BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0520.5
(262 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18710 94 3e-20
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 57 4e-09
BP085058 42 1e-04
AV780705 41 2e-04
TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine liga... 33 0.050
TC14560 weakly similar to UP|Q8H6C1 (Q8H6C1) Oligomycin sensitiv... 29 0.94
AV421890 28 1.6
TC18223 27 2.7
TC15109 similar to UP|Q9FVE9 (Q9FVE9) Cytosolic aconitase, parti... 27 4.7
TC17798 similar to GB|AAM19907.1|20453337|AY097391 At2g42750/F7D... 26 6.1
BP060544 26 8.0
TC16443 26 8.0
>TC18710
Length = 843
Score = 93.6 bits (231), Expect = 3e-20
Identities = 52/116 (44%), Positives = 66/116 (56%), Gaps = 1/116 (0%)
Frame = +2
Query: 2 RDNIILAQEVMHTIHTQKK-GRGLVAIKIDLEKAYDRVSWDFLRATLYDFGFPPRIVDLI 60
+DN+++ QE H I+ GR IK+D+ KAYDRV W FL ++L FGF V +I
Sbjct: 488 QDNLLIVQEAFHAINRPGALGRNHSIIKLDMNKAYDRVEWKFLESSLLAFGFSTNWVKMI 667
Query: 61 MWGVKCPKLSILWNCSKLPSFAPQRGLRQGDPLSPYLFVLCMERLAIQIQNLVTQG 116
M V + N P PQRGLRQGDP SPYLF+ ME L++ IQN G
Sbjct: 668 MILVSGVSYNYKINGVVGPKLLPQRGLRQGDPFSPYLFLFTMEVLSLLIQNSFNMG 835
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 56.6 bits (135), Expect = 4e-09
Identities = 36/122 (29%), Positives = 60/122 (48%)
Frame = -3
Query: 130 ISHLFFADDVLLFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQINL 189
ISHL FAD++++F E + ++NN L F SG+ N KS + + QI
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSEVFILVKETTKNQI-- 200
Query: 190 SHIAGISRASDLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVCLAKS 249
+++ G + LG+P L ++ + L+ + S+L W L+ AGR+ L S
Sbjct: 199 TNMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLINS 20
Query: 250 VL 251
+L
Sbjct: 19 IL 14
>BP085058
Length = 452
Score = 42.0 bits (97), Expect = 1e-04
Identities = 23/60 (38%), Positives = 33/60 (54%)
Frame = +2
Query: 192 IAGISRASDLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVCLAKSVL 251
I I ++GKY+ P+ +G V K F + ++ SRL K LLN+ R+ LAK VL
Sbjct: 101 ITSIPFTDNIGKYMSFPIHQGHV*KEDFVFTLDRVISRLAC*KAYLLNKPARLTLAKPVL 280
>AV780705
Length = 524
Score = 41.2 bits (95), Expect = 2e-04
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = -2
Query: 200 DLGKYLGIPLLKGRVTKAHFASLIGKMSSRLTSWKNNLLNRAGRVCLAKSVL 251
D G YLG+P + GR + K+ +L WK LLN+AG+ L K+++
Sbjct: 496 DPGHYLGLPAIWGRNKSHSLVWIEEKVKEKLEGWKETLLNQAGKEVLIKAII 341
>TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine ligase {Lotus
corniculatus var. japonicus;} , complete
Length = 1310
Score = 33.1 bits (74), Expect = 0.050
Identities = 16/54 (29%), Positives = 26/54 (47%)
Frame = +1
Query: 133 LFFADDVLLFCQASKEQLQLVNNTLKEFCEASGMKVNLDKSRMLFSKAVDHRRQ 186
L F D ++FC+A+ + + L + A G+ +NLDKS + RQ
Sbjct: 1102 LLFVGDGIIFCRATTPEENKIREILLMYQSAPGLLINLDKSELSLG*CTPRDRQ 1263
>TC14560 weakly similar to UP|Q8H6C1 (Q8H6C1) Oligomycin sensitivity
conferring protein, partial (83%)
Length = 1061
Score = 28.9 bits (63), Expect = 0.94
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = +1
Query: 52 FPPRIVDLIMWGVKCPKLSILWNCSKLPSFAP 83
+PP I+ + G + PKL LW C ++ P
Sbjct: 193 WPPEIIPPMCQGKRIPKLRFLWQCLEVQETMP 288
>AV421890
Length = 366
Score = 28.1 bits (61), Expect = 1.6
Identities = 11/32 (34%), Positives = 16/32 (49%)
Frame = +2
Query: 53 PPRIVDLIMWGVKCPKLSILWNCSKLPSFAPQ 84
PP++ I+W +L + W SK P PQ
Sbjct: 65 PPKVQPNILWKNSW*RLPLTWETSKFPQVLPQ 160
>TC18223
Length = 601
Score = 27.3 bits (59), Expect = 2.7
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = +2
Query: 29 IDLEKAYDRVSWDFLR 44
+DL+ +YD++SW FLR
Sbjct: 320 VDLQGSYDKISWIFLR 367
>TC15109 similar to UP|Q9FVE9 (Q9FVE9) Cytosolic aconitase, partial (46%)
Length = 1237
Score = 26.6 bits (57), Expect = 4.7
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = -1
Query: 64 VKCPKLSILWNCSKLPSFAPQRGLRQGDPLSPYLFVLCMERLA 106
+ CP L SK +F+P+ R P SP+ +L M LA
Sbjct: 1060 ISCPSLLYFNAASKTDNFSPEGIWRVLGPTSPFKNLLTMRILA 932
>TC17798 similar to GB|AAM19907.1|20453337|AY097391 At2g42750/F7D19.25
{Arabidopsis thaliana;}, partial (29%)
Length = 548
Score = 26.2 bits (56), Expect = 6.1
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -2
Query: 43 LRATLYDFGFPPRIVDLIMWGVKCPKLSILWNCSKLP 79
L +Y++G + +LI W C ++ + C +LP
Sbjct: 409 LEFCIYNYGM*QTMDELITWDQMCREIGVTCPCLRLP 299
>BP060544
Length = 446
Score = 25.8 bits (55), Expect = 8.0
Identities = 13/48 (27%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Frame = +3
Query: 147 KEQLQLVNNT-LKEFCEASGMKVNLDKSRMLFSKAVDHRRQINLSHIA 193
K+++++ N L FC M + + + FSK Q ++SH+A
Sbjct: 66 KQKVKITGNMFLNFFCSGPFMCIRISMKNIGFSKTAKCNSQSSISHVA 209
>TC16443
Length = 987
Score = 25.8 bits (55), Expect = 8.0
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +1
Query: 64 VKCPKLSILWNCSKLPSFAPQRGLRQGDPLSPYLFVLCMERL 105
+ CP L + NC ++ + R G L PYLF+ + R+
Sbjct: 850 ISCPLLCKM-NCRRMKMICRRIP*RNGGQLQPYLFLSPLHRM 972
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.327 0.141 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,614,157
Number of Sequences: 28460
Number of extensions: 63928
Number of successful extensions: 375
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 373
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 373
length of query: 262
length of database: 4,897,600
effective HSP length: 88
effective length of query: 174
effective length of database: 2,393,120
effective search space: 416402880
effective search space used: 416402880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0520.5