BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0030.13
(117 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93121 weakly similar to GP|8953375|emb|CAB96648.1 putative pro... 175 2e-45
TC78599 similar to GP|9758008|dbj|BAB08605.1 contains similarity... 86 1e-21
TC83137 similar to GP|9758523|dbj|BAB08970.1 contains similarity... 91 5e-20
TC88262 homologue to GP|15293111|gb|AAK93666.1 unknown protein {... 82 4e-17
TC82546 similar to PIR|T04446|T04446 hypothetical protein F4D11.... 74 1e-14
TC79742 similar to GP|10177241|dbj|BAB10615. gb|AAC98455.1~gene_... 47 1e-06
BE204106 homologue to GP|15809826|gb AT5g61840/mac9_140 {Arabido... 42 3e-05
BE317779 similar to GP|19347795|gb| unknown protein {Arabidopsis... 41 8e-05
TC86908 similar to PIR|G96708|G96708 hypothetical protein T26J14... 33 0.012
TC92141 similar to PIR|F85056|F85056 hypothetical protein AT4g04... 27 0.88
TC76685 similar to PIR|A84912|A84912 probable galactinol synthas... 27 1.5
AW126373 similar to GP|15144505|gb| unknown {Lycopersicon escule... 27 1.5
AW256806 27 1.5
TC92278 similar to SP|O43093|KINH_SYNRA Kinesin heavy chain (Syn... 26 2.0
TC86210 homologue to GP|13194621|gb|AAK15493.1 brassinosteroid b... 26 2.6
TC90190 similar to GP|3426041|gb|AAC32240.1| unknown protein {Ar... 25 3.3
AW688366 25 3.3
CB894770 homologue to GP|4512698|gb| unknown protein {Arabidopsi... 25 4.4
CB894792 weakly similar to GP|19309913|emb Mob1-like protein {Me... 25 5.7
BG584375 similar to GP|6498442|dbj ESTs AU081301(E20138) C99280(... 25 5.7
>TC93121 weakly similar to GP|8953375|emb|CAB96648.1 putative protein
{Arabidopsis thaliana}, partial (27%)
Length = 918
Score = 175 bits (444), Expect = 2e-45
Identities = 78/117 (66%), Positives = 96/117 (81%)
Frame = +1
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPF 60
MVKR KVWVY+EGEQPLVHDGP+ YSIEGQF+DE+D+ KSPFKA HP+ A++F +PF
Sbjct: 367 MVKRFKVWVYKEGEQPLVHDGPVNNKYSIEGQFIDEMDTSNKSPFKATHPELAHVFFLPF 546
Query: 61 SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
S+S +++YVY P S+ DY+ RLQ LVEDY+ +VA+KYPYWN S GAD FLLSCHD
Sbjct: 547 SVSKVIRYVYKPRKSRSDYNPHRLQLLVEDYIKIVANKYPYWNISQGADQFLLSCHD 717
>TC78599 similar to GP|9758008|dbj|BAB08605.1 contains similarity to
limonene cyclase~gene_id:MED24.9 {Arabidopsis thaliana},
partial (85%)
Length = 1857
Score = 85.9 bits (211), Expect(2) = 1e-21
Identities = 36/88 (40%), Positives = 62/88 (69%)
Frame = +3
Query: 30 EGQFMDEIDSCRKSPFKARHPDEANLFLIPFSISNIVQYVYMPILSQQDYHLGRLQRLVE 89
+G F+ I+ F+ R P++A++F +PFS++ +VQ+VY+ + + +++ +
Sbjct: 693 KGNFIHAIEM--NDRFRTRDPEKAHVFFLPFSVAKMVQFVYV----RDSHDFSPIRKTIT 854
Query: 90 DYVHVVADKYPYWNRSNGADHFLLSCHD 117
DY++VV++KYP+WNRS GADHF+LSCHD
Sbjct: 855 DYINVVSEKYPFWNRSLGADHFMLSCHD 938
Score = 31.2 bits (69), Expect(2) = 1e-21
Identities = 11/23 (47%), Positives = 18/23 (77%)
Frame = +2
Query: 11 EEGEQPLVHDGPLKGLYSIEGQF 33
++GE P+ H+GP K +YS+E +F
Sbjct: 635 KKGEPPVFHNGPCKSIYSMERKF 703
>TC83137 similar to GP|9758523|dbj|BAB08970.1 contains similarity to
limonene cyclase~gene_id:K15O15.2 {Arabidopsis
thaliana}, partial (50%)
Length = 994
Score = 91.3 bits (225), Expect = 5e-20
Identities = 43/117 (36%), Positives = 75/117 (63%)
Frame = +2
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPF 60
M ++LKV++Y EGE+P+ H ++G+Y+ EG FM ++ ++ F + P +A+LF +PF
Sbjct: 149 MERKLKVYIYREGEKPIFHQPKMRGIYASEGWFMKLMEGNKR--FIVKDPKKAHLFYLPF 322
Query: 61 SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
S + LS ++++ ++ YV+++A KY +WNR+ GADHFL++CHD
Sbjct: 323 S-----SQMLRANLSDNK----KMEQYLDKYVNIIAGKYRFWNRTGGADHFLVACHD 466
>TC88262 homologue to GP|15293111|gb|AAK93666.1 unknown protein {Arabidopsis
thaliana}, partial (66%)
Length = 1367
Score = 81.6 bits (200), Expect = 4e-17
Identities = 47/123 (38%), Positives = 65/123 (52%), Gaps = 6/123 (4%)
Frame = +2
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLK--GLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLI 58
M K+ KV++Y +G+ + P K G Y+ EG F I R+S F+ PDEA+LF I
Sbjct: 395 MEKKFKVYIYPDGDSKTFYQTPRKLTGKYASEGYFFQNI---RESRFRTLDPDEAHLFFI 565
Query: 59 PFSISNI----VQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLS 114
P S + Y M I+ V++YV + KYPYWNR+ GADHF ++
Sbjct: 566 PISCHKMRGKGTSYDNMTII-------------VQNYVESLISKYPYWNRTLGADHFFVT 706
Query: 115 CHD 117
CHD
Sbjct: 707 CHD 715
>TC82546 similar to PIR|T04446|T04446 hypothetical protein F4D11.10 -
Arabidopsis thaliana, partial (35%)
Length = 760
Score = 73.6 bits (179), Expect = 1e-14
Identities = 35/103 (33%), Positives = 64/103 (61%)
Frame = +1
Query: 15 QPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPFSISNIVQYVYMPIL 74
+P+ H L G+Y+ EG FM +++ ++ F ++P +A+LF +PFS + + +Y+
Sbjct: 7 RPISHSPFLTGIYASEGWFMKLMEANKR--FVTKNPKKAHLFYLPFSSRMLEEALYV--- 171
Query: 75 SQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
+ + L + + DYV ++A ++ +WNR+ GADHFL+ CHD
Sbjct: 172 -KNSHSHKNLIQYLHDYVDMIAARHSFWNRTGGADHFLVGCHD 297
>TC79742 similar to GP|10177241|dbj|BAB10615.
gb|AAC98455.1~gene_id:MRN17.17~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (32%)
Length = 1063
Score = 47.0 bits (110), Expect = 1e-06
Identities = 34/120 (28%), Positives = 55/120 (45%), Gaps = 4/120 (3%)
Frame = +2
Query: 2 VKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRK---SPFKARHPDEANLFLI 58
+K LK++VY+ P + LK F E+ R S + P EA+ F +
Sbjct: 488 LKNLKIFVYDL--PPKYNKNWLKNPRCKTHLFASEVAIHRALLTSDVRTFDPYEADFFFV 661
Query: 59 PFSIS-NIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
P +S N P + G + L+ V +++ +YP+WNRS G+DH ++ HD
Sbjct: 662 PVYVSCNFSTVNGFPAI-------GHARSLISSAVKLISTEYPFWNRSTGSDHVFVASHD 820
>BE204106 homologue to GP|15809826|gb AT5g61840/mac9_140 {Arabidopsis
thaliana}, partial (44%)
Length = 555
Score = 42.4 bits (98), Expect = 3e-05
Identities = 25/91 (27%), Positives = 42/91 (45%), Gaps = 14/91 (15%)
Frame = +2
Query: 41 RKSPFKARHPDEANLFLIPFSISNIVQ--------YVYMPILSQQDYHLGRLQ------R 86
+K P H A F+ F +S+ V+ + Y P+ + D L R
Sbjct: 38 QKDPRCLTHMFAAERFMHRFLLSSAVRTLNPEEADWFYTPVYTTCDLTPNGLPLPFKSPR 217
Query: 87 LVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
++ + +++ +PYWNR+ GADHF + HD
Sbjct: 218 MMRSAIQLISSNWPYWNRTEGADHFFVVPHD 310
>BE317779 similar to GP|19347795|gb| unknown protein {Arabidopsis thaliana},
partial (14%)
Length = 295
Score = 40.8 bits (94), Expect = 8e-05
Identities = 16/34 (47%), Positives = 26/34 (76%)
Frame = +1
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFM 34
M K LKV++Y +G++P+ H+ L+G+Y+ EG FM
Sbjct: 154 MEKMLKVYIYADGDRPIFHEPLLEGIYASEGWFM 255
>TC86908 similar to PIR|G96708|G96708 hypothetical protein T26J14.4
[imported] - Arabidopsis thaliana, partial (65%)
Length = 1587
Score = 33.5 bits (75), Expect = 0.012
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +2
Query: 50 PDEANLFLIPFSISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGAD 109
P++A LF IPF ++ + ++ H R V+ HV++ P+WNR NG D
Sbjct: 632 PNQAILFYIPFYGG-----LHASSMFREANHTLRDSLAVDLIDHVLS--LPWWNRHNGKD 790
Query: 110 HFL 112
HF+
Sbjct: 791 HFI 799
>TC92141 similar to PIR|F85056|F85056 hypothetical protein AT4g04480
[imported] - Arabidopsis thaliana, partial (28%)
Length = 797
Score = 27.3 bits (59), Expect = 0.88
Identities = 11/36 (30%), Positives = 22/36 (60%)
Frame = +1
Query: 46 KARHPDEANLFLIPFSISNIVQYVYMPILSQQDYHL 81
K +H + ++IPF + I+ +++ P + QQ Y+L
Sbjct: 13 KTKHTQK---YIIPFKLIFIISWLWPPYIPQQHYYL 111
>TC76685 similar to PIR|A84912|A84912 probable galactinol synthase
[imported] - Arabidopsis thaliana, partial (79%)
Length = 1356
Score = 26.6 bits (57), Expect = 1.5
Identities = 26/105 (24%), Positives = 44/105 (41%), Gaps = 23/105 (21%)
Frame = +1
Query: 4 RLKVWVYEEGEQPLVHDGPLKGLYSIEGQF----------MD-------------EIDSC 40
+L++W +EE ++ + DG ++ +I+ F MD EI C
Sbjct: 394 KLRIWAFEEYDKMIYLDGDIQVFENIDHLFDLPNNYFYAVMDCFCEASWRHTKQYEIGYC 573
Query: 41 RKSPFKARHPDEANLFLIPFSISNIVQYVYMPILSQQDYHLGRLQ 85
++ P K + P AN P N +VY P ++ L +LQ
Sbjct: 574 QQCPDKVQWP--ANFGPKPPLYFNAGMFVYEPNMATYHDLLQKLQ 702
>AW126373 similar to GP|15144505|gb| unknown {Lycopersicon esculentum},
partial (46%)
Length = 480
Score = 26.6 bits (57), Expect = 1.5
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 39 SCRKSPFKARHPDEANLFLIPFSISNIVQYVYMPIL 74
+CR SPF + P +++ V++VYMP+L
Sbjct: 300 TCRSSPFSST--------TFPITVTVTVKFVYMPLL 383
>AW256806
Length = 425
Score = 26.6 bits (57), Expect = 1.5
Identities = 10/35 (28%), Positives = 20/35 (56%)
Frame = -1
Query: 46 KARHPDEANLFLIPFSISNIVQYVYMPILSQQDYH 80
K +PDE L+ +P + ++VQY + + + +H
Sbjct: 329 KGHNPDERGLYNLPTNRDSLVQYSFFILYEKWAHH 225
>TC92278 similar to SP|O43093|KINH_SYNRA Kinesin heavy chain (Synkin).
{Syncephalastrum racemosum}, partial (10%)
Length = 709
Score = 26.2 bits (56), Expect = 2.0
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = -2
Query: 55 LFLIPFSISNIVQYVYMPILSQQDY 79
++L+ +S + QY Y+P+L Q Y
Sbjct: 378 IYLLILLLSQLYQYYYLPLLQQLSY 304
>TC86210 homologue to GP|13194621|gb|AAK15493.1 brassinosteroid biosynthetic
protein LKB {Pisum sativum}, partial (67%)
Length = 1377
Score = 25.8 bits (55), Expect = 2.6
Identities = 14/64 (21%), Positives = 29/64 (44%)
Frame = -1
Query: 23 LKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPFSISNIVQYVYMPILSQQDYHLG 82
L G+YS F+ + +C + HP L PF +++ + Y+P++ +L
Sbjct: 462 LVGMYSTNSPFLSAVSACW*NQVLYHHP--TLFILFPFFLASSFE-AYLPVMHSDPGNLP 292
Query: 83 RLQR 86
+ +
Sbjct: 291 HIDK 280
>TC90190 similar to GP|3426041|gb|AAC32240.1| unknown protein {Arabidopsis
thaliana}, partial (60%)
Length = 1626
Score = 25.4 bits (54), Expect = 3.3
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +2
Query: 102 WNRSNGADHFLLSCHD 117
+NR NG FLLSC+D
Sbjct: 932 YNRENGHAGFLLSCYD 979
>AW688366
Length = 646
Score = 25.4 bits (54), Expect = 3.3
Identities = 9/17 (52%), Positives = 13/17 (75%)
Frame = +1
Query: 24 KGLYSIEGQFMDEIDSC 40
+G+YSI +M+ IDSC
Sbjct: 133 RGIYSIMHHYMENIDSC 183
>CB894770 homologue to GP|4512698|gb| unknown protein {Arabidopsis thaliana},
partial (40%)
Length = 720
Score = 25.0 bits (53), Expect = 4.4
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = +1
Query: 98 KYPYWNRSNGADHFLLS 114
K P W+ NG DHFL++
Sbjct: 79 KRPEWSIMNGKDHFLVA 129
>CB894792 weakly similar to GP|19309913|emb Mob1-like protein {Medicago
sativa subsp. falcata}, partial (13%)
Length = 358
Score = 24.6 bits (52), Expect = 5.7
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = -2
Query: 29 IEGQFMDEIDSCRKSPFKARHPDEANLFLIPFS 61
+ G F CR + F A P +LF +PFS
Sbjct: 183 VGGSFSPPFRGCRPALFLAIFPCFCSLFSLPFS 85
>BG584375 similar to GP|6498442|dbj ESTs AU081301(E20138) C99280(E10593)
correspond to a region of the predicted gene.~Similar
to, partial (39%)
Length = 765
Score = 24.6 bits (52), Expect = 5.7
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +3
Query: 102 WNRSNGADHFLLSCHD 117
+NR NG FLLSC+D
Sbjct: 318 YNRENGHLGFLLSCYD 365
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.142 0.448
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,395,637
Number of Sequences: 36976
Number of extensions: 59499
Number of successful extensions: 298
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 295
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 296
length of query: 117
length of database: 9,014,727
effective HSP length: 93
effective length of query: 24
effective length of database: 5,575,959
effective search space: 133823016
effective search space used: 133823016
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0030.13