BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0550.6
(680 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP084855 113 1e-25
BP033881 94 9e-20
BP070082 85 3e-17
TC11308 similar to UP|CENB_HUMAN (P07199) Major centromere autoa... 34 0.068
TC13221 similar to UP|PPAN_ARATH (Q9ASU7) Peter Pan-like protein... 33 0.15
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 33 0.20
TC12505 similar to UP|BAD00698 (BAD00698) Sericin 1A, partial (5%) 32 0.34
AV418706 32 0.34
AV406990 32 0.34
TC14984 similar to GB|AAM62447.1|21553354|AY085214 glycine-rich ... 32 0.44
TC16191 similar to GB|AAO39946.1|28372928|BT003718 At1g49880 {Ar... 31 0.57
TC14455 homologue to UP|CAC83767 (CAC83767) Allene oxide cyclase... 31 0.75
TC19732 weakly similar to UP|Q9SIP8 (Q9SIP8) At2g31600 protein, ... 31 0.75
TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, pa... 30 0.98
TC13834 homologue to UP|Q948R9 (Q948R9) RPS6-like protein (AT3g1... 30 0.98
BP034655 30 1.3
CB827268 30 1.3
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 30 1.3
TC13588 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall... 27 1.6
TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) prec... 30 1.7
>BP084855
Length = 373
Score = 113 bits (282), Expect = 1e-25
Identities = 57/83 (68%), Positives = 68/83 (81%), Gaps = 1/83 (1%)
Frame = -2
Query: 599 QISVVEPGFDLSRIGWLKEIKDGQVVGDDDISLDLLPQFDDESEPEEDG-EDGNEQHRNE 657
QISVVEP FDLS IG+LKEIKDGQVVGDDD+ +DLLPQF+ ESE EE+ EDG+ + +N
Sbjct: 372 QISVVEPDFDLSCIGFLKEIKDGQVVGDDDVDIDLLPQFNSESEYEEEKCEDGHGEDKNG 193
Query: 658 DQEKEDPQAGTSQGNNANNENLA 680
D+ + DP+A SQ NNANNENLA
Sbjct: 192 DEGENDPKAAASQDNNANNENLA 124
>BP033881
Length = 528
Score = 93.6 bits (231), Expect = 9e-20
Identities = 47/63 (74%), Positives = 54/63 (85%)
Frame = +2
Query: 1 MSLPNASLSSLELSSPSTEEEVVAPSIIEIHSSPSTHDDSGPAGSVDSILSSNGDLSSPE 60
MSL + S+SSLELSSPSTE + APS+IEI SSPS H+DSGPA SVDS+LSSNGDLSS E
Sbjct: 329 MSLNSFSVSSLELSSPSTEGGIYAPSVIEIQSSPSIHEDSGPADSVDSVLSSNGDLSSXE 508
Query: 61 WRS 63
WR+
Sbjct: 509 WRT 517
>BP070082
Length = 489
Score = 85.1 bits (209), Expect = 3e-17
Identities = 39/54 (72%), Positives = 48/54 (88%)
Frame = -1
Query: 589 YDQGFLTAKEQISVVEPGFDLSRIGWLKEIKDGQVVGDDDISLDLLPQFDDESE 642
Y +GF+ AKEQ+SVV+P FDLSRIG+LK+I+DGQV+G DDI LDLLP+FD ESE
Sbjct: 345 YVEGFVAAKEQVSVVDPDFDLSRIGFLKKIQDGQVIGHDDIDLDLLPKFDSESE 184
>TC11308 similar to UP|CENB_HUMAN (P07199) Major centromere autoantigen B
(Centromere protein B) (CENP-B), partial (4%)
Length = 756
Score = 34.3 bits (77), Expect = 0.068
Identities = 17/40 (42%), Positives = 22/40 (54%)
Frame = -2
Query: 625 GDDDISLDLLPQFDDESEPEEDGEDGNEQHRNEDQEKEDP 664
GD+D S + D+E E E + E+G E ED E EDP
Sbjct: 350 GDEDGSRFHEIENDNEGEEEVEAEEGEEDEEEEDDEDEDP 231
Score = 27.7 bits (60), Expect = 6.4
Identities = 12/55 (21%), Positives = 23/55 (41%)
Frame = -2
Query: 623 VVGDDDISLDLLPQFDDESEPEEDGEDGNEQHRNEDQEKEDPQAGTSQGNNANNE 677
V+GDD + ++ + E EDG+ H E+ + + + +G E
Sbjct: 419 VIGDDGAETTSVDPVEENARAEPGDEDGSRFHEIENDNEGEEEVEAEEGEEDEEE 255
>TC13221 similar to UP|PPAN_ARATH (Q9ASU7) Peter Pan-like protein, partial
(15%)
Length = 521
Score = 33.1 bits (74), Expect = 0.15
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = +3
Query: 638 DDESEPEEDGEDGNEQHRNEDQEKEDPQAGTSQGNN 673
DDE + EDGED +E +EDQ+ EDP+ + + ++
Sbjct: 183 DDEMQDSEDGED-SEGSEDEDQDGEDPEEASLEDDD 287
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 32.7 bits (73), Expect = 0.20
Identities = 14/50 (28%), Positives = 27/50 (54%), Gaps = 10/50 (20%)
Frame = -2
Query: 639 DESEPEEDGEDGNEQHRNEDQEKEDP----------QAGTSQGNNANNEN 678
++ + + +GEDGN+ +ED + + P + G GNN+NN++
Sbjct: 641 EDKQHDAEGEDGNDDEGDEDDDGDGPFGEGEEDLSSEDGAGYGNNSNNKS 492
Score = 30.8 bits (68), Expect = 0.75
Identities = 21/53 (39%), Positives = 31/53 (57%), Gaps = 2/53 (3%)
Frame = -2
Query: 616 KEIKDGQVVGDDDISLDLLPQFDDESEPEEDGEDGNEQHRNEDQEK--EDPQA 666
+E +DG+ DDD + + DD E EE E+G E+ NED+E+ ED +A
Sbjct: 425 EEDEDGEDQEDDDDDDEDDDEEDDGGEDEE--EEGVEEEDNEDEEEDEEDEEA 273
Score = 29.6 bits (65), Expect = 1.7
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -2
Query: 625 GDDDISLDLLPQFDDESEPEEDGE--DGNEQHRNEDQEKED 663
G+D+ D Q DD+ + E+D E DG E E E+ED
Sbjct: 434 GEDEEDEDGEDQEDDDDDDEDDDEEDDGGEDEEEEGVEEED 312
Score = 28.1 bits (61), Expect = 4.9
Identities = 10/36 (27%), Positives = 20/36 (54%)
Frame = -2
Query: 635 PQFDDESEPEEDGEDGNEQHRNEDQEKEDPQAGTSQ 670
P+ + E E +EDGED + +++ + E+ G +
Sbjct: 446 PEENGEDEEDEDGEDQEDDDDDDEDDDEEDDGGEDE 339
Score = 27.7 bits (60), Expect = 6.4
Identities = 12/44 (27%), Positives = 25/44 (56%), Gaps = 4/44 (9%)
Frame = -2
Query: 638 DDESEPEEDGEDGNEQHRNEDQE----KEDPQAGTSQGNNANNE 677
D+E E ED ED ++ ++D+E +++ + G + +N + E
Sbjct: 428 DEEDEDGEDQEDDDDDDEDDDEEDDGGEDEEEEGVEEEDNEDEE 297
>TC12505 similar to UP|BAD00698 (BAD00698) Sericin 1A, partial (5%)
Length = 569
Score = 32.0 bits (71), Expect = 0.34
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = -3
Query: 617 EIKDGQVVGDDDISLDLLPQFDDESEPEEDGEDGNEQHRNEDQEKEDPQAGTSQGNNAN 675
E ++ + V DDD + +LP S P+ +D + ED++ ED A + A+
Sbjct: 483 EEEEEEEVDDDDDDVQVLPAAATSSHPKRKRDDDADDREEEDEDDEDVVAFSKPSQEAS 307
>AV418706
Length = 374
Score = 32.0 bits (71), Expect = 0.34
Identities = 26/85 (30%), Positives = 33/85 (38%), Gaps = 3/85 (3%)
Frame = +1
Query: 335 SAAAAKKRAAETEQKKKNEGTSGSDNVRDPKRQKTSSA---AGGRPLHQSTLDPRSHPAE 391
+AAAA AA + + +S S + P TSS A P S P + P
Sbjct: 136 AAAAATVAAAHSSAGSSSSSSSSSSSSAPPAPSSTSSTTLNALPSPSRHSNFPPSNSPPP 315
Query: 392 KKKGHDNVPPPQQDSSALINRPPTP 416
PPP SS + PPTP
Sbjct: 316 --------PPPSPPSSNSHSPPPTP 366
>AV406990
Length = 392
Score = 32.0 bits (71), Expect = 0.34
Identities = 21/75 (28%), Positives = 29/75 (38%)
Frame = +1
Query: 367 QKTSSAAGGRPLHQSTLDPRSHPAEKKKGHDNVPPPQQDSSALINRPPTPFNQAGPSLAI 426
QKT AG P P +HP H ++ + PP P +++
Sbjct: 118 QKTEGEAGASP----KTAPEAHPPISHSHHST-------TNLTLPPPPLPSQTPWMQISM 264
Query: 427 GGEAPPPLLNLSDPH 441
+PPPLL L PH
Sbjct: 265 PSPSPPPLLPLLRPH 309
>TC14984 similar to GB|AAM62447.1|21553354|AY085214 glycine-rich RNA binding
protein 7 {Arabidopsis thaliana;} , partial (19%)
Length = 483
Score = 31.6 bits (70), Expect = 0.44
Identities = 18/43 (41%), Positives = 22/43 (50%)
Frame = +3
Query: 401 PPQQDSSALINRPPTPFNQAGPSLAIGGEAPPPLLNLSDPHFN 443
PP ++A RPP P Q P A+ PPPL + S PH N
Sbjct: 18 PPATPTTADGRRPPPPSTQPPPPGAL--RPPPPLHSPSRPHHN 140
>TC16191 similar to GB|AAO39946.1|28372928|BT003718 At1g49880 {Arabidopsis
thaliana;}, partial (60%)
Length = 587
Score = 31.2 bits (69), Expect = 0.57
Identities = 16/46 (34%), Positives = 23/46 (49%), Gaps = 6/46 (13%)
Frame = +2
Query: 150 FSPFICHVLSFLN------VAPCQLQPNAWGFLRCFEILCEHLSFT 189
FS ++CHV + +N V PC+ WG L C + CE + T
Sbjct: 269 FSHWLCHVHNVVNRSLGKPVFPCERVDARWGKLECEQKACEIIGST 406
>TC14455 homologue to UP|CAC83767 (CAC83767) Allene oxide cyclase precursor
, partial (79%)
Length = 990
Score = 30.8 bits (68), Expect = 0.75
Identities = 26/90 (28%), Positives = 41/90 (44%), Gaps = 14/90 (15%)
Frame = +2
Query: 236 KYFKVMESPEIKNLFRDSDNNPLFPFYWTKN---------PRRKISVSYDALDESEQGVA 286
K FK+ SP++ + R S N F+++ N P + +S ++E ++G
Sbjct: 152 KSFKISASPQVPSTKRSSKNTTTTAFFFSNNSKQDHDSSRPAKVQELSVYEINERDRGSP 331
Query: 287 DYLR--TLPVISGHDLIEASK---SGTLNQ 311
YLR PV S DL+ S SG L +
Sbjct: 332 AYLRLSQKPVNSLGDLVPFSNKLYSGNLEK 421
>TC19732 weakly similar to UP|Q9SIP8 (Q9SIP8) At2g31600 protein, partial
(24%)
Length = 387
Score = 30.8 bits (68), Expect = 0.75
Identities = 17/61 (27%), Positives = 28/61 (45%), Gaps = 5/61 (8%)
Frame = +2
Query: 358 SDNVRDPKRQKTSSAA-----GGRPLHQSTLDPRSHPAEKKKGHDNVPPPQQDSSALINR 412
SD+ P R ++A+ G R + H ++GH + PPP + + AL+ R
Sbjct: 26 SDSANQPPRHVRTTASSAISDGHRRRRPRRRASKLHRPIPERGHRSAPPPSETTRALLQR 205
Query: 413 P 413
P
Sbjct: 206 P 208
>TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, partial (3%)
Length = 504
Score = 30.4 bits (67), Expect = 0.98
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = +3
Query: 626 DDDISLDLLPQFDDESEPEEDGEDGNEQHRNEDQEKEDPQAGTSQGNNANNENLA 680
DDD D D ESE EED +D ++D + EDP G G +++++++
Sbjct: 57 DDDSDPD-----DAESEDEEDDDD------DDDGDDEDPLLGPGLGRESDSDDMS 188
>TC13834 homologue to UP|Q948R9 (Q948R9) RPS6-like protein
(AT3g17170/K14A17_29), partial (13%)
Length = 517
Score = 30.4 bits (67), Expect = 0.98
Identities = 16/44 (36%), Positives = 26/44 (58%), Gaps = 2/44 (4%)
Frame = +1
Query: 626 DDDISLDLLPQFDDESEPEE--DGEDGNEQHRNEDQEKEDPQAG 667
DDD +L +DD+ + EE DGEDG+ + D +++D +G
Sbjct: 151 DDDDDEELDEDYDDDWDGEEDADGEDGDAEFIVIDDDQDDVDSG 282
>BP034655
Length = 517
Score = 30.0 bits (66), Expect = 1.3
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +1
Query: 624 VGDDDISLDLLPQFDDESEPEEDGEDGNEQHRNEDQEKED 663
+ DDD + + ++E E EE+ E+ E+ +D+E ED
Sbjct: 130 LSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDEEDED 249
Score = 27.3 bits (59), Expect = 8.3
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +1
Query: 627 DDISLDLLPQFDDESEPEEDGEDGNEQHRNEDQEKEDPQAGTSQGNNANNENL 679
D + + L+P + ++ E EDGN++ R E+ E G SQGN ++
Sbjct: 316 DSVEVILIPDSEQQNYSE---EDGNKRRRIEEGEAPTSSLG-SQGNELTGTDI 462
>CB827268
Length = 533
Score = 30.0 bits (66), Expect = 1.3
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +2
Query: 376 RPLHQSTLDPRSHPAEKKKGHDNVPPPQQDSSALINRPPTPFNQAGPSLAIGGEAPPPLL 435
RP + S L+PR+H H P PQ++ PTP + P L PP L
Sbjct: 134 RPANHSPLEPRTH------AHPTSPHPQRNPQC--KSTPTPDLKPNPPLCSAVVTQPPDL 289
Query: 436 NL 437
L
Sbjct: 290 KL 295
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 30.0 bits (66), Expect = 1.3
Identities = 10/27 (37%), Positives = 20/27 (74%)
Frame = +2
Query: 637 FDDESEPEEDGEDGNEQHRNEDQEKED 663
F+D E +EDG++ ++ ++D+E+ED
Sbjct: 761 FEDIEEDDEDGDEDEDEDDDDDEEEED 841
Score = 28.9 bits (63), Expect = 2.9
Identities = 28/137 (20%), Positives = 54/137 (38%), Gaps = 13/137 (9%)
Frame = +2
Query: 545 LLQKTKSLKEAINDKHTAIQAKYQKLEKKYDRLNASIIGRASLQYDQGFLTAKEQISVVE 604
L QK K K+T K +K E ++ N + D +E +++E
Sbjct: 500 LTQKILKKKPKKGSKNTKPITKTEKCESFFNFFNPPQVPEDDDDIDDD--AVEELQNLME 673
Query: 605 PGFDLSR----------IGWLK---EIKDGQVVGDDDISLDLLPQFDDESEPEEDGEDGN 651
+D+ + W E D + + +DD D DD+ + EE+ +D +
Sbjct: 674 HDYDIGSTIRDKIIPHAVSWFTGEAEQSDFEDIEEDDEDGDEDEDEDDDDDEEEEDDDDD 853
Query: 652 EQHRNEDQEKEDPQAGT 668
++ E + K ++G+
Sbjct: 854 DEDDEEGEGKSKSKSGS 904
>TC13588 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall structural
protein 1.8 precursor (GRP 1.8), partial (6%)
Length = 517
Score = 26.9 bits (58), Expect(2) = 1.6
Identities = 18/71 (25%), Positives = 23/71 (32%)
Frame = -2
Query: 346 TEQKKKNEGTSGSDNVRDPKRQKTSSAAGGRPLHQSTLDPRSHPAEKKKGHDNVPPPQQD 405
T+ N S S DP ++ RP+ P HP H + P Q
Sbjct: 471 TQPNNFNSSFSSSAMPVDPAAAAATTTTASRPI-----SPLPHPHPHAHHHHHYQSPPQQ 307
Query: 406 SSALINRPPTP 416
L R P P
Sbjct: 306 QQTLPIRAPNP 274
Score = 21.2 bits (43), Expect(2) = 1.6
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -3
Query: 414 PTPFNQAGPSLAIGGEAPPPLL 435
P P A PSL G + PP+L
Sbjct: 221 PLPPAAAAPSLPKGLDPSPPIL 156
>TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) precursor,
partial (51%)
Length = 1566
Score = 29.6 bits (65), Expect = 1.7
Identities = 19/63 (30%), Positives = 29/63 (45%), Gaps = 19/63 (30%)
Frame = +1
Query: 624 VGDDDISLDLLPQFDDESEPEEDGEDGNEQH-------------------RNEDQEKEDP 664
V DD+S + P+ DE E EED ++ EQH +ED+++E+
Sbjct: 463 VEGDDLSF-ISPESADEDEDEEDEDEDQEQHGRHSRPSGRRTRRPSREEDEDEDEDEEER 639
Query: 665 QAG 667
Q G
Sbjct: 640 QGG 648
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.131 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,910,324
Number of Sequences: 28460
Number of extensions: 198218
Number of successful extensions: 1216
Number of sequences better than 10.0: 77
Number of HSP's better than 10.0 without gapping: 1132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1185
length of query: 680
length of database: 4,897,600
effective HSP length: 96
effective length of query: 584
effective length of database: 2,165,440
effective search space: 1264616960
effective search space used: 1264616960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0550.6