BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0019b.10
(251 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91283 weakly similar to GP|21554159|gb|AAM63238.1 unknown {Ara... 34 0.047
TC79359 weakly similar to PIR|S49915|S49915 extensin-like protei... 33 0.14
TC89613 similar to GP|14334448|gb|AAK59422.1 unknown protein {Ar... 33 0.14
BQ143784 weakly similar to SP|P33485|VNUA Probable nuclear antig... 32 0.18
BQ751748 similar to PIR|T47216|T47 probable V-ATPase 20K chain ... 32 0.30
TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like... 32 0.30
TC80483 similar to GP|22136520|gb|AAM91338.1 putative protein {A... 31 0.52
TC89403 similar to GP|17065074|gb|AAL32691.1 RNA-binding protein... 26 0.53
TC77972 similar to GP|6175162|gb|AAF04888.1| hypothetical protei... 30 0.67
TC83013 similar to GP|21554159|gb|AAM63238.1 unknown {Arabidopsi... 30 1.1
AW690594 similar to GP|23498163|emb hypothetical protein {Plasmo... 29 2.0
TC78237 similar to GP|17933299|gb|AAL48232.1 AT4g17800/dl4935c {... 29 2.0
TC80273 similar to GP|8978267|dbj|BAA98158.1 contains similarity... 29 2.0
TC91718 similar to GP|6175162|gb|AAF04888.1| hypothetical protei... 29 2.0
TC88820 homologue to PIR|D84890|D84890 probable AT-hook DNA-bind... 29 2.0
TC92402 similar to GP|20198252|gb|AAM15483.1 subtilisin-like ser... 28 2.6
TC87439 weakly similar to GP|5139695|dbj|BAA81686.1 expressed in... 28 2.6
TC91673 similar to GP|21741497|emb|CAD40673. OSJNBb0118P14.11 {O... 28 3.3
TC86498 similar to GP|5731259|gb|AAD48837.1| alanine:glyoxylate ... 28 3.3
TC86865 similar to PIR|E96497|E96497 probable bZIP transcription... 28 3.3
>TC91283 weakly similar to GP|21554159|gb|AAM63238.1 unknown {Arabidopsis
thaliana}, partial (31%)
Length = 784
Score = 34.3 bits (77), Expect = 0.047
Identities = 33/123 (26%), Positives = 51/123 (40%), Gaps = 11/123 (8%)
Frame = +2
Query: 138 LPFLTSQQNRLSGFATTSATSPTSELREITNVFV---RTSPHQIIRQRASPSATVRNAS- 193
LPF T +S F+ + S + N R P +II +R S A+ + +
Sbjct: 158 LPFFTQHPKNISSFSRNMSNRWWSGRQTEANPVEGENRNGPTRIILRRESRRASNGSVNG 337
Query: 194 -------SPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNK 246
+P S+ S GHV + + + +S + + RGRP GSKNK
Sbjct: 338 NVTGGNVTPTRSNTSNTGTGNSNGHVN--DELENSNGRSGDQTARSGRRPRGRPPGSKNK 511
Query: 247 PQP 249
P+P
Sbjct: 512 PKP 520
>TC79359 weakly similar to PIR|S49915|S49915 extensin-like protein - maize,
partial (3%)
Length = 1170
Score = 32.7 bits (73), Expect = 0.14
Identities = 27/96 (28%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
Frame = +3
Query: 155 SATSPTSELREITNVFVRTSPHQIIRQRASPSATVRNASSPETSSQQCFPLTCSPGHVAS 214
S +S TS++ ++ ++FV + ++ + +P ASSP++S +T P S
Sbjct: 138 SKSSKTSKMNKLFSLFVLSMLFTVLFAQNAP------ASSPKSS------VTAKPPSSVS 281
Query: 215 VEPV-TPAVKKSSNTNSSKKSSQRGRPKGSKNKPQP 249
V P +PA S T S + P GS + P P
Sbjct: 282 VSPTNSPASPAKSPTLSPPSQTPAVSPSGSASTPPP 389
>TC89613 similar to GP|14334448|gb|AAK59422.1 unknown protein {Arabidopsis
thaliana}, partial (54%)
Length = 1706
Score = 32.7 bits (73), Expect = 0.14
Identities = 29/124 (23%), Positives = 49/124 (39%), Gaps = 18/124 (14%)
Frame = +2
Query: 144 QQNRLSGFATTSATSPTSELREITNVFVRTSPHQIIRQRASPSATVRNASSPETSSQ--- 200
Q+ R +T+ +PT I V RT H++ R + T N SSP+ Q
Sbjct: 53 QRARKRESRSTTTHNPTRRWHRIRLVQSRTWRHRLHRPQPPSLKTTTNPSSPKRPPQTPK 232
Query: 201 ---------------QCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKN 245
Q FPL S S + P+ +++ S + S+R P+ ++
Sbjct: 233 FPPYPPFKDPLRQHHQHFPLHNSSKSHRSNPILRPSTNRTNPQRSRSRRSRRSFPQATRT 412
Query: 246 KPQP 249
+ +P
Sbjct: 413 RDEP 424
>BQ143784 weakly similar to SP|P33485|VNUA Probable nuclear antigen. [strain
Kaplan PRV] {Pseudorabies virus}, partial (2%)
Length = 1022
Score = 32.3 bits (72), Expect = 0.18
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
Frame = +3
Query: 180 RQRASPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGR 239
R+R +P + R + P Q P H S+ P K + TN K+ QRGR
Sbjct: 351 RERRAPGKSHRKSKKPPQEKTQ-------PPHHHSIRP------KKNWTN*KKEGGQRGR 491
Query: 240 PKGSKNK-PQPHP 251
P+ ++++ P P P
Sbjct: 492 PRTTQDRNPTPPP 530
>BQ751748 similar to PIR|T47216|T47 probable V-ATPase 20K chain [imported] -
Neurospora crassa, partial (82%)
Length = 642
Score = 31.6 bits (70), Expect = 0.30
Identities = 22/66 (33%), Positives = 31/66 (46%)
Frame = -1
Query: 184 SPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGS 243
SP T+ P TSS+ C LT AS P TPAV +++ +SS + P S
Sbjct: 387 SPMVTLSP*PRPVTSSRACPSLTSPTSGPASTAPSTPAV--AASASSS*TTPVASSPSTS 214
Query: 244 KNKPQP 249
++ P
Sbjct: 213 RSSTAP 196
>TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like protein
{Arabidopsis thaliana}, partial (16%)
Length = 814
Score = 31.6 bits (70), Expect = 0.30
Identities = 21/77 (27%), Positives = 28/77 (36%), Gaps = 8/77 (10%)
Frame = +3
Query: 183 ASPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSN--------TNSSKKS 234
A P +T SSP S+ P +P + P TPA+ S + + S
Sbjct: 78 APPPSTPATPSSPPPSTTPSAPPPSTPSNSPPSPPTTPAISPPSGGGTTPSPPSRTPPSS 257
Query: 235 SQRGRPKGSKNKPQPHP 251
P SK P P P
Sbjct: 258 DDSPSPPSSKTPPPPSP 308
>TC80483 similar to GP|22136520|gb|AAM91338.1 putative protein {Arabidopsis
thaliana}, partial (32%)
Length = 991
Score = 30.8 bits (68), Expect = 0.52
Identities = 16/36 (44%), Positives = 22/36 (60%), Gaps = 3/36 (8%)
Frame = +1
Query: 65 IEGWKNNLIVE--GILFNEN-QIFDNYVQNQGTRYA 97
+EGW N E G+L EN ++ D Y+ NQGT Y+
Sbjct: 793 VEGWPNEETEELKGVLEKENLKVKDQYLSNQGTLYS 900
>TC89403 similar to GP|17065074|gb|AAL32691.1 RNA-binding protein MEI2
putative {Arabidopsis thaliana}, partial (13%)
Length = 952
Score = 25.8 bits (55), Expect(2) = 0.53
Identities = 18/54 (33%), Positives = 25/54 (45%)
Frame = -1
Query: 171 VRTSPHQIIRQRASPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKK 224
V SP+ R R+S S+T+ + C P CSP A+ P TP K+
Sbjct: 835 VLISPYCSKRVRSSVSSTLLLIFRTNNVREGCSPYGCSP---ATAPPPTPLGKE 683
Score = 23.5 bits (49), Expect(2) = 0.53
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -2
Query: 217 PVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQP 249
P P + K S SSK S + G+P+ SK+ P
Sbjct: 591 PSLPLLSKRSY--SSKSSREPGKPRKSKSSIMP 499
>TC77972 similar to GP|6175162|gb|AAF04888.1| hypothetical protein
{Arabidopsis thaliana}, partial (46%)
Length = 1589
Score = 30.4 bits (67), Expect = 0.67
Identities = 14/22 (63%), Positives = 17/22 (76%)
Frame = +2
Query: 228 TNSSKKSSQRGRPKGSKNKPQP 249
TN+S + RGRP GSKNKP+P
Sbjct: 539 TNTSTRRP-RGRPSGSKNKPKP 601
>TC83013 similar to GP|21554159|gb|AAM63238.1 unknown {Arabidopsis
thaliana}, partial (24%)
Length = 954
Score = 29.6 bits (65), Expect = 1.1
Identities = 13/21 (61%), Positives = 15/21 (70%)
Frame = +1
Query: 229 NSSKKSSQRGRPKGSKNKPQP 249
+SS RGRP GSKNKP+P
Sbjct: 709 SSSGGRRPRGRPPGSKNKPKP 771
>AW690594 similar to GP|23498163|emb hypothetical protein {Plasmodium
falciparum 3D7}, partial (10%)
Length = 633
Score = 28.9 bits (63), Expect = 2.0
Identities = 17/63 (26%), Positives = 31/63 (48%)
Frame = -3
Query: 183 ASPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKG 242
+SPS++ ++SS +SS +CS +S + + SS+++S SS P
Sbjct: 244 SSPSSSSSSSSSSSSSSSSSSSSSCSSSSSSSSSSSSSSSCSSSSSSSCSSSSSSTSPSS 65
Query: 243 SKN 245
S +
Sbjct: 64 SSS 56
>TC78237 similar to GP|17933299|gb|AAL48232.1 AT4g17800/dl4935c {Arabidopsis
thaliana}, partial (59%)
Length = 1166
Score = 28.9 bits (63), Expect = 2.0
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = +3
Query: 237 RGRPKGSKNKPQP 249
RGRP GSKNKP+P
Sbjct: 273 RGRPPGSKNKPKP 311
>TC80273 similar to GP|8978267|dbj|BAA98158.1 contains similarity to AT-hook
DNA-binding protein~gene_id:K2I5.6 {Arabidopsis
thaliana}, partial (53%)
Length = 1418
Score = 28.9 bits (63), Expect = 2.0
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = +3
Query: 237 RGRPKGSKNKPQP 249
RGRP GSKNKP+P
Sbjct: 420 RGRPPGSKNKPKP 458
>TC91718 similar to GP|6175162|gb|AAF04888.1| hypothetical protein
{Arabidopsis thaliana}, partial (41%)
Length = 449
Score = 28.9 bits (63), Expect = 2.0
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = +3
Query: 237 RGRPKGSKNKPQP 249
RGRP GSKNKP+P
Sbjct: 99 RGRPPGSKNKPKP 137
>TC88820 homologue to PIR|D84890|D84890 probable AT-hook DNA-binding protein
[imported] - Arabidopsis thaliana, partial (58%)
Length = 1544
Score = 28.9 bits (63), Expect = 2.0
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = +3
Query: 237 RGRPKGSKNKPQP 249
RGRP GSKNKP+P
Sbjct: 759 RGRPAGSKNKPKP 797
>TC92402 similar to GP|20198252|gb|AAM15483.1 subtilisin-like serine
protease AIR3 {Arabidopsis thaliana}, partial (16%)
Length = 704
Score = 28.5 bits (62), Expect = 2.6
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2
Query: 7 AYWKVSRFVASQRRTGCWLLLAWCYR 32
A W+V ++A + R WL LA CY+
Sbjct: 376 AMWEVCHYIAHKHR*KIWLQLASCYK 299
>TC87439 weakly similar to GP|5139695|dbj|BAA81686.1 expressed in cucumber
hypocotyls {Cucumis sativus}, partial (57%)
Length = 897
Score = 28.5 bits (62), Expect = 2.6
Identities = 31/133 (23%), Positives = 50/133 (37%), Gaps = 14/133 (10%)
Frame = +3
Query: 133 GIGACLPFLTSQQNRLSGFATTSATSP-----TSELREITNVFVRTSPHQIIRQRASPSA 187
G+G P + + ATT +SP T+ ++SP + A+P+A
Sbjct: 90 GVGGQSPSAAPTTSPSTPAATTPVSSPVAAPPTTPTTPAPVASPKSSPPATSPKAAAPTA 269
Query: 188 TVRNASSPETSSQQCFPLTC--------SPGHVASVEPVTPAVKKSSNTNSSKKSSQRGR 239
T ASSP + P +P V+S VTP ++ + +G+
Sbjct: 270 TPPAASSPPAVTPVSTPPPAPVPVKSPPTPAPVSSPPAVTPVAAPTTTPAVPAPAPSKGK 449
Query: 240 PKGSKN-KPQPHP 251
K+ P P P
Sbjct: 450 KNKKKHGAPAPSP 488
>TC91673 similar to GP|21741497|emb|CAD40673. OSJNBb0118P14.11 {Oryza
sativa}, partial (10%)
Length = 733
Score = 28.1 bits (61), Expect = 3.3
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +3
Query: 12 SRFVASQRRTGCWLLLAWCYRRGKIQT 38
S++ RRT +LLL WC + K+ T
Sbjct: 363 SKYALEMRRTSSFLLLVWCCLKCKLYT 443
>TC86498 similar to GP|5731259|gb|AAD48837.1| alanine:glyoxylate
aminotransferase 2 homolog {Arabidopsis thaliana},
partial (91%)
Length = 1956
Score = 28.1 bits (61), Expect = 3.3
Identities = 17/59 (28%), Positives = 25/59 (41%), Gaps = 17/59 (28%)
Frame = +1
Query: 98 VTGYSFNAQDLIQKS-----------------NNYWPFYSLNLDPRILTQTNGIGACLP 139
V GY N D++ K+ ++YW F + + P I+T GIG LP
Sbjct: 1057 VPGYLKNVYDIVHKAGGVCIADEVQTGFGRTGSHYWGFETQGVIPDIVTMAKGIGNGLP 1233
>TC86865 similar to PIR|E96497|E96497 probable bZIP transcription factor
[imported] - Arabidopsis thaliana, partial (28%)
Length = 1171
Score = 28.1 bits (61), Expect = 3.3
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +3
Query: 209 PGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQ 248
PGH + V +S T+ K SQR + GSK Q
Sbjct: 723 PGHHVPTQSTPSTVANNSVTSKRKHGSQRSKKGGSKRAKQ 842
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.129 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,691,288
Number of Sequences: 36976
Number of extensions: 98285
Number of successful extensions: 822
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 806
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 819
length of query: 251
length of database: 9,014,727
effective HSP length: 94
effective length of query: 157
effective length of database: 5,538,983
effective search space: 869620331
effective search space used: 869620331
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0019b.10