGLRHLFVVPR-PSR--VLGLITRKDLLIE 519
>TC81091 similar to GP|4768916|gb|AAD29679.1| CLC-Nt2 protein {Nicotiana
tabacum}, partial (13%)
Length = 662
Score = 108 bits (271), Expect = 6e-24
Identities = 51/99 (51%), Positives = 77/99 (77%), Gaps = 2/99 (2%)
Frame = +3
Query: 667 SDRGERIEDIHLTQEEMDMFIDLHPFTNASPYTVVESMSLGKALILFRELGLRHLLVIPK 726
++R +IE++ +T+EEM+MF+DLHP TN +P+TV+ES+S+ KA+ILFR++GLRHLLV+PK
Sbjct: 39 AEREGKIEEVAITREEMEMFVDLHPLTNTTPFTVLESISVAKAMILFRQVGLRHLLVVPK 218
Query: 727 IPSRS--PVVGILTRHDFTAEHILGTHPYLVRSRWKRLR 763
+ PV+GILTR D A +IL P+L +S+ ++ R
Sbjct: 219 YQASGVCPVIGILTRQDLLAYNILTVFPHLAKSKNRQKR 335
>TC84010 similar to GP|1742959|emb|CAA96065.1 CLC-d chloride channel protein
{Arabidopsis thaliana}, partial (12%)
Length = 589
Score = 42.7 bits (99), Expect = 5e-04
Identities = 29/99 (29%), Positives = 46/99 (46%), Gaps = 4/99 (4%)
Frame = +1
Query: 1 MSTNHLSNGDSEHRLRQPLL--SSQRSIINSSSQVAIVGANV-CPIESLDYEIFENEFFK 57
M +NH+ NG RL + S + + V I+ N +ESLDYE+ EN ++
Sbjct: 163 MLSNHIQNGIESARLAWSRIPNSDESPFVLDDDGVGILKKNDGSAVESLDYEVIENFAYR 342
Query: 58 QDW-RSRGSLQIFQYVLMKWLLCFLIGTILGLIGFCNNL 95
++ R L + +++KW LIG GL N+
Sbjct: 343 EEQAHRRRKLYVSYLLVVKWFFALLIGICTGLAAVFINI 459
>BQ752404
Length = 613
Score = 31.2 bits (69), Expect = 1.6
Identities = 25/95 (26%), Positives = 44/95 (46%), Gaps = 1/95 (1%)
Frame = +2
Query: 242 IAAAFRAPVGGVLFALEEMASWWRTALLW-RAFFTTAIVAIFLRAMIDVCLSGKCGLFGK 300
I + F +G L+ALE + W+R L W +A A++ + L A+ VC + F
Sbjct: 8 ITSVFTLDLG--LYALENLRVWFRLDLAWLQARTVGALLMLALLALGSVCSASGVYRFAL 181
Query: 301 GGLIMFDAYSGSLMYHAKDVPLVFILGVIGGILGS 335
+M + + + LV +LG + G +G+
Sbjct: 182 VAFVMRHSIRSKFVARVSERILV-LLGRVSGTVGT 283
>CB894538 similar to GP|21553811|gb| outer membrane lipoprotein-like
{Arabidopsis thaliana}, partial (20%)
Length = 774
Score = 30.8 bits (68), Expect = 2.1
Identities = 16/36 (44%), Positives = 22/36 (60%), Gaps = 6/36 (16%)
Frame = +2
Query: 76 WLLCFLIGTILGLIGFCN------NLAVENLAGVKF 105
+L CFL +LG+IGFC L ++NLAG+ F
Sbjct: 2 FLQCFLSFLLLGIIGFCTLIMIIIMLLLDNLAGIIF 109
>TC86634 similar to GP|9759532|dbj|BAB10998.1 outer membrane
lipoprotein-like {Arabidopsis thaliana}, partial (95%)
Length = 857
Score = 30.8 bits (68), Expect = 2.1
Identities = 16/36 (44%), Positives = 22/36 (60%), Gaps = 6/36 (16%)
Frame = +3
Query: 76 WLLCFLIGTILGLIGFCN------NLAVENLAGVKF 105
+L CFL +LG+IGFC L ++NLAG+ F
Sbjct: 312 FLQCFLSFLLLGIIGFCTLIMIIIMLLLDNLAGIIF 419
>AW689576 similar to GP|7940277|gb| F2401.8 {Arabidopsis thaliana}, partial
(45%)
Length = 686
Score = 29.6 bits (65), Expect = 4.6
Identities = 18/49 (36%), Positives = 28/49 (56%), Gaps = 4/49 (8%)
Frame = -3
Query: 421 FNTNDDAIRNLFSKNTDSEFQYSSMFIFFI----TGFFLSILSCGVVVP 465
FN+ D+ + + NTD+ Y+ F FF+ T FF+S+LS +VP
Sbjct: 231 FNSYDN*TQKI-KHNTDTIVFYTVFFYFFLFPSYTHFFVSLLSSTTIVP 88
>TC78393 similar to PIR|T48059|T48059 ABC transporter-like protein -
Arabidopsis thaliana, partial (12%)
Length = 817
Score = 29.3 bits (64), Expect = 6.0
Identities = 39/171 (22%), Positives = 66/171 (37%), Gaps = 4/171 (2%)
Frame = +2
Query: 444 SMFIFFITGFFLSILSCGVVVPAGVFVPIIVTGASYGRFVGMLFGKKSNLNHGLYAVLGA 503
S F F L+++SC VV+ F S FVGML + H +
Sbjct: 62 SSFTFITDSAVLAVVSCIVVMARAFFFTYWGLKTSQSCFVGML----QRILHAPMSFFDT 229
Query: 504 ASFLGGSMRTTVSLCVIILELTNNLLLLPLIMMVLLVSKSVADVFNANIYDLIMKSKGLP 563
G + + VS ++ L++ + + L+ L+ S+ V N ++ + L
Sbjct: 230 TP--SGRILSRVSTDILSLDI-QIPIFVNLVTTTLVGLFSILVVTCQNSWETVFLLIPLF 400
Query: 564 YLETHAEPY----MRQLTVGDVVTGPLQIFHGYEKVRNVVFVLKTTSHNGF 610
+L Y R+LT D +T I H E + V+ + + N F
Sbjct: 401 WLSNWYRKYYLATSRELTRLDSITKAPVIHHFSETISGVMTIRSSRKQNAF 553
>BQ148506 similar to PIR|T05953|T05 polyphosphoinositide binding protein Ssh2
- soybean, partial (69%)
Length = 676
Score = 28.9 bits (63), Expect = 7.9
Identities = 18/56 (32%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = -2
Query: 71 YVLMKWLLCFLIG---TILGLIGFCNNLAVENLAGVK-FVVTSNMMLHKRFSLAFV 122
++L +W+LCF G T G FC+N+ L F++T+ H + +L FV
Sbjct: 192 FLLRRWILCFNKGSHKTDFG*FSFCDNIICNYLCFCF*FLMTTLHCFHFQLTLFFV 25
>CB892643 similar to PIR|B96582|B96 hypothetical protein F15I1.21 [imported]
- Arabidopsis thaliana, partial (35%)
Length = 758
Score = 28.9 bits (63), Expect = 7.9
Identities = 29/92 (31%), Positives = 43/92 (46%), Gaps = 11/92 (11%)
Frame = +2
Query: 462 VVVPAGVFVPIIVTGASYGRFVGMLFGKKSNLNHGLYAVLGAASFLGGSMRTTVSLCV-- 519
V++P G P + AS FVG G+ GL +VLG A F+ + TVSLCV
Sbjct: 275 VLLPLGNGAPDVF--ASIASFVGTDTGEV-----GLNSVLGGALFVTTVVVGTVSLCVAE 433
Query: 520 ---------IILELTNNLLLLPLIMMVLLVSK 542
I +L+ L + ++++L V K
Sbjct: 434 RDVQVDRRCFIRDLSFFLFTIFSLLLILFVGK 529
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.327 0.142 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,200,259
Number of Sequences: 36976
Number of extensions: 405488
Number of successful extensions: 2959
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 2165
Number of HSP's successfully gapped in prelim test: 99
Number of HSP's that attempted gapping in prelim test: 743
Number of HSP's gapped (non-prelim): 2323
length of query: 780
length of database: 9,014,727
effective HSP length: 104
effective length of query: 676
effective length of database: 5,169,223
effective search space: 3494394748
effective search space used: 3494394748
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0030.6