BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0011b.6
(189 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93101 57 6e-09
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 55 2e-08
BG586862 51 3e-07
BG456581 43 6e-05
BG453645 35 0.023
BF631765 33 0.051
BG449067 32 0.19
BG648572 weakly similar to GP|9802748|gb Unknown protein {Arabid... 29 1.2
AL382240 29 1.2
BF634872 28 1.6
BQ751198 homologue to GP|12718253|em probable importin alpha SRP... 28 2.1
CB892842 similar to PIR|H96695|H966 hypothetical protein F5A8.10... 28 2.8
TC82275 similar to PIR|H96695|H96695 hypothetical protein F5A8.1... 28 2.8
CA922772 weakly similar to GP|7211993|gb|A ESTs gb|T76367 and gb... 28 2.8
BQ155506 similar to GP|15809917|gb AT4g00030/F6N15_13 {Arabidops... 27 3.6
TC93136 27 4.7
TC85883 apyrase-like protein [Medicago truncatula] 27 4.7
TC81529 similar to GP|22335711|dbj|BAC10553. ABA insensitive 3 {... 27 6.2
BG648345 similar to PIR|T04746|T047 hypothetical protein T16H5.1... 26 8.1
AJ503183 similar to GP|10177176|db SMC-like protein {Arabidopsis... 26 8.1
>TC93101
Length = 675
Score = 56.6 bits (135), Expect = 6e-09
Identities = 42/183 (22%), Positives = 74/183 (39%), Gaps = 21/183 (11%)
Frame = -1
Query: 1 MRSALIQRKVEVDPGCAICGNEVETMEHLFLEFPMFLGVWFASSLSLRV--NLFATVEDF 58
+R L +R V P C C +ET H+F+ VWF S LS+R N D+
Sbjct: 657 VRXELNKRGVNCPPLCPRCYFNLETTNHIFMSCERTQRVWFGSQLSIRFPDNSTINFSDW 478
Query: 59 VSEMVREGMPEVLAAVQTMLYAIWESQNGLVFQRMSFDMGWVLAWA-------------- 104
+ + + E++ + + Y+IW ++N +F+ ++ A
Sbjct: 477 LFDAISNQTEEIIIKISAITYSIWHARNKAIFENQFVSEDTIIQ*AQNSILAYEQATIKP 298
Query: 105 ----VALGSGWVRVHESPHAGR-ELPSRWKRPATRAVKVNLDARVHDGSMAGLVLVARDE 159
+ L S + + R + SRW++P +K N DA + GL + R+
Sbjct: 297 QNPNIVLSSLSATSNTNTTRRRSNVRSRWQKPLNNILKANCDANLQVQGRWGLGCIIRNA 118
Query: 160 NEE 162
+ E
Sbjct: 117 DGE 109
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 54.7 bits (130), Expect = 2e-08
Identities = 45/184 (24%), Positives = 85/184 (45%), Gaps = 16/184 (8%)
Frame = +3
Query: 1 MRSALIQRKVEVDPGCAICGNEVETMEHLFLEFPMFLGVWFASSLSLRVNLFATVEDFVS 60
+RS+L +R ++ P C C ++ ET+ HLF+ P+ VWF S+L + + +F++
Sbjct: 198 VRSSLRKRGIQCYPLCPRCHSKTETITHLFMSCPLSKRVWFGSNLCINFDNLPN-PNFIN 374
Query: 61 ---EMVREGMPEVLAAVQTMLYAIWESQNGLVFQRMSFDMGWVLAWAVALGSGWVRVHES 117
E + + + ++Y +W ++N V + + ++ A S + + +
Sbjct: 375 *LYEAIL*KDECITI*IAAIIYNLWHARNLSVLEDQTILEMDIIQRASNCISDYKQANTQ 554
Query: 118 --PHAGR-----------ELPSRWKRPATRAVKVNLDARVHDGSMAGLVLVARDENEEVL 164
P R ++WKRP VKVN DA + + GL ++ RDE V+
Sbjct: 555 APPSMARTGYDPRSQHRPAKNTKWKRPNLGLVKVNTDANLQNHGKWGLGIIIRDEVGLVM 734
Query: 165 EATS 168
A++
Sbjct: 735 AAST 746
>BG586862
Length = 804
Score = 50.8 bits (120), Expect = 3e-07
Identities = 24/95 (25%), Positives = 50/95 (52%), Gaps = 2/95 (2%)
Frame = -1
Query: 1 MRSALIQRKVEVDPGCAICGNEVETMEHLFLEFPMFLGVWFASSLSLRVNLFATV--EDF 58
++ L +R + C C +++ET++HLFL + WF S L + + + D+
Sbjct: 585 VKDELHKRGIRCSLLCPRCESKIETVQHLFLNCEVTQKEWFGSQLGINFHSSGVLHFHDW 406
Query: 59 VSEMVREGMPEVLAAVQTMLYAIWESQNGLVFQRM 93
++ + + E + A+ +LY+IW ++N VF+ +
Sbjct: 405 ITNFILKNDEETIIALTALLYSIWHARNQKVFENI 301
>BG456581
Length = 683
Score = 43.1 bits (100), Expect = 6e-05
Identities = 26/80 (32%), Positives = 44/80 (54%), Gaps = 5/80 (6%)
Frame = +2
Query: 16 CAICGNEVETMEHLFLEFPMFLGVWFASSLSLRVNL-FATVEDFVSEMVREGMPEV---- 70
C+ C +VET +HLFL + +W LRV L F++ + +S + R +V
Sbjct: 194 CSFCLEQVETSDHLFLRCKFVVTLWSWLCSQLRVGLDFSSFKALLSSLPRHCSSQVRDLY 373
Query: 71 LAAVQTMLYAIWESQNGLVF 90
+AAV M+++IW ++N + F
Sbjct: 374 VAAVVHMVHSIWWARNNVRF 433
>BG453645
Length = 622
Score = 34.7 bits (78), Expect = 0.023
Identities = 25/91 (27%), Positives = 37/91 (40%), Gaps = 7/91 (7%)
Frame = +3
Query: 11 EVDPGCAICGNEVETMEHLFLEFPMFLGVWFASSLSLRVNLFATVEDFVS-EMVREGMPE 69
EV C +C +VET H+FL + VWF VN FV E EG
Sbjct: 18 EVSSTCVLCNLKVETTTHIFLHCDVASLVWFRVMKWFDVNFLIPPNLFVHWECWSEGGSA 197
Query: 70 VLAAV------QTMLYAIWESQNGLVFQRMS 94
T ++ +W +N L+F+ ++
Sbjct: 198 NRVTKGHWLVWHTTIWVLWAKRNDLIFKGLN 290
>BF631765
Length = 498
Score = 33.5 bits (75), Expect = 0.051
Identities = 30/111 (27%), Positives = 45/111 (40%)
Frame = +1
Query: 76 TMLYAIWESQNGLVFQRMSFDMGWVLAWAVALGSGWVRVHESPHAGRELPSRWKRPATRA 135
T L+ IW+ +N VF + FD V A + + + R + P +
Sbjct: 124 TSLWLIWKHRNQKVFNNVDFDPPLVSALVEEFSIANLPLKRGQVC---VVPR*QPPPFGS 294
Query: 136 VKVNLDARVHDGSMAGLVLVARDENEEVLEATSSYPWPILSPLLAEALSLR 186
+KVN+ A G +AR+ L A + SPLLAE L L+
Sbjct: 295 IKVNVGAGCFGDGSTGWGFIARNAAGLALSAETKSDGISYSPLLAECLLLK 447
>BG449067
Length = 578
Score = 31.6 bits (70), Expect = 0.19
Identities = 16/63 (25%), Positives = 33/63 (51%)
Frame = -2
Query: 127 RWKRPATRAVKVNLDARVHDGSMAGLVLVARDENEEVLEATSSYPWPILSPLLAEALSLR 186
+WK+P +K+N DA + + G+ +VAR++ + + + + + S AEA +
Sbjct: 310 KWKKPEKDIIKLNSDANLSSTDLWGIGVVARNDEGFAMASGTWFRFGFPSATTAEAWGIY 131
Query: 187 WSM 189
+M
Sbjct: 130 QAM 122
>BG648572 weakly similar to GP|9802748|gb Unknown protein {Arabidopsis
thaliana}, partial (12%)
Length = 697
Score = 28.9 bits (63), Expect = 1.2
Identities = 25/71 (35%), Positives = 38/71 (53%), Gaps = 14/71 (19%)
Frame = +2
Query: 33 FPMFLGVWFA-SSLSLRVNLFAT-VEDFVS------EMVREG------MPEVLAAVQTML 78
FP L W A +SL+LR N F+ + DF+S E+ +G +P+ + +Q +L
Sbjct: 476 FPSSLRSWTALTSLTLRENRFSGGIPDFLSAFENLNELKLDGNKFWRNIPKSIEQLQNLL 655
Query: 79 YAIWESQNGLV 89
Y + S NGLV
Sbjct: 656 YDLNLSANGLV 688
>AL382240
Length = 320
Score = 28.9 bits (63), Expect = 1.2
Identities = 15/48 (31%), Positives = 26/48 (53%), Gaps = 1/48 (2%)
Frame = +3
Query: 128 WKRPATRAVKVNLDARVHDG-SMAGLVLVARDENEEVLEATSSYPWPI 174
WK+PA K N+DA ++ GL + RDE++ + A + + P+
Sbjct: 129 WKKPAPGXYKCNIDASFSTSFNIVGLGMCLRDEDDAFVLARTEWFAPL 272
>BF634872
Length = 688
Score = 28.5 bits (62), Expect = 1.6
Identities = 15/40 (37%), Positives = 26/40 (64%), Gaps = 2/40 (5%)
Frame = +1
Query: 40 WFASSL--SLRVNLFATVEDFVSEMVREGMPEVLAAVQTM 77
+F SL + + N+ + VED VS+ +REG+P + +QT+
Sbjct: 217 YFCCSLVDAFKGNIGSAVEDAVSKKIREGIPTLDNLLQTL 336
>BQ751198 homologue to GP|12718253|em probable importin alpha SRP1
{Neurospora crassa}, partial (35%)
Length = 813
Score = 28.1 bits (61), Expect = 2.1
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = +1
Query: 157 RDENEEVLEATSSYPWPILSPLLAEALSLRWS 188
R ++ VL +SS W +LSP+ A LS W+
Sbjct: 706 RSSSKPVLFPSSSSFWAVLSPMCASRLSGHWA 801
>CB892842 similar to PIR|H96695|H966 hypothetical protein F5A8.10 [imported]
- Arabidopsis thaliana, partial (23%)
Length = 846
Score = 27.7 bits (60), Expect = 2.8
Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -1
Query: 116 ESPHAGRELPSRWKRPATR-AVKVNLDARVHDGSM 149
+ P R LPS+WK ATR + K ++ ++ DGS+
Sbjct: 828 QKPRIHRHLPSKWKVTATRMSEKWSV*SKSRDGSL 724
>TC82275 similar to PIR|H96695|H96695 hypothetical protein F5A8.10
[imported] - Arabidopsis thaliana, partial (49%)
Length = 751
Score = 27.7 bits (60), Expect = 2.8
Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -1
Query: 116 ESPHAGRELPSRWKRPATR-AVKVNLDARVHDGSM 149
+ P R LPS+WK ATR + K ++ ++ DGS+
Sbjct: 400 QKPRIHRHLPSKWKVTATRMSEKWSV*SKSRDGSL 296
>CA922772 weakly similar to GP|7211993|gb|A ESTs gb|T76367 and gb|AA404955
come from this gene. {Arabidopsis thaliana}, partial
(16%)
Length = 804
Score = 27.7 bits (60), Expect = 2.8
Identities = 12/28 (42%), Positives = 20/28 (70%)
Frame = +2
Query: 50 NLFATVEDFVSEMVREGMPEVLAAVQTM 77
N+ + VED VS+ +REG+P + +QT+
Sbjct: 536 NIGSAVEDAVSKKIREGIPTLDNLLQTL 619
>BQ155506 similar to GP|15809917|gb AT4g00030/F6N15_13 {Arabidopsis
thaliana}, partial (60%)
Length = 622
Score = 27.3 bits (59), Expect = 3.6
Identities = 17/59 (28%), Positives = 26/59 (43%)
Frame = +1
Query: 45 LSLRVNLFATVEDFVSEMVREGMPEVLAAVQTMLYAIWESQNGLVFQRMSFDMGWVLAW 103
L+ + NL + D + + P LA++ T + A+ E GLV S W L W
Sbjct: 46 LTAKKNLLTLISDQDRGLKTQKNPTKLASIVTAIDAMAELGTGLVTTGDSLSATWRLLW 222
>TC93136
Length = 722
Score = 26.9 bits (58), Expect = 4.7
Identities = 19/71 (26%), Positives = 27/71 (37%)
Frame = +1
Query: 24 ETMEHLFLEFPMFLGVWFASSLSLRVNLFATVEDFVSEMVREGMPEVLAAVQTMLYAIWE 83
ET HLFL P VW L + F V + R V ++ IW+
Sbjct: 262 ETSSHLFLHCPFLSSVWSKILGWLYYSKFVCVFRLLER--RSRTKGVWLIWHATIWVIWK 435
Query: 84 SQNGLVFQRMS 94
N +F+ +S
Sbjct: 436 GINNRIFKNIS 468
>TC85883 apyrase-like protein [Medicago truncatula]
Length = 1848
Score = 26.9 bits (58), Expect = 4.7
Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
Frame = +3
Query: 132 ATRAVKVNLDAR--VHDGSMAGLVLVARDENEEVLEATSS 169
A R V VNL + V G+ AGL L+ + +E +LEA SS
Sbjct: 561 AERVVPVNLQPKTPVKLGATAGLRLLDGNSSELILEAVSS 680
>TC81529 similar to GP|22335711|dbj|BAC10553. ABA insensitive 3 {Pisum
sativum}, partial (37%)
Length = 1075
Score = 26.6 bits (57), Expect = 6.2
Identities = 14/40 (35%), Positives = 19/40 (47%), Gaps = 1/40 (2%)
Frame = -2
Query: 30 FLEFPMFLGVWFASSL-SLRVNLFATVEDFVSEMVREGMP 68
F +FP F WF S + SL + L + F SE + P
Sbjct: 912 FCDFPAFFAFWFCSLVASLYLPLIINLPHFTSEYITMKSP 793
>BG648345 similar to PIR|T04746|T047 hypothetical protein T16H5.10 -
Arabidopsis thaliana, partial (4%)
Length = 788
Score = 26.2 bits (56), Expect = 8.1
Identities = 14/37 (37%), Positives = 20/37 (53%)
Frame = +3
Query: 20 GNEVETMEHLFLEFPMFLGVWFASSLSLRVNLFATVE 56
G V + LFLEFP L V F ++L + + L +E
Sbjct: 378 GLSVNQIALLFLEFPQILMVKFFANLRVCLQLLTEIE 488
>AJ503183 similar to GP|10177176|db SMC-like protein {Arabidopsis thaliana},
partial (6%)
Length = 538
Score = 26.2 bits (56), Expect = 8.1
Identities = 8/21 (38%), Positives = 15/21 (71%)
Frame = -1
Query: 24 ETMEHLFLEFPMFLGVWFASS 44
+++E LF+ FP+F W+ +S
Sbjct: 367 KSLERLFISFPVFFFFWYCTS 305
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.134 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,350,614
Number of Sequences: 36976
Number of extensions: 80247
Number of successful extensions: 382
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 377
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 379
length of query: 189
length of database: 9,014,727
effective HSP length: 91
effective length of query: 98
effective length of database: 5,649,911
effective search space: 553691278
effective search space used: 553691278
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0011b.6