BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0587.7
(319 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8398 weakly similar to UP|Q84KK1 (Q84KK1) S haplotype-specific... 149 5e-37
TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromo... 81 3e-16
TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (... 67 4e-12
TC19187 64 4e-11
CB826878 62 1e-10
TC12990 57 5e-09
BP049904 54 5e-08
TC14801 51 2e-07
BP057238 49 1e-06
BG662160 47 4e-06
TC9055 42 2e-04
TC15119 41 2e-04
AI967342 30 0.001
BP050476 39 0.002
AW428730 36 0.008
AV777665 35 0.022
BP059894 33 0.064
BP038236 31 0.32
TC14451 homologue to GB|AAG40371.1|11762186|AF325019 AT4g27960 {... 30 0.54
TC13342 30 0.54
>TC8398 weakly similar to UP|Q84KK1 (Q84KK1) S haplotype-specific F-box
protein a, partial (9%)
Length = 1602
Score = 149 bits (377), Expect = 5e-37
Identities = 135/362 (37%), Positives = 169/362 (46%), Gaps = 52/362 (14%)
Frame = +1
Query: 10 HMQFLSPTNSSERSCYRFR*NLSSGLSACASTGNSSSLILNSSNFTFADLHL----TSQT 65
H QFLS TNSS +SC FR*+ G ACAS G+S S I NS N TF DL L TSQT
Sbjct: 67 HHQFLS-TNSSGKSCRCFR*SPLCGSGACASHGSSPSPIRNS*NCTFVDLQLKPQQTSQT 243
Query: 66 ---WRTPDYCM-----------ISIPTTTYPQMPG------------------LLCYSHL 93
W P + +S+ + P PG LL Y H
Sbjct: 244 AK*WS*PKETISHTVHYYPSSTLSMMKPSPPTNPGRSTPYANPSINHMSQSAMLLRYFHP 423
Query: 94 KTNMDIISIQ*RMSTV*LESATAWFLKSYGTL*SLFSSVQLVGFVHLFGSVSGTQLQGYG 153
KT *R V*LE ATAWFL S L + V+ TQL Y
Sbjct: 424 KT-----MTN*RAIVV*LEPATAWFL*S-------MKDTTLQSLQRNYHVVT*TQLPSYD 567
Query: 154 LDRPQL*I---SEIVLVRFLYSSFGFGYDCMNDTYKVVAVAVSCFREHKE---ERVVKVY 207
L QL + + L ++ F G D + + V R H E + V VY
Sbjct: 568 LKCHQLCLCVTNSFTL*SIVFFVF-LGLDMIL*MTPIKWWXVFYHRNHLEHCMQSVANVY 744
Query: 208 DMGGSFWRNIQSFP-VSLSH------ISQLGVYISGTLNWEVKTDNYTFIIVSLDLGTEE 260
+MG WR IQSFP + H Q GVY++GT+NW ++ N + I+SLDLG+E
Sbjct: 745 NMGDGCWRRIQSFPDFTREHWGRGTAPDQNGVYLNGTINWVLRL-NIAYDIISLDLGSEV 921
Query: 261 YSQLSLPYSPLYGEDFEF---PFLAVLKDCLCILENNEGDNRFLVWQMKKFGVHNSWIQL 317
S+LSLP P G + P L VLKD LC+L NN D F++W+M +FG H SW L
Sbjct: 922 CSRLSLPCFPPPGPKCVYDLPPILGVLKDSLCVLHNN-NDKTFVIWKMNEFGAHESWTPL 1098
Query: 318 YN 319
+N
Sbjct: 1099FN 1104
>TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromosome 5, TAC
clone:K14B20, partial (7%)
Length = 782
Score = 80.9 bits (198), Expect = 3e-16
Identities = 47/104 (45%), Positives = 60/104 (57%), Gaps = 1/104 (0%)
Frame = +3
Query: 175 GFGYDCMNDTYKVVAVAVSCFREHKEERVVKVYDMGGSFWRNIQSFPVSLSHISQLGVYI 234
GFGYDC +DTYKVV + +V VY+MG + WR IQ P + H+ VY+
Sbjct: 498 GFGYDCSSDTYKVVGMIPGL-------TMVNVYNMGDNCWRTIQISPHAPMHLQGSAVYV 656
Query: 235 SGTLNWEVKTDNYTFIIVSLDLGTEEYS-QLSLPYSPLYGEDFE 277
S TLNW T+ Y IVS DL EE + QLSLPY P ++F+
Sbjct: 657 SNTLNWLAGTNPY--FIVSFDLEKEECAQQLSLPYCPWRRKEFD 782
Score = 37.4 bits (85), Expect = 0.003
Identities = 31/74 (41%), Positives = 37/74 (49%), Gaps = 6/74 (8%)
Frame = +1
Query: 14 LSPTNSSERSCYRFR*NLSSGLSACASTGNSSSLILNSSNFT-FADLHLTSQTWRTPDY- 71
+S TNSS +S F *NLS C GN SSLIL+S N T F Q TP Y
Sbjct: 46 VSLTNSSLKSYLGFL*NLSCNSGWCPKHGNLSSLILSS*NCTFFTGCLFEMQILSTPAY* 225
Query: 72 ----CMISIPTTTY 81
MIS+ T++
Sbjct: 226 *NATPMISVDHTSH 267
>TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (SLF)-S4
protein, partial (10%)
Length = 551
Score = 67.0 bits (162), Expect = 4e-12
Identities = 37/82 (45%), Positives = 46/82 (55%)
Frame = +3
Query: 175 GFGYDCMNDTYKVVAVAVSCFREHKEERVVKVYDMGGSFWRNIQSFPVSLSHISQLGVYI 234
GFGYDC +DTYKVV + +V VY+MG + WR IQ P + H+ VY+
Sbjct: 327 GFGYDCSSDTYKVVGMIPGL-------TMVNVYNMGDNCWRTIQISPHAPMHLQGSAVYV 485
Query: 235 SGTLNWEVKTDNYTFIIVSLDL 256
S TLNW T+ Y IVS DL
Sbjct: 486 SNTLNWLAGTNPY--FIVSFDL 545
Score = 38.5 bits (88), Expect = 0.002
Identities = 32/79 (40%), Positives = 39/79 (48%), Gaps = 6/79 (7%)
Frame = +1
Query: 14 LSPTNSSERSCYRFR*NLSSGLSACASTGNSSSLILNSSNFT-FADLHLTSQTWRTPDY- 71
+S TNSS +S F *NLS C GN SSLIL+S N T F Q TP Y
Sbjct: 19 VSLTNSSLKSYLGFL*NLSCNSGWCPKHGNLSSLILSS*NCTFFTGCLFEMQILSTPAY* 198
Query: 72 ----CMISIPTTTYPQMPG 86
MIS+ T++ + G
Sbjct: 199*NATPMISVDHTSHLALSG 255
>TC19187
Length = 632
Score = 63.5 bits (153), Expect = 4e-11
Identities = 38/93 (40%), Positives = 55/93 (58%), Gaps = 6/93 (6%)
Frame = +1
Query: 231 GVYISGTLNWEVKTDNYT------FIIVSLDLGTEEYSQLSLPYSPLYGEDFEFPFLAVL 284
G ++SGTLNW NY+ +IIVSLDL E Y ++ P E+ P L+VL
Sbjct: 7 GKFVSGTLNWAA---NYSVGVSSLWIIVSLDLQKETYKEILPP--DYEKEECSTPTLSVL 171
Query: 285 KDCLCILENNEGDNRFLVWQMKKFGVHNSWIQL 317
K CLC+ +++ + F+VW MK +GV SW++L
Sbjct: 172 KGCLCMNYDHKRTD-FVVWMMKDYGVRESWVKL 267
>CB826878
Length = 635
Score = 62.4 bits (150), Expect = 1e-10
Identities = 30/74 (40%), Positives = 40/74 (53%)
Frame = +3
Query: 246 NYTFIIVSLDLGTEEYSQLSLPYSPLYGEDFEFPFLAVLKDCLCILENNEGDNRFLVWQM 305
NY I+ + S LP L P L VL+D LC+ +N++ F+VWQM
Sbjct: 285 NYNLIMQLGNTARCSVSGTQLPEQCLNSPHLYMPILGVLRDSLCVSQNDKKTRNFVVWQM 464
Query: 306 KKFGVHNSWIQLYN 319
K+FGVH SW QL+N
Sbjct: 465 KEFGVHKSWTQLFN 506
>TC12990
Length = 518
Score = 56.6 bits (135), Expect = 5e-09
Identities = 23/37 (62%), Positives = 29/37 (78%)
Frame = +1
Query: 283 VLKDCLCILENNEGDNRFLVWQMKKFGVHNSWIQLYN 319
VL CLCI +NN+ + F+VWQMK+FGVH SW QL+N
Sbjct: 4 VLGGCLCISQNNKETHSFVVWQMKEFGVHESWTQLFN 114
>BP049904
Length = 541
Score = 53.5 bits (127), Expect = 5e-08
Identities = 27/69 (39%), Positives = 40/69 (57%)
Frame = -3
Query: 251 IVSLDLGTEEYSQLSLPYSPLYGEDFEFPFLAVLKDCLCILENNEGDNRFLVWQMKKFGV 310
I SLDLG E Y + P YG + ++ L V +DCLCIL N++ +W MK++G
Sbjct: 521 IFSLDLGKETYEEF---LQPDYGIEVDYKILDVTRDCLCILANSD------IWVMKEYGN 369
Query: 311 HNSWIQLYN 319
SW +L++
Sbjct: 368 SESWTKLFS 342
>TC14801
Length = 595
Score = 51.2 bits (121), Expect = 2e-07
Identities = 24/56 (42%), Positives = 37/56 (65%), Gaps = 2/56 (3%)
Frame = +2
Query: 266 LPYSPLYGEDFEF--PFLAVLKDCLCILENNEGDNRFLVWQMKKFGVHNSWIQLYN 319
LPY P ++F+ L VL+ CLCI + ++ + F+V QMK++GV+ SW QL+N
Sbjct: 50 LPYCPWRRQEFDHFGSILGVLRGCLCISQYHKETHSFVVSQMKEYGVNESWTQLFN 217
>BP057238
Length = 434
Score = 48.9 bits (115), Expect = 1e-06
Identities = 40/105 (38%), Positives = 51/105 (48%), Gaps = 14/105 (13%)
Frame = -2
Query: 177 GYDCMNDTYK-VVAVAVSCFREHKEERVVKVYDMGGSFWRNIQSFPVSLSHISQL-GVYI 234
GYD DTYK V+A+ S E K E VY MG S WR I S P S + Q G ++
Sbjct: 433 GYDESRDTYKAVMALCHSIEPEPKME--TTVYCMGDSCWRKISSSPSSSVLLQQFDGQFV 260
Query: 235 SGTLNW----EVKTDNYTFIIVSLD--------LGTEEYSQLSLP 267
G +NW + NY + V+LD + E Y+ LSLP
Sbjct: 259 GGCVNWLALDNLNGPNYEWQSVTLDQLVIVCFHMREEAYTYLSLP 125
>BG662160
Length = 445
Score = 47.0 bits (110), Expect = 4e-06
Identities = 28/62 (45%), Positives = 34/62 (54%)
Frame = +3
Query: 8 LSHMQFLSPTNSSERSCYRFR*NLSSGLSACASTGNSSSLILNSSNFTFADLHLTSQTWR 67
+ H S NS +SC+ FR*NL C S G+ SS ILNSSN TF D L +T
Sbjct: 3 IRHEHSFSLKNSG*KSCHGFR*NLWCDSGVCLSHGSLSSPILNSSNSTFIDHLLQQETQI 182
Query: 68 TP 69
+P
Sbjct: 183SP 188
>TC9055
Length = 577
Score = 41.6 bits (96), Expect = 2e-04
Identities = 27/84 (32%), Positives = 43/84 (51%), Gaps = 2/84 (2%)
Frame = +2
Query: 236 GTLNWEV-KTDNYT-FIIVSLDLGTEEYSQLSLPYSPLYGEDFEFPFLAVLKDCLCILEN 293
G +NW K + + +IVSLD+ E Y LSLP + + VL +CLC+ +
Sbjct: 2 GCVNWLANKFEPFEQLVIVSLDMRQEAYRLLSLPQGT---SELPSAEIQVLGNCLCLFYD 172
Query: 294 NEGDNRFLVWQMKKFGVHNSWIQL 317
+ F+ W+M ++G+ SW L
Sbjct: 173 FK-RTHFVAWKMSEYGLPESWTPL 241
>TC15119
Length = 520
Score = 41.2 bits (95), Expect = 2e-04
Identities = 26/55 (47%), Positives = 32/55 (57%), Gaps = 2/55 (3%)
Frame = +3
Query: 265 SLPYSPLYGEDFEFPFLAVLKDCLCILENNEGDNRFLV--WQMKKFGVHNSWIQL 317
SLP + Y + E P L +LK+CLC + FLV WQM+KFGV SW QL
Sbjct: 3 SLPSNYDYEKHGE-PTLVILKNCLCAC------HEFLVTVWQMEKFGVRESWTQL 146
>AI967342
Length = 450
Score = 29.6 bits (65), Expect(2) = 0.001
Identities = 16/49 (32%), Positives = 28/49 (56%)
Frame = -3
Query: 229 QLGVYISGTLNWEVKTDNYTFIIVSLDLGTEEYSQLSLPYSPLYGEDFE 277
+ G+ ++G L+W V D F+I++ DL ++ LPY ++ ED E
Sbjct: 313 EAGLLLNGALHWLVY-DADNFVIIAFDLIGRSLFEIPLPYG-IHTEDIE 173
Score = 28.5 bits (62), Expect(2) = 0.001
Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = -1
Query: 281 LAVLKDCLCILENNEGDNRFL-VWQMKKF-GVH 311
L V+ DCLC+ N D +W MK++ G H
Sbjct: 162 LRVMGDCLCLCNPNSSDRAMAEIWMMKEYKGTH 64
>BP050476
Length = 520
Score = 38.5 bits (88), Expect = 0.002
Identities = 22/49 (44%), Positives = 29/49 (58%)
Frame = +2
Query: 173 SFGFGYDCMNDTYKVVAVAVSCFREHKEERVVKVYDMGGSFWRNIQSFP 221
+ GFGYD ++DTYKVV V +C ++ E V V D+ F R I S P
Sbjct: 383 NLGFGYDNLHDTYKVVLVHWNCTKQKMETMVYCVNDI---FCRKILSDP 520
>AW428730
Length = 405
Score = 36.2 bits (82), Expect = 0.008
Identities = 26/106 (24%), Positives = 49/106 (45%), Gaps = 7/106 (6%)
Frame = +3
Query: 174 FGFGYDCMNDTYKVVAVAVSCFREHKEERVVKVYDMGGSFWRNIQSFPVSLSHISQL--- 230
FGFGYD D Y VV V V+ + V+ + + + W+ + V L ++ +
Sbjct: 42 FGFGYDSSTDDYLVVRVPVTDCYQPTHLPDVQFFSLRANMWKYTEG--VDLPPLTTIDLC 215
Query: 231 -GVYISGTLNWEVK--TDNYT-FIIVSLDLGTEEYSQLSLPYSPLY 272
G+ + ++W V D T I++ DL + ++ +P+ L+
Sbjct: 216 HGLLFNEAIHWAVSNWVDGVTNMFIIAFDLMEKRLLEIPMPHGLLF 353
>AV777665
Length = 425
Score = 34.7 bits (78), Expect = 0.022
Identities = 29/79 (36%), Positives = 40/79 (49%), Gaps = 15/79 (18%)
Frame = +1
Query: 255 DLGTEEYSQLSLPYSPLYGED----FEFPF-----LAVLKDCLCIL------ENNEGDNR 299
DLG E ++S P YG D + P L VL+DCL I+ E+NE D
Sbjct: 1 DLGEESCQEIS---QPNYGVDSSVFVDHPDMFCLKLEVLRDCLSIIVHVRDFEDNE-DYE 168
Query: 300 FLVWQMKKFGVHNSWIQLY 318
+L W MK++G SW +L+
Sbjct: 169 YL-WVMKEYGNRESWTKLF 222
>BP059894
Length = 474
Score = 33.1 bits (74), Expect = 0.064
Identities = 17/45 (37%), Positives = 28/45 (61%), Gaps = 4/45 (8%)
Frame = -3
Query: 277 EFPF--LAVLKDCLCILENNEGDNR--FLVWQMKKFGVHNSWIQL 317
E P+ + VL +CLC+ + DNR F+ W+M ++GV SW ++
Sbjct: 451 ELPYVEIQVLGNCLCLFHD---DNRTHFVGWKMTEYGVPESWTRM 326
>BP038236
Length = 515
Score = 30.8 bits (68), Expect = 0.32
Identities = 15/37 (40%), Positives = 22/37 (58%)
Frame = -1
Query: 283 VLKDCLCILENNEGDNRFLVWQMKKFGVHNSWIQLYN 319
VL+DCL +L N VW MK++G SW +L++
Sbjct: 515 VLRDCLSMLSGNY--QVCDVWLMKEYGNQQSWTKLFS 411
>TC14451 homologue to GB|AAG40371.1|11762186|AF325019 AT4g27960 {Arabidopsis
thaliana;} , partial (84%)
Length = 997
Score = 30.0 bits (66), Expect = 0.54
Identities = 10/24 (41%), Positives = 19/24 (78%)
Frame = -1
Query: 206 VYDMGGSFWRNIQSFPVSLSHISQ 229
++++GGSFWR++ SF + L I++
Sbjct: 337 LHEVGGSFWRSLSSFKIRLDAIAK 266
>TC13342
Length = 549
Score = 30.0 bits (66), Expect = 0.54
Identities = 16/74 (21%), Positives = 36/74 (48%), Gaps = 6/74 (8%)
Frame = +2
Query: 173 SFGFGYDCMNDTYKVVAVAVSCFREHKE---ERVVKVYDMGGSFWRN---IQSFPVSLSH 226
++G G+D + YKV+ + F + +++Y + + WRN + S P+ +
Sbjct: 263 AYGLGFDLKTNYYKVIVIKDLWFENAER*IGNWTLELYSLNFNSWRNQIVVLSPPI*IWS 442
Query: 227 ISQLGVYISGTLNW 240
S++ Y++ +W
Sbjct: 443 SSEVYTYVNHCCHW 484
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.330 0.141 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,549,875
Number of Sequences: 28460
Number of extensions: 95903
Number of successful extensions: 605
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 585
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 591
length of query: 319
length of database: 4,897,600
effective HSP length: 90
effective length of query: 229
effective length of database: 2,336,200
effective search space: 534989800
effective search space used: 534989800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0587.7