BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0021.5
(210 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ144352 similar to GP|3043568|dbj KIAA0522 protein {Homo sapien... 37 0.007
TC93017 similar to GP|9293960|dbj|BAB01863.1 ubiquitin conjugati... 35 0.027
TC88260 homologue to GP|15215696|gb|AAK91394.1 AT3g16950/K14A17_... 33 0.061
BQ144307 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 33 0.061
TC90393 similar to GP|15293299|gb|AAK93760.1 unknown protein {Ar... 33 0.10
TC88061 homologue to SP|Q43117|KPYA_RICCO Pyruvate kinase isozym... 32 0.14
BQ141909 weakly similar to GP|14253159|emb VMP3 protein {Volvox ... 32 0.18
BQ751524 homologue to GP|2911462|gb| serine/threonine protein ki... 32 0.18
BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 32 0.23
BQ750926 similar to PIR|T51049|T510 related to nucleolar phospho... 32 0.23
BQ143960 weakly similar to SP|P21997|SSGP_ Sulfated surface glyc... 31 0.30
TC81708 similar to GP|12083536|gb|AAG48845.1 hypothetical protei... 31 0.30
BF648665 weakly similar to GP|15294296|gb AT3g56820/T8M16_150 {A... 31 0.30
TC79235 weakly similar to GP|7297741|gb|AAF52992.1| CG17108 gene... 31 0.39
TC77892 similar to GP|14861486|gb|AAK73693.1 succinate dehydroge... 31 0.39
TC82658 homologue to GP|5917666|gb|AAD55980.1| extensin-like pro... 31 0.39
BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, pa... 31 0.39
BQ079360 homologue to SP|Q40358|PRP_ Repetitive proline-rich cel... 30 0.51
BQ144306 weakly similar to GP|23093252|gb| CG13731-PA {Drosophil... 30 0.51
AL369112 weakly similar to GP|13540393|gb| histone H1 {Pisum sat... 30 0.51
>BQ144352 similar to GP|3043568|dbj KIAA0522 protein {Homo sapiens}, partial
(1%)
Length = 818
Score = 36.6 bits (83), Expect = 0.007
Identities = 35/149 (23%), Positives = 56/149 (37%), Gaps = 2/149 (1%)
Frame = +1
Query: 48 PPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCTPL 107
PPR+ P PL+ P PPC P P ++ +P S + NQ H
Sbjct: 130 PPRSFSRPLPLYIPLTNHPPC-----SPFPHPPQTTHIHNPASPYVFLVHPNQTH----- 279
Query: 108 TVRNKTPQEIFRFAALRRRASALQLAISPSFGLRWPRNHHHHALHGERNKTLYSFSSPAT 167
+ TP LQL SP+ R P HH ++ Y + + T
Sbjct: 280 -LTRNTPH-------------TLQLYPSPTNSTRLPNTTPHHTPQPHT*QSPYPYLTTIT 417
Query: 168 LPPLAAVLRHL--LQPISPEKSAELVLKI 194
L + + ++ I P KS +++++
Sbjct: 418 LIQQTPHQQDIAYIRTIIPIKSLPVMIRV 504
>TC93017 similar to GP|9293960|dbj|BAB01863.1 ubiquitin conjugating
protein-like {Arabidopsis thaliana}, partial (16%)
Length = 816
Score = 34.7 bits (78), Expect = 0.027
Identities = 11/16 (68%), Positives = 14/16 (86%)
Frame = +1
Query: 133 AISPSFGLRWPRNHHH 148
A++P FG+ WPRNHHH
Sbjct: 76 AVAPPFGIVWPRNHHH 123
Score = 28.1 bits (61), Expect = 2.5
Identities = 13/40 (32%), Positives = 17/40 (42%)
Frame = +1
Query: 96 IWRNQHHHCTPLTVRNKTPQEIFRFAALRRRASALQLAIS 135
+W HHH TPL K IFR ++ L +S
Sbjct: 100 VWPRNHHH*TPLFFLFKNHPSIFRLVEFQKSQPPPSLLLS 219
>TC88260 homologue to GP|15215696|gb|AAK91394.1 AT3g16950/K14A17_7
{Arabidopsis thaliana}, partial (80%)
Length = 2274
Score = 33.5 bits (75), Expect = 0.061
Identities = 25/69 (36%), Positives = 28/69 (40%), Gaps = 3/69 (4%)
Frame = +3
Query: 87 SPIS---GEPLTIWRNQHHHCTPLTVRNKTPQEIFRFAALRRRASALQLAISPSFGLRWP 143
SPIS P TI + HHC+ RF +LRR A A S R P
Sbjct: 60 SPISLSFSSPTTI--SGPHHCSLFNTTTTATSLTLRFCSLRREAFAF-----TSLSHRSP 218
Query: 144 RNHHHHALH 152
RNHH H
Sbjct: 219 RNHHLRLTH 245
>BQ144307 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (24%)
Length = 1059
Score = 33.5 bits (75), Expect = 0.061
Identities = 21/57 (36%), Positives = 28/57 (48%), Gaps = 1/57 (1%)
Frame = -1
Query: 42 ATRKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRY-LQSPISGEPLTIW 97
+T +PPPP P P P P PP H A PR+P+ Y L P + P +I+
Sbjct: 492 STPRPPPPSPHFSPPPPPAPSPP-PPLSRHPAHPNPRSPTSTYALPPPRTSPPESIY 325
>TC90393 similar to GP|15293299|gb|AAK93760.1 unknown protein {Arabidopsis
thaliana}, partial (14%)
Length = 754
Score = 32.7 bits (73), Expect = 0.10
Identities = 19/61 (31%), Positives = 32/61 (52%)
Frame = +2
Query: 109 VRNKTPQEIFRFAALRRRASALQLAISPSFGLRWPRNHHHHALHGERNKTLYSFSSPATL 168
++N + + F F++L +++ L+L F L R H+HH HG R +S AT+
Sbjct: 80 IKNTSNES*FSFSSLNQKSETLKLF----FALLLSRRHNHHHRHGSR------VNSVATI 229
Query: 169 P 169
P
Sbjct: 230 P 232
>TC88061 homologue to SP|Q43117|KPYA_RICCO Pyruvate kinase isozyme A
chloroplast precursor (EC 2.7.1.40). [Castor bean]
{Ricinus communis}, partial (84%)
Length = 1765
Score = 32.3 bits (72), Expect = 0.14
Identities = 23/70 (32%), Positives = 31/70 (43%)
Frame = +1
Query: 46 PPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCT 105
PP P+T P P P + LPP H A P P QSP + P R+ HH
Sbjct: 133 PPQPQTST-PTP---PTSHLPPSTSHSAASPLSAPQPPT*QSPTTTAP----RHHRHHHL 288
Query: 106 PLTVRNKTPQ 115
+ ++K+ Q
Sbjct: 289 QIQTQSKSTQ 318
>BQ141909 weakly similar to GP|14253159|emb VMP3 protein {Volvox carteri f.
nagariensis}, partial (13%)
Length = 1223
Score = 32.0 bits (71), Expect = 0.18
Identities = 20/50 (40%), Positives = 22/50 (44%), Gaps = 6/50 (12%)
Frame = -3
Query: 46 PPPPRTLLLPFPLFQPRAPLPPCL------LHEAMKPPRNPSRRYLQSPI 89
PPPP P PL P P PP L A PP PS R+ SP+
Sbjct: 306 PPPP---FFPLPLLPPPLPPPPLLPLPLSDPPSAPPPPSPPSPRFPLSPL 166
>BQ751524 homologue to GP|2911462|gb| serine/threonine protein kinase
{Colletotrichum trifolii}, partial (22%)
Length = 641
Score = 32.0 bits (71), Expect = 0.18
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +3
Query: 35 RDDTRKAATRKPPPPRTLLLPFPLFQPRAPLPPCLL 70
RD T +P PP TL + FPL P A L P +L
Sbjct: 333 RDSPYPNNTDRPSPPHTLKVRFPLVAPSASLAPYVL 440
>BQ144482 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (16%)
Length = 993
Score = 31.6 bits (70), Expect = 0.23
Identities = 27/75 (36%), Positives = 30/75 (40%), Gaps = 9/75 (12%)
Frame = +1
Query: 45 KPPPPRTLLLPF-----PLFQPRAPLPPCLLHEAMKPPRNPSR----RYLQSPISGEPLT 95
+PPPP P P F P AP PP PPR P+R R L P +G P
Sbjct: 253 RPPPPPPPPPPLSLSLAPAFAPPAPGPP----RPPPPPRPPARPRPARVLAGP-AGRP-R 414
Query: 96 IWRNQHHHCTPLTVR 110
WR TP R
Sbjct: 415 HWRASRRRVTPTPAR 459
Score = 28.5 bits (62), Expect = 2.0
Identities = 23/57 (40%), Positives = 25/57 (43%), Gaps = 11/57 (19%)
Frame = +2
Query: 38 TRKAATRKP------PPPRTLLLPFPLFQPRAPLPPCLLHEAM-----KPPRNPSRR 83
+R A T P PPP L LP P F P PLPP A PPR P+ R
Sbjct: 170 SRPAPTFSPPPPLLSPPPLPLSLPSPSFAP-LPLPPPPPPSASPWPRPSPPRPPAPR 337
Score = 26.9 bits (58), Expect = 5.7
Identities = 16/40 (40%), Positives = 17/40 (42%), Gaps = 3/40 (7%)
Frame = +1
Query: 44 RKPPPPRTLLLPFP---LFQPRAPLPPCLLHEAMKPPRNP 80
R P PR L P P + P PLPP L PP P
Sbjct: 160 RPPKSPRAHLFPPPSPSISAPPPPLPPLPLFRPPPPPPPP 279
>BQ750926 similar to PIR|T51049|T510 related to nucleolar phosphoprotein
[imported] - Neurospora crassa, partial (9%)
Length = 779
Score = 31.6 bits (70), Expect = 0.23
Identities = 26/90 (28%), Positives = 34/90 (36%), Gaps = 4/90 (4%)
Frame = -3
Query: 61 PRAPLPPCLLHEAMKPPRNPSRRYLQSP----ISGEPLTIWRNQHHHCTPLTVRNKTPQE 116
PR P + ++ +PPR LQSP + G T WR Q C +V E
Sbjct: 756 PREPWQALVHSKSCQPPRTCPLHRLQSPYLRRLCGYGRT-WRQQTTRCDQSSVMRWRITE 580
Query: 117 IFRFAALRRRASALQLAISPSFGLRWPRNH 146
+ R P+ LRWPR H
Sbjct: 579 LVR---------------QPALSLRWPRVH 535
>BQ143960 weakly similar to SP|P21997|SSGP_ Sulfated surface glycoprotein 185
(SSG 185). {Volvox carteri}, partial (13%)
Length = 1023
Score = 31.2 bits (69), Expect = 0.30
Identities = 25/83 (30%), Positives = 31/83 (37%), Gaps = 21/83 (25%)
Frame = +2
Query: 46 PPPPRTLLLPFPLFQPRAPLPPCL----LHEAMKPPR-----------------NPSRRY 84
PPPP + PFP P P PP + L + PP NP Y
Sbjct: 14 PPPPPS---PFPSSPPPPPSPPLISPPFLSHFLFPPHPFPFFFHLFFNPFFFIINPVSNY 184
Query: 85 LQSPISGEPLTIWRNQHHHCTPL 107
+PI P + H+H TPL
Sbjct: 185 FNTPIPPPPPPF--SPHYHHTPL 247
Score = 26.6 bits (57), Expect = 7.4
Identities = 13/23 (56%), Positives = 13/23 (56%), Gaps = 2/23 (8%)
Frame = +1
Query: 47 PPPRTLLLPFPL--FQPRAPLPP 67
PPPR PFPL P PLPP
Sbjct: 1 PPPRPPAPPFPLPLLPPPPPLPP 69
>TC81708 similar to GP|12083536|gb|AAG48845.1 hypothetical protein {Oryza
sativa}, partial (6%)
Length = 605
Score = 31.2 bits (69), Expect = 0.30
Identities = 19/46 (41%), Positives = 22/46 (47%), Gaps = 8/46 (17%)
Frame = -1
Query: 43 TRKPPPPRTLLLPFPLF-------QPRAPLPP-CLLHEAMKPPRNP 80
TRKPPPP LP P F P++P PP C PP +P
Sbjct: 224 TRKPPPP---FLPLPFFFLLLFFSSPQSPPPPFCFSPLFFLPPSSP 96
>BF648665 weakly similar to GP|15294296|gb AT3g56820/T8M16_150 {Arabidopsis
thaliana}, partial (48%)
Length = 647
Score = 31.2 bits (69), Expect = 0.30
Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 6/47 (12%)
Frame = +2
Query: 28 VVLTRCY------RDDTRKAATRKPPPPRTLLLPFPLFQPRAPLPPC 68
VV T+C+ R+D K+A PPPP P PLPPC
Sbjct: 23 VVATQCHQQPAQHREDQSKSAYHHPPPP-----------PPPPLPPC 130
>TC79235 weakly similar to GP|7297741|gb|AAF52992.1| CG17108 gene product
{Drosophila melanogaster}, partial (21%)
Length = 648
Score = 30.8 bits (68), Expect = 0.39
Identities = 24/69 (34%), Positives = 25/69 (35%)
Frame = -2
Query: 46 PPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCT 105
PPP P P P AP PP H PP NP Y P+ HHH T
Sbjct: 449 PPPMPHSHHPPPPHDPFAPPPPVFHHP--PPPPNP---YAPPPV----------VHHHHT 315
Query: 106 PLTVRNKTP 114
PL P
Sbjct: 314 PLHAHPAPP 288
>TC77892 similar to GP|14861486|gb|AAK73693.1 succinate dehydrogenase
subunit 3 {Glycine max}, partial (92%)
Length = 1148
Score = 30.8 bits (68), Expect = 0.39
Identities = 18/39 (46%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +2
Query: 61 PRAPLPPCLLHEAMKPPRNPSR-RYLQSPISGEPLTIWR 98
P PLPP + PP P+R + LQ IS LTIWR
Sbjct: 161 PSLPLPPPQNNSTAPPPLFPTRIKTLQRDISVAYLTIWR 277
>TC82658 homologue to GP|5917666|gb|AAD55980.1| extensin-like protein {Zea
mays}, partial (1%)
Length = 776
Score = 30.8 bits (68), Expect = 0.39
Identities = 15/30 (50%), Positives = 16/30 (53%)
Frame = +1
Query: 97 WRNQHHHCTPLTVRNKTPQEIFRFAALRRR 126
W NQ HH P V NKTP I + L RR
Sbjct: 268 WENQTHHILPRDVSNKTPMFILQ**QLCRR 357
Score = 29.6 bits (65), Expect = 0.88
Identities = 18/58 (31%), Positives = 20/58 (34%)
Frame = +1
Query: 101 HHHCTPLTVRNKTPQEIFRFAALRRRASALQLAISPSFGLRWPRNHHHHALHGERNKT 158
HHH PL K+PQ IFR L + W HH NKT
Sbjct: 145 HHH*IPLFFLYKSPQFIFRLKKFNF*DLPLLSCFPATVSCHWENQTHHILPRDVSNKT 318
>BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, partial (2%)
Length = 1320
Score = 30.8 bits (68), Expect = 0.39
Identities = 19/48 (39%), Positives = 22/48 (45%)
Frame = -3
Query: 41 AATRKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSP 88
A +PPPP LL L P PLPP PPR PS+ + P
Sbjct: 685 AVAIRPPPPLLLL----LLAPAPPLPP----PPYPPPRRPSKPPARPP 566
Score = 26.9 bits (58), Expect = 5.7
Identities = 26/93 (27%), Positives = 38/93 (39%)
Frame = -1
Query: 51 TLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQSPISGEPLTIWRNQHHHCTPLTVR 110
TLL+ + P + +P C RR L+S I ++R+ H
Sbjct: 969 TLLVSSSILYPLSHMPRC-------------RRILRSTI------LFRSPH--------- 874
Query: 111 NKTPQEIFRFAALRRRASALQLAISPSFGLRWP 143
+P R A L + L L+ SPSF LR+P
Sbjct: 873 -VSPSPAARAACLSACITRLFLSFSPSFSLRYP 778
>BQ079360 homologue to SP|Q40358|PRP_ Repetitive proline-rich cell wall
protein precursor (MSPRP). [Alfalfa] {Medicago sativa},
partial (55%)
Length = 493
Score = 30.4 bits (67), Expect = 0.51
Identities = 19/75 (25%), Positives = 29/75 (38%)
Frame = +3
Query: 3 SFFTFLFMCLILEIVQTMKNKISTAVVLTRCYRDDTRKAATRKPPPPRTLLLPFPLFQPR 62
S F L + + I Q + N V Y+ K KPP + + P+++P
Sbjct: 15 SNFLVLLLFALFAIPQGLANYEKPPVYQPPVYKPPVEKPPVYKPPVEKPPVYKPPVYKPP 194
Query: 63 APLPPCLLHEAMKPP 77
PP +KPP
Sbjct: 195VYKPPVYKPPVVKPP 239
>BQ144306 weakly similar to GP|23093252|gb| CG13731-PA {Drosophila
melanogaster}, partial (4%)
Length = 1261
Score = 30.4 bits (67), Expect = 0.51
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = -1
Query: 46 PPPPRTLLLPFPLFQPRAPLPP 67
PPPP TL LP P P P PP
Sbjct: 493 PPPPHTLHLPSPRQHPPRPPPP 428
>AL369112 weakly similar to GP|13540393|gb| histone H1 {Pisum sativum},
partial (20%)
Length = 371
Score = 30.4 bits (67), Expect = 0.51
Identities = 17/44 (38%), Positives = 23/44 (51%)
Frame = +3
Query: 44 RKPPPPRTLLLPFPLFQPRAPLPPCLLHEAMKPPRNPSRRYLQS 87
R+PPPP L+P + P PP +PPR+ RR L+S
Sbjct: 99 RRPPPPLRRLMPARRTRRPTPKPP--RSRLRRPPRSLPRRPLRS 224
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.139 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,235,677
Number of Sequences: 36976
Number of extensions: 178967
Number of successful extensions: 3464
Number of sequences better than 10.0: 172
Number of HSP's better than 10.0 without gapping: 2529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3253
length of query: 210
length of database: 9,014,727
effective HSP length: 92
effective length of query: 118
effective length of database: 5,612,935
effective search space: 662326330
effective search space used: 662326330
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0021.5