BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0014.4
(568 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 77 2e-14
BG586862 61 1e-09
BG585866 53 4e-07
BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At... 52 6e-07
TC93101 48 1e-05
AW980456 40 0.002
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 36 0.035
BG585499 33 0.23
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 33 0.29
TC76445 similar to PIR|T07623|T07623 extensin homolog HRGP2 - so... 32 0.66
AW774782 homologue to GP|13430186|gb ribosomal protein L33 {Cast... 32 0.86
BE240502 similar to GP|17226329|gb| chitinase-like protein 1 {Ar... 32 0.86
CA922381 homologue to GP|13430186|gb ribosomal protein L33 {Cast... 32 0.86
TC85443 homologue to GP|13430186|gb|AAK25760.1 ribosomal protein... 32 0.86
BF520135 31 1.1
BG646730 31 1.1
BF521488 weakly similar to GP|18447433|gb RE28280p {Drosophila m... 30 1.9
CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [i... 30 3.3
BE997422 similar to GP|9758023|dbj ABC transporter-like protein ... 30 3.3
TC82560 weakly similar to GP|7406712|emb|CAB85635.1 putative rip... 30 3.3
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 77.0 bits (188), Expect = 2e-14
Identities = 40/114 (35%), Positives = 56/114 (49%), Gaps = 4/114 (3%)
Frame = +3
Query: 212 WMGTVDDVYTVKTGYE----WH*QLENQNCPSSSNEVGLEPAQWEFFWKAPSFPRVREVA 267
WM +Y+VK+GY W Q N SS E W+ W + PR + +
Sbjct: 6 WMHNPTGIYSVKSGYNTLRTWQTQQINNTSTSSD-----ETLIWKKIWSLHTIPRHKVLL 170
Query: 268 WRVCTWAVPVRVKLHRRGVDVDPSYPLCGLEDEIVEHLFMSCNVSRGCWFARNL 321
WR+ ++PVR L +RG+ P P C + E + HLFMSC +S+ WF NL
Sbjct: 171 WRILNDSLPVRSSLRKRGIQCYPLCPRCHSKTETITHLFMSCPLSKRVWFGSNL 332
>BG586862
Length = 804
Score = 61.2 bits (147), Expect = 1e-09
Identities = 29/80 (36%), Positives = 44/80 (54%)
Frame = -1
Query: 252 EFFWKAPSFPRVREVAWRVCTWAVPVRVKLHRRGVDVDPSYPLCGLEDEIVEHLFMSCNV 311
E W + PR + WR+ A+PV+ +LH+RG+ P C + E V+HLF++C V
Sbjct: 660 EKVWGIKTIPRHKSFLWRLLHNALPVKDELHKRGIRCSLLCPRCESKIETVQHLFLNCEV 481
Query: 312 SRGCWFARNLGLRLLSGSVL 331
++ WF LG+ S VL
Sbjct: 480 TQKEWFGSQLGINFHSSGVL 421
>BG585866
Length = 828
Score = 52.8 bits (125), Expect = 4e-07
Identities = 44/182 (24%), Positives = 66/182 (36%), Gaps = 7/182 (3%)
Frame = +3
Query: 142 WEDNWLPNGSPIKFPQEVGDEQRLVRVADLFLP-----NHGGWNTPLVE*TFFPTMVARI 196
W NW G +G + V + DL L GG +T + T PT +A +
Sbjct: 153 WYTNWSSLGL-------LGTQAPFVDIHDLHLTVKDVFTTGGQHTQSLY-TILPTDIAEV 308
Query: 197 L--VTLIRHQDVVDCLFWMGTVDDVYTVKTGYEWH*QLENQNCPSSSNEVGLEPAQWEFF 254
+ L + + D W + VYT K+GY W S + V + W +
Sbjct: 309 INNTHLNFNASIGDAYIWPHNSNGVYTAKSGYSW--------ILSQTETVNYNNSSWSWI 464
Query: 255 WKAPSFPRVREVAWRVCTWAVPVRVKLHRRGVDVDPSYPLCGLEDEIVEHLFMSCNVSRG 314
W+ + + W C AVP L+ R + CG +E H C S+
Sbjct: 465 WRLKIPEKYKFFLWLACHNAVPTLSLLNHRNMVNSAICSRCGEHEESFFHCVRDCRFSKI 644
Query: 315 CW 316
W
Sbjct: 645 IW 650
>BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (10%)
Length = 767
Score = 52.0 bits (123), Expect = 6e-07
Identities = 31/114 (27%), Positives = 52/114 (45%), Gaps = 5/114 (4%)
Frame = -3
Query: 208 DCLFWMGTVDDVYTVKTGYEWH*QLENQNCPSSSNEVGL--EPAQWEFF---WKAPSFPR 262
D W + Y+VK+GY N +++N+ G +P+ + + WK + P+
Sbjct: 426 DSYSWEYSKSGHYSVKSGY-----YVQTNIIAAANQRGTVDQPSLDDLYQRVWKYNTSPK 262
Query: 263 VREVAWRVCTWAVPVRVKLHRRGVDVDPSYPLCGLEDEIVEHLFMSCNVSRGCW 316
VR WR + ++P + R + D S CG+E E V H+ C +R W
Sbjct: 261 VRHFLWRCISNSLPTAANMRSRHISKDGSCSRCGMESETVNHILFQCPYARLIW 100
>TC93101
Length = 675
Score = 47.8 bits (112), Expect = 1e-05
Identities = 21/57 (36%), Positives = 31/57 (53%)
Frame = -1
Query: 275 VPVRVKLHRRGVDVDPSYPLCGLEDEIVEHLFMSCNVSRGCWFARNLGLRLLSGSVL 331
+PVR +L++RGV+ P P C E H+FMSC ++ WF L +R S +
Sbjct: 663 LPVRXELNKRGVNCPPLCPRCYFNLETTNHIFMSCERTQRVWFGSQLSIRFPDNSTI 493
>AW980456
Length = 779
Score = 40.4 bits (93), Expect = 0.002
Identities = 21/78 (26%), Positives = 32/78 (40%), Gaps = 2/78 (2%)
Frame = -2
Query: 207 VDCLFWMGTVDDVYTVKTGYEWH*QLENQNCPSSSNEVGL--EPAQWEFFWKAPSFPRVR 264
+D L W Y VK+ Y + C + P W WK P+V+
Sbjct: 211 LDRLIWKDEKHGKYYVKSAYRF--------CVEELFDSSYLHRPGNWSGIWKLKVPPKVQ 56
Query: 265 EVAWRVCTWAVPVRVKLH 282
+ WR+C +P R++LH
Sbjct: 55 NLVWRMCRGCLPTRIRLH 2
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 36.2 bits (82), Expect = 0.035
Identities = 19/60 (31%), Positives = 30/60 (49%), Gaps = 3/60 (5%)
Frame = -2
Query: 255 WKAPSFPRVREVAWRVCTWAVPVRVKLHRRG---VDVDPSYPLCGLEDEIVEHLFMSCNV 311
WK+ + +V +W + +P V L +R V+ CG +DE V HLF+ C+V
Sbjct: 319 WKSKAPAKVLAFSWTLFLDRIPTMVNLGKRRLLRVEDSKRCVFCGCQDETVVHLFLHCDV 140
>BG585499
Length = 792
Score = 33.5 bits (75), Expect = 0.23
Identities = 17/66 (25%), Positives = 24/66 (35%)
Frame = +3
Query: 251 WEFFWKAPSFPRVREVAWRVCTWAVPVRVKLHRRGVDVDPSYPLCGLEDEIVEHLFMSCN 310
W+ W R + W V + + R G V + P CG DE V H+ C
Sbjct: 225 WKMLWGWRGPHRTQTFMWLVAHGCILTNYRRSRWGTRVLATCPCCGNADETVLHVLCDCR 404
Query: 311 VSRGCW 316
+ W
Sbjct: 405 PASQVW 422
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 33.1 bits (74), Expect = 0.29
Identities = 26/75 (34%), Positives = 36/75 (47%), Gaps = 4/75 (5%)
Frame = +2
Query: 262 RVREVAWRVCTWAVPVRVKLHRRGVDVDPSYP-LCGLED-EIVEHLFMSCNVSRGCW-FA 318
+V WR+ +P + L RGV + + +CG D E HLF+ CNV W
Sbjct: 650 KVSLFVWRLLRNRLPTKDNLVHRGVLLATNAACVCGCVDSESTTHLFLHCNVFCSLWSLV 829
Query: 319 RN-LGLRLLSGSVLR 332
RN LG+ +S LR
Sbjct: 830 RNWLGIPSMSSGELR 874
>TC76445 similar to PIR|T07623|T07623 extensin homolog HRGP2 - soybean
(fragment), partial (76%)
Length = 821
Score = 32.0 bits (71), Expect = 0.66
Identities = 16/46 (34%), Positives = 24/46 (51%), Gaps = 1/46 (2%)
Frame = -2
Query: 423 G*TWMLQWGWTSWRGLAWWRGITPVRCSQQLQTSQR-LLFLQRWLR 467
G T ++ W W WR WWRG V + + +R L+ ++RW R
Sbjct: 328 GRTHVMVWRWRRWRW--WWRGFVVVGRMRNGRWGRRTLIVIRRWWR 197
Score = 28.1 bits (61), Expect = 9.5
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -2
Query: 430 WGWTSWRGLAWWRG 443
WGW WR WWRG
Sbjct: 67 WGWRRWR---WWRG 35
>AW774782 homologue to GP|13430186|gb ribosomal protein L33 {Castanea
sativa}, complete
Length = 671
Score = 31.6 bits (70), Expect = 0.86
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = +3
Query: 450 SQQLQTSQRLLFLQRWLRL*ALSGLWNCLCILASGGFSLRRIAY 493
SQQL S + LF+ WL L + CLCIL SL RI++
Sbjct: 375 SQQLDRSLKFLFILFWLSL-----HFGCLCILVFFFVSLNRISF 491
>BE240502 similar to GP|17226329|gb| chitinase-like protein 1 {Arabidopsis
thaliana}, partial (27%)
Length = 560
Score = 31.6 bits (70), Expect = 0.86
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = +2
Query: 450 SQQLQTSQRLLFLQRWLRL*ALSGLWNCLCILASGGFSLRRIAY 493
SQQL S + LF+ WL L + CLCIL SL RI++
Sbjct: 104 SQQLDRSLKFLFILFWLSL-----HFGCLCILVFFFVSLNRISF 220
>CA922381 homologue to GP|13430186|gb ribosomal protein L33 {Castanea
sativa}, complete
Length = 803
Score = 31.6 bits (70), Expect = 0.86
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = -2
Query: 450 SQQLQTSQRLLFLQRWLRL*ALSGLWNCLCILASGGFSLRRIAY 493
SQQL S + LF+ WL L + CLCIL SL RI++
Sbjct: 190 SQQLDRSLKFLFILFWLSL-----HFGCLCILVFFFVSLNRISF 74
>TC85443 homologue to GP|13430186|gb|AAK25760.1 ribosomal protein L33
{Castanea sativa}, complete
Length = 773
Score = 31.6 bits (70), Expect = 0.86
Identities = 19/44 (43%), Positives = 24/44 (54%)
Frame = +2
Query: 450 SQQLQTSQRLLFLQRWLRL*ALSGLWNCLCILASGGFSLRRIAY 493
SQQL S + LF+ WL L + CLCIL SL RI++
Sbjct: 437 SQQLDRSLKFLFILFWLSL-----HFGCLCILVFFFVSLNRISF 553
>BF520135
Length = 202
Score = 31.2 bits (69), Expect = 1.1
Identities = 16/60 (26%), Positives = 29/60 (47%), Gaps = 3/60 (5%)
Frame = +3
Query: 255 WKAPSFPRVREVAWRVCTWAVPVRVKLHRRGVDVDPSYPLCGLEDEIVE---HLFMSCNV 311
W+ +V AWR+ +P + + +RG+ V +C ++E HLF+ C+V
Sbjct: 18 WRKEDLSKVSIFAWRLFHGRLPTKANVFKRGI-VHHDAHMCVTRCRLIESDVHLFLHCDV 194
>BG646730
Length = 799
Score = 31.2 bits (69), Expect = 1.1
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = +1
Query: 139 VRIWEDNWLPNGSPIKFPQEVGDEQRLVRVADLFLPNHGGWNTPLVE*TFFPTMVARIL 197
VRIW+D+WLPN + K + D + +++L N LV F +IL
Sbjct: 481 VRIWKDDWLPNQTGFKVCSPMVDFEEATCISELIDTNTKSSKRDLVRNVFSAFEAKQIL 657
>BF521488 weakly similar to GP|18447433|gb RE28280p {Drosophila
melanogaster}, partial (84%)
Length = 470
Score = 30.4 bits (67), Expect = 1.9
Identities = 7/17 (41%), Positives = 9/17 (52%)
Frame = +1
Query: 426 WMLQWGWTSWRGLAWWR 442
W ++W W W WWR
Sbjct: 256 WWIRWWWIRWSSFPWWR 306
>CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [imported] -
Sulfolobus solfataricus, partial (3%)
Length = 789
Score = 29.6 bits (65), Expect = 3.3
Identities = 23/80 (28%), Positives = 32/80 (39%), Gaps = 3/80 (3%)
Frame = -2
Query: 240 SSNEVGLEPAQWEFFWKAPSFPRVREVAWRVCTWAVPVRVKLHRRGV---DVDPSYPLCG 296
SS + L E W +V AWR+ +P + L RGV + CG
Sbjct: 626 SSGSLSLILPYAEMIWHRQVPLKVSVFAWRLLRDRLPTKSNLIYRGVIPTEAGLCVSGCG 447
Query: 297 LEDEIVEHLFMSCNVSRGCW 316
E +HLF+SC+ W
Sbjct: 446 AL-ESAQHLFLSCSYFASLW 390
>BE997422 similar to GP|9758023|dbj ABC transporter-like protein {Arabidopsis
thaliana}, partial (27%)
Length = 790
Score = 29.6 bits (65), Expect = 3.3
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = +1
Query: 475 WNCLCILASGGFSLRRIAYLSFRLGRR 501
WNCL + + GF R + YLS LG +
Sbjct: 550 WNCLWVTVAWGFFFRILFYLSLLLGSK 630
>TC82560 weakly similar to GP|7406712|emb|CAB85635.1 putative
ripening-related P-450 enzyme {Vitis vinifera}, partial
(18%)
Length = 792
Score = 29.6 bits (65), Expect = 3.3
Identities = 15/37 (40%), Positives = 23/37 (61%)
Frame = +3
Query: 512 MIVVV*FQFLIMLIFLLYVVRETLVLTTLPVTPLPFP 548
+++VV F +LIF++ V++T T LP P PFP
Sbjct: 39 LLLVVTFASASILIFIIRKVKQTQNSTKLPPGPNPFP 149
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.345 0.155 0.589
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,530,385
Number of Sequences: 36976
Number of extensions: 413444
Number of successful extensions: 4754
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 2576
Number of HSP's successfully gapped in prelim test: 207
Number of HSP's that attempted gapping in prelim test: 1877
Number of HSP's gapped (non-prelim): 3125
length of query: 568
length of database: 9,014,727
effective HSP length: 101
effective length of query: 467
effective length of database: 5,280,151
effective search space: 2465830517
effective search space used: 2465830517
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0014.4