BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0005.12
(526 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding... 44 1e-04
TC79604 similar to GP|18175762|gb|AAL59923.1 unknown protein {Ar... 38 0.011
TC89204 similar to GP|16226341|gb|AAL16140.1 AT3g11400/F24K9_7 {... 35 0.054
TC77695 similar to PIR|T09704|T09704 probable arginine/serine-ri... 35 0.054
BQ143784 weakly similar to SP|P33485|VNUA Probable nuclear antig... 35 0.054
AJ503118 similar to GP|18175762|gb| unknown protein {Arabidopsis... 35 0.092
TC91646 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproli... 35 0.092
TC84935 similar to PIR|G96631|G96631 probable RNA-binding protei... 34 0.12
BQ151301 similar to PIR|S34666|S346 glycine-rich protein - commo... 34 0.16
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 33 0.21
TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garde... 33 0.21
BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich gl... 33 0.27
TC84335 weakly similar to GP|21741678|emb|CAD40580. oj000126_13.... 33 0.35
BQ751138 weakly similar to GP|15991793|gb platelet binding prote... 32 0.46
BQ137259 homologue to GP|15387793|emb hypothetical predicted pro... 32 0.46
TC77694 homologue to PIR|T09704|T09704 probable arginine/serine-... 32 0.46
BE942328 weakly similar to GP|21741678|emb oj000126_13.2 {Oryza ... 32 0.46
AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding pr... 32 0.60
TC76556 similar to PIR|S71779|S71779 glycine-rich RNA-binding pr... 32 0.60
BG449291 similar to GP|2204089|emb| FCA gamma {Arabidopsis thali... 30 3.0
>TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding protein -
Arabidopsis thaliana, partial (74%)
Length = 1909
Score = 44.3 bits (103), Expect = 1e-04
Identities = 26/91 (28%), Positives = 45/91 (48%)
Frame = +2
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRK 126
++V + +++ +++LF HGK+T V L + K R+GFV +A+R MK
Sbjct: 959 VYVKNLPENITQNRLKELFEHHGKITKVALPPAKAGQEKSRYGFVHFADRSSAMKALKNT 1138
Query: 127 QSYVSMAPPTTVKDSRAKERLHLSGGFASSS 157
+ Y T++ S AK + AS+S
Sbjct: 1139 EKYEING--QTLECSLAKPQADQKSSGASNS 1225
>TC79604 similar to GP|18175762|gb|AAL59923.1 unknown protein {Arabidopsis
thaliana}, partial (56%)
Length = 727
Score = 37.7 bits (86), Expect = 0.011
Identities = 23/69 (33%), Positives = 35/69 (50%)
Frame = +2
Query: 54 ARVVAPPARTCFPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRY 113
AR +A P+ LFV G++ +R+ F + G++ + + R G FGFV+Y
Sbjct: 215 ARQMAEPSTN---LFVSGLSKRTTTETLREAFQKFGEVVHARVVTDRVSGYSKGFGFVKY 385
Query: 114 ANREDVMKG 122
A ED KG
Sbjct: 386 ATLEDAAKG 412
>TC89204 similar to GP|16226341|gb|AAL16140.1 AT3g11400/F24K9_7 {Arabidopsis
thaliana}, partial (80%)
Length = 1087
Score = 35.4 bits (80), Expect = 0.054
Identities = 16/61 (26%), Positives = 30/61 (48%)
Frame = +1
Query: 69 VDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRKQS 128
V +++ + +LF G ++ V++ +N G FGFV + NRED + ++
Sbjct: 625 VTNLSEDTREPDLHELFGTFGSVSRVYVAIDKNTGTSRGFGFVNFVNREDAQRAINKLNG 804
Query: 129 Y 129
Y
Sbjct: 805 Y 807
>TC77695 similar to PIR|T09704|T09704 probable arginine/serine-rich splicing
factor - alfalfa, partial (83%)
Length = 1657
Score = 35.4 bits (80), Expect = 0.054
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 3/79 (3%)
Frame = +1
Query: 84 LFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRKQSYVSMAPPTTVKDSR- 142
LF ++GK+ ++F+ R R G F FVRY ++ K R + TV+ ++
Sbjct: 169 LFDKYGKVVDIFIPRDRRTGESRGFAFVRYKYADEASKAVDRLDGRMVDGREITVQFAKY 348
Query: 143 --AKERLHLSGGFASSSRS 159
ER+H +S RS
Sbjct: 349 GPNAERIHKGRIIETSPRS 405
>BQ143784 weakly similar to SP|P33485|VNUA Probable nuclear antigen. [strain
Kaplan PRV] {Pseudorabies virus}, partial (2%)
Length = 1022
Score = 35.4 bits (80), Expect = 0.054
Identities = 20/56 (35%), Positives = 25/56 (43%), Gaps = 5/56 (8%)
Frame = +3
Query: 439 PPRPRPRG-----RPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPP 489
PP PRPRG +P +K+ RR +GHP G P H +PPP
Sbjct: 198 PPPPRPRGGGLPRQPTKKQTPSMPAPKKRRTRGHPPPPG--------KPHHAAPPP 341
>AJ503118 similar to GP|18175762|gb| unknown protein {Arabidopsis thaliana},
partial (40%)
Length = 377
Score = 34.7 bits (78), Expect = 0.092
Identities = 19/56 (33%), Positives = 28/56 (49%)
Frame = +1
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKG 122
+FV G+ +R+ F + G++ V + R G FG+VRYA ED KG
Sbjct: 205 VFVTGLNKRTTSETLREAFKKFGEVKQVRVVADRVSGYSKGFGYVRYATSEDAAKG 372
>TC91646 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproline-rich
glycoprotein DZ-HRGP {Volvox carteri f. nagariensis},
partial (15%)
Length = 984
Score = 34.7 bits (78), Expect = 0.092
Identities = 23/61 (37%), Positives = 27/61 (43%), Gaps = 5/61 (8%)
Frame = +1
Query: 438 SPPRPRPRGRPKRKKEVKRGLEGS-----RRPQGHPRKSGTMDLDAAPSPSHPSPPPQLH 492
SPPRP PR P++ R L S R P R + T +PSPS SPP
Sbjct: 370 SPPRPPPRSPPRQLMSPPRPLPRSPSPPPRNPPELSRTTPTPRPPMSPSPSPTSPPAATP 549
Query: 493 P 493
P
Sbjct: 550 P 552
>TC84935 similar to PIR|G96631|G96631 probable RNA-binding protein F8A5.17
[imported] - Arabidopsis thaliana, partial (41%)
Length = 552
Score = 34.3 bits (77), Expect = 0.12
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 8/100 (8%)
Frame = +2
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANR---EDVMK-- 121
+FV G+ V + F ++GK+ + +R+ GR FGF+ +++R ED +K
Sbjct: 143 IFVGGLGWDVTERQLEHAFDRYGKILECQIMMERDTGRPRGFGFITFSDRRGMEDAIKEM 322
Query: 122 -GRSRKQSYVSM--APPTTVKDSRAKERLHLSGGFASSSR 158
GR +S+ A P D A +R GGF+S R
Sbjct: 323 DGREIGDRIISVNKAQPRMGGDD-ADQRYR--GGFSSGGR 433
>BQ151301 similar to PIR|S34666|S346 glycine-rich protein - common tobacco,
partial (22%)
Length = 530
Score = 33.9 bits (76), Expect = 0.16
Identities = 28/103 (27%), Positives = 37/103 (35%), Gaps = 15/103 (14%)
Frame = +2
Query: 437 FSPPRP----------RPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPS--- 483
FSPP P RP+ P +K+ K+ + P P ++G + + P P
Sbjct: 20 FSPPPPCPKVGGGGRKRPKPTPPPQKQKKK--KPHHPPPPPPHEAGAVIMPPPPPPPPPP 193
Query: 484 --HPSPPPQLHPAHITRGLQRLGFWQNPQACLFREVMKRPFEG 524
HP PPP P H P LF PF G
Sbjct: 194 TWHPPPPPPTPPTH-------------PPPTLFFLFFSHPFPG 283
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 33.5 bits (75), Expect = 0.21
Identities = 18/60 (30%), Positives = 26/60 (43%), Gaps = 3/60 (5%)
Frame = +2
Query: 439 PPRPRPRGRPKRKKEVKRGLEGSRRPQGH---PRKSGTMDLDAAPSPSHPSPPPQLHPAH 495
PP P+P P +K+ K ++PQ + P K +A+ P P PP P H
Sbjct: 11 PPNPQPPSDPPKKRTPKPETPNRKKPQKNKKEPPKKRKTPPEASHPPPRPRPPRPPQPNH 190
Score = 32.3 bits (72), Expect = 0.46
Identities = 17/54 (31%), Positives = 27/54 (49%)
Frame = +3
Query: 440 PRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQLHP 493
PR +PRG +R+ + K + +PQ P K+ P P+ +PPP +P
Sbjct: 768 PRKKPRGAREREGKKKNPQKKPPKPQQQPPKN--------PPPTKKTPPPPKNP 905
Score = 30.4 bits (67), Expect = 1.7
Identities = 20/65 (30%), Positives = 25/65 (37%)
Frame = +2
Query: 433 PVLDFSPPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQLH 492
P PPRPRP P+ + + + + HP T P HP PP H
Sbjct: 131 PEASHPPPRPRPPRPPQPNHQHDKPQKTT-----HPHHPHT-----PPPTPHPPNPPPNH 280
Query: 493 PAHIT 497
P H T
Sbjct: 281 PNHPT 295
Score = 28.1 bits (61), Expect = 8.6
Identities = 16/59 (27%), Positives = 22/59 (37%)
Frame = +2
Query: 431 DDPVLDFSPPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPP 489
+ P P + P PKR+ + + G P HP + G P P PPP
Sbjct: 506 EGPATHGPPEKKTPPPTPKRRGKHTKRAPGGGPPPPHPGRGG-------GGPGGPPPPP 661
>TC76790 similar to PIR|T06817|T06817 RNA-binding protein - garden pea,
partial (79%)
Length = 1141
Score = 33.5 bits (75), Expect = 0.21
Identities = 22/93 (23%), Positives = 41/93 (43%)
Frame = +1
Query: 27 GGTFGDLSSVDDEGDDEGEQDGGDARVARVVAPPARTCFPLFVDGIADSVNYFHVRDLFL 86
G + S V ++ +G + R R P R ++V +A V+ + +F
Sbjct: 550 GYDYNGRSLVVNKASPKGSRPERTERAPRTFEPVLR----IYVANLAWEVDNSRLEQVFS 717
Query: 87 QHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV 119
+HGK+ + + R GR FGFV ++ ++
Sbjct: 718 EHGKIVSARVVYDRETGRSRGFGFVTMSDETEM 816
>BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (39%)
Length = 1358
Score = 33.1 bits (74), Expect = 0.27
Identities = 24/67 (35%), Positives = 30/67 (43%), Gaps = 11/67 (16%)
Frame = -3
Query: 438 SPPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGT----MDLDAAPSPSHPS------- 486
+P RPRPR P R +R G+R K GT A PSP+HP+
Sbjct: 444 APQRPRPRRPPARPPSGRR--RGARAKVRKLPKGGTPGSRAPSPAPPSPTHPAPRRPWTG 271
Query: 487 PPPQLHP 493
PPP+ P
Sbjct: 270 PPPRPRP 250
Score = 32.7 bits (73), Expect = 0.35
Identities = 21/71 (29%), Positives = 25/71 (34%)
Frame = -3
Query: 439 PPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQLHPAHITR 498
PPR R P+R SR P+ HP P P PPP P TR
Sbjct: 783 PPRTAQRHPPRRTLRASAAQHPSRAPRRHP-------------PPPPPPPPSSPPGMGTR 643
Query: 499 GLQRLGFWQNP 509
+ +W P
Sbjct: 642 PSCQENWWAAP 610
Score = 28.5 bits (62), Expect = 6.6
Identities = 22/66 (33%), Positives = 29/66 (43%), Gaps = 9/66 (13%)
Frame = -3
Query: 438 SPPRPRPRGRPKRKKEVK----RGLEGSRRPQGHPRKSGTMDLDAAPSPS-----HPSPP 488
S P P+ P R+ V+ R + R Q HP + T+ AA PS HP PP
Sbjct: 861 SSTSPAPQIPPLRRSIVRARAARAVRPPRTAQRHPPRR-TLRASAAQHPSRAPRRHPPPP 685
Query: 489 PQLHPA 494
P P+
Sbjct: 684 PPPPPS 667
>TC84335 weakly similar to GP|21741678|emb|CAD40580. oj000126_13.2 {Oryza
sativa}, partial (39%)
Length = 678
Score = 32.7 bits (73), Expect = 0.35
Identities = 21/82 (25%), Positives = 36/82 (43%), Gaps = 8/82 (9%)
Frame = +2
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGR--- 123
+FV G+A S + + F Q+G + + + + R FG+V +A E+ K +
Sbjct: 206 IFVKGLAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGM 385
Query: 124 -----SRKQSYVSMAPPTTVKD 140
+ YV M PP K+
Sbjct: 386 NGKILHGRVLYVDMDPPNEQKN 451
>BQ751138 weakly similar to GP|15991793|gb platelet binding protein GspB
{Streptococcus gordonii}, partial (1%)
Length = 703
Score = 32.3 bits (72), Expect = 0.46
Identities = 31/136 (22%), Positives = 48/136 (34%)
Frame = +2
Query: 351 SVLAAPRQVFGIGSLAAREVIGDSAYGVGVVSCDGLALGRPSHSCFEIGACSSFGSTSAE 410
S + + +Q G+G R G S G+G+V G C S ++++
Sbjct: 113 SSVRSAKQSLGLGKKGRR---GASGSGIGLV-----------------GGCVSASTSASA 232
Query: 411 ACNDSAREEDSLGVDSDAFADDPVLDFSPPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRK 470
+ + SA S P S P RP+ L SR GH +
Sbjct: 233 SASASASASASRSGSGSGSTSGPRRGKSSPALASHSRPQ-------SLSQSRSHSGHSHR 391
Query: 471 SGTMDLDAAPSPSHPS 486
S + +P P HP+
Sbjct: 392 SSSRISKTSPPPPHPA 439
>BQ137259 homologue to GP|15387793|emb hypothetical predicted protein
LM15-1.83 unknown function {Leishmania major}, partial
(3%)
Length = 1050
Score = 32.3 bits (72), Expect = 0.46
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 6/61 (9%)
Frame = +2
Query: 444 PRGRPKRKKEVKRGLEGSRRPQGHPRKSG------TMDLDAAPSPSHPSPPPQLHPAHIT 497
PRGR + K ++K R P+K G + AP P+ P+P + P T
Sbjct: 137 PRGRREEKNQIKADTTRPRTKYNQPKKKGAAKRRRARQIRRAPPPARPAPNTKNRPDSQT 316
Query: 498 R 498
R
Sbjct: 317 R 319
>TC77694 homologue to PIR|T09704|T09704 probable arginine/serine-rich
splicing factor - alfalfa, partial (95%)
Length = 1494
Score = 32.3 bits (72), Expect = 0.46
Identities = 14/42 (33%), Positives = 23/42 (54%)
Frame = +3
Query: 84 LFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSR 125
LF ++GK+ ++F+ + R G F FVRY ++ K R
Sbjct: 141 LFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYADEASKAVDR 266
>BE942328 weakly similar to GP|21741678|emb oj000126_13.2 {Oryza sativa},
partial (34%)
Length = 548
Score = 32.3 bits (72), Expect = 0.46
Identities = 20/80 (25%), Positives = 35/80 (43%)
Frame = +3
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRK 126
+FV G+A S + + F Q+G + + + + R FG+V +A E+ K +
Sbjct: 120 IFVKGLAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGM 299
Query: 127 QSYVSMAPPTTVKDSRAKER 146
V P K S ++ R
Sbjct: 300 NGKVRTYKPKVSKHSLSELR 359
>AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding protein
{Medicago sativa}, complete
Length = 437
Score = 32.0 bits (71), Expect = 0.60
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +1
Query: 68 FVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV 119
FV G+A + + + F Q+G++ + + R GR FGFV +AN + +
Sbjct: 67 FVGGLAWATDNEALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSM 222
>TC76556 similar to PIR|S71779|S71779 glycine-rich RNA-binding protein GRP1
- wheat, partial (95%)
Length = 754
Score = 32.0 bits (71), Expect = 0.60
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +2
Query: 68 FVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV 119
FV G+A + + + F Q+G++ + + R GR FGFV +AN + +
Sbjct: 110 FVGGLAWATDNEALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSM 265
>BG449291 similar to GP|2204089|emb| FCA gamma {Arabidopsis thaliana},
partial (9%)
Length = 593
Score = 29.6 bits (65), Expect = 3.0
Identities = 15/48 (31%), Positives = 24/48 (49%)
Frame = +2
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYA 114
LFV + + +R LF +HG + V L + R G+ F++YA
Sbjct: 389 LFVGSVPRTATEEDIRPLFEEHGNVVEVALIKDRKTGQHQGCCFIKYA 532
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.138 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,600,447
Number of Sequences: 36976
Number of extensions: 271495
Number of successful extensions: 2742
Number of sequences better than 10.0: 100
Number of HSP's better than 10.0 without gapping: 2338
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2661
length of query: 526
length of database: 9,014,727
effective HSP length: 101
effective length of query: 425
effective length of database: 5,280,151
effective search space: 2244064175
effective search space used: 2244064175
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0005.12