BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.21
(161 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89446 similar to GP|21592451|gb|AAM64402.1 unknown {Arabidopsi... 256 3e-69
BQ144139 119 4e-28
TC88324 similar to PIR|F86238|F86238 protein T10O24.11 [imported... 76 5e-15
TC78318 similar to PIR|T45852|T45852 hypothetical protein F3A4.7... 31 0.25
TC84544 similar to GP|13591696|gb|AAK31318.1 histone acetyltrans... 31 0.25
AW690594 similar to GP|23498163|emb hypothetical protein {Plasmo... 31 0.25
TC82350 similar to GP|9837385|gb|AAG00554.1| retinitis pigmentos... 30 0.43
TC84839 similar to GP|19386827|dbj|BAB86205. hypothetical protei... 30 0.43
BF641220 weakly similar to PIR|G86203|G86 probable N-arginine di... 30 0.56
TC78385 weakly similar to PIR|T21861|T21861 hypothetical protein... 30 0.56
TC78386 similar to SP|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprote... 30 0.56
TC86045 similar to PIR|T46225|T46225 alpha NAC-like protein - Ar... 29 0.73
TC87242 homologue to PIR|S56716|S56716 protein kinase SPK-3 (EC ... 29 0.73
TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding... 29 0.95
TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Atel... 28 1.2
TC84341 homologue to GP|48188|emb|CAA77863.1|| N-terminus of bet... 28 1.2
AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapi... 28 1.2
TC87729 similar to GP|9759461|dbj|BAB10377.1 gene_id:MAF19.15~un... 28 1.6
TC83341 weakly similar to GP|8978076|dbj|BAA98104.1 gene_id:K14A... 28 1.6
TC89370 weakly similar to PIR|T01856|T01856 hypothetical protein... 28 1.6
>TC89446 similar to GP|21592451|gb|AAM64402.1 unknown {Arabidopsis
thaliana}, partial (54%)
Length = 747
Score = 256 bits (654), Expect = 3e-69
Identities = 135/162 (83%), Positives = 149/162 (91%), Gaps = 1/162 (0%)
Frame = +3
Query: 1 MQTSSLFRRLAPSLAARFRQNQKL-TSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCL 59
MQTS LFRRLAP LAARF++NQ + +SFSSSSSSSVLH AT SS SS VEPVH+TENC+
Sbjct: 45 MQTS-LFRRLAPLLAARFQRNQNIISSFSSSSSSSVLHHAT-SSPSSPDVEPVHITENCV 218
Query: 60 RRMKELEASESATAPKTLRLSVETGGCSGFQYAFDLDDRINSDDRVFEREGIKLVVDNIS 119
R+MKEL+A+ES++ K LRLSVETGGCSGFQYAF+LDDR NSDDRVFE+EGIKLVVDNIS
Sbjct: 219 RKMKELDANESSSGGKMLRLSVETGGCSGFQYAFNLDDRFNSDDRVFEKEGIKLVVDNIS 398
Query: 120 YDFVKGATVDYVEELIRSAFVVTENPSAVGGCSCKSSFMVKQ 161
YDFVKGATVDYVEELIRSAF+VTENPSAVGGCSCKSSFMVKQ
Sbjct: 399 YDFVKGATVDYVEELIRSAFIVTENPSAVGGCSCKSSFMVKQ 524
>BQ144139
Length = 685
Score = 119 bits (299), Expect = 4e-28
Identities = 79/167 (47%), Positives = 104/167 (61%), Gaps = 8/167 (4%)
Frame = +3
Query: 3 TSSLFRRLAPSL-AARFRQNQKLT-SFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLR 60
TSSL R+ + R ++ Q + S SSSSSS+L+ A+ S VEP H+T+NC R
Sbjct: 51 TSSLIRKTSLHYWHHRLQRTQNIIISI*SSSSSSLLYHASYSPDCPD-VEPAHITQNCAR 227
Query: 61 RMKELEASESATAPKTLRLSVETGGCSGFQYAFDLDDRINSDDRVFEREGIKLVVDN-IS 119
MKEL A+E ++ K L V TGGCSGFQ A++ DDR NSDD++F++EGIKLVVDN +
Sbjct: 228 IMKELYANE*SSGGKMLL*IV*TGGCSGFQDAYNSDDRYNSDDKIFDKEGIKLVVDNMVI 407
Query: 120 YDFVKGATVDYVEELIRSA-----FVVTENPSAVGGCSCKSSFMVKQ 161
KG+T E L R A T+NPSA+GGC+ + MVKQ
Sbjct: 408 QPLAKGST----ERLCRGANPCRLSYATDNPSAIGGCN*EKPLMVKQ 536
>TC88324 similar to PIR|F86238|F86238 protein T10O24.11 [imported] -
Arabidopsis thaliana, partial (95%)
Length = 815
Score = 76.3 bits (186), Expect = 5e-15
Identities = 54/157 (34%), Positives = 77/157 (48%), Gaps = 2/157 (1%)
Frame = +1
Query: 3 TSSLFRRLAPSLAARFRQNQKLTSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRM 62
TS FR P L RF + K S S S S A+ + +S + + +T+N L +
Sbjct: 130 TSLSFRSSTPILNRRF--SPKPLSIRSLSVS-----ASAAPASGAVAPAISLTDNALNHL 288
Query: 63 KELEASESATAPKTLRLSVETGGCSGFQYAFDLDDRINS--DDRVFEREGIKLVVDNISY 120
++ + S LR+ V+ GGCSG Y D +DR N+ DD + E +G +V D S
Sbjct: 289 NKMRSERSQDL--CLRIGVKQGGCSGMSYTMDFEDRANARPDDSIIEYKGFVIVCDPKSL 462
Query: 121 DFVKGATVDYVEELIRSAFVVTENPSAVGGCSCKSSF 157
FV G +DY + LI F +NP+A C C SF
Sbjct: 463 LFVFGMQLDYSDALIGGGFNF-KNPNATQTCGCGKSF 570
>TC78318 similar to PIR|T45852|T45852 hypothetical protein F3A4.70 -
Arabidopsis thaliana, partial (48%)
Length = 1923
Score = 30.8 bits (68), Expect = 0.25
Identities = 26/72 (36%), Positives = 35/72 (48%)
Frame = -2
Query: 5 SLFRRLAPSLAARFRQNQKLTSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRMKE 64
+LF+ L + +F + TS SSS+SS L T SSS SS EP R
Sbjct: 740 ALFKVLFIDSSVKFIPLRTCTSSSSSTSSQRLALTTKSSSKSSTSEP--------NRDAS 585
Query: 65 LEASESATAPKT 76
L +S +AT+ T
Sbjct: 584 LSSSNAATSGST 549
>TC84544 similar to GP|13591696|gb|AAK31318.1 histone acetyltransferase GCN5
{Arabidopsis thaliana}, partial (30%)
Length = 816
Score = 30.8 bits (68), Expect = 0.25
Identities = 23/62 (37%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
Frame = -2
Query: 15 AARFRQNQKLT-SFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRMKELEASESATA 73
A +FR + L+ S+SSSSSS+ T SS SSS E + E R +++A +A
Sbjct: 356 AVKFRMEEPLSLSYSSSSSST-----TASSESSSESESLPRAEMLSRSSLDVKAPSRVSA 192
Query: 74 PK 75
K
Sbjct: 191 DK 186
>AW690594 similar to GP|23498163|emb hypothetical protein {Plasmodium
falciparum 3D7}, partial (10%)
Length = 633
Score = 30.8 bits (68), Expect = 0.25
Identities = 28/92 (30%), Positives = 40/92 (43%)
Frame = -3
Query: 8 RRLAPSLAARFRQNQKLTSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRMKELEA 67
R L S+++ + +S SSSSSSS ++ SSSSSS + +
Sbjct: 271 RVLILSISSSSPSSSSSSSSSSSSSSSSSSSSSCSSSSSSSSSSSSSSSCSSSSSSSCSS 92
Query: 68 SESATAPKTLRLSVETGGCSGFQYAFDLDDRI 99
S S+T+P S + CS F LD I
Sbjct: 91 SSSSTSP-----SSSSSLCSSTFRRFQLDSAI 11
>TC82350 similar to GP|9837385|gb|AAG00554.1| retinitis pigmentosa GTPase
regulator-like protein {Takifugu rubripes}, partial (2%)
Length = 759
Score = 30.0 bits (66), Expect = 0.43
Identities = 23/48 (47%), Positives = 27/48 (55%)
Frame = -2
Query: 26 SFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRMKELEASESATA 73
SFSSSSSSS DA++SSS PV T C + L S S+TA
Sbjct: 203 SFSSSSSSSSFSDASSSSS------PVSSTPYC---VSSLALSLSSTA 87
>TC84839 similar to GP|19386827|dbj|BAB86205. hypothetical protein~similar
to Arabidopsis thaliana chromosome 5 T6I14_10, partial
(20%)
Length = 819
Score = 30.0 bits (66), Expect = 0.43
Identities = 21/41 (51%), Positives = 25/41 (60%)
Frame = +2
Query: 21 NQKLTSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRR 61
N S S++SSSS L T SSSSSS V PV ++ LRR
Sbjct: 212 NNNNNSISNTSSSSSLRIFTISSSSSSQV-PVRISTVDLRR 331
>BF641220 weakly similar to PIR|G86203|G86 probable N-arginine dibasic
convertase [imported] - Arabidopsis thaliana, partial
(5%)
Length = 634
Score = 29.6 bits (65), Expect = 0.56
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = -2
Query: 25 TSFSSSSSSSVLHDATTSSSSSSHVEP 51
+S SSSSSSS +++SSSSSS +EP
Sbjct: 429 SSPSSSSSSSSSSSSSSSSSSSSSIEP 349
>TC78385 weakly similar to PIR|T21861|T21861 hypothetical protein F36F2.3 -
Caenorhabditis elegans, partial (1%)
Length = 1417
Score = 29.6 bits (65), Expect = 0.56
Identities = 23/64 (35%), Positives = 33/64 (50%), Gaps = 6/64 (9%)
Frame = +3
Query: 13 SLAARFRQNQKLTSFSSSSSSSVLHDATTSSSSS------SHVEPVHMTENCLRRMKELE 66
S A R R++ + +S S SSS L D++ S SSS SH H + ++ KE E
Sbjct: 183 SHAKRRRKHHRYSSDSDFYSSSSLSDSSRSESSSNSEHETSHRSKKHKKSDKPKKNKEKE 362
Query: 67 ASES 70
S+S
Sbjct: 363 RSKS 374
>TC78386 similar to SP|Q9NZW4|DSPP_HUMAN Dentin sialophosphoprotein
precursor [Contains: Dentin phosphoprotein (Dentin
phosphophoryn) (DPP);, partial (2%)
Length = 726
Score = 29.6 bits (65), Expect = 0.56
Identities = 23/64 (35%), Positives = 33/64 (50%), Gaps = 6/64 (9%)
Frame = +3
Query: 13 SLAARFRQNQKLTSFSSSSSSSVLHDATTSSSSS------SHVEPVHMTENCLRRMKELE 66
S A R R++ + +S S SSS L D++ S SSS SH H + ++ KE E
Sbjct: 192 SHAKRRRKHHRYSSDSDFYSSSSLSDSSRSESSSNSEHETSHRSKKHKKSDKPKKNKEKE 371
Query: 67 ASES 70
S+S
Sbjct: 372 RSKS 383
>TC86045 similar to PIR|T46225|T46225 alpha NAC-like protein - Arabidopsis
thaliana, partial (69%)
Length = 1166
Score = 29.3 bits (64), Expect = 0.73
Identities = 26/83 (31%), Positives = 37/83 (44%), Gaps = 7/83 (8%)
Frame = -1
Query: 7 FRRLAPSLAARFRQNQKLTSFSSSSSSSVLHDATTS---SSSSS----HVEPVHMTENCL 59
F AP+ A+ + +S SSSSSSS+ T+S +SSSS + V TE
Sbjct: 326 FEPSAPARASSLSSSSSSSSSSSSSSSSLSSSLTSSIIGASSSSGWGFFLRVVSSTEGIC 147
Query: 60 RRMKELEASESATAPKTLRLSVE 82
+ A S T P + +E
Sbjct: 146 CSTSDSVAGASITGPGAMVFEIE 78
>TC87242 homologue to PIR|S56716|S56716 protein kinase SPK-3 (EC 2.7.1.-) -
soybean, complete
Length = 1659
Score = 29.3 bits (64), Expect = 0.73
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = -2
Query: 24 LTSFSSSSSSSVLHDATTSSSSSSHVEPVHMT 55
L+ SSSSSSS +T+SSSSSS +P +T
Sbjct: 1487 LSYSSSSSSSSSTSSSTSSSSSSSPSQPKPLT 1392
Score = 28.9 bits (63), Expect = 0.95
Identities = 16/24 (66%), Positives = 19/24 (78%)
Frame = -2
Query: 24 LTSFSSSSSSSVLHDATTSSSSSS 47
L S+SSSSSSS ++TSSSSSS
Sbjct: 1490 LLSYSSSSSSSSSTSSSTSSSSSS 1419
>TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding protein -
Arabidopsis thaliana, partial (74%)
Length = 1909
Score = 28.9 bits (63), Expect = 0.95
Identities = 22/67 (32%), Positives = 36/67 (52%)
Frame = -2
Query: 14 LAARFRQNQKLTSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRMKELEASESATA 73
L+A ++ L S S+SSSSS ++TSSS+S+ + T + L L S S +
Sbjct: 399 LSASSSSSKGLLSSSTSSSSSSSSSSSTSSSTST---SSYSTSSSLDSS*SLSPSRSTCS 229
Query: 74 PKTLRLS 80
P+++ S
Sbjct: 228 PESVGFS 208
>TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Ateline
herpesvirus 3}, partial (8%)
Length = 986
Score = 28.5 bits (62), Expect = 1.2
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = -2
Query: 23 KLTSFSSSSSSSVLHDATTSSSSSS 47
K +S SSSSSSS+ +T SSSSSS
Sbjct: 751 KASSSSSSSSSSLSSSSTPSSSSSS 677
Score = 26.9 bits (58), Expect = 3.6
Identities = 18/44 (40%), Positives = 25/44 (55%)
Frame = -2
Query: 3 TSSLFRRLAPSLAARFRQNQKLTSFSSSSSSSVLHDATTSSSSS 46
+SSL PS ++ + +S SSSSSSS ++SSSSS
Sbjct: 724 SSSLSSSSTPSSSSSSPPSSSSSSSSSSSSSSSWSSPSSSSSSS 593
>TC84341 homologue to GP|48188|emb|CAA77863.1|| N-terminus of beta subunit
of RNA polymerase {Thermotoga maritima}, partial (3%)
Length = 687
Score = 28.5 bits (62), Expect = 1.2
Identities = 18/45 (40%), Positives = 22/45 (48%)
Frame = -2
Query: 11 APSLAARFRQNQKLTSFSSSSSSSVLHDATTSSSSSSHVEPVHMT 55
A S + F + SFSSSSS S L + S S SH E + T
Sbjct: 446 ATSPTSHFSSISSIFSFSSSSSPSFLSPSGAKSLSISHFEHMSST 312
>AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapiens},
partial (2%)
Length = 466
Score = 28.5 bits (62), Expect = 1.2
Identities = 18/48 (37%), Positives = 28/48 (57%)
Frame = +1
Query: 25 TSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRMKELEASESAT 72
+S SSSSSSS +++SSSSSS+ + E +K+ A ++T
Sbjct: 313 SSSSSSSSSSSSSSSSSSSSSSSNSDDEEEEEEAKPVVKKTPAKSAST 456
Score = 26.2 bits (56), Expect = 6.1
Identities = 18/50 (36%), Positives = 28/50 (56%)
Frame = +1
Query: 25 TSFSSSSSSSVLHDATTSSSSSSHVEPVHMTENCLRRMKELEASESATAP 74
+S SSSSSSS +++SSSSSS E + + + ++SA+ P
Sbjct: 310 SSSSSSSSSSSSSSSSSSSSSSSSNSDDEEEEEEAKPVVKKTPAKSASTP 459
>TC87729 similar to GP|9759461|dbj|BAB10377.1 gene_id:MAF19.15~unknown protein
{Arabidopsis thaliana}, partial (37%)
Length = 2210
Score = 28.1 bits (61), Expect = 1.6
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = -2
Query: 25 TSFSSSSSSSVLHDATTSSSSSSHV 49
+S+S+S SSS++ D SSSSSS V
Sbjct: 1702 SSYSASESSSLIRDRGLSSSSSSSV 1628
>TC83341 weakly similar to GP|8978076|dbj|BAA98104.1 gene_id:K14A3.4~unknown
protein {Arabidopsis thaliana}, partial (80%)
Length = 1253
Score = 28.1 bits (61), Expect = 1.6
Identities = 15/28 (53%), Positives = 21/28 (74%)
Frame = -1
Query: 24 LTSFSSSSSSSVLHDATTSSSSSSHVEP 51
++SFS+SSSSS +++SSSSSS P
Sbjct: 659 VSSFSTSSSSSSSSSSSSSSSSSSS*SP 576
>TC89370 weakly similar to PIR|T01856|T01856 hypothetical protein F9D12.19 -
Arabidopsis thaliana, partial (15%)
Length = 710
Score = 28.1 bits (61), Expect = 1.6
Identities = 16/36 (44%), Positives = 24/36 (66%), Gaps = 2/36 (5%)
Frame = +1
Query: 24 LTSFSSSSSSSVLHD--ATTSSSSSSHVEPVHMTEN 57
L+S SSSSSSS +H + ++SSS+ +P + T N
Sbjct: 154 LSSSSSSSSSSSIHHRLVSVAASSSTQTQPRNNTNN 261
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.126 0.343
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,824,096
Number of Sequences: 36976
Number of extensions: 77865
Number of successful extensions: 1534
Number of sequences better than 10.0: 86
Number of HSP's better than 10.0 without gapping: 870
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1197
length of query: 161
length of database: 9,014,727
effective HSP length: 88
effective length of query: 73
effective length of database: 5,760,839
effective search space: 420541247
effective search space used: 420541247
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0010.21