TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0004a.4
         (791 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BI265380 weakly similar to GP|17932898|em isoamylase {Triticum a...   162  8e-48
AW697292 homologue to GP|22136708|gb putative isoamylase {Arabid...   150  2e-36
CB065311 similar to PIR|C83375|C83 probable glycosyl hydrolase P...   141  1e-33
AW689078 similar to GP|22136708|gb putative isoamylase {Arabidop...    49  6e-06
BG587290                                                               33  0.42
TC91964 similar to GP|23496165|gb|AAN35825.1 hypothetical protei...    32  0.95
TC78360                                                                30  2.8
BE320423                                                               30  4.7
TC85575 similar to PIR|D86410|D86410 protein F3M18.16 [imported]...    29  6.1
TC87436 similar to PIR|T51575|T51575 2-hydroxyphytanoyl-CoA lyas...    29  8.0
TC91709 homologue to GP|21213868|emb|CAD12767. LHY protein {Phas...    29  8.0
TC77271 similar to PIR|T07929|T07929 probable long-chain-fatty-a...    29  8.0
CA860191 homologue to GP|19697342|gb hypothetical protein {Dicty...    29  8.0
BI262804 weakly similar to GP|11072027|gb F12A21.14 {Arabidopsis...    29  8.0

>BI265380 weakly similar to GP|17932898|em isoamylase {Triticum aestivum},
           partial (13%)
          Length = 604

 Score =  162 bits (409), Expect(2) = 8e-48
 Identities = 83/140 (59%), Positives = 106/140 (75%), Gaps = 1/140 (0%)
 Frame = +3

Query: 22  HSDSQCRVSLSKRVSEKHKSICSTTKILATGNGSGFETETTLV-VDKPQLGGRFQVSRGY 80
           H+ +   +S + R  +K K      KI A GN  G +TET ++ ++K QL    Q+S+G+
Sbjct: 138 HNRTSIIISPNSRPKKKKK------KIFAIGNRVGVDTETAVIEIEKRQL----QLSKGH 287

Query: 81  PAPFGATVRDGGVNFAIYSLNAVSATLCLFTLSDFQDNQVTEYITLDPLMNKTGSVWHVF 140
           P+P+GAT ++ GVNFAI SLN++SATLC FTLSDF++N+VTEYITLDPL+NKTG VWHVF
Sbjct: 288 PSPYGATPQEDGVNFAINSLNSLSATLCFFTLSDFKNNKVTEYITLDPLVNKTGCVWHVF 467

Query: 141 LKGDFGDMLYGYKFDGKFSP 160
           LKGDF DMLY YKFDGKFSP
Sbjct: 468 LKGDFKDMLYAYKFDGKFSP 527



 Score = 47.8 bits (112), Expect(2) = 8e-48
 Identities = 21/25 (84%), Positives = 23/25 (92%)
 Frame = +2

Query: 162 EGHYYDSSLILLDPYAKAVISRGEF 186
           +GHYYDSS IL+DPYAKAVISR EF
Sbjct: 530 QGHYYDSSRILIDPYAKAVISRXEF 604


>AW697292 homologue to GP|22136708|gb putative isoamylase {Arabidopsis
           thaliana}, partial (13%)
          Length = 343

 Score =  150 bits (379), Expect = 2e-36
 Identities = 70/127 (55%), Positives = 85/127 (66%)
 Frame = +2

Query: 374 KGEFYNYSGCGNTLNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWNGVN 433
           KG+  N+SGCGNTLNCNHPVV   I+D LR+WVTE HVDGFRFDLASI+ R +       
Sbjct: 5   KGQLLNFSGCGNTLNCNHPVVMDLILDSLRHWVTEYHVDGFRFDLASILCRGTD------ 166

Query: 434 VFGTSIEGDLLATGTPLVSPPLIDLISNDPILHGVKLIAEAWDAGGLYQVGTFPHWGIWS 493
                        G+PL +PPLI  I+ D +L   K+IAE WD GGLY VG+FP+W  W+
Sbjct: 167 -------------GSPLNAPPLIRAIAKDAVLSRCKIIAEPWDCGGLYLVGSFPNWDRWA 307

Query: 494 EWNGKYR 500
           EWNGKY+
Sbjct: 308 EWNGKYQ 328


>CB065311 similar to PIR|C83375|C83 probable glycosyl hydrolase PA2160
           [imported] - Pseudomonas aeruginosa (strain PAO1),
           partial (26%)
          Length = 567

 Score =  141 bits (355), Expect = 1e-33
 Identities = 62/128 (48%), Positives = 85/128 (65%)
 Frame = -2

Query: 537 NSINFVCTHDGFTLADLVTYNNKHNLPNGEDNNDGENHNNSWNCGQEGEFASSSVKKLRK 596
           +S+NF+  HDGFTL DLV+YN+KHN  N E+N DG ++N SWNCG EG      +  LR 
Sbjct: 566 SSVNFITAHDGFTLRDLVSYNHKHNEDNDENNQDGTDNNLSWNCGAEGPTDDPDINALRM 387

Query: 597 RQMRNFFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYLNYFQWDIKEESSSDFFRF 656
           RQMRNFF +L+ +QG PMI  GDE+  T+ GNNN YC D+ + +  W++ +E  +D   F
Sbjct: 386 RQMRNFFATLLFAQGTPMIVAGDEFSRTQHGNNNAYCQDSEIGWINWEL-DEDGADLLAF 210

Query: 657 CCLMTKFR 664
              +T+ R
Sbjct: 209 VKRLTRLR 186


>AW689078 similar to GP|22136708|gb putative isoamylase {Arabidopsis
           thaliana}, partial (13%)
          Length = 511

 Score = 49.3 bits (116), Expect = 6e-06
 Identities = 35/122 (28%), Positives = 57/122 (46%), Gaps = 2/122 (1%)
 Frame = +3

Query: 664 RYECESLGLDDFPTSDRLQWHGHFPGKPDWSE-TSRFVAFTMVDSVKREIYIAFNTSHLP 722
           R+  ++L  ++F + + + WH     + +W    S+F+AFT+ D    +IY+AFN     
Sbjct: 42  RHAHKTLSHENFLSENEITWH-----EDNWDNYESKFLAFTLHDKSGGDIYLAFNAHDYF 206

Query: 723 VTITLPERPGYR-WEPLVDTGKSAPYDFLTPDLPGRDIAIQQYAQFLDANLYPMLSYSSI 781
           +   LP  P  R W  +VDT   +P D +   +PG                Y +  YSSI
Sbjct: 207 LKALLPTPPTKRKWFRVVDTNLQSPDDIVLDGVPG------------IGETYSIAPYSSI 350

Query: 782 IL 783
           +L
Sbjct: 351 LL 356


>BG587290 
          Length = 667

 Score = 33.1 bits (74), Expect = 0.42
 Identities = 20/60 (33%), Positives = 33/60 (54%), Gaps = 1/60 (1%)
 Frame = +3

Query: 495 WNGKYRDTVRQFVKGTDGFAGAFAECLCGSPNVYQGGGRKPWNS-INFVCTHDGFTLADL 553
           W+ +YRD++  F+ G   F GA+   +  + +  Q GGR PW++ I  +  H  + L DL
Sbjct: 378 WSQQYRDSIVNFIFGLR-FKGAYFPWVLLAFDSLQMGGRIPWDTLIGIITGHIFYFLYDL 554


>TC91964 similar to GP|23496165|gb|AAN35825.1 hypothetical protein
           {Plasmodium falciparum 3D7}, partial (1%)
          Length = 827

 Score = 32.0 bits (71), Expect = 0.95
 Identities = 19/49 (38%), Positives = 25/49 (50%)
 Frame = +1

Query: 540 NFVCTHDGFTLADLVTYNNKHNLPNGEDNNDGENHNNSWNCGQEGEFAS 588
           N + T D  +  D    NN +N  N + NND +N NN+ N G   E AS
Sbjct: 370 NVIGTVDIVSTTDFSENNNDNN-NNNDKNNDNDNENNNDNGGGGNESAS 513


>TC78360 
          Length = 805

 Score = 30.4 bits (67), Expect = 2.8
 Identities = 16/59 (27%), Positives = 29/59 (49%)
 Frame = +1

Query: 523 GSPNVYQGGGRKPWNSINFVCTHDGFTLADLVTYNNKHNLPNGEDNNDGENHNNSWNCG 581
           GSP++ QG  +  W        HD +T  D+   + +  + +G  ++ G N+NN  + G
Sbjct: 142 GSPHLIQGQDQHVWEE---KVKHDPYTSHDMGVASRETKVKSGNGDHWGSNYNNGVSNG 309


>BE320423 
          Length = 210

 Score = 29.6 bits (65), Expect = 4.7
 Identities = 23/72 (31%), Positives = 27/72 (36%), Gaps = 11/72 (15%)
 Frame = +2

Query: 513 FAGAFAECLCGSPNVYQGGGRKPWNSINFVCTHDGFTLADLVTYNNKHN----------- 561
           F G       G PN YQ G RKP    N   THDG+       ++N +            
Sbjct: 2   FGGDHRATSVGDPNPYQEG-RKPIGGGNLRSTHDGY-------HDNGYGGDKEGRKPVGI 157

Query: 562 LPNGEDNNDGEN 573
            P G  N DG N
Sbjct: 158 SPTGNSNYDGPN 193


>TC85575 similar to PIR|D86410|D86410 protein F3M18.16 [imported] -
           Arabidopsis thaliana, partial (15%)
          Length = 1225

 Score = 29.3 bits (64), Expect = 6.1
 Identities = 23/121 (19%), Positives = 43/121 (35%), Gaps = 5/121 (4%)
 Frame = +3

Query: 524 SPNVYQGGGRKPWNSINFVCTHDGFTLADLVT--YNNKHNLPNGEDNNDGENHNNSWNCG 581
           +P  Y       +++  +  + D F+        YN+  N  N  ++N    +NN+ +  
Sbjct: 450 TPTSYSSSSNNNYDNNKYSSSSDDFSNKKFPEEGYNSNENQNNNNNDNKYFYNNNNKDSS 629

Query: 582 QEGEFASSSVKKLRKRQMRN---FFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYL 638
              +F       +  R   N   F+ +          +  + Y   +  NNN Y   NY 
Sbjct: 630 SNTKFTEEGYNSMENRNNNNNKYFYNNNNKDSSSNTKFPEEGYNSMQNQNNNNYEKYNYN 809

Query: 639 N 639
           N
Sbjct: 810 N 812


>TC87436 similar to PIR|T51575|T51575 2-hydroxyphytanoyl-CoA lyase-like
            protein - Arabidopsis thaliana, partial (75%)
          Length = 1592

 Score = 28.9 bits (63), Expect = 8.0
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = +1

Query: 680  RLQWHGHFPGKPDWSETSRFVAFTMVDSVKREI 712
            RL W  HF  +P WS+  +FV   +VD  K EI
Sbjct: 1015 RLNWLLHFGEEPKWSKDVKFV---LVDVSKEEI 1104


>TC91709 homologue to GP|21213868|emb|CAD12767. LHY protein {Phaseolus
           vulgaris}, partial (8%)
          Length = 649

 Score = 28.9 bits (63), Expect = 8.0
 Identities = 13/31 (41%), Positives = 17/31 (53%)
 Frame = -2

Query: 564 NGEDNNDGENHNNSWNCGQEGEFASSSVKKL 594
           N  +NN+  N+N  WNC       SSS KK+
Sbjct: 276 NNNNNNNNNNNNKDWNC-------SSSSKKI 205


>TC77271 similar to PIR|T07929|T07929 probable long-chain-fatty-acid--CoA
            ligase (EC 6.2.1.3) isoform 2 - rape, partial (94%)
          Length = 2486

 Score = 28.9 bits (63), Expect = 8.0
 Identities = 15/59 (25%), Positives = 29/59 (48%)
 Frame = -3

Query: 291  RVNFWGYSTVNYFSPMIRYSSAGIQNCGRDGINEVKFLIKEAHKRGIEVIMDVVFNHTV 349
            R+   GY+   +F PM +   AG+ N  ++G+  V       ++R + + +  VFN  +
Sbjct: 1917 RIEVHGYTI--FFCPMFKCWFAGVNNSNKEGLKTVSVNPYRFNRRNLAINILQVFNGNI 1747


>CA860191 homologue to GP|19697342|gb hypothetical protein {Dictyostelium
           discoideum}, partial (1%)
          Length = 504

 Score = 28.9 bits (63), Expect = 8.0
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = +2

Query: 557 NNKHNLPNGEDNNDGENHNNSWN 579
           NN +N  N  +NN+ +NH+N +N
Sbjct: 311 NNNNNNSNNNNNNNEDNHHNKFN 379


>BI262804 weakly similar to GP|11072027|gb F12A21.14 {Arabidopsis thaliana},
           partial (7%)
          Length = 653

 Score = 28.9 bits (63), Expect = 8.0
 Identities = 16/44 (36%), Positives = 19/44 (42%)
 Frame = -3

Query: 114 DFQDNQVTEYITLDPLMNKTGSVWHVFLKGDFGDMLYGYKFDGK 157
           +  D     YITL  L   T +      KG FG + YG   DGK
Sbjct: 270 NLMDEGTAYYITLSDLKVATNNFSKKIGKGSFGSVYYGKMKDGK 139


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.320    0.139    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,275,785
Number of Sequences: 36976
Number of extensions: 454888
Number of successful extensions: 2190
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 2117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2172
length of query: 791
length of database: 9,014,727
effective HSP length: 104
effective length of query: 687
effective length of database: 5,169,223
effective search space: 3551256201
effective search space used: 3551256201
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)