BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.15
(268 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 298 2e-81
TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 196 8e-51
AW559955 similar to GP|21593487|gb| unknown {Arabidopsis thalian... 41 4e-04
TC80629 35 0.030
TC77445 homologue to GP|19911575|dbj|BAB86891. syringolide-induc... 34 0.052
TC89113 homologue to GP|19911575|dbj|BAB86891. syringolide-induc... 32 0.20
AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapi... 30 0.98
BQ143784 weakly similar to SP|P33485|VNUA Probable nuclear antig... 30 1.3
BI309373 30 1.3
TC79792 similar to GP|14335158|gb|AAK59859.1 AT3g18420/MYF24_13 ... 29 1.7
TC78487 similar to PIR|A84663|A84663 probable beta-ketoacyl-CoA ... 29 2.2
BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, pa... 28 3.7
AL365936 28 4.8
TC81790 similar to GP|19347844|gb|AAL86002.1 unknown protein {Ar... 28 4.8
BF633248 similar to SP|P45598|ARAE_ Arabinose-proton symporter (... 28 4.8
TC84479 ENBP1 28 4.8
TC78855 similar to GP|15147869|dbj|BAB62845. Ndr kinase {Arabido... 28 4.8
TC80942 GP|21112489|gb|AAM40721.1 conserved hypothetical protein... 28 4.8
TC81337 similar to GP|15723293|gb|AAL06332.1 U2 auxiliary factor... 27 6.3
AJ501129 weakly similar to GP|10177856|dbj phloem-specific lecti... 27 6.3
>TC86353 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (12%)
Length = 1351
Score = 298 bits (762), Expect = 2e-81
Identities = 182/300 (60%), Positives = 210/300 (69%), Gaps = 32/300 (10%)
Frame = +1
Query: 1 MQQAETLVSACAGGSIDRKITCETLAN--KTDPP----ERHPDSPPESFWLSKDEEHDWF 54
M Q ETLVSACAGGS DRKI CET A+ KTDPP HPDSPPESFWLS+DEE++W
Sbjct: 67 MLQVETLVSACAGGSTDRKIACETQADDLKTDPPPESSHHHPDSPPESFWLSRDEENEWL 246
Query: 55 DRNALYERKESTKGSTSS------------STTNSQRFSLNLKSR--IIGLPKPQKPSFT 100
DRN +YERKESTKG++SS ST+NSQRF+ NLKS+ +IGLPKPQKPSF
Sbjct: 247 DRNFIYERKESTKGNSSSTNLNPNSNSNSNSTSNSQRFA-NLKSKTSMIGLPKPQKPSF- 420
Query: 101 DAKNRRNHKPCNNIKLFPKRSVSVTKSVTSHAEPSSPKVSCMGRVRSKRDRSRSRSLRLR 160
DAKN RNHKP +NIKLFPKRS SV K S EPSSPKVSCMG+VRSKR ++ + +
Sbjct: 421 DAKNLRNHKP-SNIKLFPKRSASVGK---SFVEPSSPKVSCMGKVRSKRGKTST----VT 576
Query: 161 NSRKSSIDAGEENPGRIGRKHGFFERFRAIFRSGRRKKAHRETDSAAEDSTV----TNSV 216
+ K+++ R KH FF+ FRAIFRSG R K R DS+A DS+ TNSV
Sbjct: 577 AAAKAAVKEKTARTERKQSKHSFFKSFRAIFRSGGRNKHDRMNDSSALDSSSITKNTNSV 756
Query: 217 --VKARDSTASVNDASFAESIS------SEPPGLGGMMRFASGRRSESWGVGESEIHVSR 268
KARDSTAS+ND SF ES++ EPPGLG MMRF SGRRSESW V +SEIHVSR
Sbjct: 757 SDSKARDSTASLNDVSFVESVTRNSVSEGEPPGLGVMMRFTSGRRSESW-VDDSEIHVSR 933
>TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (13%)
Length = 1108
Score = 196 bits (498), Expect = 8e-51
Identities = 127/270 (47%), Positives = 159/270 (58%), Gaps = 21/270 (7%)
Frame = +3
Query: 5 ETLVSACAGGSIDRKITCETLANKTDPPERHPDSPPESFWLSKDEEHDWFDRNALYERKE 64
ETLVSA +G + D K+ CE P + DSPPESFWLS D E+DW+DRNA+YER E
Sbjct: 324 ETLVSALSGVANDSKVACE-------PTDEAEDSPPESFWLSNDAEYDWWDRNAVYERNE 482
Query: 65 STKGSTSS----STTNSQRFSLNLK----SRIIGLPKPQKPSFTDAKN-RRNHKPCNNIK 115
STK S+ S S +NSQRFS NLK + IIGLPK QK SF +A+ RRNH N +
Sbjct: 483 STKASSISINPNSASNSQRFSKNLKKSKATTIIGLPKSQKASFAEARGCRRNHHKPGNTR 662
Query: 116 LFPKRSVSV-TKSVTSHAEPSSPKVSCMGRVRSKRDRSRSRSLRLRNSRKSSIDAGEENP 174
LFPKRS S+ KS ++ EPSSPKVSC+GRVRSK R +R LR R SS G
Sbjct: 663 LFPKRSASIGGKSDSTVFEPSSPKVSCIGRVRSK--RGHNRRLRTRQRSISSTATGVSAA 836
Query: 175 GRI----------GRKHGFFERFRAIFRSGRRKKAHRETDSAAEDSTVTNSVVKARDS-T 223
G + +K GF E +IFRS RR+K ++++ EDS+ R +
Sbjct: 837 GVVRQKSSRSTQRKKKTGFIESVCSIFRSHRREKTVQKSNPPVEDSSTKKKRNNGRKTRE 1016
Query: 224 ASVNDASFAESISSEPPGLGGMMRFASGRR 253
S + +++ SEP GLG M RFAS RR
Sbjct: 1017GSTVSRTVEDTVHSEPAGLGSMNRFASRRR 1106
>AW559955 similar to GP|21593487|gb| unknown {Arabidopsis thaliana}, partial
(7%)
Length = 411
Score = 41.2 bits (95), Expect = 4e-04
Identities = 35/147 (23%), Positives = 52/147 (34%), Gaps = 2/147 (1%)
Frame = +1
Query: 110 PCNNIKLFPKRSVSVTKSVTSHA--EPSSPKVSCMGRVRSKRDRSRSRSLRLRNSRKSSI 167
P ++ LFPK + K S + EP+SPKVSCMG ++SK+ + +
Sbjct: 49 PTTHVLLFPKEARRKHKRGISFSPKEPTSPKVSCMGEIKSKKKKKK-------------- 186
Query: 168 DAGEENPGRIGRKHGFFERFRAIFRSGRRKKAHRETDSAAEDSTVTNSVVKARDSTASVN 227
+ KK H+ + + V + K
Sbjct: 187 ---------------------------KEKKLHKGIQNVVPNKNVLLWISKGSYEGGRQG 285
Query: 228 DASFAESISSEPPGLGGMMRFASGRRS 254
+I S PP L M +F SGR S
Sbjct: 286 GKEKESAIVSVPPTLNSMKKFTSGRGS 366
>TC80629
Length = 847
Score = 35.0 bits (79), Expect = 0.030
Identities = 47/159 (29%), Positives = 65/159 (40%), Gaps = 15/159 (9%)
Frame = +1
Query: 91 LPKPQKPSFTDAKNR---RNHKPCNNIKLFPKRSVSVTKSVTSHAEPSSPKVSCMGRVRS 147
L K + S T A R R HK K +R VS++ S ++ +S RS
Sbjct: 187 LKKNKHSSKTKASGRSKSRKHKS----KKVRRREVSLSSSDYDDSKSMDTSLSSSSEDRS 354
Query: 148 KRDRSRSRSLRLRNSRKSSI-------DAGEEN-----PGRIGRKHGFFERFRAIFRSGR 195
KR R RSR + SRK + D+ E++ ++ RK E +S R
Sbjct: 355 KRKRERSRPRKDVKSRKKKVRRRSYSSDSSEDSLYAKKRKKVKRKDKHDETKE---KSRR 525
Query: 196 RKKAHRETDSAAEDSTVTNSVVKARDSTASVNDASFAES 234
+KK RET ++ S V DS ND S ES
Sbjct: 526 KKKVKRETSVSSVSSGSRRDVQLGIDS----NDNSEYES 630
>TC77445 homologue to GP|19911575|dbj|BAB86891. syringolide-induced protein
14-1-1 {Glycine max}, partial (22%)
Length = 978
Score = 34.3 bits (77), Expect = 0.052
Identities = 30/124 (24%), Positives = 53/124 (42%)
Frame = +2
Query: 133 EPSSPKVSCMGRVRSKRDRSRSRSLRLRNSRKSSIDAGEENPGRIGRKHGFFERFRAIFR 192
EP+SPK+SCMG+++ + ++ + + + K + RI F +R +A F
Sbjct: 329 EPTSPKISCMGQIKHHKKKNIPKPNKDLFTPKVDTEVKRSKFQRI-----FSQRSKAKF- 490
Query: 193 SGRRKKAHRETDSAAEDSTVTNSVVKARDSTASVNDASFAESISSEPPGLGGMMRFASGR 252
A R++D + F + +++ P +G M RFASGR
Sbjct: 491 ------AERKSDG-----------------------SGFVDEGNNKAPPMGDMRRFASGR 583
Query: 253 RSES 256
+ S
Sbjct: 584 EAFS 595
>TC89113 homologue to GP|19911575|dbj|BAB86891. syringolide-induced protein
14-1-1 {Glycine max}, partial (24%)
Length = 703
Score = 32.3 bits (72), Expect = 0.20
Identities = 13/48 (27%), Positives = 31/48 (64%), Gaps = 3/48 (6%)
Frame = +2
Query: 116 LFPKRSVSVTKSVTS--HAEPSSPKVSCMGRVRSKRDR-SRSRSLRLR 160
+ P+ + +K + + EP+SPK+SCMG+++ K+ + +S+ + ++
Sbjct: 320 IIPREARRKSKDINGIDYEEPTSPKISCMGQIKHKKKQIKKSKDMEVK 463
>AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapiens},
partial (2%)
Length = 466
Score = 30.0 bits (66), Expect = 0.98
Identities = 26/117 (22%), Positives = 52/117 (44%), Gaps = 2/117 (1%)
Frame = +1
Query: 124 VTKSVTSHAEPSSPKVSCMGRVRSKRDRSRSRSL--RLRNSRKSSIDAGEENPGRIGRKH 181
VTK+ +HA+ +S K+ S D S S S + + +K ++ A + P
Sbjct: 103 VTKTAPTHAKSASSKMEVDSDSDSDSDSSSSSSTSSSVSSVKKPAVSAAKATPA------ 264
Query: 182 GFFERFRAIFRSGRRKKAHRETDSAAEDSTVTNSVVKARDSTASVNDASFAESISSE 238
KK ++DS ++ S+ ++S + S++S + +S + S +S+
Sbjct: 265 ---------------KKEESDSDSDSDSSSSSSSSSSSSSSSSSSSSSSSSSSSNSD 390
>BQ143784 weakly similar to SP|P33485|VNUA Probable nuclear antigen. [strain
Kaplan PRV] {Pseudorabies virus}, partial (2%)
Length = 1022
Score = 29.6 bits (65), Expect = 1.3
Identities = 33/135 (24%), Positives = 46/135 (33%), Gaps = 15/135 (11%)
Frame = +1
Query: 92 PKPQKPSFTDAKNRRNHKPCNNIKLFPKRSVSVTKSVTSHAEPSSPKVSCMGRVRSKRDR 151
P P +P DA H P N I P+ + AE P+ GR +++ +
Sbjct: 256 PPPCQPPKKDAPGDTRHPPGNPITPHPR---------PTRAENGGPQEKATGRAKNRPRK 408
Query: 152 SRSRSLRLRNSRK-----------SSIDAG----EENPGRIGRKHGFFERFRAIFRSGRR 196
RS + ++ K S D G E P + H E A R R
Sbjct: 409 KRSPPTTIASAPKRTGPTRKRKGDSEDDPGPPRTETQPHHPPKSHDEGEVADATPRRTRE 588
Query: 197 KKAHRETDSAAEDST 211
H ET+ E T
Sbjct: 589 AHEHAETNHRPEPDT 633
>BI309373
Length = 599
Score = 29.6 bits (65), Expect = 1.3
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 180 KHGFFERFRAIFRSGRRKKAHRETDSAAE 208
KHG+F F +S RR + HR D+ A+
Sbjct: 81 KHGYFSSFNMSVKSLRRHRVHRLVDTGAQ 167
>TC79792 similar to GP|14335158|gb|AAK59859.1 AT3g18420/MYF24_13
{Arabidopsis thaliana}, partial (64%)
Length = 1289
Score = 29.3 bits (64), Expect = 1.7
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +2
Query: 92 PKPQKPSFTDAKNRRNHKPCNNIKLFPKRSVSVTKSVTSH 131
P PQ P + NH C +I FP +S + T + SH
Sbjct: 248 PPPQNPRRISTLHCCNHHRCTHIPRFPSQSRTTTDTHRSH 367
>TC78487 similar to PIR|A84663|A84663 probable beta-ketoacyl-CoA synthase
[imported] - Arabidopsis thaliana, partial (97%)
Length = 1999
Score = 28.9 bits (63), Expect = 2.2
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = -3
Query: 215 SVVKARDSTASVNDASFAESISSEPPGLGGMMRFASGR 252
+VV S+ + N + A ++S GLGGM+R ASGR
Sbjct: 809 TVVFENSSSIAPNITASASFLASFIEGLGGMLRTASGR 696
>BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, partial (2%)
Length = 1320
Score = 28.1 bits (61), Expect = 3.7
Identities = 16/55 (29%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Frame = +1
Query: 149 RDRSRSRSLRLRNSRKSSIDAGEENPGRIG--RKHGFFERFRAIFRSGRRKKAHR 201
R+R+R + + ++ S +GE+ P R+G RK + R ++ RRK+ R
Sbjct: 1144 RERARRKGRPVTDAESGSARSGEDEPARVGRTRKTKTRDNSRRGTKNERRKEGER 1308
>AL365936
Length = 441
Score = 27.7 bits (60), Expect = 4.8
Identities = 19/58 (32%), Positives = 24/58 (40%)
Frame = +2
Query: 62 RKESTKGSTSSSTTNSQRFSLNLKSRIIGLPKPQKPSFTDAKNRRNHKPCNNIKLFPK 119
R S K ST + N N K I+G+P+P K K + P N FPK
Sbjct: 167 RNPSLKQSTHQTPQNQTMKFPNPKL-IMGMPRPPKSRNPSLKQSTHQTPQNQTMKFPK 337
>TC81790 similar to GP|19347844|gb|AAL86002.1 unknown protein {Arabidopsis
thaliana}, partial (5%)
Length = 505
Score = 27.7 bits (60), Expect = 4.8
Identities = 15/34 (44%), Positives = 18/34 (52%), Gaps = 4/34 (11%)
Frame = -3
Query: 238 EPPGLGGMMRFASGRRSE----SWGVGESEIHVS 267
EPPG G+ ASGR S SW + ES + S
Sbjct: 260 EPPGPSGLSSGASGRSSSSEATSWMISESSMKTS 159
>BF633248 similar to SP|P45598|ARAE_ Arabinose-proton symporter (Arabinose
transporter). {Klebsiella oxytoca}, partial (5%)
Length = 514
Score = 27.7 bits (60), Expect = 4.8
Identities = 9/29 (31%), Positives = 16/29 (55%)
Frame = -2
Query: 42 SFWLSKDEEHDWFDRNALYERKESTKGST 70
++W+ ++ H W DR + +E T ST
Sbjct: 123 TWWIQRNRSHGWMDR*VIITHRERTVDST 37
>TC84479 ENBP1
Length = 5108
Score = 27.7 bits (60), Expect = 4.8
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +1
Query: 202 ETDSAAEDSTVTNSVVKARDSTASVNDASFAESISSEPPGLGGMMR 247
E D E +T + + R S SVN+ E +SSE G G + R
Sbjct: 1180 EIDGVGE---ITGPMERGRKSKGSVNEEKNVEEVSSEVAGAGEIAR 1308
>TC78855 similar to GP|15147869|dbj|BAB62845. Ndr kinase {Arabidopsis
thaliana}, partial (89%)
Length = 1642
Score = 27.7 bits (60), Expect = 4.8
Identities = 14/36 (38%), Positives = 20/36 (54%)
Frame = -1
Query: 222 STASVNDASFAESISSEPPGLGGMMRFASGRRSESW 257
+T S +S ++ PPG MMR+ R+SESW
Sbjct: 742 ATCSERISSLINNVIMSPPGKYSMMRY---RKSESW 644
>TC80942 GP|21112489|gb|AAM40721.1 conserved hypothetical protein
{Xanthomonas campestris pv. campestris str. ATCC 33913},
partial (9%)
Length = 482
Score = 27.7 bits (60), Expect = 4.8
Identities = 24/96 (25%), Positives = 39/96 (40%), Gaps = 12/96 (12%)
Frame = -2
Query: 85 KSRIIGLPKPQKPSFTDAKNRRNH---------KPCNNIKLFP---KRSVSVTKSVTSHA 132
KS I P P KP++ K + N+ KP + + P K V KS T +
Sbjct: 397 KSEAIYTPNPTKPNYEVPKTKTNYEYAPAPKIEKPKSEVVHTPNPTKPDYEVPKSKTDYG 218
Query: 133 EPSSPKVSCMGRVRSKRDRSRSRSLRLRNSRKSSID 168
+PK+ ++S+S ++ N KS +
Sbjct: 217 YAPTPKI----------EKSKSEAVYTPNPTKSDYE 140
>TC81337 similar to GP|15723293|gb|AAL06332.1 U2 auxiliary factor small
subunit {Arabidopsis thaliana}, partial (81%)
Length = 1190
Score = 27.3 bits (59), Expect = 6.3
Identities = 23/64 (35%), Positives = 29/64 (44%), Gaps = 4/64 (6%)
Frame = +2
Query: 145 VRSKRDRSRSRSLRLRNSRKSSIDAGEENPGR----IGRKHGFFERFRAIFRSGRRKKAH 200
V S+R RSRSRS R R+S E R GR+ +R R R RK+
Sbjct: 635 VSSQRSRSRSRSNSPRRRRRSRSRERERPRDRDYDSRGRRSSDRDRVRDGDRDRERKRDS 814
Query: 201 RETD 204
R+ D
Sbjct: 815 RDRD 826
>AJ501129 weakly similar to GP|10177856|dbj phloem-specific lectin-like
protein {Arabidopsis thaliana}, partial (33%)
Length = 558
Score = 27.3 bits (59), Expect = 6.3
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = -1
Query: 192 RSGRRKKAHRETDSAAEDSTVTNSVVKARDSTASVNDASFAESISSEPPG 241
+S RK++ +DSAAE T K R+S ++ S PPG
Sbjct: 156 KSDGRKRSQTISDSAAEQKLTTERQRKDRNSRERTEKSASLAVDGSAPPG 7
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.126 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,799,831
Number of Sequences: 36976
Number of extensions: 106069
Number of successful extensions: 665
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 636
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 648
length of query: 268
length of database: 9,014,727
effective HSP length: 94
effective length of query: 174
effective length of database: 5,538,983
effective search space: 963783042
effective search space used: 963783042
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0010.15