BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.1
(416 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM814840 weakly similar to GP|15128241|db helicase-like protein ... 144 6e-35
CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292... 95 4e-20
TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein... 75 6e-14
BI271341 63 2e-10
CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis ... 58 6e-09
AJ497367 similar to GP|14140286|gb putative helicase {Oryza sati... 57 2e-08
BE941280 weakly similar to GP|20197614|g unknown protein {Arabid... 55 4e-08
TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG282... 44 2e-04
TC80146 38 0.006
TC87692 similar to GP|21537190|gb|AAM61531.1 putative SKP1-like ... 29 3.8
TC78836 weakly similar to PIR|T47934|T47934 hypothetical protein... 28 5.0
TC87693 similar to GP|20197211|gb|AAC28530.2 E3 ubiquitin ligase... 28 5.0
TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1 ... 28 6.5
BE248373 weakly similar to GP|21629385|gb| L3223.3 {Leishmania m... 28 6.5
BG645082 similar to GP|20466073|gb At5g04300/At5g04300 {Arabidop... 28 6.5
BG447912 28 8.5
CA989308 similar to GP|21536872|gb unknown {Arabidopsis thaliana... 28 8.5
BM815360 similar to PIR|T45813|T458 hypothetical protein F28O9.2... 28 8.5
TC82875 similar to GP|15131529|emb|CAC48384. ethylene receptor {... 28 8.5
>BM814840 weakly similar to GP|15128241|db helicase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (6%)
Length = 733
Score = 144 bits (363), Expect = 6e-35
Identities = 70/123 (56%), Positives = 92/123 (73%)
Frame = +3
Query: 293 TTLMVIALTPYIIVATALSGTKSG*SFYIPRLSLTPSDTDLPFKFSRRQFPIIMCFVMTI 352
T L++++L +I A + GT +G YIPR++L PS ++ F R QFP+++ F MTI
Sbjct: 33 TRLIIVSLGKNVICARVIGGTHAGEVSYIPRMNLIPSGANVSITFERCQFPLVLSFAMTI 212
Query: 353 NKSEGQSLSHVGLYLPKPVFTHGQLYVALSRVKSRKGLKIFIVDDRGVVSNCTRNVVYEE 412
NKS+GQ+L+ VGLYLP+PVFTHGQLYVA+SRVKSR GLKI I D+ G S+ T NVVY+E
Sbjct: 213 NKSQGQTLTSVGLYLPRPVFTHGQLYVAVSRVKSRSGLKILITDENGSPSSSTVNVVYQE 392
Query: 413 VFQ 415
VFQ
Sbjct: 393 VFQ 401
>CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292.1
[imported] - Arabidopsis thaliana, partial (4%)
Length = 572
Score = 95.1 bits (235), Expect = 4e-20
Identities = 47/69 (68%), Positives = 53/69 (76%)
Frame = -2
Query: 348 FVMTINKSEGQSLSHVGLYLPKPVFTHGQLYVALSRVKSRKGLKIFIVDDRGVVSNCTRN 407
F MTINKS+GQSL H+G+YLP VF+HGQLYVALSRV SR+GLKI I +D G T N
Sbjct: 358 FAMTINKSQGQSLKHIGVYLPSSVFSHGQLYVALSRVTSREGLKILISNDDGEDDCVTSN 179
Query: 408 VVYEEVFQN 416
VVY EVF N
Sbjct: 178 VVYREVFHN 152
>TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein T24M8.10 -
Arabidopsis thaliana, partial (11%)
Length = 701
Score = 74.7 bits (182), Expect = 6e-14
Identities = 43/126 (34%), Positives = 68/126 (53%), Gaps = 1/126 (0%)
Frame = +3
Query: 158 NDDDSNIEIPSDLLVRESDNPILELVDFAYPEIVANLENHAYFEQRALLAHTLESVEEVN 217
ND + ++ P +PI +V YP +V+ N + + RA+L T E V+++N
Sbjct: 354 NDSYAEVDTPPG-------DPIDAIVQSTYPNLVSQYNNEQFLQSRAILTSTDEVVDQIN 512
Query: 218 NFMLSMIPGEETKYLSYDTPCRSDEDSDIDA-*WFTSEFLNDVKCSGIPNHRIVLKVEVP 276
+++L +IPGEE S E +D+ A EFL +K S +PNH++ LKV P
Sbjct: 513 DYVLKLIPGEERVIYS----ANRSEVNDVQAFDAIPPEFLQSLKTSDLPNHKLTLKVGTP 680
Query: 277 IMLIRN 282
IML+R+
Sbjct: 681 IMLLRD 698
>BI271341
Length = 468
Score = 62.8 bits (151), Expect = 2e-10
Identities = 33/101 (32%), Positives = 58/101 (56%)
Frame = +1
Query: 87 FRVPIPYIPLGSIATTFSSAVNSTYLWKYCKVIKLTINMRLQHAASSSSSMEIKELADWL 146
FR +P IP GS + + +NS+ + +C+V++L NM LQ SS+ E ++ + +
Sbjct: 1 FR*ILPVIPRGSRSDIIHATINSSCI*DHCQVVRLKKNMWLQQNGQSSNDPEFEQFSK*I 180
Query: 147 LQVGDEIVKPINDDDSNIEIPSDLLVRESDNPILELVDFAY 187
L+VGD + ND ++I+IP +LL+ D+ + +V Y
Sbjct: 181 LKVGDGKIYEPNDSYADIDIPPELLISNYDDSLQTIVQSTY 303
>CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis thaliana},
partial (3%)
Length = 748
Score = 58.2 bits (139), Expect = 6e-09
Identities = 29/51 (56%), Positives = 38/51 (73%)
Frame = -1
Query: 366 YLPKPVFTHGQLYVALSRVKSRKGLKIFIVDDRGVVSNCTRNVVYEEVFQN 416
+ + VF+HGQLYVA+SRV SR GLKI ++D+ G + T NVVY +VFQN
Sbjct: 289 FYDREVFSHGQLYVAISRVSSRNGLKILMIDENGDCIDNTTNVVY-KVFQN 140
>AJ497367 similar to GP|14140286|gb putative helicase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 543
Score = 56.6 bits (135), Expect = 2e-08
Identities = 27/45 (60%), Positives = 35/45 (77%)
Frame = +1
Query: 372 FTHGQLYVALSRVKSRKGLKIFIVDDRGVVSNCTRNVVYEEVFQN 416
F++G+LYVA+SRV SRKGLKI + + G N T NVVY+EVF+N
Sbjct: 19 FSNGKLYVAVSRVTSRKGLKILLAHEDGNCMNTTSNVVYKEVFRN 153
>BE941280 weakly similar to GP|20197614|g unknown protein {Arabidopsis
thaliana}, partial (8%)
Length = 403
Score = 55.5 bits (132), Expect = 4e-08
Identities = 26/46 (56%), Positives = 30/46 (64%)
Frame = -1
Query: 2 SLLLPGGRTAHSRFSIPISIHEISTCNVRQGSPKAEMLQKVSLIIW 47
+LL+PGGRTAHSRF IP I E S C V P A ++ K LIIW
Sbjct: 295 ALLMPGGRTAHSRFGIPFIIDETSMCGVTPNIPLASLVIKAKLIIW 158
>TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG28292.1
[imported] - Arabidopsis thaliana, partial (1%)
Length = 1009
Score = 43.5 bits (101), Expect = 2e-04
Identities = 20/30 (66%), Positives = 25/30 (82%)
Frame = +2
Query: 359 SLSHVGLYLPKPVFTHGQLYVALSRVKSRK 388
S++ G+YLP+P+F HG LYVALSRV SRK
Sbjct: 68 SMATKGMYLPQPIF*HG*LYVALSRVTSRK 157
>TC80146
Length = 476
Score = 38.1 bits (87), Expect = 0.006
Identities = 15/31 (48%), Positives = 24/31 (77%)
Frame = -3
Query: 348 FVMTINKSEGQSLSHVGLYLPKPVFTHGQLY 378
+ TINKS QSLS++ +YL +P+F+H ++Y
Sbjct: 459 YFKTINKSR*QSLSYMKIYLSRPIFSHEEMY 367
>TC87692 similar to GP|21537190|gb|AAM61531.1 putative SKP1-like protein
{Arabidopsis thaliana}, partial (50%)
Length = 1539
Score = 28.9 bits (63), Expect = 3.8
Identities = 26/99 (26%), Positives = 47/99 (47%), Gaps = 2/99 (2%)
Frame = +3
Query: 119 IKLTINMRLQHAASSSSSMEIKELADWLLQVGDEIVKPINDDD--SNIEIPSDLLVRESD 176
+K+ NM L A ++ S++ + L D + I++ + ++ S +P DL E
Sbjct: 555 VKIDTNM-LCELACAAHSLQFRPLIDLTSRALARIIEKRSPEEIRSIFRVPDDLTEEEKL 731
Query: 177 NPILELVDFAYPEIVANLENHAYFEQRALLAHTLESVEE 215
P+L + D P I L N Y ++R L ES+++
Sbjct: 732 EPLLNITD--DPSI--RLLNRLYAKKRRELLKERESIKK 836
>TC78836 weakly similar to PIR|T47934|T47934 hypothetical protein F2A19.20 -
Arabidopsis thaliana, partial (7%)
Length = 1075
Score = 28.5 bits (62), Expect = 5.0
Identities = 26/69 (37%), Positives = 34/69 (48%)
Frame = +2
Query: 13 SRFSIPISIHEISTCNVRQGSPKAEMLQKVSLIIWTIHSISTVILQPPL*TFNSFIGP*N 72
SRFS+P +I IST R SPK + ++ + SI V+L L T F G *
Sbjct: 128 SRFSLPNAIIPISTKRSRMLSPKRD-------VLISDFSILKVLLN*TL-TAIEFYGG*R 283
Query: 73 CIPYENKSL 81
C+ NK L
Sbjct: 284 CLRRFNKLL 310
>TC87693 similar to GP|20197211|gb|AAC28530.2 E3 ubiquitin ligase SCF
complex subunit SKP1/ASK1-related {Arabidopsis
thaliana}, partial (72%)
Length = 831
Score = 28.5 bits (62), Expect = 5.0
Identities = 27/99 (27%), Positives = 46/99 (46%), Gaps = 2/99 (2%)
Frame = +1
Query: 119 IKLTINMRLQHAASSSSSMEIKELADWLLQVGDEIVKPINDDD--SNIEIPSDLLVRESD 176
+K+ NM L A ++ S++ + L D + I++ + ++ S +P DL E
Sbjct: 520 VKIDTNM-LCELACAAHSLQFRPLIDLTSRALARIIEKRSPEEIRSIFRVPDDLTEEEKL 696
Query: 177 NPILELVDFAYPEIVANLENHAYFEQRALLAHTLESVEE 215
P+L + D P I L N Y ++R L ES+ E
Sbjct: 697 EPLLNITD--DPSI--TLLNRLYAKKRRELLKERESI*E 801
>TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1
{Arabidopsis thaliana}, partial (47%)
Length = 1538
Score = 28.1 bits (61), Expect = 6.5
Identities = 17/47 (36%), Positives = 24/47 (50%)
Frame = -1
Query: 72 NCIPYENKSLKFFSRFRVPIPYIPLGSIATTFSSAVNSTYLWKYCKV 118
+ I + +SL+FF RFR+ P I I T + S +LWK V
Sbjct: 380 SAILFITQSLQFFIRFRLVTPRIVAVEIGTVITV---SEFLWKMVSV 249
>BE248373 weakly similar to GP|21629385|gb| L3223.3 {Leishmania major},
partial (8%)
Length = 277
Score = 28.1 bits (61), Expect = 6.5
Identities = 9/27 (33%), Positives = 17/27 (62%)
Frame = -2
Query: 83 FFSRFRVPIPYIPLGSIATTFSSAVNS 109
F + F +P P+ P+ S+ + S ++NS
Sbjct: 261 FHNPFSIPFPFFPISSVDVSISRSINS 181
>BG645082 similar to GP|20466073|gb At5g04300/At5g04300 {Arabidopsis
thaliana}, partial (41%)
Length = 782
Score = 28.1 bits (61), Expect = 6.5
Identities = 15/58 (25%), Positives = 26/58 (43%)
Frame = -1
Query: 76 YENKSLKFFSRFRVPIPYIPLGSIATTFSSAVNSTYLWKYCKVIKLTINMRLQHAASS 133
Y+ +S+ + R PIP P I + S + + C + LT+N R+ + S
Sbjct: 329 YKERSIYHYIPLRPPIPTTPFFIICIHALCHLFSICIGRSCVTVTLTVNSRISTTSDS 156
>BG447912
Length = 645
Score = 27.7 bits (60), Expect = 8.5
Identities = 16/42 (38%), Positives = 21/42 (49%)
Frame = -3
Query: 225 PGEETKYLSYDTPCRSDEDSDIDA*WFTSEFLNDVKCSGIPN 266
PG E ++ D C D+DA SEFL D+K + PN
Sbjct: 439 PGREASRINLDGRC-----PDVDAAAVESEFLTDLKTNFEPN 329
>CA989308 similar to GP|21536872|gb unknown {Arabidopsis thaliana}, partial
(41%)
Length = 583
Score = 27.7 bits (60), Expect = 8.5
Identities = 14/42 (33%), Positives = 24/42 (56%), Gaps = 5/42 (11%)
Frame = +1
Query: 334 PFKFSRR-----QFPIIMCFVMTINKSEGQSLSHVGLYLPKP 370
PF+ S + QFP+++C+V S G+ H+ L+ P+P
Sbjct: 37 PFQISTQRC*LYQFPLVLCWVSITFSSHGE--IHIYLFQPQP 156
>BM815360 similar to PIR|T45813|T458 hypothetical protein F28O9.200 -
Arabidopsis thaliana, partial (1%)
Length = 742
Score = 27.7 bits (60), Expect = 8.5
Identities = 14/37 (37%), Positives = 20/37 (53%)
Frame = +1
Query: 88 RVPIPYIPLGSIATTFSSAVNSTYLWKYCKVIKLTIN 124
R+ P + LG + F N YL+KYC ++ L IN
Sbjct: 529 RLHNPLLQLGYLVVFF----NFNYLYKYCMILFLAIN 627
>TC82875 similar to GP|15131529|emb|CAC48384. ethylene receptor {Fragaria x
ananassa}, partial (39%)
Length = 1118
Score = 27.7 bits (60), Expect = 8.5
Identities = 13/44 (29%), Positives = 22/44 (49%)
Frame = +3
Query: 366 YLPKPVFTHGQLYVALSRVKSRKGLKIFIVDDRGVVSNCTRNVV 409
Y PK HG ++ GLK+ ++DD GV + T+ ++
Sbjct: 489 YKPKASVNHGSTNIS--------GLKVLVMDDNGVSRSATKGLL 596
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.141 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,101,238
Number of Sequences: 36976
Number of extensions: 181463
Number of successful extensions: 1008
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 1004
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1007
length of query: 416
length of database: 9,014,727
effective HSP length: 99
effective length of query: 317
effective length of database: 5,354,103
effective search space: 1697250651
effective search space used: 1697250651
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0023.1