BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0018.1
(127 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77388 similar to PIR|E96553|E96553 probable RNA-binding protei... 88 8e-19
CA859470 similar to GP|11034807|gb| RNA-binding protein Y14 {Xen... 51 1e-07
TC92312 weakly similar to GP|4567275|gb|AAD23688.1| expressed pr... 36 0.005
TC88545 similar to GP|13560783|gb|AAK30205.1 poly(A)-binding pro... 33 0.023
CA859848 similar to GP|12842203|dbj data source:MGD source key:... 33 0.039
TC76331 similar to GP|7673355|gb|AAF66823.1| poly(A)-binding pro... 32 0.066
TC77533 similar to GP|6996560|emb|CAB75429.1 oligouridylate bind... 32 0.066
BG644387 homologue to GP|6996560|emb oligouridylate binding prot... 31 0.11
TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding pro... 31 0.15
AL388260 weakly similar to SP|Q08937|ROC2_ 29 kDa ribonucleoprot... 30 0.19
TC89748 homologue to PIR|B84565|B84565 probable spliceosome asso... 30 0.25
TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 30 0.33
TC87747 weakly similar to PIR|C84768|C84768 hypothetical protein... 30 0.33
TC90607 similar to GP|20466724|gb|AAM20679.1 unknown protein {Ar... 30 0.33
TC88887 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproli... 29 0.56
TC83666 similar to GP|15042832|gb|AAK82455.1 putative RNA bindin... 29 0.56
TC81507 similar to GP|22831146|dbj|BAC16007. putative pre-mRNA s... 28 0.73
TC89001 similar to GP|5726567|gb|AAD48471.1| glycine-rich RNA-bi... 28 0.95
TC77170 weakly similar to PIR|T06154|T06154 hypothetical protein... 28 1.2
AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding pr... 28 1.2
>TC77388 similar to PIR|E96553|E96553 probable RNA-binding protein
35994-37391 [imported] - Arabidopsis thaliana, partial
(64%)
Length = 1029
Score = 88.2 bits (217), Expect = 8e-19
Identities = 41/47 (87%), Positives = 45/47 (95%)
Frame = +2
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSGPINESIRR 47
GYALIEYER+EEA+NAIENLNGS LLTQ IYVDWAFSSGPINE++RR
Sbjct: 455 GYALIEYERSEEARNAIENLNGSVLLTQTIYVDWAFSSGPINETVRR 595
>CA859470 similar to GP|11034807|gb| RNA-binding protein Y14 {Xenopus
laevis}, partial (43%)
Length = 686
Score = 50.8 bits (120), Expect = 1e-07
Identities = 23/45 (51%), Positives = 32/45 (71%)
Frame = +3
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSGPINESI 45
GYAL+EYE +EAKNAIE +NG++LL ++ D+AF P S+
Sbjct: 378 GYALVEYETYKEAKNAIETVNGTKLLGSTLHCDFAFIRAPAAPSV 512
>TC92312 weakly similar to GP|4567275|gb|AAD23688.1| expressed protein
{Arabidopsis thaliana}, partial (29%)
Length = 1319
Score = 35.8 bits (81), Expect = 0.005
Identities = 15/35 (42%), Positives = 25/35 (70%)
Frame = +1
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWA 35
G+A +++ ++A+NAI LNGS+ ++ I VDWA
Sbjct: 400 GFAFVKFTCKQDAENAIRKLNGSKFGSRLIAVDWA 504
>TC88545 similar to GP|13560783|gb|AAK30205.1 poly(A)-binding protein
{Daucus carota}, partial (35%)
Length = 866
Score = 33.5 bits (75), Expect = 0.023
Identities = 20/62 (32%), Positives = 29/62 (46%)
Frame = +2
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSGPINESIRRKNARHPVMNLNIY 60
GY +++E E A++AI+ LNG L + +YV G R NA N+Y
Sbjct: 566 GYGFVQFENEESAQSAIDKLNGMLLNDKPVYV------GHFQRKQDRDNALSNAKFNNVY 727
Query: 61 FK 62
K
Sbjct: 728 VK 733
>CA859848 similar to GP|12842203|dbj data source:MGD source key:MGI:1917118
evidence:ISS~peptidylprolyl isomerase E (cyclophilin
E)~, partial (30%)
Length = 387
Score = 32.7 bits (73), Expect = 0.039
Identities = 16/35 (45%), Positives = 24/35 (67%)
Frame = +2
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWA 35
G+ IE+E A +A+ AI+N+N SEL + I V+ A
Sbjct: 170 GFGFIEFEEAADAQEAIDNMNLSELYGKVIKVNLA 274
>TC76331 similar to GP|7673355|gb|AAF66823.1| poly(A)-binding protein
{Nicotiana tabacum}, partial (97%)
Length = 2581
Score = 32.0 bits (71), Expect = 0.066
Identities = 18/62 (29%), Positives = 29/62 (46%)
Frame = +3
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSGPINESIRRKNARHPVMNLNIY 60
GY ++++ E A+ AIE LNG L + +YV GP R++ N++
Sbjct: 831 GYGFVQFDTEEAAQKAIEKLNGMLLNDKQVYV------GPFLRKQERESTGDRAKFNNVF 992
Query: 61 FK 62
K
Sbjct: 993 VK 998
>TC77533 similar to GP|6996560|emb|CAB75429.1 oligouridylate binding protein
{Nicotiana plumbaginifolia}, partial (79%)
Length = 1621
Score = 32.0 bits (71), Expect = 0.066
Identities = 14/38 (36%), Positives = 21/38 (54%)
Frame = +1
Query: 2 YALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSG 39
Y ++Y A AI LNG + Q+I V+WA++ G
Sbjct: 337 YGFVDYFDRSSAAIAIVTLNGRNIFGQSIKVNWAYTRG 450
>BG644387 homologue to GP|6996560|emb oligouridylate binding protein
{Nicotiana plumbaginifolia}, partial (53%)
Length = 717
Score = 31.2 bits (69), Expect = 0.11
Identities = 15/37 (40%), Positives = 20/37 (53%)
Frame = +2
Query: 2 YALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSS 38
Y ++Y A AI LNG L Q I V+WA++S
Sbjct: 236 YGFVDYFDRRSAALAIVTLNGRNLFGQPIKVNWAYTS 346
>TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding protein
{Cucumis sativus}, partial (90%)
Length = 2501
Score = 30.8 bits (68), Expect = 0.15
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +3
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYV 32
GY +++E + A+NAI+ LNG + + ++V
Sbjct: 681 GYGFVQFEAEDSAQNAIDKLNGMLINDKQVFV 776
Score = 25.0 bits (53), Expect = 8.0
Identities = 9/32 (28%), Positives = 18/32 (56%)
Frame = +3
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYV 32
G + + EEA A+ +NG ++++ +YV
Sbjct: 1266 GSGFVAFSTPEEASRALGEMNGKMIVSKPLYV 1361
>AL388260 weakly similar to SP|Q08937|ROC2_ 29 kDa ribonucleoprotein B
chloroplast precursor (CP29B). [Wood tobacco] {Nicotiana
sylvestris}, partial (26%)
Length = 346
Score = 30.4 bits (67), Expect = 0.19
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +3
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWA 35
G+ + + EEA+ AI+NLN +E +NI VD A
Sbjct: 210 GFGFVTFASNEEAEIAIQNLNDAEFDGRNIKVDRA 314
>TC89748 homologue to PIR|B84565|B84565 probable spliceosome associated
protein [imported] - Arabidopsis thaliana, partial (56%)
Length = 707
Score = 30.0 bits (66), Expect = 0.25
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +2
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAF 36
G+ I Y+ E + +AIE +NG L + I V +A+
Sbjct: 554 GFGFISYDSFEASDSAIEAMNGQYLCNRQITVSYAY 661
>TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (69%)
Length = 1615
Score = 29.6 bits (65), Expect = 0.33
Identities = 15/39 (38%), Positives = 25/39 (63%)
Frame = +3
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSG 39
G+ +E+ AE A++A+E +NG ELL + + +D A G
Sbjct: 672 GFGHVEFASAEAAQSALE-MNGQELLQRAVRLDLARERG 785
>TC87747 weakly similar to PIR|C84768|C84768 hypothetical protein At2g35410
[imported] - Arabidopsis thaliana, partial (53%)
Length = 1177
Score = 29.6 bits (65), Expect = 0.33
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = +1
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYV 32
GY + Y EEA+ AI L G ELL + + V
Sbjct: 715 GYGFVSYHTEEEAEAAISALQGKELLGRPLLV 810
>TC90607 similar to GP|20466724|gb|AAM20679.1 unknown protein {Arabidopsis
thaliana}, partial (49%)
Length = 1143
Score = 29.6 bits (65), Expect = 0.33
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = +1
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSGP 40
GYA IEY E A A++ +NG + I VD A ++ P
Sbjct: 703 GYAFIEYTTEEAASAALKEMNGKIINGWMIVVDVAKTTPP 822
>TC88887 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproline-rich
glycoprotein DZ-HRGP {Volvox carteri f. nagariensis},
partial (18%)
Length = 1681
Score = 28.9 bits (63), Expect = 0.56
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -2
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYV 32
G ++EY E+A+NA+ L+ L+ + +YV
Sbjct: 1236 GCGIVEYATREQAQNAVATLSNQNLMGRLVYV 1141
>TC83666 similar to GP|15042832|gb|AAK82455.1 putative RNA binding protein
{Oryza sativa}, partial (52%)
Length = 896
Score = 28.9 bits (63), Expect = 0.56
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVD 33
G+A I YE A++NLNG+++ + I VD
Sbjct: 394 GFAFIAYEDQRSTNLAVDNLNGAQVSGRIIRVD 492
>TC81507 similar to GP|22831146|dbj|BAC16007. putative pre-mRNA splicing
factor SF2 (SR1 protein) {Oryza sativa (japonica
cultivar-group)}, partial (57%)
Length = 1287
Score = 28.5 bits (62), Expect = 0.73
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +2
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSGPINESIRRKNARH 52
GYA +E+E ++A++AI +G + + V+ A G N S R + + H
Sbjct: 227 GYAFVEFEDVQDAEDAIRGRDGYDFDGHRLRVE-AAHGGRGNSSSRDRYSSH 379
>TC89001 similar to GP|5726567|gb|AAD48471.1| glycine-rich RNA-binding
protein {Glycine max}, partial (90%)
Length = 488
Score = 28.1 bits (61), Expect = 0.95
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = +3
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSG 39
G+ + + + ++AIE +NG ++ +NI V+ A S G
Sbjct: 177 GFGFVTFAEEKSMRDAIEEMNGQDIDGRNITVNEAQSRG 293
>TC77170 weakly similar to PIR|T06154|T06154 hypothetical protein F24J7.162
- Arabidopsis thaliana, partial (23%)
Length = 1059
Score = 27.7 bits (60), Expect = 1.2
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = +1
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSGPINESIRRKNA 50
G+A +EY +EA+NA+ L+ + +++ ++ A G E +R + A
Sbjct: 628 GFAFVEYVTQQEAQNALTALSSTHSYGRHLVIERA-KEGESLEELRARTA 774
>AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding protein
{Medicago sativa}, complete
Length = 437
Score = 27.7 bits (60), Expect = 1.2
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +1
Query: 1 GYALIEYERAEEAKNAIENLNGSELLTQNIYVDWAFSSG 39
G+ + + + +AIE +NG +L +NI V+ A S G
Sbjct: 184 GFGFVTFANEKSMNDAIEAMNGQDLDGRNITVNQAQSRG 300
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.141 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,086,484
Number of Sequences: 36976
Number of extensions: 56083
Number of successful extensions: 346
Number of sequences better than 10.0: 72
Number of HSP's better than 10.0 without gapping: 342
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 346
length of query: 127
length of database: 9,014,727
effective HSP length: 85
effective length of query: 42
effective length of database: 5,871,767
effective search space: 246614214
effective search space used: 246614214
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0018.1