BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.3
(172 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79476 similar to GP|14423560|gb|AAK62462.1 Unknown protein {Ar... 29 1.1
TC77450 similar to PIR|D86188|D86188 hypothetical protein [impor... 28 1.8
BE124649 homologue to PIR|S22489|S22 IgE-dependent histamine-rel... 27 4.1
BQ144401 27 4.1
BF645207 27 4.1
TC89430 similar to SP|P46573|APKB_ARATH Protein kinase APK1B (EC... 27 4.1
BQ145093 homologue to PIR|S22489|S22 IgE-dependent histamine-rel... 27 4.1
TC85136 homologue to SP|P28014|TCTP_MEDSA Translationally contro... 27 4.1
TC86492 homologue to GP|11907554|dbj|BAB19676. expansin {Prunus ... 27 4.1
BM814004 homologue to SP|P28014|TCTP_ Translationally controlled... 27 4.1
TC92738 weakly similar to GP|8777295|dbj|BAA96885.1 cytochrome P... 27 5.3
TC87594 similar to GP|16648787|gb|AAL25584.1 At1g26270/F28B23_7 ... 27 5.3
AW688151 similar to PIR|T00606|T006 hypothetical protein At2g025... 27 5.3
BF646244 GP|4104812|gb peripheral benzodiazepine receptor intera... 27 5.3
TC78244 similar to PIR|T00606|T00606 hypothetical protein At2g02... 27 5.3
BQ751450 similar to GP|21954534|db 6-phosphogluconate dehydrogen... 26 6.9
TC76827 similar to GP|17154773|gb|AAL35979.1 extensin-like prote... 26 6.9
TC87438 weakly similar to GP|5734618|dbj|BAA83349.1 Similar to A... 26 6.9
TC88708 26 9.1
>TC79476 similar to GP|14423560|gb|AAK62462.1 Unknown protein {Arabidopsis
thaliana}, partial (43%)
Length = 956
Score = 28.9 bits (63), Expect = 1.1
Identities = 25/81 (30%), Positives = 40/81 (48%), Gaps = 1/81 (1%)
Frame = +3
Query: 15 HSTLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSST-LTPNNPSGEALEVRFCTVL 73
+S V EEEEEE K +LPE + +ST L P+ G +L R +
Sbjct: 66 NSVHVEHEEEEEEETNNLKHRNTKPVKQTLPEVLNRLASTILFPDPADGTSLLRRIKNSV 245
Query: 74 PNLLHAYVVPSSSRNNDREVI 94
+ +A ++P +SRN+ +V+
Sbjct: 246 AD--NAPLIPEASRNSANDVL 302
>TC77450 similar to PIR|D86188|D86188 hypothetical protein [imported] -
Arabidopsis thaliana, partial (79%)
Length = 1645
Score = 28.1 bits (61), Expect = 1.8
Identities = 22/94 (23%), Positives = 43/94 (45%)
Frame = +1
Query: 4 KEKELITFTFPHSTLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGE 63
+ K+L+ FTF HST V ++ + + K N+ ER +SS T +P
Sbjct: 7 RNKKLVQFTFQHSTTVSIKH----------LVLLKFNI----ERWRSSSMT*SP------ 126
Query: 64 ALEVRFCTVLPNLLHAYVVPSSSRNNDREVIAVV 97
+ ++ P +H + +PS N+ ++ ++
Sbjct: 127---IFNLSINPFQIHLFAMPSIRFNHTLNILLIL 219
>BE124649 homologue to PIR|S22489|S22 IgE-dependent histamine-releasing
factor homolog - alfalfa (fragment), complete
Length = 608
Score = 26.9 bits (58), Expect = 4.1
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = -3
Query: 40 NLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAYVVPSSSRN 88
N S++ + A SST + + PS EAL + P H PS+S N
Sbjct: 282 NTSTMSTTLTAESSTPSSSPPSAEALAPMSTSTTPLTTH---FPSTSHN 145
>BQ144401
Length = 1107
Score = 26.9 bits (58), Expect = 4.1
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +3
Query: 107 FPGVFFLGKPSAVLPVLADIL 127
FP FF+GK VLP AD L
Sbjct: 24 FPKFFFMGKKVGVLPEFADAL 86
>BF645207
Length = 627
Score = 26.9 bits (58), Expect = 4.1
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = -2
Query: 2 KVKEKELITFTFPHSTLVRVREEE 25
K EL+T FPH LVRV +++
Sbjct: 212 KTTTPELMTVAFPHQNLVRVSQDK 141
>TC89430 similar to SP|P46573|APKB_ARATH Protein kinase APK1B (EC 2.7.1.-).
[Mouse-ear cress] {Arabidopsis thaliana}, partial (71%)
Length = 1663
Score = 26.9 bits (58), Expect = 4.1
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +3
Query: 68 RFCTVLPNLLHAYVVPSSSR 87
R VLPN+LH++ VP +R
Sbjct: 774 RLLLVLPNVLHSFTVPKQNR 833
>BQ145093 homologue to PIR|S22489|S22 IgE-dependent histamine-releasing
factor homolog - alfalfa (fragment), partial (89%)
Length = 671
Score = 26.9 bits (58), Expect = 4.1
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = -1
Query: 40 NLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAYVVPSSSRN 88
N S++ + A SST + + PS EAL + P H PS+S N
Sbjct: 146 NTSTMSTTLTAESSTPSSSPPSAEALAPMSTSTTPLTTH---FPSTSHN 9
>TC85136 homologue to SP|P28014|TCTP_MEDSA Translationally controlled tumor
protein homolog (TCTP). [Alfalfa] {Medicago sativa},
complete
Length = 889
Score = 26.9 bits (58), Expect = 4.1
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = -3
Query: 40 NLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAYVVPSSSRN 88
N S++ + A SST + + PS EAL + P H PS+S N
Sbjct: 302 NTSTMSTTLTAESSTPSSSPPSAEALAPMSTSTTPLTTH---FPSTSHN 165
>TC86492 homologue to GP|11907554|dbj|BAB19676. expansin {Prunus persica},
partial (95%)
Length = 1257
Score = 26.9 bits (58), Expect = 4.1
Identities = 27/94 (28%), Positives = 45/94 (47%), Gaps = 16/94 (17%)
Frame = -2
Query: 8 LITFTFPHSTLVRVREEEEE---------EEEAYFVSMAKAN-LSSLP----ERIAASSS 53
LI +FPH+ ++ R++ E E F+ ++ LS LP +A+ SS
Sbjct: 1046 LIFLSFPHTFQLKQRKQCNAIYYTSFF*LEIELMFLKLSPCKCLSKLPIARHNVVASDSS 867
Query: 54 TLTPNNPSGEALEVRFCTVLPNL--LHAYVVPSS 85
T+T ++ EAL + LP L + + +PSS
Sbjct: 866 TITCSDLE*EALTIEIVIALPVLPPVPRHSLPSS 765
>BM814004 homologue to SP|P28014|TCTP_ Translationally controlled tumor
protein homolog (TCTP). [Alfalfa] {Medicago sativa},
partial (58%)
Length = 460
Score = 26.9 bits (58), Expect = 4.1
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = -1
Query: 40 NLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAYVVPSSSRN 88
N S++ + A SST + + PS EAL + P H PS+S N
Sbjct: 271 NTSTMSTTLTAESSTPSSSPPSAEALAPMSTSTTPLTTH---FPSTSHN 134
>TC92738 weakly similar to GP|8777295|dbj|BAA96885.1 cytochrome P450-like
{Arabidopsis thaliana}, partial (14%)
Length = 756
Score = 26.6 bits (57), Expect = 5.3
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 30 EAYFVSMAKANLSSLPERIAASSSTLTPNN 59
E+YF ++ NL P R A +S L+P N
Sbjct: 333 ESYFFILSCMNLICFPSRFAFRNSKLSPTN 244
>TC87594 similar to GP|16648787|gb|AAL25584.1 At1g26270/F28B23_7
{Arabidopsis thaliana}, partial (40%)
Length = 1522
Score = 26.6 bits (57), Expect = 5.3
Identities = 16/30 (53%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = +1
Query: 21 VREEEEEEEEAYFVSMAK--ANLSSLPERI 48
V EEEEEE +AY SM K N+S L E +
Sbjct: 967 VIEEEEEECDAYSNSMGKLVLNISKLSESV 1056
>AW688151 similar to PIR|T00606|T006 hypothetical protein At2g02570
[imported] - Arabidopsis thaliana, partial (5%)
Length = 534
Score = 26.6 bits (57), Expect = 5.3
Identities = 14/48 (29%), Positives = 24/48 (49%)
Frame = +2
Query: 20 RVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEV 67
R EEEEEEEE + +NLS+ +++ L + E +++
Sbjct: 44 REEEEEEEEEEELNIGELASNLSTYKQQLHQVRELLNDEPTNSEYVDM 187
>BF646244 GP|4104812|gb peripheral benzodiazepine receptor interacting
protein; PBR-IP/PRAX1 {Homo sapiens}, partial (0%)
Length = 643
Score = 26.6 bits (57), Expect = 5.3
Identities = 14/48 (29%), Positives = 24/48 (49%)
Frame = +2
Query: 20 RVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEV 67
R EEEEEEEE + +NLS+ +++ L + E +++
Sbjct: 38 REEEEEEEEEEELNIGELASNLSTYKQQLHQVRELLNDEPTNSEYVDM 181
>TC78244 similar to PIR|T00606|T00606 hypothetical protein At2g02570
[imported] - Arabidopsis thaliana, partial (71%)
Length = 1166
Score = 26.6 bits (57), Expect = 5.3
Identities = 14/48 (29%), Positives = 24/48 (49%)
Frame = +3
Query: 20 RVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEV 67
R EEEEEEEE + +NLS+ +++ L + E +++
Sbjct: 66 REEEEEEEEEEELNIGELASNLSTYKQQLHQVRELLNDEPTNSEYVDM 209
>BQ751450 similar to GP|21954534|db 6-phosphogluconate dehydrogenase
{Aspergillus oryzae}, partial (36%)
Length = 661
Score = 26.2 bits (56), Expect = 6.9
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +3
Query: 24 EEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAY 80
EE EEE V + ++ + E A + TP+ +G +E+ +L +LLH +
Sbjct: 396 EEVVEEEVLEVGVGAVSVGDVLEEDGADDAATTPHEGNGGLVELP-AVLLGSLLHEH 563
>TC76827 similar to GP|17154773|gb|AAL35979.1 extensin-like protein {Cucumis
sativus}, partial (60%)
Length = 1093
Score = 26.2 bits (56), Expect = 6.9
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +2
Query: 37 AKANLSSLPERIAASSSTLTPNNPSGEALEVRF 69
+ ++ S P A SSS TP+NPS A F
Sbjct: 290 SSSSTPSNPSNFAYSSSPSTPSNPSNFAYSTNF 388
>TC87438 weakly similar to GP|5734618|dbj|BAA83349.1 Similar to Arabidopsis
thaliana chromosome II BAC F5H14 genomic sequence;
unknown protein (AC006234), partial (63%)
Length = 1358
Score = 26.2 bits (56), Expect = 6.9
Identities = 17/46 (36%), Positives = 24/46 (51%)
Frame = -2
Query: 16 STLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPS 61
S LVR+ ++ +YF + N L R AA++ST P NPS
Sbjct: 898 SNLVRL-----QQHASYFSGILTNNPERLHRRKAAATSTNLPCNPS 776
>TC88708
Length = 478
Score = 25.8 bits (55), Expect = 9.1
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 20 RVREEEEEEEEAYFVSMAKANLSS 43
R EEEEEEEE F+S + S
Sbjct: 41 REEEEEEEEEEEEFISCVSSTAPS 112
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.135 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,294,939
Number of Sequences: 36976
Number of extensions: 74751
Number of successful extensions: 690
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 598
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 652
length of query: 172
length of database: 9,014,727
effective HSP length: 89
effective length of query: 83
effective length of database: 5,723,863
effective search space: 475080629
effective search space used: 475080629
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0010.3