BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0022.20
(96 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77422 similar to PIR|T04704|T04704 hypothetical protein F4B14.... 32 0.031
TC85442 similar to GP|13543783|gb|AAH06040.1 Unknown (protein fo... 32 0.053
TC80831 similar to GP|18542939|gb|AAK00428.2 Unknown protein {Or... 31 0.090
TC86288 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max},... 31 0.090
TC90786 similar to PIR|B84476|B84476 probable TPR repeat nuclear... 30 0.15
TC88253 weakly similar to GP|15810597|gb|AAL07186.1 unknown prot... 30 0.20
TC83333 similar to GP|15028195|gb|AAK76594.1 unknown protein {Ar... 30 0.20
BQ151924 homologue to GP|7340791|emb ribosomal protein L23 {Spin... 29 0.26
BQ141736 similar to PIR|T06291|T062 extensin homolog T9E8.80 - A... 29 0.26
BQ147823 similar to GP|18146720|dbj ribosomal protein S2 {Arabid... 29 0.26
TC85487 homologue to SP|P49688|RS2_ARATH 40S ribosomal protein S... 29 0.26
TC85490 similar to GP|18146720|dbj|BAB82426. ribosomal protein S... 29 0.34
BQ150836 28 0.45
BQ151366 similar to GP|20146300|dbj hypothetical protein~similar... 28 0.59
BQ137241 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 28 0.59
TC84066 similar to GP|23498475|emb|CAD50426. ribonuclease putat... 28 0.59
BQ144398 similar to GP|13122418|dbj putative glycin-rich protein... 28 0.77
AW688848 homologue to PIR|T46027|T4 hypothetical protein T10K17.... 28 0.77
TC92089 27 1.0
TC77396 similar to GP|23175890|gb|AAN14305.1 CG1658-PC {Drosophi... 26 2.2
>TC77422 similar to PIR|T04704|T04704 hypothetical protein F4B14.210 -
Arabidopsis thaliana, partial (9%)
Length = 1448
Score = 32.3 bits (72), Expect = 0.031
Identities = 18/48 (37%), Positives = 29/48 (59%), Gaps = 3/48 (6%)
Frame = +1
Query: 3 RRRTEEEDSKKKKKRGR---GGRRRTQPYHESKKKEKMRLKKRNREVL 47
RRRT + +K+ ++RGR G RRR + E KKK ++++ +VL
Sbjct: 226 RRRTRKPPTKRPRRRGRRGGGRRRRRRKKGEGKKKRN*KIQQPLMQVL 369
>TC85442 similar to GP|13543783|gb|AAH06040.1 Unknown (protein for MGC:7642)
{Mus musculus}, partial (62%)
Length = 585
Score = 31.6 bits (70), Expect = 0.053
Identities = 12/43 (27%), Positives = 29/43 (66%)
Frame = +1
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNRE 45
R++++ + +++KKK+ + +RR + + K+++ R KKR R+
Sbjct: 70 RKKSQRKKNQRKKKKRKKKQRRKKRRKKGKRRKNQRKKKRKRK 198
Score = 30.0 bits (66), Expect = 0.15
Identities = 15/46 (32%), Positives = 28/46 (60%)
Frame = +1
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLR 48
R++ + + +++KKR + G+RR + + K+K K R KK R+ R
Sbjct: 100 RKKKKRKKKQRRKKRRKKGKRR-KNQRKKKRKRKRRKKKGKRKKRR 234
>TC80831 similar to GP|18542939|gb|AAK00428.2 Unknown protein {Oryza
sativa}, partial (7%)
Length = 1039
Score = 30.8 bits (68), Expect = 0.090
Identities = 14/40 (35%), Positives = 29/40 (72%), Gaps = 1/40 (2%)
Frame = +2
Query: 7 EEEDSKKKKKRGRGGRRRTQPYHES-KKKEKMRLKKRNRE 45
EEE+ +K+++ R RRRT+ + ++KE+++ K+++RE
Sbjct: 134 EEEEKEKREEEERKERRRTKEREKKLRRKERLKGKEKDRE 253
>TC86288 similar to GP|2407790|gb|AAB70660.1| grr1 {Glycine max}, partial
(84%)
Length = 2900
Score = 30.8 bits (68), Expect = 0.090
Identities = 17/40 (42%), Positives = 24/40 (59%)
Frame = -2
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKR 42
R + EE +KKKKRGRG +PY +KK++ R K +
Sbjct: 121 REKEEELLREKKKKRGRG----REPYEIGQKKKREREKMK 14
>TC90786 similar to PIR|B84476|B84476 probable TPR repeat nuclear
phosphoprotein [imported] - Arabidopsis thaliana,
partial (26%)
Length = 1563
Score = 30.0 bits (66), Expect = 0.15
Identities = 16/40 (40%), Positives = 26/40 (65%), Gaps = 6/40 (15%)
Frame = +3
Query: 3 RRRTEEEDS-KKKKKRGRGGRRRTQPYH-----ESKKKEK 36
R R+++ED +KKR +GG+RR + H ++KKK+K
Sbjct: 864 RERSDDEDGGTAEKKRRKGGKRRKKDKHSKSHNDNKKKQK 983
>TC88253 weakly similar to GP|15810597|gb|AAL07186.1 unknown protein
{Arabidopsis thaliana}, partial (51%)
Length = 1122
Score = 29.6 bits (65), Expect = 0.20
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = +1
Query: 13 KKKKRGRGGRRRTQPYHESKKKEKMRLKKRNRE 45
++KKR + R++ + SKKK K ++KK+ +E
Sbjct: 697 RRKKRRKKRRKKKRKRKRSKKKRKKKVKKKTKE 795
Score = 28.5 bits (62), Expect = 0.45
Identities = 15/44 (34%), Positives = 27/44 (61%)
Frame = +1
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREV 46
R++ ++ KKK+KR R ++R + + K KEKM +K ++ V
Sbjct: 700 RKKRRKKRRKKKRKRKRSKKKRKKKV-KKKTKEKMIIKLKSNVV 828
>TC83333 similar to GP|15028195|gb|AAK76594.1 unknown protein {Arabidopsis
thaliana}, partial (15%)
Length = 443
Score = 29.6 bits (65), Expect = 0.20
Identities = 20/71 (28%), Positives = 32/71 (44%), Gaps = 5/71 (7%)
Frame = +3
Query: 29 HESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNI-----YITTYPFKITQVMKKKVKP 83
++ KKK+K ++ R +L LH ++T L ++ +PF + P
Sbjct: 48 NQPKKKKKWQMY*RVAVLLYSHSSLLHCTTTTLSRTITNYSFLPKFPFLSLSSSSRPKTP 227
Query: 84 SLASGACTLSS 94
S S CTLSS
Sbjct: 228 SSFSSFCTLSS 260
>BQ151924 homologue to GP|7340791|emb ribosomal protein L23 {Spinacia
oleracea}, partial (76%)
Length = 701
Score = 29.3 bits (64), Expect = 0.26
Identities = 14/23 (60%), Positives = 14/23 (60%)
Frame = +3
Query: 9 EDSKKKKKRGRGGRRRTQPYHES 31
E SKKKKK RGG PY ES
Sbjct: 558 ELSKKKKKNSRGGPXPNSPYXES 626
>BQ141736 similar to PIR|T06291|T062 extensin homolog T9E8.80 - Arabidopsis
thaliana, partial (4%)
Length = 1099
Score = 29.3 bits (64), Expect = 0.26
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +2
Query: 9 EDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNREVLR 48
E+ KKK+KRGR RR + +++ R + RE+ R
Sbjct: 644 EERKKKRKRGRRKRRSDENTKRGREERT*RRARHEREIRR 763
>BQ147823 similar to GP|18146720|dbj ribosomal protein S2 {Arabidopsis
thaliana}, partial (31%)
Length = 695
Score = 29.3 bits (64), Expect = 0.26
Identities = 26/89 (29%), Positives = 39/89 (43%), Gaps = 4/89 (4%)
Frame = +3
Query: 12 KKKKK----RGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNIYIT 67
KKKK RGRGGRR E ++K + + K R V +L+ IY+
Sbjct: 402 KKKKSGRGDRGRGGRRXAGGRREEEEK-WVPVTKLGRLV-------KEXKIRSLEQIYLH 557
Query: 68 TYPFKITQVMKKKVKPSLASGACTLSSLQ 96
+ P K Q++ V P+L + +Q
Sbjct: 558 SLPIKEHQIIDTLVGPTLKDXVMKIMPVQ 644
>TC85487 homologue to SP|P49688|RS2_ARATH 40S ribosomal protein S2.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (88%)
Length = 1114
Score = 29.3 bits (64), Expect = 0.26
Identities = 23/80 (28%), Positives = 36/80 (44%)
Frame = +1
Query: 17 RGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNIYITTYPFKITQV 76
RGRGGRRR E ++K + + K R V +L+ IY+ + P K Q+
Sbjct: 163 RGRGGRRRAGGRREEEEK-WVPVTKLGRLV-------KEGKIRSLEQIYLHSLPIKEHQI 318
Query: 77 MKKKVKPSLASGACTLSSLQ 96
+ V P+L + +Q
Sbjct: 319 IDTLVGPTLKDEVMKIMPVQ 378
>TC85490 similar to GP|18146720|dbj|BAB82426. ribosomal protein S2
{Arabidopsis thaliana}, partial (89%)
Length = 1252
Score = 28.9 bits (63), Expect = 0.34
Identities = 23/80 (28%), Positives = 36/80 (44%)
Frame = +1
Query: 17 RGRGGRRRTQPYHESKKKEKMRLKKRNREVLRFIEQFLHSSSTNLQNIYITTYPFKITQV 76
RGRGGRRR E ++K + + K R V +L+ IY+ + P K Q+
Sbjct: 142 RGRGGRRRGAGRREEEEK-WVPVTKLGRLV-------KEGKIRSLEQIYLHSLPIKEHQI 297
Query: 77 MKKKVKPSLASGACTLSSLQ 96
+ V P+L + +Q
Sbjct: 298 IDTLVGPTLKDEVMKIMPVQ 357
>BQ150836
Length = 628
Score = 28.5 bits (62), Expect = 0.45
Identities = 13/41 (31%), Positives = 27/41 (65%)
Frame = -1
Query: 35 EKMRLKKRNREVLRFIEQFLHSSSTNLQNIYITTYPFKITQ 75
E+ + K+++ V+ F+ L ++ TN+ + IT YPF+++Q
Sbjct: 124 ERNQKNKKDKMVVFFLFYNLLTNYTNILALAITHYPFRLSQ 2
>BQ151366 similar to GP|20146300|dbj hypothetical protein~similar to Oryza
sativa OSJNBa0058E19.20, partial (9%)
Length = 1137
Score = 28.1 bits (61), Expect = 0.59
Identities = 14/43 (32%), Positives = 23/43 (52%)
Frame = +1
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNRE 45
RRR +E +++G+G R T P +++KEK +R E
Sbjct: 277 RRRKTKERRTSPRQKGQGKRAATTPPRPTRRKEKDGRNERPTE 405
>BQ137241 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (8%)
Length = 1176
Score = 28.1 bits (61), Expect = 0.59
Identities = 13/26 (50%), Positives = 20/26 (76%), Gaps = 1/26 (3%)
Frame = +3
Query: 2 GRRRTEEEDSKKKKKRG-RGGRRRTQ 26
GRR EEE+S + +K G RGGR++++
Sbjct: 441 GRREKEEEESARGRKGGGRGGRKQSR 518
>TC84066 similar to GP|23498475|emb|CAD50426. ribonuclease putative
{Plasmodium falciparum 3D7}, partial (0%)
Length = 481
Score = 28.1 bits (61), Expect = 0.59
Identities = 14/42 (33%), Positives = 24/42 (56%)
Frame = +3
Query: 3 RRRTEEEDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRNR 44
++R E K KKK+G+ R+ + + K+KEK R + + R
Sbjct: 339 KKRKRERRRKGKKKKGKR**RKRRRIGKEKEKEKERTRMQGR 464
>BQ144398 similar to GP|13122418|dbj putative glycin-rich protein {Oryza
sativa (japonica cultivar-group)}, partial (10%)
Length = 1322
Score = 27.7 bits (60), Expect = 0.77
Identities = 12/35 (34%), Positives = 20/35 (56%)
Frame = +2
Query: 9 EDSKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRN 43
+ + +K + GG+R T+ + KKK K R K R+
Sbjct: 428 QHNNRKTTKKEGGKRETKKTAQRKKKPKARKKNRS 532
>AW688848 homologue to PIR|T46027|T4 hypothetical protein T10K17.260 -
Arabidopsis thaliana, partial (10%)
Length = 661
Score = 27.7 bits (60), Expect = 0.77
Identities = 12/40 (30%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
Frame = +1
Query: 7 EEEDSKKKKKRGRGGRRRTQPYHES-KKKEKMRLKKRNRE 45
EEE+ +K+++ R R+R + + ++KE+++ K +N+E
Sbjct: 259 EEEEKEKREEEERKERKRAKEREKKLRRKERLKGKDKNKE 378
>TC92089
Length = 696
Score = 27.3 bits (59), Expect = 1.0
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 11 SKKKKKRGRGGRRRTQPYHESKKKEKMRLKKRN 43
+K KK GG+R+ PY+ K E ++K N
Sbjct: 86 AKTAKKATGGGKRKLSPYNAFMKSELPKVKAEN 184
>TC77396 similar to GP|23175890|gb|AAN14305.1 CG1658-PC {Drosophila
melanogaster}, partial (2%)
Length = 790
Score = 26.2 bits (56), Expect = 2.2
Identities = 12/21 (57%), Positives = 12/21 (57%)
Frame = +1
Query: 11 SKKKKKRGRGGRRRTQPYHES 31
SKKKKK RGG PY S
Sbjct: 697 SKKKKKNSRGGPAPNXPYSXS 759
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.129 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,793,242
Number of Sequences: 36976
Number of extensions: 34093
Number of successful extensions: 843
Number of sequences better than 10.0: 152
Number of HSP's better than 10.0 without gapping: 774
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 820
length of query: 96
length of database: 9,014,727
effective HSP length: 72
effective length of query: 24
effective length of database: 6,352,455
effective search space: 152458920
effective search space used: 152458920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0022.20