BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.13
(195 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 80 5e-16
TC90516 64 1e-12
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 66 1e-11
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 65 1e-11
AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase... 54 5e-08
TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR re... 37 0.005
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 35 0.014
TC82093 32 0.16
TC88690 weakly similar to GP|15724162|gb|AAL06473.1 AT3g59090/F1... 32 0.16
BQ138029 weakly similar to GP|1764098|gb|A putative permease {Ur... 29 1.3
TC78468 similar to GP|21594872|gb|AAM66051.1 unknown {Arabidopsi... 27 3.9
BG451876 weakly similar to GP|15144511|gb| unknown {Lycopersicon... 27 6.6
TC79820 similar to GP|21537325|gb|AAM61666.1 unknown {Arabidopsi... 27 6.6
TC93240 weakly similar to GP|14335170|gb|AAK59865.1 AT5g51070/K3... 27 6.6
BI264438 similar to GP|21427467|gb actin-related protein 6 {Arab... 26 8.6
TC77667 similar to PIR|T48625|T48625 pescadillo-like protein - A... 26 8.6
TC89561 similar to GP|9759196|dbj|BAB09733.1 GTPase activator pr... 26 8.6
TC83218 26 8.6
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 80.1 bits (196), Expect = 5e-16
Identities = 54/180 (30%), Positives = 87/180 (48%), Gaps = 3/180 (1%)
Frame = +3
Query: 10 ELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTKYFHSLI 69
+L K EE L E NE + FW QKSR WL+ GD+NTK+FH++
Sbjct: 57 KLFNKGEELSLLRSELNEEYHNEEIFWM----------QKSRLNWLRSGDRNTKFFHAVT 206
Query: 70 NWRKRTNSIMGLMIDGN--WVEDPQAIKTEVQNYFEQCFTNSLGTLVRLDEISFKSI-SD 126
R+ N I+ L+ D + W + + + ++F+ +++ + D S +I ++
Sbjct: 207 KNRRAQNRILSLIDDDDKEWFVE-EDLGRLADSHFKLLYSSEDVGITLEDWNSIPAIVTE 383
Query: 127 ADNELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFIQKFWDLLKDDFHRVLEDFWNRG 186
N L + EEV+ AV++ K P PD N F Q+FWD + DD + ++F G
Sbjct: 384 EQNAQLMAQISREEVREAVFDINPHKCPGPDGMNVFFFQQFWDTMGDDLTSMAQEFLRTG 563
>TC90516
Length = 983
Score = 63.9 bits (154), Expect(2) = 1e-12
Identities = 39/128 (30%), Positives = 66/128 (51%)
Frame = -2
Query: 56 KEGDQNTKYFHSLINWRKRTNSIMGLMIDGNWVEDPQAIKTEVQNYFEQCFTNSLGTLVR 115
+EG NT YF + + R NSI ++ W++D K + N+F F++
Sbjct: 754 REG-HNTTYFLACVKNRGMRNSISAPRVE-RWMDDVAKNKQAIVNFFTHHFSDP*TYRPT 581
Query: 116 LDEISFKSISDADNELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFIQKFWDLLKDDF 175
+ +I F IS+ DN LL+ +F + +++ V G++SP PD FN F + ++LK D
Sbjct: 580 MGDIDFFHISNLDNVLLSAQFLVSKMELVVSSLDGNESPRPDGFNLNFFIRLRNMLKADI 401
Query: 176 HRVLEDFW 183
+ E F+
Sbjct: 400 EIMFEQFY 377
Score = 25.0 bits (53), Expect(2) = 1e-12
Identities = 10/21 (47%), Positives = 16/21 (75%)
Frame = -1
Query: 37 TVLRLHE*LLYQKSRSKWLKE 57
TVL + + ++Q SR+KWL+E
Sbjct: 815 TVLPIQDSQMFQWSRAKWLRE 753
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 65.9 bits (159), Expect = 1e-11
Identities = 43/113 (38%), Positives = 61/113 (53%), Gaps = 3/113 (2%)
Frame = +2
Query: 48 QKSRSKWLKEGDQNTKYFHSLINWRKRTNSIMGLM-IDGNWVEDPQAIKTEVQNYFEQCF 106
QKSR+ W GD N K++H+L R N I+GL DGNW+ + Q ++ +YFE F
Sbjct: 59 QKSRNMWHISGDLNKKFYHALTKQRHARNRIVGLYDYDGNWITEEQGVEKVAVDYFEDLF 238
Query: 107 --TNSLGTLVRLDEISFKSISDADNELLTGRFGMEEVKGAVWECGGDKSPEPD 157
T G LDEI+ SI+ N+ L EEV+ A++ +K+P PD
Sbjct: 239 QRTTPTGFDGFLDEIT-SSITPQMNQRLLRLATEEEVRLALFIMHPEKAPGPD 394
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 65.5 bits (158), Expect = 1e-11
Identities = 39/128 (30%), Positives = 65/128 (50%), Gaps = 2/128 (1%)
Frame = +2
Query: 54 WLKEGDQNTKYFHSLINWRKRTNSIMGLMID-GNWVEDPQAIKTEVQNYFEQCFTNSLGT 112
WLK+GD+NTK+FHS + R++ N I L + GNW + + ++ + YF FT+S T
Sbjct: 5 WLKDGDKNTKFFHSKASQRRKVNEIKKLKDETGNWCKGEENVERLLITYFNNLFTSSNPT 184
Query: 113 LV-RLDEISFKSISDADNELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFIQKFWDLL 171
+ E+ +S F EEV A+ + K+P PD F QK+W ++
Sbjct: 185 AIEETCEVVKGKLSHEHIVWCEKEFTEEEVLEAINQMHPVKAPGPDGLPALFFQKYWHIV 364
Query: 172 KDDFHRVL 179
+ +++
Sbjct: 365 GKEVQQMV 388
>AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (4%)
Length = 305
Score = 53.5 bits (127), Expect = 5e-08
Identities = 24/53 (45%), Positives = 34/53 (63%), Gaps = 2/53 (3%)
Frame = +1
Query: 136 FGMEEVKGAVWECGGDKS--PEPDEFNFKFIQKFWDLLKDDFHRVLEDFWNRG 186
F EEV+ AVW+C S P PD NF F++++W+L+K DF RV+ +F G
Sbjct: 121 FSEEEVRKAVWDCDSSHS*NPGPDGVNFTFVKEYWELIKVDFLRVVMEFHTNG 279
>TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 798
Score = 37.0 bits (84), Expect = 0.005
Identities = 21/74 (28%), Positives = 41/74 (55%)
Frame = -1
Query: 6 QKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTKYF 65
++L+++ K + G S+ ++ K + L + E ++KS+ W EGD+NT +F
Sbjct: 222 KELDDIQVKIDSIGYSDVLMDQEKAAQLNLESALNIEEVFWHEKSKVNWHCEGDRNTAFF 43
Query: 66 HSLINWRKRTNSIM 79
H + KRT+S++
Sbjct: 42 HRVAK-IKRTSSLI 4
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 35.4 bits (80), Expect = 0.014
Identities = 17/57 (29%), Positives = 28/57 (48%)
Frame = +3
Query: 130 ELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFIQKFWDLLKDDFHRVLEDFWNRG 186
+LL F EVK A++ K+P D +N F + W+++ D + DF+ G
Sbjct: 6 DLLCSEFTAVEVKNALFSMDSSKAPGIDGYNVHFFKCSWNIIGDSVIDAILDFFKTG 176
>TC82093
Length = 569
Score = 32.0 bits (71), Expect = 0.16
Identities = 19/48 (39%), Positives = 31/48 (64%), Gaps = 1/48 (2%)
Frame = -1
Query: 38 VLRLHE*LLYQKSRSKWLKEGDQNTKYFHSL-INWRKRTNSIMGLMID 84
+L +E L Y KS+ W+K G +NT +FH+ +N R +T+ GL++D
Sbjct: 476 LLSQNEMLWY*KSQ*NWVKYGSRNTWFFHNEDVN*RNQTS---GLIVD 342
>TC88690 weakly similar to GP|15724162|gb|AAL06473.1 AT3g59090/F17J16_140
{Arabidopsis thaliana}, partial (14%)
Length = 793
Score = 32.0 bits (71), Expect = 0.16
Identities = 19/78 (24%), Positives = 39/78 (49%)
Frame = -3
Query: 6 QKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTKYF 65
Q+LN + + S++ ++ +W+ + H * L ++SR KW K+ +
Sbjct: 290 QRLNSCNGSKNRVNNSKDNDDSEEWIRETVAPTESRHG*RLLKESRRKWKKKKE------ 129
Query: 66 HSLINWRKRTNSIMGLMI 83
L+N ++R SI+ ++I
Sbjct: 128 -VLLNSQRRERSILYILI 78
>BQ138029 weakly similar to GP|1764098|gb|A putative permease {Uromyces
fabae}, partial (10%)
Length = 656
Score = 28.9 bits (63), Expect = 1.3
Identities = 10/24 (41%), Positives = 18/24 (74%)
Frame = -2
Query: 67 SLINWRKRTNSIMGLMIDGNWVED 90
+++ R RTN ++GL++DG +ED
Sbjct: 130 TMVAGRVRTNGVLGLLVDGRHLED 59
>TC78468 similar to GP|21594872|gb|AAM66051.1 unknown {Arabidopsis
thaliana}, partial (78%)
Length = 1249
Score = 27.3 bits (59), Expect = 3.9
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -3
Query: 4 VVQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLH 42
V +K +E + EE EG EEN E +WL EF +H
Sbjct: 263 VEKKKSESENGEETEG---EENEEPEWLGFEFTDYATIH 156
>BG451876 weakly similar to GP|15144511|gb| unknown {Lycopersicon
esculentum}, partial (7%)
Length = 671
Score = 26.6 bits (57), Expect = 6.6
Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
Frame = +2
Query: 38 VLRLHE*LLYQKSRSKWLKEGDQ--NTKYFHSLINW 71
+LRL L +Q++ S W K + T YF L+ W
Sbjct: 497 LLRLSRILFFQRTDSSWKKSSSRYVMTSYFQPLLLW 604
>TC79820 similar to GP|21537325|gb|AAM61666.1 unknown {Arabidopsis
thaliana}, partial (89%)
Length = 1225
Score = 26.6 bits (57), Expect = 6.6
Identities = 18/54 (33%), Positives = 23/54 (42%)
Frame = -3
Query: 92 QAIKTEVQNYFEQCFTNSLGTLVRLDEISFKSISDADNELLTGRFGMEEVKGAV 145
Q + NY + NSL + +EI K NE G G EEVK +V
Sbjct: 389 QLANQQKTNYADNYPMNSLFNVTGCNEIPGKWYCPGGNEGEVGESGREEVKNSV 228
>TC93240 weakly similar to GP|14335170|gb|AAK59865.1 AT5g51070/K3K7_27
{Arabidopsis thaliana}, partial (8%)
Length = 700
Score = 26.6 bits (57), Expect = 6.6
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 8 LNELDRKEEEEGLSEEENNERKWLLDE 34
++E+ +EEEE EEE E ++L DE
Sbjct: 263 VSEVKEEEEEEEEEEEEEEEERFL*DE 183
>BI264438 similar to GP|21427467|gb actin-related protein 6 {Arabidopsis
thaliana}, partial (43%)
Length = 695
Score = 26.2 bits (56), Expect = 8.6
Identities = 13/28 (46%), Positives = 21/28 (74%)
Frame = -1
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERK 29
+E+V+++ L+ +E EEGLSEEE R+
Sbjct: 251 DELVERV--LNGRESEEGLSEEERGLRR 174
>TC77667 similar to PIR|T48625|T48625 pescadillo-like protein - Arabidopsis
thaliana, partial (60%)
Length = 1894
Score = 26.2 bits (56), Expect = 8.6
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 154 PEPDEFNFKFIQKFWDLLKDDFHRVL 179
PE F+F+ ++K W +LK + RVL
Sbjct: 1129 PERKSFHFQVLKKIWKILKIFWLRVL 1206
>TC89561 similar to GP|9759196|dbj|BAB09733.1 GTPase activator protein of
Rab-like small GTPases-like protein {Arabidopsis
thaliana}, partial (12%)
Length = 769
Score = 26.2 bits (56), Expect = 8.6
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -3
Query: 9 NELDRKEEEEGLSEEENNERKWLLDEF 35
+E + EEEE ERKW+ DEF
Sbjct: 650 DEQEAGEEEEEEVTTNKTERKWIHDEF 570
>TC83218
Length = 1170
Score = 26.2 bits (56), Expect = 8.6
Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -1
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWLLDE-FWT 37
N KL+ DR+ + +GL + E NE++ ++E WT
Sbjct: 879 NPETAKLHAQDRRRDVKGLGD*ERNEKR*SVNEVLWT 769
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.139 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,969,043
Number of Sequences: 36976
Number of extensions: 96753
Number of successful extensions: 557
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 538
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 551
length of query: 195
length of database: 9,014,727
effective HSP length: 91
effective length of query: 104
effective length of database: 5,649,911
effective search space: 587590744
effective search space used: 587590744
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0023.13