BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.13
(545 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9544 UP|AAO92441 (AAO92441) Homoglutathione synthetase, complete 1089 0.0
TC17235 UP|Q93XE5 (Q93XE5) Glutathione synthetase, complete 753 0.0
AU251735 157 5e-39
TC13761 weakly similar to GB|AAO63290.1|28950733|BT005226 At5g57... 32 0.26
AV778312 30 1.00
BP075132 29 2.2
TC18503 similar to UP|Q9DTD4 (Q9DTD4) ORF1, partial (3%) 29 2.2
TC10530 29 2.2
TC8627 similar to UP|O49260 (O49260) G5bf protein, partial (36%) 28 2.9
TC13261 weakly similar to UP|Q8S1K4 (Q8S1K4) PDI-like protein, p... 28 2.9
TC19260 28 3.8
BP077029 28 3.8
TC10527 28 3.8
TC9735 28 3.8
TC12482 similar to UP|Q9TD47 (Q9TD47) NADH dehydrogenase subunit... 28 3.8
TC19130 similar to UP|Q7YSZ8 (Q7YSZ8) GE-rich protein precursor,... 27 6.5
TC10065 similar to UP|AAQ62444 (AAQ62444) At1g59840, partial (27%) 27 6.5
TC9979 similar to UP|HIR5_MOUSE (Q9QZ23) HIRA-interacting protei... 27 6.5
TC19191 similar to UP|BAC98299 (BAC98299) LEXYL2 protein (Fragme... 27 8.5
>TC9544 UP|AAO92441 (AAO92441) Homoglutathione synthetase, complete
Length = 1860
Score = 1089 bits (2816), Expect = 0.0
Identities = 543/545 (99%), Positives = 544/545 (99%)
Frame = +3
Query: 1 MGVGGCGSGCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSV 60
MGVGGCGSGCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSV
Sbjct: 12 MGVGGCGSGCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSV 191
Query: 61 SSSPIVEEVNDSSSFDYHQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLV 120
SSSPIVEEVNDSSSFDYHQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLV
Sbjct: 192 SSSPIVEEVNDSSSFDYHQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLV 371
Query: 121 HLPFSLLPPPLPESYWKQACELAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLDI 180
HLPFSLLPPPLPESYWKQACELAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLDI
Sbjct: 372 HLPFSLLPPPLPESYWKQACELAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLDI 551
Query: 181 HSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILSH 240
HSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILSH
Sbjct: 552 HSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILSH 731
Query: 241 YGKLLGLDSIRVPANSATNKFAETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAVL 300
YGKLLGLDSIRVPANSATNKFAETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAVL
Sbjct: 732 YGKLLGLDSIRVPANSATNKFAETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAVL 911
Query: 301 RDTYHIPTIRKTLAEVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLL 360
R+ YHIPTIRKTLAEVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLL
Sbjct: 912 REKYHIPTIRKTLAEVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLL 1091
Query: 361 MEQSSAIKCPPISYHLVGTKKIQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDI 420
MEQSSAIKCPPISYHLVGTKKIQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDI
Sbjct: 1092MEQSSAIKCPPISYHLVGTKKIQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDI 1271
Query: 421 VKQAIEKPELFVMKPQREGGGNNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTAA 480
VKQAIEKPELFVMKPQREGGGNNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTAA
Sbjct: 1272VKQAIEKPELFVMKPQREGGGNNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTAA 1451
Query: 481 ILMRNGCWHKDHVLTEFGIFGTYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVID 540
ILMRNGCWHKDHVLTEFGIFGTYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVID
Sbjct: 1452ILMRNGCWHKDHVLTEFGIFGTYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVID 1631
Query: 541 SVYLT 545
SVYLT
Sbjct: 1632SVYLT 1646
>TC17235 UP|Q93XE5 (Q93XE5) Glutathione synthetase, complete
Length = 2152
Score = 753 bits (1945), Expect = 0.0
Identities = 379/536 (70%), Positives = 445/536 (82%), Gaps = 4/536 (0%)
Frame = +1
Query: 14 TSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLI----MSQHLSVSSSPIVEEV 69
T+ T S+ + +FS+ SF + LKLI QHL+++ P+
Sbjct: 55 TTSTKDSYRSFSTHTFSTAFRRQPPPPPPSSFRRKPLKLITMAQQQQHLTLA--PVNGNG 228
Query: 70 NDSSSFDYHQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLVHLPFSLLPP 129
+ ++ D +LL+ + DALV+A+L+GL++GDKSVQRSG VPGVGLVH PF+L P
Sbjct: 229 SITAPATTLLFDQQLLQKIADDALVWASLHGLVMGDKSVQRSGTVPGVGLVHAPFALFPT 408
Query: 130 PLPESYWKQACELAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLDIHSKMLEINK 189
PES W+QAC+LAP+FNELVDRVSLDGKFLQESLSRTK+ DEFTSRLLDIHSKML INK
Sbjct: 409 LFPESQWRQACDLAPIFNELVDRVSLDGKFLQESLSRTKEVDEFTSRLLDIHSKMLAINK 588
Query: 190 KEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILSHYGKLLGLDS 249
KEEIR+GLHRSDYMLDEKTK LLQIE+NTIS+SF+G+G + TELHR ILS YGKLLGLDS
Sbjct: 589 KEEIRLGLHRSDYMLDEKTKSLLQIELNTISSSFAGLGSLVTELHRYILSRYGKLLGLDS 768
Query: 250 IRVPANSATNKFAETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAVLRDTYHIPTI 309
+VPAN+A +FAE LA+AW EYN+PRAVIMI+VQ EE NMYDQH +SAVLRD + I +
Sbjct: 769 GKVPANNAATQFAEGLARAWTEYNDPRAVIMIVVQAEERNMYDQHFLSAVLRDRHQIAIV 948
Query: 310 RKTLAEVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLLMEQSSAIKC 369
RKTLAEVD+EG +LPDGTLSVDGQ IA+VYFRAGYTP DYPSESEWRARLLMEQSSAIKC
Sbjct: 949 RKTLAEVDREGEILPDGTLSVDGQRIAVVYFRAGYTPVDYPSESEWRARLLMEQSSAIKC 1128
Query: 370 PPISYHLVGTKKIQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDIVKQAIEKPE 429
P ISYHLVGTKKIQQELA P VLERF ENK D+AKLRECFAGLWSLDD DI ++AIEKPE
Sbjct: 1129PSISYHLVGTKKIQQELANPGVLERFLENKGDIAKLRECFAGLWSLDDYDITRKAIEKPE 1308
Query: 430 LFVMKPQREGGGNNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTAAILMRNGCWH 489
LFVMKPQREGGGNNIYG+ +RETL+KLQ++GS+EDAAYILMQRIFPT +AA+LMRNGCWH
Sbjct: 1309LFVMKPQREGGGNNIYGNAVRETLLKLQQAGSQEDAAYILMQRIFPTLSAAVLMRNGCWH 1488
Query: 490 KDHVLTEFGIFGTYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVIDSVYLT 545
KDH ++E GIF TYLRNKD+V++N++SGY++RTK+SSSDEGGV GF V+DSVYLT
Sbjct: 1489KDHAVSELGIFSTYLRNKDRVVMNNQSGYLMRTKISSSDEGGVAAGFAVLDSVYLT 1656
>AU251735
Length = 344
Score = 157 bits (396), Expect = 5e-39
Identities = 79/79 (100%), Positives = 79/79 (100%)
Frame = -2
Query: 31 SIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYHQIDPELLENVVY 90
SIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYHQIDPELLENVVY
Sbjct: 343 SIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYHQIDPELLENVVY 164
Query: 91 DALVYATLNGLLVGDKSVQ 109
DALVYATLNGLLVGDKSVQ
Sbjct: 163 DALVYATLNGLLVGDKSVQ 107
>TC13761 weakly similar to GB|AAO63290.1|28950733|BT005226 At5g57910
{Arabidopsis thaliana;}, partial (21%)
Length = 652
Score = 32.0 bits (71), Expect = 0.26
Identities = 20/48 (41%), Positives = 25/48 (51%)
Frame = +2
Query: 19 HSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIV 66
HSF+H+ F SF S SL+ S P H L L+ S LS S +V
Sbjct: 131 HSFTHTLFLSF--------SFSLTFSLPPHLLLLLSSSSLSAFSPFVV 250
>AV778312
Length = 610
Score = 30.0 bits (66), Expect = 1.00
Identities = 17/34 (50%), Positives = 18/34 (52%)
Frame = +1
Query: 20 SFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLI 53
SFSHS PS SSI H QSL+ HH I
Sbjct: 118 SFSHS--PSSSSISFHFQSLNFISILHNHHASFI 213
>BP075132
Length = 269
Score = 28.9 bits (63), Expect = 2.2
Identities = 16/49 (32%), Positives = 21/49 (42%)
Frame = -1
Query: 6 CGSGCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIM 54
C C I S +F H P S LHH S S + SF +L++
Sbjct: 254 CFESCSCIKITFSFAFDHKILPPGSERALHHVSASSASSFSYKTQRLLL 108
>TC18503 similar to UP|Q9DTD4 (Q9DTD4) ORF1, partial (3%)
Length = 504
Score = 28.9 bits (63), Expect = 2.2
Identities = 20/45 (44%), Positives = 22/45 (48%), Gaps = 2/45 (4%)
Frame = +1
Query: 15 SGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHL--KLIMSQH 57
S TS SFS S S Q HQ S S P+HH L+ SQH
Sbjct: 4 SNTSDSFSFSAATVVSPSQPRHQPPSPSPPPPQHHRIHHLLQSQH 138
>TC10530
Length = 533
Score = 28.9 bits (63), Expect = 2.2
Identities = 16/32 (50%), Positives = 18/32 (56%)
Frame = +2
Query: 18 SHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHH 49
SHSF H F+SIQL SLSL PK +
Sbjct: 80 SHSFLHQPLVIFASIQLQSLSLSLWSFGPKSY 175
>TC8627 similar to UP|O49260 (O49260) G5bf protein, partial (36%)
Length = 515
Score = 28.5 bits (62), Expect = 2.9
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +1
Query: 24 STFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSS 73
+T FSSIQ H S+ + +L++ Q +S SP+ ++D S
Sbjct: 7 NTLQIFSSIQHQHSLWSVQSYLTRSMARLVVPQQNQLSFSPLASSLSDFS 156
>TC13261 weakly similar to UP|Q8S1K4 (Q8S1K4) PDI-like protein, partial
(17%)
Length = 692
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/58 (31%), Positives = 28/58 (48%)
Frame = -1
Query: 21 FSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYH 78
F+ ++ PSF SI + +S+S + PKH K + EE +D S+ YH
Sbjct: 182 FTRASVPSFISILITLSLISVSQAPPKHKKK-----------KRVEEEESDIISYQYH 42
>TC19260
Length = 498
Score = 28.1 bits (61), Expect = 3.8
Identities = 21/72 (29%), Positives = 31/72 (42%)
Frame = +3
Query: 67 EEVNDSSSFDYHQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLVHLPFSL 126
++++ S DP++L + D YA +G L K V +S V GVG +
Sbjct: 15 KDLSQCPSISAEVSDPDVLTPSLGDGPSYAGCSGNLFKRKVVDQSHPVSGVG----ELKM 182
Query: 127 LPPPLPESYWKQ 138
P L E W Q
Sbjct: 183 KPVKLEEE*WMQ 218
>BP077029
Length = 486
Score = 28.1 bits (61), Expect = 3.8
Identities = 19/58 (32%), Positives = 28/58 (47%), Gaps = 10/58 (17%)
Frame = +1
Query: 19 HSFSHSTFPSFSSIQLHHQSLSLSLSF--------PKHHLK--LIMSQHLSVSSSPIV 66
H +H+ FP S Q+ HQ+L++ L + P H L+ S HLSV P +
Sbjct: 205 HPITHN-FPHLSFCQIFHQALTIQLEYYLGFSWPLPSHPSSEALVPSTHLSVL*EPFL 375
>TC10527
Length = 529
Score = 28.1 bits (61), Expect = 3.8
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = -1
Query: 19 HSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQ 56
HS +H +F + S L HQ S S KHH ++ +Q
Sbjct: 343 HSLNHPSFVTMSPTHLIHQGGSNQNSCSKHHNVVLKTQ 230
>TC9735
Length = 523
Score = 28.1 bits (61), Expect = 3.8
Identities = 19/63 (30%), Positives = 25/63 (39%)
Frame = +1
Query: 13 ITSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDS 72
+ S SH+F+H FS Q H S P H L S S S+P E + +
Sbjct: 154 LPSALSHTFTHPKVSIFSQSQTTHYPQFSSKFKPPHFLAYCSSGSSSNGSNPSDEVILQN 333
Query: 73 SSF 75
F
Sbjct: 334 DIF 342
>TC12482 similar to UP|Q9TD47 (Q9TD47) NADH dehydrogenase subunit II,
partial (5%)
Length = 720
Score = 28.1 bits (61), Expect = 3.8
Identities = 20/59 (33%), Positives = 30/59 (49%)
Frame = +2
Query: 148 ELVDRVSLDGKFLQESLSRTKKADEFTSRLLDIHSKMLEINKKEEIRMGLHRSDYMLDE 206
E+ D+V K ++E S K F L + +KMLEI E + G+ +DY LD+
Sbjct: 218 EIADKVCEIVKKIREDSSNVTKETNFDDCLDE--TKMLEIYDAIEEQFGISFNDYHLDK 388
>TC19130 similar to UP|Q7YSZ8 (Q7YSZ8) GE-rich protein precursor, partial
(20%)
Length = 603
Score = 27.3 bits (59), Expect = 6.5
Identities = 18/64 (28%), Positives = 32/64 (49%)
Frame = +3
Query: 17 TSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFD 76
T S HST SFSS++L ++S SF H++ M+ +++ + S S +
Sbjct: 66 TFSSLHHSTSYSFSSLKLSISTMSFFFSF---HVQTTMASPKLATTASSHGDAATSFSRE 236
Query: 77 YHQI 80
+H +
Sbjct: 237 HHHV 248
>TC10065 similar to UP|AAQ62444 (AAQ62444) At1g59840, partial (27%)
Length = 439
Score = 27.3 bits (59), Expect = 6.5
Identities = 21/51 (41%), Positives = 22/51 (42%), Gaps = 7/51 (13%)
Frame = +2
Query: 2 GVGGCGS--GCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSL-----SLSF 45
G G C GCC SHS SH F IQ H +SL SLSF
Sbjct: 50 GSGNCVPCRGCCHCQHSHSHSHSH-----FQEIQNHRIPISLLHLFISLSF 187
>TC9979 similar to UP|HIR5_MOUSE (Q9QZ23) HIRA-interacting protein 5
(mHIRIP5), partial (36%)
Length = 1304
Score = 27.3 bits (59), Expect = 6.5
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 416 DDSDIVKQAIEKPELFVMKPQREGGGNNIY-GDDLRETLIKLQKSGS 461
DDS+IV E E + ++ GG+ +Y G D ++KLQ G+
Sbjct: 524 DDSEIVAMIKELLETRIRPAVQDDGGDIVYRGSDPDTGIVKLQMQGA 664
>TC19191 similar to UP|BAC98299 (BAC98299) LEXYL2 protein (Fragment),
partial (31%)
Length = 770
Score = 26.9 bits (58), Expect = 8.5
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = -1
Query: 285 PEEHNMYDQHMISAVLRDTYHIPTI 309
P HN + QH ++ +LR+ H P +
Sbjct: 344 PSFHNAHQQHQVTCILRNGTHDPQL 270
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,642,675
Number of Sequences: 28460
Number of extensions: 138605
Number of successful extensions: 1073
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 1056
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1071
length of query: 545
length of database: 4,897,600
effective HSP length: 95
effective length of query: 450
effective length of database: 2,193,900
effective search space: 987255000
effective search space used: 987255000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0544.13