BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0490.17
(392 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU088785 140 5e-34
AU088950 134 2e-32
BP032748 99 9e-22
AV781067 77 6e-15
AV775483 73 9e-14
BP042395 62 2e-10
BP030183 57 7e-09
AV422277 55 2e-08
TC9821 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like prot... 54 6e-08
AV770305 48 3e-06
TC12747 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-l... 44 5e-05
TC11359 42 2e-04
AV780084 41 4e-04
TC18673 similar to UP|Q9LWY0 (Q9LWY0) ESTs AU029388(E30287), par... 39 0.001
AV776486 33 0.082
TC10205 weakly similar to UP|Q8RL77 (Q8RL77) HpaG, partial (10%) 32 0.24
BP028651 28 2.0
AV408303 28 2.6
TC11950 similar to UP|Q43573 (Q43573) Zn finger protein (Fragmen... 28 3.4
BI418523 28 3.4
>AU088785
Length = 773
Score = 140 bits (352), Expect = 5e-34
Identities = 74/163 (45%), Positives = 101/163 (61%)
Frame = +2
Query: 157 VRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIAT 216
V+++ S + K FH+ + CTN K+IAP S NTDGIHI+ S +V I + I T
Sbjct: 53 VQNIKSLNPKGFHMFVTKCTNVRLRKLKLIAP--ETSPNTDGIHISSSINVIIARNTIQT 226
Query: 217 GDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRI 276
GDDC+S+ G++++ I+ +KCGPGHG SIGSLG V+ I + N L T NG+RI
Sbjct: 227 GDDCISMIXGSENVFINRLKCGPGHGISIGSLGKYAEXREVKGIXIXNSALIGTTNGLRI 406
Query: 277 KTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSK 319
K+WP G + ++F +ITM NV NP+IIDQ Y S C K
Sbjct: 407 KSWPDKYG-GAASXISFSNITMENVKNPIIIDQXYQCSPNCQK 532
>AU088950
Length = 423
Score = 134 bits (338), Expect = 2e-32
Identities = 70/124 (56%), Positives = 83/124 (66%), Gaps = 2/124 (1%)
Frame = +2
Query: 3 MSLIRSTAIVLFLLLLS--GSAQSIVFDIRTFGGAPNSDITQAFLNAWKQACASTSPSKI 60
M L S I++F+ L SAQS V DI FG PNSDITQAF +AW +ACAS +PSKI
Sbjct: 38 MGLKLSIIILIFVSFLFDFSSAQSGVIDISKFGVKPNSDITQAFTSAWNEACASPTPSKI 217
Query: 61 VISKATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNG 120
VI TY M A+ +KGPCKAPIE+QVDGTIQ+ +P+ L GA QWVT+ YV G
Sbjct: 218 VIPSXTYXMGALELKGPCKAPIEVQVDGTIQSSGNPSDLXGAXQWVTVSYVPXLPYPGXS 397
Query: 121 TFDG 124
FDG
Sbjct: 398 VFDG 409
>BP032748
Length = 453
Score = 99.4 bits (246), Expect = 9e-22
Identities = 49/111 (44%), Positives = 71/111 (63%)
Frame = -2
Query: 272 NGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVT 331
NG+RIK+WP G + + ++F +ITM NV NP+IIDQ+Y S C K+ PS ++I ++
Sbjct: 452 NGLRIKSWPDKYGGA-ASEISFSNITMENVKNPIIIDQEYQCSPNCQKK-PSLVRIRDIH 279
Query: 332 FKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGAPAMAKCANVKPI 382
F N+KGT+ + V CS PC G+ L DI L AP A+C+N+KP+
Sbjct: 278 FANVKGTTTSPIAVDFRCSKLYPCMGITLRDIDLKLGTAPTTARCSNIKPV 126
>AV781067
Length = 538
Score = 76.6 bits (187), Expect = 6e-15
Identities = 43/97 (44%), Positives = 59/97 (60%), Gaps = 1/97 (1%)
Frame = -2
Query: 270 TQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISN 329
+ NG+RIKTW GT VTNL FE+I M NV N + IDQ YC + C Q+ S + +++
Sbjct: 531 SDNGLRIKTWQG--GTGSVTNLRFENIQMENVRNCINIDQYYCLTKECRNQT-SAVHVND 361
Query: 330 VTFKNIKGTSETKDGVI-LNCSSGVPCEGVELSDISL 365
V++ NIKGT + + I CS V C + LSD+ L
Sbjct: 360 VSYINIKGTYDVRTPPIHFACSDSVACTNITLSDVEL 250
>AV775483
Length = 503
Score = 72.8 bits (177), Expect = 9e-14
Identities = 46/137 (33%), Positives = 69/137 (49%), Gaps = 2/137 (1%)
Frame = -2
Query: 255 EPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPS 314
E VE + V +C+ T TQN RIKT + G+ +TFE IT+ +P+IID Y P
Sbjct: 490 ETVEEVYVYDCSFTGTQNPARIKT--LSGGSGYARKITFEKITLTKAGDPIIIDPYYAP- 320
Query: 315 NLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISL--TFNGAPA 372
+ S + +S+VTF+ I+GTS + L+CS C + L I++ + +G
Sbjct: 319 -----EDDSSVPVSDVTFRGIRGTSSDDIAINLHCSP-PGCFYLVLDQINIVSSVSGKKT 158
Query: 373 MAKCANVKPIIIGKAPS 389
A C N + PS
Sbjct: 157 SASCQNAHGRVTSSVPS 107
>BP042395
Length = 480
Score = 61.6 bits (148), Expect = 2e-10
Identities = 27/65 (41%), Positives = 40/65 (61%)
Frame = -1
Query: 312 CPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGAP 371
CP+N + S +KIS+VT+K+I GTS T + +CSS PC G++L ++ LT+
Sbjct: 480 CPNNKGCPRQASGVKISDVTYKDIHGTSATPVAIKFDCSSKYPCNGIKLQEVKLTYKSQD 301
Query: 372 AMAKC 376
A A C
Sbjct: 300 AQASC 286
>BP030183
Length = 483
Score = 56.6 bits (135), Expect = 7e-09
Identities = 24/74 (32%), Positives = 47/74 (63%), Gaps = 4/74 (5%)
Frame = +3
Query: 26 VFDIRTFGGAPNS--DITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKA--P 81
++++ +FG P+ D TQAF+ AW+ C S +P +I++ + ++++ +GPC + P
Sbjct: 222 IYNVMSFGAKPDGVFDSTQAFMTAWQTVCHSPAPGRILVPAGRFLVSSMFFQGPCLSPHP 401
Query: 82 IEIQVDGTIQAPTD 95
+ +QV+GT+ A D
Sbjct: 402 VTLQVEGTVLASAD 443
>AV422277
Length = 454
Score = 55.1 bits (131), Expect = 2e-08
Identities = 37/118 (31%), Positives = 62/118 (52%)
Frame = +2
Query: 109 GYVDYFTLSGNGTFDGRGASAWKLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFF 168
G D NGT DG+G W + + ++ ++ + F ++ + +V KDS F+
Sbjct: 110 GVRDVVITGENGTIDGQGDVWWNMWRQRTLQFTRPNL-VEFVNSRNIIISNVIFKDSPFW 286
Query: 169 HVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG 226
++ + C+N I+AP DS NTDG+ S++V I +S I+TGDD V++ G
Sbjct: 287 NIHPVYCSNVVVRYVTILAPRDSP--NTDGVDPDSSSNVCIEDSYISTGDDLVAVKSG 454
>TC9821 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (61%)
Length = 1080
Score = 53.5 bits (127), Expect = 6e-08
Identities = 60/246 (24%), Positives = 110/246 (44%), Gaps = 14/246 (5%)
Frame = +3
Query: 157 VRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIAT 216
+ ++T +S +++ + C+N G I+AP S NTDGI+ T+ +I + I +
Sbjct: 105 ISNLTLVNSPSWNIHPVYCSNVIVQGITILAP--VTSPNTDGINPDSCTNTRIEDCYIVS 278
Query: 217 GDDCVSLGDG-----------NKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNC 265
GDDCV++ G KH+ I + C ++ +LG+ +E++ ++
Sbjct: 279 GDDCVAVKSGWDEYGISYGMPTKHLVIRRLTC-ISPTSAVIALGSE-MSGGIEDVRAEDI 452
Query: 266 TLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKI 325
N+++GVRIKT G V ++ +TM + + Y S+ + P+ I
Sbjct: 453 LAINSESGVRIKTAVGRGGY--VRDIYVRRMTMKTMKWVFWMTGDY-GSHADNNYDPNAI 623
Query: 326 K-ISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTF--NGAPAMAKCANVKPI 382
I N+ ++++ + T L SG P G+ +S ++L C +V I
Sbjct: 624 PVIENINYRDMVAENVT-TAPRLEGISGAPFTGICISHVTLELAQKAKKVPWTCTDVSGI 800
Query: 383 IIGKAP 388
G P
Sbjct: 801 SSGVTP 818
>AV770305
Length = 442
Score = 47.8 bits (112), Expect = 3e-06
Identities = 24/67 (35%), Positives = 37/67 (54%), Gaps = 1/67 (1%)
Frame = +2
Query: 76 GPCKAP-IEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQK 134
GPCK+ I IQ+ G I AP+ +W++I V++ T++G G DG G+S W+ +
Sbjct: 182 GPCKSSSINIQLQGNIVAPSFDAWGADKSKWISIHSVNHLTMNGGGKIDGLGSSGWERCR 361
Query: 135 ACGATCS 141
C S
Sbjct: 362 TCARPTS 382
>TC12747 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-like
protein, partial (43%)
Length = 624
Score = 43.9 bits (102), Expect = 5e-05
Identities = 44/173 (25%), Positives = 74/173 (42%), Gaps = 13/173 (7%)
Frame = +2
Query: 119 NGTFDGRGASAWKLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNF 178
+GT DG+G+ W L + G R I + T+ ++T +S + + + C
Sbjct: 107 DGTIDGQGSVWWDL-ISTGDLNYSRPHIIELIGSDNITISNLTILNSPSWGIHPVYCRYV 283
Query: 179 TFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG------------ 226
+ AP + S +T GI S + I SNI+TG D ++L G
Sbjct: 284 QIHNITVHAPPE--SPHTSGIVPDSSEYICIDKSNISTGHDAIALKSGWDEYGVAYGKPT 457
Query: 227 -NKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKT 278
N HI+ ++ G G + GS + NII + + N++ G+ +KT
Sbjct: 458 LNVHISSVYLQSSSGAGLAFGS----EMSGGISNIIAEQVHIINSKIGIELKT 604
>TC11359
Length = 544
Score = 42.0 bits (97), Expect = 2e-04
Identities = 21/57 (36%), Positives = 36/57 (62%), Gaps = 2/57 (3%)
Frame = -3
Query: 325 IKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTF--NGAPAMAKCANV 379
+K+++VTF+NI GT + V+L+C+ + C+ ++L I++T PA A C NV
Sbjct: 233 LKVTDVTFRNIHGTCTNEHAVVLDCAK-IGCDNIKLQQINITSIDLDKPASAICHNV 66
>AV780084
Length = 527
Score = 40.8 bits (94), Expect = 4e-04
Identities = 17/43 (39%), Positives = 29/43 (66%)
Frame = -3
Query: 323 SKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISL 365
S +++ N++F +I+GTS T++ + CS PCEG+ L +I L
Sbjct: 513 SAVQVRNISFTDIQGTSATEEAIKFACSDASPCEGLYL*NIFL 385
>TC18673 similar to UP|Q9LWY0 (Q9LWY0) ESTs AU029388(E30287), partial (23%)
Length = 560
Score = 38.9 bits (89), Expect = 0.001
Identities = 49/188 (26%), Positives = 78/188 (41%), Gaps = 12/188 (6%)
Frame = +2
Query: 190 DSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDG-----------NKHITIHN-VKC 237
+S NTDGI S DV I + I+TGDD +++ G + +I I V
Sbjct: 2 NSARANTDGIDPDSSDDVCIEDCYISTGDDLIAIKSGWDEYGIAYGRPSTNIVIRRLVGK 181
Query: 238 GPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDIT 297
G +IGS V + ++ ++ N +RIKT G V N+ ++T
Sbjct: 182 TQTSGIAIGS----EMSGGVSEVHAEDIHFYDSYNAIRIKTSRGRGGY--VRNIYISNMT 343
Query: 298 MVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEG 357
+VN+ + + Y + + I +T K++ G K IL G
Sbjct: 344 LVNIEYAITFNGLYGEHPDDAYDPNALPVIEKITIKDVIG-ENIKQAGILEGIEGDNFVN 520
Query: 358 VELSDISL 365
+ LS+ISL
Sbjct: 521 ICLSNISL 544
>AV776486
Length = 409
Score = 33.1 bits (74), Expect = 0.082
Identities = 17/33 (51%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +1
Query: 26 VFDIRTFG--GAPNSDITQAFLNAWKQACASTS 56
VFD+ +FG G ++D T AF AWK ACA S
Sbjct: 310 VFDVMSFGAVGDGSADDTAAFREAWKAACAVES 408
>TC10205 weakly similar to UP|Q8RL77 (Q8RL77) HpaG, partial (10%)
Length = 586
Score = 31.6 bits (70), Expect = 0.24
Identities = 18/51 (35%), Positives = 25/51 (48%)
Frame = -1
Query: 117 SGNGTFDGRGASAWKLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKF 167
+ N +D R K+ GATCS RS+ I + +S + S S SKF
Sbjct: 331 AANPNYDERNLERKSKLKSVGATCSCRSLRICWTGSHNSAISSSHSTSSKF 179
>BP028651
Length = 277
Score = 28.5 bits (62), Expect = 2.0
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2
Query: 378 NVKPIIIGKAPSCS 391
NVKP IIGKAP+C+
Sbjct: 276 NVKPTIIGKAPTCA 235
>AV408303
Length = 362
Score = 28.1 bits (61), Expect = 2.6
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = +3
Query: 363 ISLTFNGAPAMAKCANVKPIIIGK 386
++LTFN + AK ++KP I+GK
Sbjct: 9 LALTFNKSSRFAKLQSIKPAIVGK 80
>TC11950 similar to UP|Q43573 (Q43573) Zn finger protein (Fragment), partial
(7%)
Length = 808
Score = 27.7 bits (60), Expect = 3.4
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = -1
Query: 230 ITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTW 279
I IH+ CG H F I NP + N+ +K C +T+ I+ W
Sbjct: 229 IVIHSC-CGARHLFQILKREQNPPSTCILNMTLKTCIITSIPI*SEIQGW 83
>BI418523
Length = 496
Score = 27.7 bits (60), Expect = 3.4
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = +1
Query: 77 PCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTL-SGNG 120
P I+ VDG Q P+ + G F+ + YF+L SGNG
Sbjct: 196 PSAGFIKCNVDGASQGNPGPSGVGGVFRDANRKILGYFSLNSGNG 330
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,881,767
Number of Sequences: 28460
Number of extensions: 92062
Number of successful extensions: 431
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 417
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 419
length of query: 392
length of database: 4,897,600
effective HSP length: 92
effective length of query: 300
effective length of database: 2,279,280
effective search space: 683784000
effective search space used: 683784000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0490.17