BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0546.8
(898 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11884 125 3e-29
CB828260 102 3e-22
NP459593 Pge1 protein [Lotus japonicus] 80 1e-15
TC13387 63 2e-10
AV769506 59 4e-09
BP038017 55 5e-08
CN825057 43 2e-04
AV425218 40 0.002
TC16246 weakly similar to UP|Q8S2M2 (Q8S2M2) Far-red impaired re... 37 0.014
TC12114 weakly similar to GB|BAC16510.1|23237937|AP005198 transp... 33 0.20
AV776853 33 0.26
TC14104 GB|CAA61590.1|897773|LJAS2GENE asparagine synthase (glut... 32 0.59
BP066148 30 1.3
BP038715 30 1.3
TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-... 30 1.7
BP064113 30 2.2
TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription fact... 29 2.9
TC15586 weakly similar to UP|Q8LEJ2 (Q8LEJ2) Nematode resistance... 28 5.0
TC14295 similar to UP|O03990 (O03990) RAD23, isoform I, partial ... 28 5.0
BP041113 28 5.0
>TC11884
Length = 557
Score = 125 bits (314), Expect = 3e-29
Identities = 59/135 (43%), Positives = 83/135 (60%)
Frame = +1
Query: 396 HMGCTTTNRVEGAHSKLKKLLSDSKGDLVIAWTAMNKLIIMQHDKIKESFQLSIFVTEHS 455
H G TT+NR EGAH+ LKK+L D KGDL +W A + L +H +I SF+ SI +H
Sbjct: 1 HFGTTTSNRAEGAHASLKKMLRDCKGDLATSWDASHSLTCNRHTEILASFERSIHRIDHI 180
Query: 456 LNDPFYKHLRGFVSRAALHIIVKEKTQIGMIGVGGIYCECVLRRTHGLPCACELMKFESV 515
PFY ++RGFVS L ++ E ++ G C+C+LR TH LPC CEL +E +
Sbjct: 181 FMCPFYTNIRGFVSNKCLQLLDAEHIRMKSYG----RCDCLLRETHELPCGCELAGYERI 348
Query: 516 PLLAIHPHWRKLTWD 530
AI+P W++L+W+
Sbjct: 349 SYEAIYPFWKRLSWE 393
>CB828260
Length = 557
Score = 102 bits (254), Expect = 3e-22
Identities = 55/132 (41%), Positives = 78/132 (58%), Gaps = 2/132 (1%)
Frame = +3
Query: 48 GSDKRRAVMTLGCERGGKYKPSTSVLKRNRTGTKKCNCPFRLRAR--RSNKDKMWTVLVH 105
G+ R+A + LGCE+ GKY P T T+K CPFRL+ R ++ D+ W + V
Sbjct: 162 GNGSRKAYVMLGCEKHGKYVPYRDPDLVEGTRTQKTECPFRLKGRPMKNGTDRDWRLKVM 341
Query: 106 SGIHNHDTAEVLQGHSYVGRLNPEEKAMVGEMIEERVKASDILIAIRKHNPTNLTRIKQI 165
G HNH+ A L GH++VGRLN EEK V +M + V +L+ ++++NP NLT I QI
Sbjct: 342 EGAHNHEPARSLLGHNFVGRLNSEEKEQVEKMSKSWVLPRKMLLTLKENNPLNLTTISQI 521
Query: 166 YNEKQAYNRLKR 177
Y A RL++
Sbjct: 522 YG---ACKRLRK 548
>NP459593 Pge1 protein [Lotus japonicus]
Length = 633
Score = 80.5 bits (197), Expect = 1e-15
Identities = 35/58 (60%), Positives = 47/58 (80%)
Frame = +1
Query: 10 TKAFTTSQAFESSKDLVNWAKAVGKEHGYVIIIQKSDYGSDKRRAVMTLGCERGGKYK 67
T A ++AF S DL++WA+ VGKE+GYV+I+ +SDYGS KR+ ++TLGCERGGKYK
Sbjct: 442 TTATKIAEAFASHTDLIDWARCVGKENGYVVIVIRSDYGSAKRKPLITLGCERGGKYK 615
>TC13387
Length = 511
Score = 63.2 bits (152), Expect = 2e-10
Identities = 33/101 (32%), Positives = 54/101 (52%)
Frame = +1
Query: 779 EDKWMMIPNMAYLISTTYKFIVLTIANTGCNTFYPLKGKADLVEEHKFMPIVLVNKDHFV 838
EDKWM +P M YL++T ++ + ++I+ GC ++ PL+G+ H+ + I V +HFV
Sbjct: 4 EDKWMQLPEMGYLVATRFQVVFISISFQGCWSYLPLRGEGP-PPVHRVIAIGHV-INHFV 177
Query: 839 GVTLREGHPMPTTAHLWAYNCHPDARKWEEPYKERIEEYAA 879
+ L GH MP A W P + W PY + + +
Sbjct: 178 QLHLTLGHSMPPIALQWERYVDPISVSWCVPYVTHLHRFTS 300
>AV769506
Length = 345
Score = 58.5 bits (140), Expect = 4e-09
Identities = 26/53 (49%), Positives = 36/53 (67%), Gaps = 1/53 (1%)
Frame = +1
Query: 17 QAFESSKDLVNWAKAVGKEHGYVIIIQKSDY-GSDKRRAVMTLGCERGGKYKP 68
Q F + DL+NW + E+GYV +I KSDY G++ R+A + LGCE+ GKY P
Sbjct: 37 QIFPTRDDLINWVHGIAIENGYVTLITKSDYGGNESRKAYVMLGCEKHGKYVP 195
>BP038017
Length = 570
Score = 55.1 bits (131), Expect = 5e-08
Identities = 39/163 (23%), Positives = 70/163 (42%)
Frame = +2
Query: 151 IRKHNPTNLTRIKQIYNEKQAYNRLKRGSLTEMQHLMKLLEEHKYAHWSRVQDGTDVVRS 210
I+ N T T +K ++K+ L R + + + K+ ++ +++ D + + +
Sbjct: 101 IQXRNTTPFTYVKS--SQKRT---LGRDAQNLLNYFKKMQGKNPGFYYAIQLDDENRMIN 265
Query: 211 LFWAHPDSIHLFNEFPHVVILDSTYKTNRYRIPLLEMVGVTSTNLTYSIAFAYMQNEQKD 270
+FWA S +N F VI D+ Y+ N+Y++P GV A + +E +
Sbjct: 266 VFWADARSRSAYNYFGDAVIFDTMYRPNQYQVPFAPFTGVNHHGQNVLFGCALLLDESES 445
Query: 271 EVVWAVNRLKDLIINEDNLPKVFVTDKDQVLMEALETVFPAAR 313
W L D P TD+D+ + A+ VFP R
Sbjct: 446 SFTWLFRTW--LSAMNDRPPVSITTDQDRAIQAAVAQVFPETR 568
>CN825057
Length = 721
Score = 43.1 bits (100), Expect = 2e-04
Identities = 47/225 (20%), Positives = 86/225 (37%), Gaps = 8/225 (3%)
Frame = +1
Query: 34 KEHGYVIIIQKSDYGSDKRRAV-MTLGCERGGKYKPSTSVLKRNRTGTKKCNCPFRLRAR 92
K G+ I+ S + + C R G P + R+ KK +C + +
Sbjct: 28 KSMGFTTSIKNSRRSKKTKEFIDAKFACSRYG-VTPESDGGSNRRSSVKKTDCKACMHVK 204
Query: 93 RSNKDKMWTVLVHSGIHNHDTAEVLQGHSYVGR---LNPEEKAMVGEMIEERVKASDILI 149
R D W + HNH+ L H + R L + + + ER + + +
Sbjct: 205 RK-PDGKWIIHEFIKEHNHELLPALAYHFRIHRNVKLAEKNNMDILHAVSERTRKMYVEM 381
Query: 150 AIRKHNPTNLTRIKQIYNE---KQAYNRLKRGSLTEMQHLMKLLE-EHKYAHWSRVQDGT 205
+ + N+ + N+ K Y + G M K ++ E+ +S +
Sbjct: 382 SRQSGGCLNIESLVGDLNDQFKKGQYLAMDEGDAQVMLEYFKHIQKENPNFFYSIDLNEE 561
Query: 206 DVVRSLFWAHPDSIHLFNEFPHVVILDSTYKTNRYRIPLLEMVGV 250
+R++FW SI+ + F VV D++Y + ++P VGV
Sbjct: 562 QRLRNIFWIDAKSINDYLSFNDVVSFDTSYIKSNEKLPFAPFVGV 696
>AV425218
Length = 419
Score = 39.7 bits (91), Expect = 0.002
Identities = 25/121 (20%), Positives = 50/121 (40%), Gaps = 13/121 (10%)
Frame = +2
Query: 243 PLLEMVGVTSTNLTYSIAFAYMQNEQKDEVVWAVNRLKDLIINEDNLPKVFVTDKDQVLM 302
PL +VGV + A + +E + VW L + P+ +TD + +
Sbjct: 5 PLATLVGVNHHGQSVLFGCALLSSEDSESFVWLFQSLLHCMSGVP--PQGIITDHSEAMK 178
Query: 303 EALETVFPAARCLLCQFHVLK-------------SVTGEMKKLVKDIETREDITDRWNRL 349
+A+ETV P+ R C +++K S+ ++ +V D ++ W ++
Sbjct: 179 KAIETVLPSTRHRWCLSYIMKKLPQKLLGYAQYESIRHHLQNVVYDAVVIDEFERNWKKI 358
Query: 350 M 350
+
Sbjct: 359 V 361
>TC16246 weakly similar to UP|Q8S2M2 (Q8S2M2) Far-red impaired response
protein-like, partial (4%)
Length = 660
Score = 37.0 bits (84), Expect = 0.014
Identities = 18/47 (38%), Positives = 26/47 (55%)
Frame = +2
Query: 204 GTDVVRSLFWAHPDSIHLFNEFPHVVILDSTYKTNRYRIPLLEMVGV 250
G + + +LFW S +N F V+ DSTYK N+Y PL+ + V
Sbjct: 227 GDESLENLFWCDGVSRMDYNVFGDVIAFDSTYKKNKYNKPLVVFLHV 367
>TC12114 weakly similar to GB|BAC16510.1|23237937|AP005198 transposase-like
{Oryza sativa (japonica cultivar-group);}, partial (8%)
Length = 488
Score = 33.1 bits (74), Expect = 0.20
Identities = 22/87 (25%), Positives = 36/87 (41%)
Frame = +1
Query: 188 KLLEEHKYAHWSRVQDGTDVVRSLFWAHPDSIHLFNEFPHVVILDSTYKTNRYRIPLLEM 247
KL+E + H ++ D D + ++FW + + F +V LDSTY T+ PL
Sbjct: 76 KLVENSPFYHAYQLDD-EDQITNVFWVDARMLIDYGYFGDMVSLDSTYCTHSSNRPLAVF 252
Query: 248 VGVTSTNLTYSIAFAYMQNEQKDEVVW 274
G A + +E + W
Sbjct: 253 SGFNHHRKAVIFGAALLYDETTESY*W 333
>AV776853
Length = 625
Score = 32.7 bits (73), Expect = 0.26
Identities = 27/99 (27%), Positives = 40/99 (40%), Gaps = 5/99 (5%)
Frame = +1
Query: 19 FESSKDLVNWAKAVGKEHGYVIIIQKSDYGSDKRRAV--MTLGCERGG---KYKPSTSVL 73
F S ++ + +A K G+ I++K D G D V C R G K + +
Sbjct: 244 FGSEEEAYQFYQAYAKYQGF--IVRKDDIGRDYHGNVNMRQFVCNRQGLRSKKHYNRTDR 417
Query: 74 KRNRTGTKKCNCPFRLRARRSNKDKMWTVLVHSGIHNHD 112
KR+ NC +LR K W V+ HNH+
Sbjct: 418 KRDHKPVTHTNCLAKLRVHLDYKIGKWKVVSFEECHNHE 534
>TC14104 GB|CAA61590.1|897773|LJAS2GENE asparagine synthase
(glutamine-hydrolysing) {Lotus corniculatus var.
japonicus;} , complete
Length = 2259
Score = 31.6 bits (70), Expect = 0.59
Identities = 31/124 (25%), Positives = 55/124 (44%), Gaps = 1/124 (0%)
Frame = +3
Query: 165 IYNEKQAYNRLKRGSLTEMQHLMKLLEEHKYAHWSRVQDGTDVVRSLFWAHPDS-IHLFN 223
++NE Q+ G + + L K L HK+ R DV+ L+ H ++ + + +
Sbjct: 339 LFNEDQSIIVTVNGEIFNHEELRKQLPNHKF----RTGCDCDVIAHLYEEHGENFVDMLD 506
Query: 224 EFPHVVILDSTYKTNRYRIPLLEMVGVTSTNLTYSIAFAYMQNEQKDEVVWAVNRLKDLI 283
V+LD+ + N + + + +GVTS + Y + D VW + LK L
Sbjct: 507 GIFSFVLLDT--RDNSFLV-ARDAIGVTSLYIGYGL----------DGSVWIASELKGL- 644
Query: 284 INED 287
N+D
Sbjct: 645 -NDD 653
>BP066148
Length = 477
Score = 30.4 bits (67), Expect = 1.3
Identities = 17/57 (29%), Positives = 30/57 (51%)
Frame = +1
Query: 116 VLQGHSYVGRLNPEEKAMVGEMIEERVKASDILIAIRKHNPTNLTRIKQIYNEKQAY 172
V GH+ + NPE ++ + +L+ ++++NP+NLT I QIY + Y
Sbjct: 4 VTTGHNLLVV*NPERRSKWKK*QGVGFHPRKMLLTLKENNPSNLTTISQIYIQIYKY 174
>BP038715
Length = 530
Score = 30.4 bits (67), Expect = 1.3
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +2
Query: 561 GVRMSIIESLRMITYPETTSMCAPTKKKTRGRPMGSTNKPK 601
GV + + ++T P+T S K RGRP GS NKPK
Sbjct: 95 GVESASPTTPSVVTTPQTNSS---QKSAQRGRPKGSKNKPK 208
>TC13802 similar to UP|Q9M1T1 (Q9M1T1) Sugar-phosphate isomerase-like
protein, partial (41%)
Length = 522
Score = 30.0 bits (66), Expect = 1.7
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +2
Query: 633 SNTTPLQEPSPKVRSQKASKASKASKASKVSTASKASKVQSP 674
++TTP PSP S + +S + AS S+ +K+ K SP
Sbjct: 140 TSTTPKPSPSPAPSSTLRAPSSSPASASPASSPTKSPKPSSP 265
>BP064113
Length = 480
Score = 29.6 bits (65), Expect = 2.2
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -3
Query: 507 CELMKFESVPLLAIHPHWRKLTWD 530
CEL +E + I+P W++L+W+
Sbjct: 430 CELTGYERISYEXIYPFWKRLSWE 359
>TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription factor 1,
partial (41%)
Length = 1717
Score = 29.3 bits (64), Expect = 2.9
Identities = 16/54 (29%), Positives = 26/54 (47%)
Frame = +1
Query: 84 NCPFRLRARRSNKDKMWTVLVHSGIHNHDTAEVLQGHSYVGRLNPEEKAMVGEM 137
+CP + + +RS D+ V + G HNH ++ S GR ++VG M
Sbjct: 673 SCPVKKKVQRSVDDQSVLVATYEGEHNHPQPSQMEATSGSGR----NVSLVGSM 822
>TC15586 weakly similar to UP|Q8LEJ2 (Q8LEJ2) Nematode resistance
protein-like protein, partial (32%)
Length = 773
Score = 28.5 bits (62), Expect = 5.0
Identities = 16/49 (32%), Positives = 26/49 (52%)
Frame = +2
Query: 641 PSPKVRSQKASKASKASKASKVSTASKASKVQSPLIRMVCTKNKKPVSS 689
PSP+VRS+ + ++K + ++ A + + SP R V KP SS
Sbjct: 422 PSPRVRSRSSRSSAKTRRRIQIHAAPRQPLI-SPPARRVPEATVKPASS 565
>TC14295 similar to UP|O03990 (O03990) RAD23, isoform I, partial (29%)
Length = 622
Score = 28.5 bits (62), Expect = 5.0
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +2
Query: 595 GSTNKPKKFAKGCGETSTKRIPCRW 619
GSTNK + K CG ++T C W
Sbjct: 410 GSTNKCSSYFKPCGVSATSNSYCNW 484
>BP041113
Length = 503
Score = 28.5 bits (62), Expect = 5.0
Identities = 11/29 (37%), Positives = 19/29 (64%), Gaps = 1/29 (3%)
Frame = +1
Query: 779 EDKWMMIPNMAYLISTTYKFI-VLTIANT 806
EDKW+ +P Y +++ Y F+ T+A+T
Sbjct: 226 EDKWLWLPGGTYTVNSAYSFLQAPTLADT 312
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.134 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,063,598
Number of Sequences: 28460
Number of extensions: 227227
Number of successful extensions: 1200
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 1182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1193
length of query: 898
length of database: 4,897,600
effective HSP length: 98
effective length of query: 800
effective length of database: 2,108,520
effective search space: 1686816000
effective search space used: 1686816000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0546.8