BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0550.4
(888 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17840 similar to UP|LON1_SPIOL (O04979) Lon protease homolog 1... 360 e-100
AV418083 179 2e-45
AV777729 126 2e-29
TC8836 similar to UP|LON1_ARATH (O64948) Lon protease homolog 1,... 118 5e-27
TC18063 similar to UP|LON2_ARATH (P93655) Lon protease homolog 2... 67 1e-11
TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J... 39 0.004
TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), pa... 35 0.068
TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {... 34 0.12
TC12787 weakly similar to UP|MSP1_YEAST (P28737) MSP1 protein (T... 33 0.15
TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase... 33 0.20
TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%) 33 0.26
CB826675 32 0.34
TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 32 0.34
AV408284 32 0.44
TC16655 homologue to UP|O99018 (O99018) Chloroplast protease pre... 32 0.58
TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPas... 31 0.99
CB829689 30 1.3
BP046346 30 2.2
TC13343 similar to UP|Q9FMR9 (Q9FMR9) Ruv DNA-helicase-like prot... 30 2.2
BP040113 30 2.2
>TC17840 similar to UP|LON1_SPIOL (O04979) Lon protease homolog 1,
mitochondrial precursor , partial (22%)
Length = 816
Score = 360 bits (923), Expect = e-100
Identities = 189/223 (84%), Positives = 200/223 (88%), Gaps = 2/223 (0%)
Frame = +3
Query: 1 MAESVELPSRLAILPFRNKVLLPGAIIRIRCTSPTSVKLVEQELWQREDKGLIGILPVRD 60
MAESVELPSRLAILPFRNKVLLPGAIIRIRCTSP+SVK VEQEL QRE+KGLIGILPVRD
Sbjct: 150 MAESVELPSRLAILPFRNKVLLPGAIIRIRCTSPSSVKWVEQELRQREEKGLIGILPVRD 329
Query: 61 AAETEVKPVGPTISQDGDT--LDQSSKVHGASSDSHKLDAKAQNDVVHWHNRGVAARALH 118
AA E+K +GPT+SQ T LDQSSKV SSDSHK D K QND VHWHNRGVAARALH
Sbjct: 330 AAAAEIKQLGPTVSQGAGTDFLDQSSKVKDGSSDSHKADTKKQNDGVHWHNRGVAARALH 509
Query: 119 LSRGVEKPSGRVTYIVVLEGLCRFSVQELSTRGTYHTARISSLEMNKTEMEQVEHDPDFI 178
LSRGVEKPSGRVTYIVVLEGLCRFSV+EL+TRG YHTARISSLE KTEMEQVE DPDFI
Sbjct: 510 LSRGVEKPSGRVTYIVVLEGLCRFSVEELNTRGIYHTARISSLE-TKTEMEQVEQDPDFI 686
Query: 179 MLSRQFKATAMELISVLEQKQKTGGRTKVLLETVPVHKLADIF 221
+LSR+FKATAMELISVLE KQKTGGRTK+LL+ VPVHKLADIF
Sbjct: 687 LLSRRFKATAMELISVLELKQKTGGRTKLLLDNVPVHKLADIF 815
>AV418083
Length = 281
Score = 179 bits (454), Expect = 2e-45
Identities = 86/92 (93%), Positives = 90/92 (97%)
Frame = +2
Query: 482 GSDVRGDPASALLEVLDPEQNKTFNDHYLNVPFDLSKVIFVATANRAQQIPPPLLDRMEV 541
GSD+RGDPASALLEVLDPEQNKTFNDHYLNVPFDLSKV+FVATANR Q IPPPLLDRMEV
Sbjct: 5 GSDIRGDPASALLEVLDPEQNKTFNDHYLNVPFDLSKVVFVATANRMQPIPPPLLDRMEV 184
Query: 542 IELPGYTPEEKLKIAMKHLIPRVLDQHGLSSE 573
IELPGYTPEEKL+IAM+HLIPRVLDQHGLSSE
Sbjct: 185 IELPGYTPEEKLQIAMRHLIPRVLDQHGLSSE 280
>AV777729
Length = 371
Score = 126 bits (316), Expect = 2e-29
Identities = 61/119 (51%), Positives = 82/119 (68%)
Frame = -3
Query: 487 GDPASALLEVLDPEQNKTFNDHYLNVPFDLSKVIFVATANRAQQIPPPLLDRMEVIELPG 546
GDPASALLE+LDPEQN F DHYL+VP DLSKV+FV TAN + IP PLLDRMEVI + G
Sbjct: 360 GDPASALLELLDPEQNANFLDHYLDVPIDLSKVLFVCTANVVEMIPNPLLDRMEVISIAG 181
Query: 547 YTPEEKLKIAMKHLIPRVLDQHGLSSEYLQIPEGMVQLVIQRYTREAGVRNLERNLASL 605
Y +EK+ IA +L + G+ E +++ + + +I Y RE+GVRNL++ + +
Sbjct: 180 YITDEKMHIARDYLEKSTREACGIKPEQVEVTDAALLALIDNYCRESGVRNLQKQIEKI 4
>TC8836 similar to UP|LON1_ARATH (O64948) Lon protease homolog 1,
mitochondrial precursor , partial (7%)
Length = 587
Score = 118 bits (295), Expect = 5e-27
Identities = 57/67 (85%), Positives = 61/67 (90%)
Frame = +3
Query: 822 GGIKDKILAAHRCGIKRVIIPERNLKDLVEVPSSVLVNLEILLAKRMEDVLEQALEGGCP 881
GG+KDKILAAHR GIKRVI+PERNLKDLVEVPSSVL NLEILLAKR+EDVLE A +GGCP
Sbjct: 3 GGVKDKILAAHRYGIKRVILPERNLKDLVEVPSSVLANLEILLAKRVEDVLEHAFDGGCP 182
Query: 882 WRHQSKL 888
WR SKL
Sbjct: 183 WRQNSKL 203
>TC18063 similar to UP|LON2_ARATH (P93655) Lon protease homolog 2,
mitochondrial precursor , partial (9%)
Length = 492
Score = 67.4 bits (163), Expect = 1e-11
Identities = 30/77 (38%), Positives = 48/77 (61%)
Frame = +3
Query: 799 KKRVRSDTAMTGEMTLRGLVLPVGGIKDKILAAHRCGIKRVIIPERNLKDLVEVPSSVLV 858
KK V+ D AMTGE+TL G +LP+GG+K+K +AA R G+K ++ P N +D E+ +V
Sbjct: 3 KKPVKKDLAMTGEVTLTGKILPIGGVKEKTIAARRSGVKTIVFPSANRRDYDELAPNVKE 182
Query: 859 NLEILLAKRMEDVLEQA 875
L++ + + + A
Sbjct: 183 GLDVHFVDDFKQIFDLA 233
>TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J8.110 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 771
Score = 38.9 bits (89), Expect = 0.004
Identities = 43/173 (24%), Positives = 76/173 (43%), Gaps = 7/173 (4%)
Frame = +3
Query: 396 LKPDARGPVLCFVGPPGVGKTSLASSIAAALDRKFVRISLGGV------KDEADIRGHRR 449
LKP RG +L GPPG GKT LA +IA F+ +S+ + +DE ++R
Sbjct: 294 LKP-CRGILL--FGPPGTGKTMLAXAIANEAGASFINVSMSTITSKWFGEDEKNVRA--- 455
Query: 450 TYIGSMPGRLIDGLKRVAVCNPVMLLDEIDK-TGSDVRGDPASALLEVLDPEQNKTFNDH 508
+ S+ + V ++ +DE+D G R A+ ++ + T D
Sbjct: 456 --LFSLAAK---------VAPTIIFVDEVDSMLGQRTRVGEHEAMRKI--KNEFMTHWDG 596
Query: 509 YLNVPFDLSKVIFVATANRAQQIPPPLLDRMEVIELPGYTPEEKLKIAMKHLI 561
L P + +++ +A NR + ++ R E + G E ++ +K L+
Sbjct: 597 LLTAPGE--QILVLAATNRPFDLDEAIIRRFERRIMVGLPSVENREMILKTLL 749
>TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), partial (32%)
Length = 1418
Score = 34.7 bits (78), Expect = 0.068
Identities = 37/124 (29%), Positives = 52/124 (41%), Gaps = 8/124 (6%)
Frame = +2
Query: 379 GLAKVKQRIIEYLAVRKLKPDA-------RGPVLCFVGPPGVGKTSLASSIAAALDRKFV 431
GL VK+ I++ + + L D R VL + GPPG GKT LA ++A F+
Sbjct: 236 GLEDVKKSILDTVQLPLLHKDLFASGLRKRSGVLLY-GPPGTGKTLLAKAVATECSLNFL 412
Query: 432 RISLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVAVCNP-VMLLDEIDKTGSDVRGDPA 490
VK I YIG + D ++ P V+ DE+D PA
Sbjct: 413 -----SVKGPELI----NMYIGESEKNVRDIFQKARSARPCVIFFDELDSLA------PA 547
Query: 491 SALL 494
S +L
Sbjct: 548 SGML 559
>TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {Arabidopsis
thaliana;}, partial (87%)
Length = 1385
Score = 33.9 bits (76), Expect = 0.12
Identities = 26/77 (33%), Positives = 38/77 (48%), Gaps = 2/77 (2%)
Frame = +3
Query: 409 GPPGVGKTSLASSIAAALDRKFVRISLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVA- 467
GPPG GKT LA ++A D F+R+S + + YIG R++ L +A
Sbjct: 450 GPPGTGKTLLARAVAHHTDCTFIRVSGSELVQK---------YIGE-GSRMVRELFVMAR 599
Query: 468 -VCNPVMLLDEIDKTGS 483
++ +DEID GS
Sbjct: 600 EHAPSIIFMDEIDSIGS 650
>TC12787 weakly similar to UP|MSP1_YEAST (P28737) MSP1 protein (TAT-binding
homolog 4), partial (20%)
Length = 389
Score = 33.5 bits (75), Expect = 0.15
Identities = 13/30 (43%), Positives = 18/30 (59%)
Frame = +3
Query: 409 GPPGVGKTSLASSIAAALDRKFVRISLGGV 438
GPPG GKT LA ++A F+ IS+ +
Sbjct: 102 GPPGTGKTMLAKAVATEAGANFINISMSSI 191
>TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase subunit
RPT2a, partial (69%)
Length = 916
Score = 33.1 bits (74), Expect = 0.20
Identities = 26/77 (33%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
Frame = +1
Query: 409 GPPGVGKTSLASSIAAALDRKFVRISLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVA- 467
G PG GKT LA ++A + F+R+ V E + Y+G P +L+ L RVA
Sbjct: 505 GEPGTGKTLLAKAVANSTSATFLRV----VGSEL-----IQKYLGDGP-KLVRELFRVAD 654
Query: 468 -VCNPVMLLDEIDKTGS 483
+ ++ +DEID G+
Sbjct: 655 DLSPSIVFIDEIDAVGT 705
>TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%)
Length = 532
Score = 32.7 bits (73), Expect = 0.26
Identities = 46/173 (26%), Positives = 75/173 (42%), Gaps = 6/173 (3%)
Frame = +1
Query: 409 GPPGVGKTSLASSIAAALDRKFVRISLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVAV 468
GPPG GKT LA ++A F IS V + RG + +++ L R
Sbjct: 55 GPPGTGKTMLAKAVATECKTTFFNISASSVVSK--WRGDSEKLV-----KVLFQLARHHA 213
Query: 469 CNPVMLLDEIDKTGSDVRGDPASALLEVLDPEQNKTFNDHYLNVPFDLSK----VIFVAT 524
+ + LDEID S RG+ S + E ++ L L++ V +A
Sbjct: 214 PSTI-FLDEIDAIISQ-RGEARS------EHEASRRLKTELLIQMDGLTRTDELVFVLAA 369
Query: 525 ANRAQQIPPPLLDRMEV-IELPGYTPEEKLKIAM-KHLIPRVLDQHGLSSEYL 575
N ++ +L R+E I +P PE + ++AM + L+P D+ + + L
Sbjct: 370 TNLPWELDAAMLRRLEKRILVP--LPEPEARVAMFEELLPPQPDEESIPYDLL 522
>CB826675
Length = 551
Score = 32.3 bits (72), Expect = 0.34
Identities = 34/127 (26%), Positives = 57/127 (44%), Gaps = 13/127 (10%)
Frame = +1
Query: 375 SDHYGLAKVKQRIIEYLAV------RKLKPDARGPV-LCFVGPPGVGKTSLASSIAAALD 427
+D GL K Q ++E + + R K R P + GPPG GKT +A + AA +
Sbjct: 136 NDVGGLEKQIQELVEAIVLPMTHKERFQKLGVRPPKGVLLYGPPGTGKTLMARACAAQTN 315
Query: 428 RKFVRISLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVAVCNP-VMLLDEIDKTG---- 482
F++++ + + +IG + D + +P ++ +DEID G
Sbjct: 316 ATFLKLAGPQLV---------QMFIGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRF 468
Query: 483 -SDVRGD 488
S+V GD
Sbjct: 469 DSEVSGD 489
>TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (65%)
Length = 901
Score = 32.3 bits (72), Expect = 0.34
Identities = 24/73 (32%), Positives = 38/73 (51%), Gaps = 2/73 (2%)
Frame = +3
Query: 409 GPPGVGKTSLASSIAAALDRKFVRISLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVAV 468
GPPG GKT LA ++A F+R+ V E + Y+G P R++ + R+A
Sbjct: 621 GPPGTGKTMLAKAVANHTTAAFIRV----VGSE-----FVQKYLGEGP-RMVRDVFRLAK 770
Query: 469 CN--PVMLLDEID 479
N ++ +DE+D
Sbjct: 771 ENAPAIIFIDEVD 809
>AV408284
Length = 425
Score = 32.0 bits (71), Expect = 0.44
Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 8/67 (11%)
Frame = +1
Query: 380 LAKVKQRIIEYLAVRKLKPD--ARGPVL------CFVGPPGVGKTSLASSIAAALDRKFV 431
L +K+ + E + + +PD + G +L GPPG GKT LA +IA F+
Sbjct: 1 LESIKEALFELVILPLKRPDLFSHGKLLGPQKGVLLYGPPGTGKTMLAKAIAKESGAVFI 180
Query: 432 RISLGGV 438
+ + +
Sbjct: 181 NVRISNL 201
>TC16655 homologue to UP|O99018 (O99018) Chloroplast protease precursor,
partial (39%)
Length = 934
Score = 31.6 bits (70), Expect = 0.58
Identities = 25/66 (37%), Positives = 33/66 (49%), Gaps = 7/66 (10%)
Frame = +3
Query: 376 DHYGLAKVKQRIIEYLAVRKLKPD------ARGPV-LCFVGPPGVGKTSLASSIAAALDR 428
D G+ + KQ +E + K KP+ AR P + +GPPG GKT LA +IA
Sbjct: 657 DVAGVDEAKQDFMEVVEFLK-KPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGEAGV 833
Query: 429 KFVRIS 434
F IS
Sbjct: 834 PFFSIS 851
>TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPase subunit
RPT4a, partial (49%)
Length = 648
Score = 30.8 bits (68), Expect = 0.99
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +2
Query: 409 GPPGVGKTSLASSIAAALDRKF 430
GPPG GKT LA +IA+ +D F
Sbjct: 581 GPPGTGKTLLARAIASNIDANF 646
>CB829689
Length = 558
Score = 30.4 bits (67), Expect = 1.3
Identities = 27/88 (30%), Positives = 43/88 (48%), Gaps = 12/88 (13%)
Frame = +1
Query: 403 PVLCFVGPPGVGKTSLASSIA----------AALDRKFVRISLGGVKDEADIRGHRRTYI 452
PVL +G PGVGKT+++ +A A ++R+ + + +G + I G + Y
Sbjct: 268 PVL--IGEPGVGKTAISEGLAQRIVQGDVPQALMNRRLISLDMGAL-----IAGAK--YR 420
Query: 453 GSMPGRLIDGLKRVAVCN--PVMLLDEI 478
G RL LK V + V+ +DEI
Sbjct: 421 GEFEDRLKAVLKEVTESDGQTVLFIDEI 504
>BP046346
Length = 476
Score = 29.6 bits (65), Expect = 2.2
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = -3
Query: 769 IHIHFPAGAIPKDGPSAGVTLVTALVSLFS 798
+H+HFPA ++GP + LV A+ +L++
Sbjct: 447 VHLHFPAHMSRRNGPKTYILLVCAMGALYA 358
>TC13343 similar to UP|Q9FMR9 (Q9FMR9) Ruv DNA-helicase-like protein,
partial (21%)
Length = 518
Score = 29.6 bits (65), Expect = 2.2
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = +1
Query: 402 GPVLCFVGPPGVGKTSLASSIAAALDRK 429
G L GPPG GKT+LA I L K
Sbjct: 358 GRALLLAGPPGTGKTALALGICQELGTK 441
>BP040113
Length = 439
Score = 29.6 bits (65), Expect = 2.2
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = +2
Query: 402 GPVLCFVGPPGVGKTSLASSIAAALDRK 429
G L GPPG GKT+LA I L K
Sbjct: 278 GRALLLAGPPGTGKTALALGICQELGTK 361
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.135 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,164,196
Number of Sequences: 28460
Number of extensions: 141170
Number of successful extensions: 743
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 737
length of query: 888
length of database: 4,897,600
effective HSP length: 98
effective length of query: 790
effective length of database: 2,108,520
effective search space: 1665730800
effective search space used: 1665730800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0550.4