BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0012a.1
(263 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89082 similar to PIR|H86476|H86476 protein F15O4.35 [imported]... 42 3e-04
TC83149 similar to GP|13561982|gb|AAK30594.1 flagelliform silk p... 33 0.11
TC92828 weakly similar to GP|4115538|dbj|BAA36412.1 UDP-glycose:... 33 0.15
BQ752265 similar to GP|8101036|gb| delta-1-pyrroline-5-carboxyla... 32 0.19
TC92700 weakly similar to PIR|T39956|T39956 probable nadh-depend... 31 0.43
TC80088 similar to GP|9759352|dbj|BAB10007.1 gene_id:MAH20.14~un... 31 0.56
BQ752418 homologue to SP|P78695|GR78 78 kDa glucose-regulated pr... 30 1.2
TC87321 similar to PIR|C86148|C86148 hypothetical protein AAF784... 29 1.6
TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 29 2.1
BQ751379 similar to GP|18376126|em conserved hypothetical protei... 29 2.1
TC76644 similar to GP|6403506|gb|AAF07846.1| putative aminotrans... 28 2.8
BF647088 weakly similar to PIR|D86338|D86 protein F5M15.18 [impo... 28 2.8
BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus... 28 3.6
BQ137157 28 3.6
TC76311 homologue to GP|3204132|emb|CAA07235.1 extensin {Cicer a... 28 3.6
TC81858 similar to GP|18252179|gb|AAL61922.1 unknown protein {Ar... 28 3.6
AW688430 similar to GP|22535581|dbj contains EST AU182189(C63973... 28 3.6
TC77949 weakly similar to GP|11762218|gb|AAG40387.1 AT4g27520 {A... 28 3.6
TC87263 similar to GP|9759298|dbj|BAB09804.1 emb|CAB82953.1~gene... 28 4.7
BF638466 similar to PIR|T07675|T076 cyclin a2-type mitosis-spec... 27 6.1
>TC89082 similar to PIR|H86476|H86476 protein F15O4.35 [imported] -
Arabidopsis thaliana, partial (28%)
Length = 1655
Score = 41.6 bits (96), Expect = 3e-04
Identities = 31/134 (23%), Positives = 66/134 (49%), Gaps = 11/134 (8%)
Frame = +1
Query: 117 IHPQRRQERAS---NNQGRRTMLPSEGTRTS--LPKLPSTQLKSSPSPAKTKASSDHRSL 171
IHP +Q+ +S N+ ++ ++P +G + + + + Q S+ SP+ + +SS S
Sbjct: 208 IHPHHQQQESSSPSNSHHQQLVVPFDGRPSPFIMGSISNIQPSSNSSPSNSSSSSASASA 387
Query: 172 TTTLGKRQSSKTLLPSSQVIRTSKTRSLEKISGNKV------ATDILSVSTPPRQHPEIR 225
+ + ++ L+ +S I T L +S N++ +T + +V+ PP++ + R
Sbjct: 388 SASA----PTQHLIDASLAIATRSEPPLPDVSNNQIQPFSSTSTTVTAVANPPKRSTKDR 555
Query: 226 HSSPGERTRTLCSP 239
H+ R R + P
Sbjct: 556 HTKVDGRGRRIRMP 597
>TC83149 similar to GP|13561982|gb|AAK30594.1 flagelliform silk protein
{Argiope trifasciata}, partial (3%)
Length = 1177
Score = 33.1 bits (74), Expect = 0.11
Identities = 33/122 (27%), Positives = 46/122 (37%)
Frame = +3
Query: 137 PSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSKT 196
PS +RT L P T+ SSP+P+ + SS + +T+ +R SKT LP
Sbjct: 27 PSYASRTPLRSTPPTR-PSSPTPSAPRTSS-RSTPSTSPSRRPRSKTPLPPPP------- 179
Query: 197 RSLEKISGNKVATDILSVSTPPRQHPEIRHSSPGERTRTLCSPCFPTTTSRQEKVEGKDP 256
P QHP+ SP R+ SP P T + P
Sbjct: 180 -------------------PPLPQHPKPPQKSPPRMRRSKPSPSSPAATPLWPQRTTPPP 302
Query: 257 TP 258
+P
Sbjct: 303 SP 308
>TC92828 weakly similar to GP|4115538|dbj|BAA36412.1 UDP-glycose:flavonoid
glycosyltransferase {Vigna mungo}, partial (20%)
Length = 649
Score = 32.7 bits (73), Expect = 0.15
Identities = 35/114 (30%), Positives = 49/114 (42%), Gaps = 6/114 (5%)
Frame = +1
Query: 141 TRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSKTRSLE 200
T + LP LPS+ S PSP LTT K + + S+Q TS S++
Sbjct: 178 TVSQLPTLPSSSTVSLPSP-----------LTTPPPKAEPPPHSISSNQTPSTSNQNSVK 324
Query: 201 --KISGNKVATDILSVSTPPRQHP----EIRHSSPGERTRTLCSPCFPTTTSRQ 248
K +K ++ I SV P +Q P + SS LC P P +T +Q
Sbjct: 325 SPKPLLSKPSSSISSVLPPWKQLPPC*YQFTISSHPVLLYFLCIPTSPKSTRKQ 486
>BQ752265 similar to GP|8101036|gb| delta-1-pyrroline-5-carboxylate
dehydrogenase {Aspergillus nidulans}, partial (48%)
Length = 967
Score = 32.3 bits (72), Expect = 0.19
Identities = 32/110 (29%), Positives = 45/110 (40%), Gaps = 7/110 (6%)
Frame = +2
Query: 143 TSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRT-SKTRSLEK 201
++LP PS PSP T S R T++ ++ LLP S RT + RS +
Sbjct: 89 STLPSPPSPH--GRPSPLPTARPSSSRPPTSSPPSTATTLWLLP*SARARTLGRPRSTRR 262
Query: 202 ISGNKVATDILSV------STPPRQHPEIRHSSPGERTRTLCSPCFPTTT 245
S S S+PP P S ER+R +P P+T+
Sbjct: 263 PSWPTFCASTCSTPRSSTPSSPPTTPPVSGTESSTERSRASSTPSAPSTS 412
>TC92700 weakly similar to PIR|T39956|T39956 probable nadh-dependent flavin
oxidoreductase - fission yeast (Schizosaccharomyces
pombe), partial (10%)
Length = 682
Score = 31.2 bits (69), Expect = 0.43
Identities = 27/85 (31%), Positives = 41/85 (47%), Gaps = 5/85 (5%)
Frame = +3
Query: 119 PQRRQERASNNQGRRTML---PSEGTRTSLPKLPSTQLKSSPSPAKTKA--SSDHRSLTT 173
P RR R+S R + + PS + + P+ PST +SPS ++T A S R T
Sbjct: 3 PSRRCGRSSPPAPRFSSVFPPPSGWSTPASPRGPSTTRSASPSCSRTPAWTCSTSRRAAT 182
Query: 174 TLGKRQSSKTLLPSSQVIRTSKTRS 198
T KR S+ S+ R+++ S
Sbjct: 183 TRRKRLRSRRTTRSASPARSARRSS 257
>TC80088 similar to GP|9759352|dbj|BAB10007.1 gene_id:MAH20.14~unknown
protein {Arabidopsis thaliana}, partial (73%)
Length = 1346
Score = 30.8 bits (68), Expect = 0.56
Identities = 24/71 (33%), Positives = 32/71 (44%), Gaps = 5/71 (7%)
Frame = +1
Query: 138 SEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLL-----PSSQVIR 192
S TS P L ++PSP+ S + + T G +S TLL PS +
Sbjct: 208 SNSVPTSHSLHPVPILLTNPSPSTHSLLSSNATAKTNNGNNNTSNTLLLTNPNPSGKTS* 387
Query: 193 TSKTRSLEKIS 203
T KT S+ KIS
Sbjct: 388 TLKTSSMMKIS 420
>BQ752418 homologue to SP|P78695|GR78 78 kDa glucose-regulated protein
homolog precursor (GRP 78), partial (47%)
Length = 1032
Score = 29.6 bits (65), Expect = 1.2
Identities = 43/187 (22%), Positives = 64/187 (33%), Gaps = 8/187 (4%)
Frame = -2
Query: 4 SSRTFSRPCIASMSL--PSYTKPTTLPRSHAFFARVKKRPLLTVPKSGTDEEAKELT--- 58
SS F P + + L PS PT RS + R L P + ++ T
Sbjct: 554 SSLAFPPPLVVCLKLRSPSSLMPTVFSRSLPTTRALASRSLSPSPTTRAVLPRRKSTAWS 375
Query: 59 ---KAEPSVNNKESSNSVVPGINEEDPPTSKTVSLALIFLGLPRERELLKAHHKRTCHIR 115
++ P +S S + ++ P S+T S R +L A
Sbjct: 374 RRPRSTPRKTRLPASASRLATVSRTTPSPSRTRSTT---------RRVLAARSTTRTRRL 222
Query: 116 YIHPQRRQERASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTL 175
+ P RR G R P RTS + S +PSP + + + T+L
Sbjct: 221 FSRPSRRP-----TTGSRRTAPLPTPRTSRSRRRSCPTSPTPSPPRCTRALVAPAARTSL 57
Query: 176 GKRQSSK 182
SSK
Sbjct: 56 PATTSSK 36
>TC87321 similar to PIR|C86148|C86148 hypothetical protein AAF78401.1
[imported] - Arabidopsis thaliana, partial (70%)
Length = 1413
Score = 29.3 bits (64), Expect = 1.6
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 5/47 (10%)
Frame = -2
Query: 97 LPRERELLKAH-----HKRTCHIRYIHPQRRQERASNNQGRRTMLPS 138
LPRE+ L+AH C + I + E N+G RT+LPS
Sbjct: 269 LPREQHTLRAHSF*Q*QVEKCSVEMI--EEASEAEERNRGTRTLLPS 135
>TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (69%)
Length = 1615
Score = 28.9 bits (63), Expect = 2.1
Identities = 27/88 (30%), Positives = 40/88 (44%)
Frame = -3
Query: 154 KSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSKTRSLEKISGNKVATDILS 213
KSS P ++KA S TT+ S+ T+ PS++ TS T K + + +T S
Sbjct: 1319 KSSLFPFRSKAWFIESSTTTSSSSTTSTATI-PSTKSTSTSSTTPTTKSTASSTSTK--S 1149
Query: 214 VSTPPRQHPEIRHSSPGERTRTLCSPCF 241
+T P S+ RT T+ CF
Sbjct: 1148 TTTTPST------STSTTRTLTISRFCF 1083
>BQ751379 similar to GP|18376126|em conserved hypothetical protein
{Neurospora crassa}, partial (30%)
Length = 771
Score = 28.9 bits (63), Expect = 2.1
Identities = 27/108 (25%), Positives = 47/108 (43%), Gaps = 7/108 (6%)
Frame = +2
Query: 156 SPSPAKTKASSDHRSLTTTLGKRQ---SSKTLLPSSQVIRTSKTRSLEKISGNKVATDIL 212
S +P +++SS R T T R SS PSS + ++ T S+ ++ A +
Sbjct: 29 SSTPCASRSSSSFRISTATTSSRSASTSSAPRTPSSYLTPSAPTASMLVLTATAAAFSSV 208
Query: 213 SVSTPP---RQHPEIRH-SSPGERTRTLCSPCFPTTTSRQEKVEGKDP 256
+ TPP R +R S+P ++T + + +T+S P
Sbjct: 209 ASITPPVTRRSGSLLRSLSTPPSWSKTPLATTWSSTSSTSTNPPSPSP 352
>TC76644 similar to GP|6403506|gb|AAF07846.1| putative aminotransferase
{Arabidopsis thaliana}, partial (89%)
Length = 1918
Score = 28.5 bits (62), Expect = 2.8
Identities = 19/79 (24%), Positives = 35/79 (44%), Gaps = 4/79 (5%)
Frame = +2
Query: 107 HHKRTCH----IRYIHPQRRQERASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKT 162
HH+R + + ++ PQ+++ + ++ R S R +P LP+T S
Sbjct: 308 HHRRRANSSFNVAFLSPQQKEPKTTSPPNHRCFHRSITFRHRIPVLPATTFSRSVGNISV 487
Query: 163 KASSDHRSLTTTLGKRQSS 181
+ S +L T K +SS
Sbjct: 488 RRSCISTNLR*TSWKGRSS 544
>BF647088 weakly similar to PIR|D86338|D86 protein F5M15.18 [imported] -
Arabidopsis thaliana, partial (2%)
Length = 678
Score = 28.5 bits (62), Expect = 2.8
Identities = 27/123 (21%), Positives = 50/123 (39%)
Frame = +3
Query: 138 SEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSKTR 197
S+ T L P + + +P P+ T S+ ++L++ + ++ PSS + S+
Sbjct: 60 SDPHSTFLHHTPISPPQHTPXPSTTLTSTPTQTLSSXTQQHLTNSLTPPSSNATKPSRKT 239
Query: 198 SLEKISGNKVATDILSVSTPPRQHPEIRHSSPGERTRTLCSPCFPTTTSRQEKVEGKDPT 257
SL S N +TP P+ R ++ S C P + ++ + P
Sbjct: 240 SLSXDSPN---------TTPHSFFPQTXF-----RFQSSTSHCSPLASVFHQQTQSPLPQ 377
Query: 258 PFL 260
FL
Sbjct: 378 KFL 386
>BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus
norvegicus}, partial (9%)
Length = 620
Score = 28.1 bits (61), Expect = 3.6
Identities = 43/167 (25%), Positives = 61/167 (35%), Gaps = 2/167 (1%)
Frame = +2
Query: 9 SRPCIASMSLPSYTKPTTLPR--SHAFFARVKKRPLLTVPKSGTDEEAKELTKAEPSVNN 66
SRP S S S + TT PR S A RP +P + T A T+
Sbjct: 107 SRPTSTSSSPSSSRRATTRPRAPSQASPPGPPSRPPSPMPWT*TRRAATAATRT------ 268
Query: 67 KESSNSVVPGINEEDPPTSKTVSLALIFLGLPRERELLKAHHKRTCHIRYIHPQRRQERA 126
++ +V P T T + R R L Y+ P+RR+ R
Sbjct: 269 --AAIAVTAATRTATPATIATTAATRAQTTALRTRSL------------YLRPRRRRRR- 403
Query: 127 SNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTT 173
R PS R+ P P+T +SPS + + + R+ TT
Sbjct: 404 ------RRRRPS---RSPPPATPATTPAASPSLSPSLXTCPSRNQTT 517
>BQ137157
Length = 1053
Score = 28.1 bits (61), Expect = 3.6
Identities = 17/55 (30%), Positives = 23/55 (40%)
Frame = +2
Query: 209 TDILSVSTPPRQHPEIRHSSPGERTRTLCSPCFPTTTSRQEKVEGKDPTPFLNPN 263
T + S S PP +H H ER PT ++ EK + PF+ PN
Sbjct: 143 TPMRSTSIPPPKHTARPHPKLAER---------PTADAKGEKQTKRHHLPFIRPN 280
>TC76311 homologue to GP|3204132|emb|CAA07235.1 extensin {Cicer arietinum},
partial (77%)
Length = 973
Score = 28.1 bits (61), Expect = 3.6
Identities = 34/138 (24%), Positives = 57/138 (40%), Gaps = 19/138 (13%)
Frame = +1
Query: 141 TRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTT-LGKRQSSKTLLPSSQVIRTSKTRSL 199
T + L ++ ST + + + + +S S++TT L +SS T+ SS + +
Sbjct: 67 TTSLLLQITSTTIPVTSTSLLLQVTSTSISISTTSLLLSKSSSTISHSSYSLPLQIPSTT 246
Query: 200 EKISGNKVA------TDILSVSTP----------PRQHPEIRHSSPGERTRTLCSP--CF 241
+S + T I+S + P R H I+ +SP + S CF
Sbjct: 247 NGVSSTSLPLCESPPTYIISTTLPLHITTSSLPSSRSHIHIQITSPTNEITSSASLHLCF 426
Query: 242 PTTTSRQEKVEGKDPTPF 259
PTTT Q E + P+
Sbjct: 427 PTTTYLQVSSEYNNSVPY 480
>TC81858 similar to GP|18252179|gb|AAL61922.1 unknown protein {Arabidopsis
thaliana}, partial (9%)
Length = 748
Score = 28.1 bits (61), Expect = 3.6
Identities = 18/51 (35%), Positives = 24/51 (46%)
Frame = +1
Query: 208 ATDILSVSTPPRQHPEIRHSSPGERTRTLCSPCFPTTTSRQEKVEGKDPTP 258
AT S +TPP+ SSP + + SP PT TS + VE +P
Sbjct: 205 ATPPSSTTTPPKSSATSPTSSPAPK---VSSPPSPTPTSAEAPVESPTESP 348
>AW688430 similar to GP|22535581|dbj contains EST AU182189(C63973)~similar to
Arabidopsis thaliana chromosome5 At5g65910~unknown,
partial (2%)
Length = 674
Score = 28.1 bits (61), Expect = 3.6
Identities = 20/74 (27%), Positives = 37/74 (49%), Gaps = 1/74 (1%)
Frame = +3
Query: 179 QSSKTLLPSSQVIRTSKTR-SLEKISGNKVATDILSVSTPPRQHPEIRHSSPGERTRTLC 237
++++ LL S+ SK R ++ + N ++ +L TPP ++ + LC
Sbjct: 156 ETTRLLLLSNNNNNNSKARRNVSWKNSNLLSPSLLPNLTPP*VFQTLK-----SLMKLLC 320
Query: 238 SPCFPTTTSRQEKV 251
S FP+TT QE++
Sbjct: 321 SIAFPSTTENQEEI 362
>TC77949 weakly similar to GP|11762218|gb|AAG40387.1 AT4g27520 {Arabidopsis
thaliana}, partial (34%)
Length = 1297
Score = 28.1 bits (61), Expect = 3.6
Identities = 28/94 (29%), Positives = 37/94 (38%), Gaps = 4/94 (4%)
Frame = +3
Query: 140 GTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTL----GKRQSSKTLLPSSQVIRTSK 195
G + +L + KSSPSP+ S S TTT G S + P+S + T
Sbjct: 459 GQKLTLVVISPRTPKSSPSPSAGGLSPPSPSPTTTTPSPSGSPPSPVAIPPASSPVPT-- 632
Query: 196 TRSLEKISGNKVATDILSVSTPPRQHPEIRHSSP 229
SG ++ VSTPP P SP
Sbjct: 633 -------SGPTASSPSPVVSTPPAGGPMASSPSP 713
>TC87263 similar to GP|9759298|dbj|BAB09804.1
emb|CAB82953.1~gene_id:MPH15.5~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (65%)
Length = 2229
Score = 27.7 bits (60), Expect = 4.7
Identities = 23/89 (25%), Positives = 35/89 (38%)
Frame = +2
Query: 13 IASMSLPSYTKPTTLPRSHAFFARVKKRPLLTVPKSGTDEEAKELTKAEPSVNNKESSNS 72
I S + S TKPT+ VKK+P L + +E K + + + N
Sbjct: 530 IPSKNNTSITKPTS--------QIVKKQPSLVTNHTSNSDETKVMKNSNQTTNATVEGVP 685
Query: 73 VVPGINEEDPPTSKTVSLALIFLGLPRER 101
+VP N + K V L L R++
Sbjct: 686 IVPNKNLSSDSSLKGVDLHNYTASLARKQ 772
>BF638466 similar to PIR|T07675|T076 cyclin a2-type mitosis-specific -
soybean, partial (9%)
Length = 566
Score = 27.3 bits (59), Expect = 6.1
Identities = 24/95 (25%), Positives = 41/95 (42%), Gaps = 1/95 (1%)
Frame = +3
Query: 125 RASNNQGRRTMLPSEGTRTSLPKLPS-TQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKT 183
+ SN RR+ S T + + P T L S + K A+S + T Q+S
Sbjct: 153 KMSNXNNRRSSFSSSTTSSMAKRXPPVTXLTSENNAGKVLAASAKKRPPLTNLTNQNSSR 332
Query: 184 LLPSSQVIRTSKTRSLEKISGNKVATDILSVSTPP 218
S+ V + +KT+ E N ++ ++ V+ P
Sbjct: 333 NNSSTLVTKVAKTKK-EPSPCNSSSSSVIXVNKKP 434
Score = 27.3 bits (59), Expect = 6.1
Identities = 21/81 (25%), Positives = 35/81 (42%)
Frame = +3
Query: 4 SSRTFSRPCIASMSLPSYTKPTTLPRSHAFFARVKKRPLLTVPKSGTDEEAKELTKAEPS 63
SSR S + ++ + +P+ S + V K+P L+ KS T K +T +
Sbjct: 324 SSRNNSSTLVTKVA-KTKKEPSPCNSSSSSVIXVNKKPTLSNVKSATVVFPKAITTTTXT 500
Query: 64 VNNKESSNSVVPGINEEDPPT 84
++ N VVP + P T
Sbjct: 501 TSSFSGKNEVVPPXSSGAPTT 563
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.125 0.354
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,264,649
Number of Sequences: 36976
Number of extensions: 113955
Number of successful extensions: 848
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 848
length of query: 263
length of database: 9,014,727
effective HSP length: 94
effective length of query: 169
effective length of database: 5,538,983
effective search space: 936088127
effective search space used: 936088127
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0012a.1